qubit dsdna hs assay kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Qubit dsDNA HS Assay Kit
    Description:
    The Qubit dsDNA HS High Sensitivity Assay Kit is designed specifically for use with the Qubit Fluorometer The assay is highly selective for double stranded DNA dsDNA over RNA and is designed to be accurate for initial sample concentrations from 10 pg µL to 100 ng µL The kit provides concentrated assay reagent dilution buffer and pre diluted DNA standards Simply dilute the reagent using the buffer provided add your sample any volume between 1 µl and 20 µl is acceptable and read the concentration using the Qubit Fluorometer Common contaminants such as salts free nucleotides solvents detergents or protein are well tolerated in the assay Which product to choose for dsDNA HS High Sensitivity quantitation • For 1 20 samples use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer • For 20 2000 samples use the Quant iT dsDNA HS Assay Kit with microplate readerNotes 1 All Qubit assay kits can be used with the Qubit 1 0 Qubit 2 0 Qubit 3 and Qubit 4 fluorometers 2 500 µL thin walled PCR tubes are required but not included 3 Also available as a 1X dsDNA assay kit that provides reagent and buffer in a formulation that is stable as a ready to use solution for up to one year after preparation
    Catalog Number:
    q32851
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Quantitation|Nucleic Acid Quantitation
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher qubit dsdna hs assay kit
    Relative concentrations of reference materials (RMs) among the NanoDrop One C , <t>Qubit</t> <t>dsDNA</t> HS Assay and ddPCR using SET1 and SET2. Concentrations were measured twice at days 1 ( A ) and 2 ( B ). Values of NanoDrop, Qubit and droplet digital PCR (ddPCR) SET2 were calculated for a SET 1 ddPCR value of 1.
    The Qubit dsDNA HS High Sensitivity Assay Kit is designed specifically for use with the Qubit Fluorometer The assay is highly selective for double stranded DNA dsDNA over RNA and is designed to be accurate for initial sample concentrations from 10 pg µL to 100 ng µL The kit provides concentrated assay reagent dilution buffer and pre diluted DNA standards Simply dilute the reagent using the buffer provided add your sample any volume between 1 µl and 20 µl is acceptable and read the concentration using the Qubit Fluorometer Common contaminants such as salts free nucleotides solvents detergents or protein are well tolerated in the assay Which product to choose for dsDNA HS High Sensitivity quantitation • For 1 20 samples use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer • For 20 2000 samples use the Quant iT dsDNA HS Assay Kit with microplate readerNotes 1 All Qubit assay kits can be used with the Qubit 1 0 Qubit 2 0 Qubit 3 and Qubit 4 fluorometers 2 500 µL thin walled PCR tubes are required but not included 3 Also available as a 1X dsDNA assay kit that provides reagent and buffer in a formulation that is stable as a ready to use solution for up to one year after preparation
    https://www.bioz.com/result/qubit dsdna hs assay kit/product/Thermo Fisher
    Average 99 stars, based on 1062 article reviews
    Price from $9.99 to $1999.99
    qubit dsdna hs assay kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Microfluidic Quantitative PCR Detection of 12 Transgenes from Horse Plasma for Gene Doping Control"

    Article Title: Microfluidic Quantitative PCR Detection of 12 Transgenes from Horse Plasma for Gene Doping Control

    Journal: Genes

    doi: 10.3390/genes11040457

    Relative concentrations of reference materials (RMs) among the NanoDrop One C , Qubit dsDNA HS Assay and ddPCR using SET1 and SET2. Concentrations were measured twice at days 1 ( A ) and 2 ( B ). Values of NanoDrop, Qubit and droplet digital PCR (ddPCR) SET2 were calculated for a SET 1 ddPCR value of 1.
    Figure Legend Snippet: Relative concentrations of reference materials (RMs) among the NanoDrop One C , Qubit dsDNA HS Assay and ddPCR using SET1 and SET2. Concentrations were measured twice at days 1 ( A ) and 2 ( B ). Values of NanoDrop, Qubit and droplet digital PCR (ddPCR) SET2 were calculated for a SET 1 ddPCR value of 1.

