qubit dsdna br assay kit  (Thermo Fisher)


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    Name:
    Qubit dsDNA BR Assay Kit
    Description:
    The Qubit dsDNA BR Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.Which product to choose for dsDNA BR (Broad Range) quantitation? • For 1–20 samples: use this Qubit dsDNA BR Assay Kit with the Qubit Fluorometer • For 20–2000 samples: use the Quant-iT dsDNA BR Assay Kit with microplate readerNotes:1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.2. 500 µL thin-walled PCR tubes are required but not included.
    Catalog Number:
    Q32850
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Quantitation|Nucleic Acid Quantitation
    Size:
    100 assays
    Category:
    Kits and Assays, DNA⁄RNA Quantitation Assay Kits
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher qubit dsdna br assay kit
    3.2.5.1. <t>Qubit®</t> <t>dsDNA</t> BR Assay
    The Qubit dsDNA BR Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.Which product to choose for dsDNA BR (Broad Range) quantitation? • For 1–20 samples: use this Qubit dsDNA BR Assay Kit with the Qubit Fluorometer • For 20–2000 samples: use the Quant-iT dsDNA BR Assay Kit with microplate readerNotes:1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.2. 500 µL thin-walled PCR tubes are required but not included.
    https://www.bioz.com/result/qubit dsdna br assay kit/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    qubit dsdna br assay kit - by Bioz Stars, 2019-10
    99/100 stars

    Images

    1) Product Images from "RNA sequencing analysis of salivary extracellular RNA"

    Article Title: RNA sequencing analysis of salivary extracellular RNA

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-4939-6685-1_2

    3.2.5.1. Qubit® dsDNA BR Assay
    Figure Legend Snippet: 3.2.5.1. Qubit® dsDNA BR Assay

    Techniques Used:

    2) Product Images from "FabV/Triclosan Is an Antibiotic-Free and Cost-Effective Selection System for Efficient Maintenance of High and Medium -Copy Number Plasmids in Escherichia coli"

    Article Title: FabV/Triclosan Is an Antibiotic-Free and Cost-Effective Selection System for Efficient Maintenance of High and Medium -Copy Number Plasmids in Escherichia coli

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0129547

    Plasmid DNA yield. E . coli DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV, pUC19-FabI, and medium-copy number pSA-Hp24-Bla, pSA-Hp24-FabV, pBR322-FabI plasmids and selected on LB agar plates containing 1μM Triclosan (for FabV/FabI plasmids) and or 100μg mL -1 ampicillin (for Bla plasmids). Seed cultures were used to inoculated 5mL LB broth in 50mL flasks and cultured for another 18 hours at 37 or 30°C while shaking at 250rpm. Cell density was then measured by absorbing the diluted samples at 600nm and normalized to 2 OD600. One mL of the normalized culture was then used to extract the plasmid following supplied protocol. The quantity of the extracted plasmid DNA was measured by fluorometry using Qubit Fluorometer and Qubit dsDNA BR Assay Kit (Invitrogen, life technology). Fig 4A shows plasmid DNA yield when bacteria were grown at 37°C, whereas Fig 4B shows the plasmid DNA yield from cultures incubated at 30°C. Error bars show standard deviations calculated from at-least six (6) independent experiments performed in triplicate.
    Figure Legend Snippet: Plasmid DNA yield. E . coli DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV, pUC19-FabI, and medium-copy number pSA-Hp24-Bla, pSA-Hp24-FabV, pBR322-FabI plasmids and selected on LB agar plates containing 1μM Triclosan (for FabV/FabI plasmids) and or 100μg mL -1 ampicillin (for Bla plasmids). Seed cultures were used to inoculated 5mL LB broth in 50mL flasks and cultured for another 18 hours at 37 or 30°C while shaking at 250rpm. Cell density was then measured by absorbing the diluted samples at 600nm and normalized to 2 OD600. One mL of the normalized culture was then used to extract the plasmid following supplied protocol. The quantity of the extracted plasmid DNA was measured by fluorometry using Qubit Fluorometer and Qubit dsDNA BR Assay Kit (Invitrogen, life technology). Fig 4A shows plasmid DNA yield when bacteria were grown at 37°C, whereas Fig 4B shows the plasmid DNA yield from cultures incubated at 30°C. Error bars show standard deviations calculated from at-least six (6) independent experiments performed in triplicate.

    Techniques Used: Plasmid Preparation, Transformation Assay, Cell Culture, Incubation

    Related Articles

    Amplification:

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: Selected fragments were treated with sodium bisulfite to convert unmethylated cytosines to uracils, which are then replaced with thymines during PCR amplification. .. After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Paragraph title: Next-generation eDNA amplicon sequencing ... DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 .

    Article Title: CRISPR/Cas9 System as a Valuable Genome Editing Tool for Wine Yeasts with Application to Decrease Urea Production
    Article Snippet: The amplification cycle was an initial denaturation at 95°C for 5 min, 45 s at the annealing temperature (3°C lower than melting temperature) and 1.5 min at 72°C for the extension. .. Bands were extracted from gel, eluted in 50 μL of mQ water using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and quantified by Qubit® dsDNA BR (Broad-Range) Assay Kits (Invitrogen).

    Positive Control:

    Article Title: Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant, et al. Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant
    Article Snippet: DNA was quantified using a Qubit Broad Range dsDNA Assay (ThermoFisher Scientific) according to the manufacturer's instructions and normalized to c. 7 ng/μl. .. DNA was quantified using a Qubit Broad Range dsDNA Assay (ThermoFisher Scientific) according to the manufacturer's instructions and normalized to c. 7 ng/μl.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 . .. DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 .

    Synthesized:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: Messenger RNA isolation and cDNA synthesis were performed using TruSeq RNA Sample Preparation Kit (Illumina, USA) and Super Script II Reverse Transcriptase (Invitrogen, USA). .. The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA). .. A minimum of 40ng cDNA was fragmented using Covaris S220 (Covaris INC, USA) to a targeted size of 200–300 bp.

    Lambda DNA Preparation:

    Article Title: Evolution of DNA Methylation Patterns in the Brassicaceae is Driven by Differences in Genome Organization
    Article Snippet: DNA was quantified using the Qubit BR assay (Life Technologies, catalog # Q32853). .. DNA was quantified using the Qubit BR assay (Life Technologies, catalog # Q32853).

    Construct:

    Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
    Article Snippet: Each library was constructed using ∼4 μg of purified DNA and the SMRTbell Template Prep Kit 1.0, according to the protocol described in ‘Procedure & Checklist—20 kb Template Preparation Using BluePippin™ Size-Selection System’ (Pacific Biosciences). .. Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

    SYBR Green Assay:

    Article Title: Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia
    Article Snippet: RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat # ) and Invitrogen Qubit 2.0 Fluorometer (Cat # ). .. RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat # ) and Invitrogen Qubit 2.0 Fluorometer (Cat # ).

    Incubation:

    Article Title: Dynamics and Determinants of HPV Infection: The Michigan HPV and Oropharyngeal Cancer (M-HOC) Study
    Article Snippet: The DNA pellet is rehydrated with Tris–EDTA buffer and quantified with picogreen (Invitrogen P11496) or the QuBit (Invitrogen Q32850). .. The DNA pellet is rehydrated with Tris–EDTA buffer and quantified with picogreen (Invitrogen P11496) or the QuBit (Invitrogen Q32850).

    Expressing:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: The results of immunohistochemical staining for MMR protein expression, such as MLH1 and MSH2, revealed that 13 of these tumors exhibited defective DNA MMR, while 39 presented proficient MMR proteins. .. DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA).

    Modification:

    Article Title: A striking new species of leaf warbler from the Lesser Sundas as uncovered through morphology and genomics
    Article Snippet: We prepared double-digest restriction-site associated DNA sequencing (ddRADSeq) libraries using a modified version of Peterson et al .‘s protocol, as described by Garg et al . .. Final concentration and fragment size checks on the library were performed using a Qubit 2.0 Broad Range DNA assay (Invitrogen, California, USA) and a Fragment Analyser (Advanced Analytical Technologies, Iowa, USA), respectively.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA). .. The fragmented cDNA was then end-repaired, ligated to Illumina TruSeq adaptors, and PCR-enriched using TruSeq RNA sample preparation kit (Illumina, USA).

    Article Title: Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia
    Article Snippet: Paragraph title: RNA extraction and quantitative PCR ... RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat # ) and Invitrogen Qubit 2.0 Fluorometer (Cat # ).