    Techniques Used: Digital PCR

    2) Product Images from "Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application"

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    Journal: BMC Genomics

    doi: 10.1186/s12864-017-4371-5

    Storage condition of purified ChIP DNA is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; LoBind, Eppendorf DNA LoBind Snap Cap PCR Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Figure Legend Snippet: Storage condition of purified ChIP DNA is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; LoBind, Eppendorf DNA LoBind Snap Cap PCR Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube

    Techniques Used: Purification, Chromatin Immunoprecipitation, Concentration Assay, Sensitive Assay, Polymerase Chain Reaction

    3) Product Images from "Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study"

    Article Title: Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study

    Journal: Oncotarget

    doi: 10.18632/oncotarget.21256

    Optimization of circulating free DNA (cfDNA) extraction and quantification of cfDNA in the samples (A) Reproducibility of cfDNA extraction using the QIAamp Circulating Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA) on two independent cfDNA samples extracted from 1 mL (Ai) and 3 mL (Aii) of plasma from NSCLC patients. After extraction, cfDNA was quantified by Qubit dsDNA HS Assay Kit (Life Technologies, Q32854, Carlsbad, CA, USA) according to the manufacturer's instructions. (B) Correlation between the initial volume of plasma 1 mL versus 3 mL (Bi) or 3 mL versus 5 mL (Bii) and the quantity of cfDNA extracted (in ng/μL). (Ci) Fragment size visualization of cfDNA (in bp) from a concentrated (left) and a less concentrated (right) sample obtained using the Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) (Cii) , and average size distribution (10 bp increments) of cfDNA fragments in 77 plasma samples. (D) Correlation between cfDNA concentration measured using the Qubit method and the number of amplifiable copies in the corresponding plasma samples determined using the Quantifiler Kit.
    Figure Legend Snippet: Optimization of circulating free DNA (cfDNA) extraction and quantification of cfDNA in the samples (A) Reproducibility of cfDNA extraction using the QIAamp Circulating Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA) on two independent cfDNA samples extracted from 1 mL (Ai) and 3 mL (Aii) of plasma from NSCLC patients. After extraction, cfDNA was quantified by Qubit dsDNA HS Assay Kit (Life Technologies, Q32854, Carlsbad, CA, USA) according to the manufacturer's instructions. (B) Correlation between the initial volume of plasma 1 mL versus 3 mL (Bi) or 3 mL versus 5 mL (Bii) and the quantity of cfDNA extracted (in ng/μL). (Ci) Fragment size visualization of cfDNA (in bp) from a concentrated (left) and a less concentrated (right) sample obtained using the Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) (Cii) , and average size distribution (10 bp increments) of cfDNA fragments in 77 plasma samples. (D) Correlation between cfDNA concentration measured using the Qubit method and the number of amplifiable copies in the corresponding plasma samples determined using the Quantifiler Kit.

    Techniques Used: Concentration Assay

    4) Product Images from "Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq"