    Article Title: Molecular Preadaptation to Antimony Resistance in Leishmania donovani on the Indian Subcontinent
    Article Snippet: DNA isolation was done using QIAamp DNA blood minikit (Qiagen), and the DNA concentration was assessed with the Qubit DNA broad-range DNA quantification kit (Thermo Fisher). .. Sequencing libraries were prepared with the TruSeq DNA library prep kit (Illumina) according to the manufacturer’s instructions.

    Immunohistochemistry:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: The results of immunohistochemical staining for MMR protein expression, such as MLH1 and MSH2, revealed that 13 of these tumors exhibited defective DNA MMR, while 39 presented proficient MMR proteins. .. DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA).

    Gas Chromatography:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: 2.1 A total of 52 primary gastric tumor tissues and paired normal tissues were obtained from surgically dissected patients with GC between 1999 to 2016 at the Daejeon St. Mary's Hospital, Daejeon, Republic of Korea. .. DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA).

    Ligation:

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: A single adenine nucleotide was next added to the 3′ end of the fragments in preparation for the ligation of the adapter duplex with a thymine overhang. .. After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Article Title: SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)
    Article Snippet: Input genomic DNA (gDNA) concentration was measured on a Qubit Fluorometer dsDNA Broad Range (Life Technologies, cat. no. 32850). .. Input genomic DNA (gDNA) concentration was measured on a Qubit Fluorometer dsDNA Broad Range (Life Technologies, cat. no. 32850).

    Article Title: Evolution of DNA Methylation Patterns in the Brassicaceae is Driven by Differences in Genome Organization
    Article Snippet: DNA was quantified using the Qubit BR assay (Life Technologies, catalog # Q32853). .. DNA was quantified using the Qubit BR assay (Life Technologies, catalog # Q32853).

    Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
    Article Snippet: Following ligation, extraneous DNA was digested with exonucleases and the SMRTbell library was cleaned and concentrated with AMPure PB beads. .. Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

    Methylation:

    Article Title: Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
    Article Snippet: DNA concentration was measured by the Qubit dsDNA Broad-Range Assay (Invitrogen, Q32850). .. DNA concentration was measured by the Qubit dsDNA Broad-Range Assay (Invitrogen, Q32850).

    Generated:

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Positive controls were generated using genomic DNA from four Sanger sequenced, vouchered harbour porpoise samples to test for PCR or sequencing error. .. DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 .

    DNA Sequencing:

    Article Title: A striking new species of leaf warbler from the Lesser Sundas as uncovered through morphology and genomics
    Article Snippet: We prepared double-digest restriction-site associated DNA sequencing (ddRADSeq) libraries using a modified version of Peterson et al .‘s protocol, as described by Garg et al . .. Final concentration and fragment size checks on the library were performed using a Qubit 2.0 Broad Range DNA assay (Invitrogen, California, USA) and a Fragment Analyser (Advanced Analytical Technologies, Iowa, USA), respectively.

    Polymerase Chain Reaction:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA). .. The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA).

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: Selected fragments were treated with sodium bisulfite to convert unmethylated cytosines to uracils, which are then replaced with thymines during PCR amplification. .. After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Article Title: Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia
    Article Snippet: RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat # ) and Invitrogen Qubit 2.0 Fluorometer (Cat # ). .. BioRad iTaq Universal SYBR Green Supermix (Cat #1725121) and PrimePCR SYBR Green Assay primers were used for all qPCR reactions: STAT1 (Cat #10025636, qHsaCED0043612), IFNGR1 (Cat #10025636, qHsaCID0013339), IFNGR2 (Cat #10025636, qHsaCID0008956), IRF-1 (Cat #10025636, qHsaCED0044080), SOCS1 (Cat #10025636, qHsaCED0002534), IFNG (Cat #10025636, qHsaCID0017614), UBC (Cat #10025636, qHsaCED0023867).

    Article Title: Evaluating the Performance of De Novo Assembly Methods for Venom-Gland Transcriptomics
    Article Snippet: The concentration of the RNA was determined with the Qubit Broad Range RNA assay (Thermo-Fisher Scientific, Waltham, MA, USA) and quality of the extraction was checked on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. The concentration of the RNA was determined with the Qubit Broad Range RNA assay (Thermo-Fisher Scientific, Waltham, MA, USA) and quality of the extraction was checked on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Positive controls were generated using genomic DNA from four Sanger sequenced, vouchered harbour porpoise samples to test for PCR or sequencing error. .. DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 .