    Article Title: Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq

    Journal: BMC Genomics

    doi: 10.1186/s12864-019-5639-8

    A tissue-level cross-link reversal allows efficient extraction of high-quality chromatin from FFPE tissues. a Schematic diagram of chromatin extraction method from FFPE tissues (ChromEX-PE). A tissue-level, cross-link reversal was introduced by incubating deparaffinized tissue in the range of temperature from 25 to 75 °C before chromatin extraction. b Chrom-EX PE dramatically increases soluble chromatin from FFPE tissues. Chromatin was prepared by Chrom-EX PE with 65 °C overnight incubation or without incubation. A commercial kit from Active motif was used as control and for comparison. DNAs were purified from soluble fraction and insoluble pellet fraction and were quantified using Qubit dsDNA High Sensitivity assay. The percentage of soluble chromatin was calculated from mouse spleen, kidney, and liver FFPE tissues. The data were generated from two independent experiments. c Chrom-EX PE generates different sizes of chromatin in a controlled manner. Chromatin was prepared from mouse liver FFPE tissues by Chrom-EX PE in the range of temperatures from 25 °C to 75 °C. Deparaffinized tissue was incubated in the indicated temperature overnight in the chromatin stabilization buffer. In the range of 25–37 °C, a majority of soluble chromatin is larger than 1 kb. The temperature ranges (45–55 °C) produce nucleosomal DNA patterns. The majority of DNA was mononucleosomal DNA at 60 °C incubation and DNA size is closer or smaller than mononucleosomal DNA with temperature above 60 °C. d Chromatin yield by Chrom-EX PE from various mouse tissues. The tissue volume is measured in FFPE block and two 20-μm sections were processed by Chrom-EX PE at 65 °C condition with chromatin stabilization buffer. The DNA amount purified from soluble fraction was measured and the yield was calculated per tissue volume
    Figure Legend Snippet: A tissue-level cross-link reversal allows efficient extraction of high-quality chromatin from FFPE tissues. a Schematic diagram of chromatin extraction method from FFPE tissues (ChromEX-PE). A tissue-level, cross-link reversal was introduced by incubating deparaffinized tissue in the range of temperature from 25 to 75 °C before chromatin extraction. b Chrom-EX PE dramatically increases soluble chromatin from FFPE tissues. Chromatin was prepared by Chrom-EX PE with 65 °C overnight incubation or without incubation. A commercial kit from Active motif was used as control and for comparison. DNAs were purified from soluble fraction and insoluble pellet fraction and were quantified using Qubit dsDNA High Sensitivity assay. The percentage of soluble chromatin was calculated from mouse spleen, kidney, and liver FFPE tissues. The data were generated from two independent experiments. c Chrom-EX PE generates different sizes of chromatin in a controlled manner. Chromatin was prepared from mouse liver FFPE tissues by Chrom-EX PE in the range of temperatures from 25 °C to 75 °C. Deparaffinized tissue was incubated in the indicated temperature overnight in the chromatin stabilization buffer. In the range of 25–37 °C, a majority of soluble chromatin is larger than 1 kb. The temperature ranges (45–55 °C) produce nucleosomal DNA patterns. The majority of DNA was mononucleosomal DNA at 60 °C incubation and DNA size is closer or smaller than mononucleosomal DNA with temperature above 60 °C. d Chromatin yield by Chrom-EX PE from various mouse tissues. The tissue volume is measured in FFPE block and two 20-μm sections were processed by Chrom-EX PE at 65 °C condition with chromatin stabilization buffer. The DNA amount purified from soluble fraction was measured and the yield was calculated per tissue volume

    Techniques Used: Formalin-fixed Paraffin-Embedded, Incubation, Purification, Sensitive Assay, Generated, Blocking Assay

    5) Product Images from "Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies"

    Article Title: Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies

    Journal: Genome Medicine

    doi: 10.1186/gm481

    FFPE DNA characterization by QFI-PCR and fluorescence-based assays from 165 tumor DNA samples. (A) Distribution of FFPE DNA quantification using QFI-PCR and the fluorescence-based Qubit dsDNA HS assay from 5 ng bulk DNA input as determined by NanoDrop spectrophotometry. A total of 27 samples were undetected by fluorescence assay (
    Figure Legend Snippet: FFPE DNA characterization by QFI-PCR and fluorescence-based assays from 165 tumor DNA samples. (A) Distribution of FFPE DNA quantification using QFI-PCR and the fluorescence-based Qubit dsDNA HS assay from 5 ng bulk DNA input as determined by NanoDrop spectrophotometry. A total of 27 samples were undetected by fluorescence assay (

    Techniques Used: Formalin-fixed Paraffin-Embedded, Polymerase Chain Reaction, Fluorescence, Spectrophotometry

    6) Product Images from "Nucleic Acid Extraction from Synthetic Mars Analog Soils for in situ Life Detection"

    Article Title: Nucleic Acid Extraction from Synthetic Mars Analog Soils for in situ Life Detection

    Journal: Astrobiology

    doi: 10.1089/ast.2016.1535

    Random hexamer primer detection by a dsDNA-specific fluorometric assay. Random hexamers (250, 500, 1000, 2000, 4000 ng) were added to 200 μL of the PureLyse elution buffer, homogenized, then quantified on a Qubit 2.0 fluorometer (standard error shown, n = 3). Random hexamer loads were not detectable until 20 ng/μL (4000 ng in 200 μL), likely due to increased hybridization. These results suggest that using random hexamer primers (less than 20 ng/μL) as competitive binders will not bias DNA yield results with this dsDNA-specific fluorometric assay. *At or below 0.01 ng limit of detection.
    Figure Legend Snippet: Random hexamer primer detection by a dsDNA-specific fluorometric assay. Random hexamers (250, 500, 1000, 2000, 4000 ng) were added to 200 μL of the PureLyse elution buffer, homogenized, then quantified on a Qubit 2.0 fluorometer (standard error shown, n = 3). Random hexamer loads were not detectable until 20 ng/μL (4000 ng in 200 μL), likely due to increased hybridization. These results suggest that using random hexamer primers (less than 20 ng/μL) as competitive binders will not bias DNA yield results with this dsDNA-specific fluorometric assay. *At or below 0.01 ng limit of detection.

    Techniques Used: Random Hexamer Labeling, Hybridization

    7) Product Images from "DNA extraction approaches substantially influence the assessment of the human breast milk microbiome"

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-55568-y

    DNA concentration (ng/µL) of mock breast milk ( a ) and human breast milk samples ( b ) as determined by Qubit dsDNA HS Assay kit. The dashed line represents the expected DNA concentration of 5.49 ng/uL, based on the bacteria added to the mock breast milk. Expected concentration was calculated based on the size of each bacterial genome, the weight of a base pair, and the CFU of each bacteria. *P
    Figure Legend Snippet: DNA concentration (ng/µL) of mock breast milk ( a ) and human breast milk samples ( b ) as determined by Qubit dsDNA HS Assay kit. The dashed line represents the expected DNA concentration of 5.49 ng/uL, based on the bacteria added to the mock breast milk. Expected concentration was calculated based on the size of each bacterial genome, the weight of a base pair, and the CFU of each bacteria. *P

    Techniques Used: Concentration Assay

    Related Articles

    Fluorescence:

    Article Title: Validating DNA Extraction Protocols for Bentonite Clay
    Article Snippet: .. Genomic DNA concentration was determined using the Qubit dsDNA High Sensitivity (HS) assay kit (Invitrogen, Carlsbad, CA, USA) with fluorescence measured on a FilterMax F5 MultiMode plate reader (Molecular Devices, San Jose, CA, USA) at excitation and emission wavelengths of 485 and 525 nm, respectively. .. The quality of extracted E. coli DNA was assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) and pulsed-field gel electrophoresis (PFGE) using a CHEF Mapper XA apparatus (Bio-Rad, Hercules, CA, USA).

    Sensitive Assay:

    Article Title: Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq
    Article Snippet: .. DNAs were purified using Min-Elute PCR purification kit after the treatment of RNase A and proteinase K. DNA amount was measured by the Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851), and DNA size was analyzed by the Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. #DNF-486). .. For the comparison of soluble chromatin yield, the ChIP-IT® FFPE Chromatin Preparation Kit (Active Motif, Cat # 53030) was utilized according to the manufacture’s instructions.

    Article Title: Maintaining Breast Cancer Specimen Integrity and Individual or Simultaneous Extraction of Quality DNA, RNA, and Proteins from Allprotect-Stabilized and Nonstabilized Tissue Samples
    Article Snippet: .. DNA was quantified using Qubit [also known as Quant-iT™ dsDNA HS (High Sensitivity) Assay Kit; Invitrogen], Nanodrop 2000™, Thermo Scientific (designated Nanodrop1), and NanoDrop ND-1000™, NanoDrop Technologies (designated Nanodrop2), as per manufacturer's instructions. .. Both the ratio of absorbance at 260 and 280 nm (A260/280 ) and gel electrophoresis were utilized to ascertain the quality and purity of DNA isolates.

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    Polymerase Chain Reaction:

    Article Title: Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq
    Article Snippet: .. DNAs were purified using Min-Elute PCR purification kit after the treatment of RNase A and proteinase K. DNA amount was measured by the Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851), and DNA size was analyzed by the Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. #DNF-486). .. For the comparison of soluble chromatin yield, the ChIP-IT® FFPE Chromatin Preparation Kit (Active Motif, Cat # 53030) was utilized according to the manufacture’s instructions.

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    Next-Generation Sequencing:

    Article Title: Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq
    Article Snippet: .. DNAs were purified using Min-Elute PCR purification kit after the treatment of RNase A and proteinase K. DNA amount was measured by the Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851), and DNA size was analyzed by the Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. #DNF-486). .. For the comparison of soluble chromatin yield, the ChIP-IT® FFPE Chromatin Preparation Kit (Active Motif, Cat # 53030) was utilized according to the manufacture’s instructions.

    Purification:

    Article Title: Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq
    Article Snippet: .. DNAs were purified using Min-Elute PCR purification kit after the treatment of RNase A and proteinase K. DNA amount was measured by the Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851), and DNA size was analyzed by the Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. #DNF-486). .. For the comparison of soluble chromatin yield, the ChIP-IT® FFPE Chromatin Preparation Kit (Active Motif, Cat # 53030) was utilized according to the manufacture’s instructions.

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    Concentration Assay:

    Article Title: Validating DNA Extraction Protocols for Bentonite Clay
    Article Snippet: .. Genomic DNA concentration was determined using the Qubit dsDNA High Sensitivity (HS) assay kit (Invitrogen, Carlsbad, CA, USA) with fluorescence measured on a FilterMax F5 MultiMode plate reader (Molecular Devices, San Jose, CA, USA) at excitation and emission wavelengths of 485 and 525 nm, respectively. .. The quality of extracted E. coli DNA was assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) and pulsed-field gel electrophoresis (PFGE) using a CHEF Mapper XA apparatus (Bio-Rad, Hercules, CA, USA).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies
    Article Snippet: .. DNA quantification using the Qubit® 2.0 Fluorometer A total of 165 FFPE samples were diluted to 5 ng/μl and quantified using the Qubit dsDNA HS Assay Kit (catalogue number Q32854, Life Technologies) and the Qubit 2.0 Fluorometer (Life Technologies) per the manufacturer’s instruction. .. Briefly, 1 μl of DNA sample at 5 ng/μl was diluted 200-fold in Qubit dsDNA HS buffer in clear plastic Qubit Assay Tubes (catalogue number Q32856, Life Technologies) and measured on the fluorometer.

    IA:

    Article Title: Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq
    Article Snippet: .. DNAs were purified using Min-Elute PCR purification kit after the treatment of RNase A and proteinase K. DNA amount was measured by the Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851), and DNA size was analyzed by the Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. #DNF-486). .. For the comparison of soluble chromatin yield, the ChIP-IT® FFPE Chromatin Preparation Kit (Active Motif, Cat # 53030) was utilized according to the manufacture’s instructions.

    other:

    Article Title: Biases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples
    Article Snippet: DNA yield was measured using two methods: Nanodrop (Nanodrop One, Thermoscientific, USA) and the Qubit fluorometer (Invitrogen, USA, using the Qubit® dsDNA HS Assay Kit).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher qubit dsdna hs assay kit
    Relative concentrations of reference materials (RMs) among the NanoDrop One C , <t>Qubit</t> <t>dsDNA</t> HS Assay and ddPCR using SET1 and SET2. Concentrations were measured twice at days 1 ( A ) and 2 ( B ). Values of NanoDrop, Qubit and droplet digital PCR (ddPCR) SET2 were calculated for a SET 1 ddPCR value of 1.
    Qubit Dsdna Hs Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1062 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit dsdna hs assay kit/product/Thermo Fisher
    Average 94 stars, based on 1062 article reviews
    Price from $9.99 to $1999.99
    qubit dsdna hs assay kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Relative concentrations of reference materials (RMs) among the NanoDrop One C , Qubit dsDNA HS Assay and ddPCR using SET1 and SET2. Concentrations were measured twice at days 1 ( A ) and 2 ( B ). Values of NanoDrop, Qubit and droplet digital PCR (ddPCR) SET2 were calculated for a SET 1 ddPCR value of 1.

    Journal: Genes

    Article Title: Microfluidic Quantitative PCR Detection of 12 Transgenes from Horse Plasma for Gene Doping Control

    doi: 10.3390/genes11040457

    Figure Lengend Snippet: Relative concentrations of reference materials (RMs) among the NanoDrop One C , Qubit dsDNA HS Assay and ddPCR using SET1 and SET2. Concentrations were measured twice at days 1 ( A ) and 2 ( B ). Values of NanoDrop, Qubit and droplet digital PCR (ddPCR) SET2 were calculated for a SET 1 ddPCR value of 1.

    Article Snippet: Quantitation of Transgenes Cloned into Plasmid Vector The 12 transgenes dissolved in Milli-Q water were quantified using a NanoDrop OneC , Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and droplet digital PCR (ddPCR; Bio-Rad, Hercules, CA, USA) using a TaqMan probe.

    Techniques: Digital PCR