    Staining:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: The results of immunohistochemical staining for MMR protein expression, such as MLH1 and MSH2, revealed that 13 of these tumors exhibited defective DNA MMR, while 39 presented proficient MMR proteins. .. DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA).

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 . .. Each sample was amplified in three independent 10 µl reactions to ensure sufficient PCR product for next-generation sequencing.

    DNA Extraction:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: Paragraph title: Samples and DNA isolation ... DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA).

    Article Title: Tracking the evolution of epialleles during neural differentiation and brain development: D-Aspartate oxidase as a model gene
    Article Snippet: Paragraph title: DNA extraction from cells and tissues ... DNA was quality checked by 260/280 absorbance ratio using NanoDrop 2000, (Thermo Scientific) and it was quantified using Qubit® 2.0 Fluorometer with the dsDNA broad range assay kit (Invitrogen, Q32850).

    Article Title: Evolution of DNA Methylation Patterns in the Brassicaceae is Driven by Differences in Genome Organization
    Article Snippet: Paragraph title: DNA extraction and bisulfite library preparation ... DNA was quantified using the Qubit BR assay (Life Technologies, catalog # Q32853).

    Article Title: Dynamics and Determinants of HPV Infection: The Michigan HPV and Oropharyngeal Cancer (M-HOC) Study
    Article Snippet: DNA isolation from saliva samples is performed by first splitting the sample in half, half for DNA and half for RNA. .. The DNA pellet is rehydrated with Tris–EDTA buffer and quantified with picogreen (Invitrogen P11496) or the QuBit (Invitrogen Q32850).

    Article Title: Molecular Preadaptation to Antimony Resistance in Leishmania donovani on the Indian Subcontinent
    Article Snippet: Parasites were pelleted after 5 weeks of culture with a given concentration of SbIII . .. DNA isolation was done using QIAamp DNA blood minikit (Qiagen), and the DNA concentration was assessed with the Qubit DNA broad-range DNA quantification kit (Thermo Fisher). .. Sequencing libraries were prepared with the TruSeq DNA library prep kit (Illumina) according to the manufacturer’s instructions.

    Article Title: Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
    Article Snippet: Paragraph title: DNA extraction and MBD-Seq library preparation ... DNA concentration was measured by the Qubit dsDNA Broad-Range Assay (Invitrogen, Q32850).

    Magnetic Beads:

    Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
    Article Snippet: Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher). .. To identify the library concentration that would achieve optimal Poisson loading on the SMRT Cell (i.e. ∼40% of zero mode waveguides loaded with a single DNA molecule), loading titrations were performed for each library.

    Article Title: A striking new species of leaf warbler from the Lesser Sundas as uncovered through morphology and genomics
    Article Snippet: We used the restriction enzymes EcoRI and MspI, and performed DNA fragment cleanup steps with Sera-Mag magnetic beads (Thermo-Scientific, Fremont, California). .. Final concentration and fragment size checks on the library were performed using a Qubit 2.0 Broad Range DNA assay (Invitrogen, California, USA) and a Fragment Analyser (Advanced Analytical Technologies, Iowa, USA), respectively.

    Isolation:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: Messenger RNA isolation and cDNA synthesis were performed using TruSeq RNA Sample Preparation Kit (Illumina, USA) and Super Script II Reverse Transcriptase (Invitrogen, USA). .. The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA).

    Article Title: Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940
    Article Snippet: High-molecular-weight genomic DNA was isolated from M. elsdenii ATCC 25940 and NCIMB 702410 cultures using a Genomic-tip 100/G kit (Qiagen, Valencia, CA), following the manufacturer’s protocol. .. The final library was validated on a Bioanalyzer instrument (Agilent) using a DNA7500 chip, and concentration was determined using a Qubit double-stranded DNA (dsDNA) broad-range (BR) assay kit (Invitrogen).

    Article Title: Systematic biases in DNA copy number originate from isolation procedures
    Article Snippet: Paragraph title: Isolation of genomic DNA ... DNA quality and quantity of all isolations were measured using NanoDrop ND-1000 (Thermo scientific) and a Qubit Quant-iT™ dsDNA broad range assay (Invitrogen).

    Article Title: Dynamics and Determinants of HPV Infection: The Michigan HPV and Oropharyngeal Cancer (M-HOC) Study
    Article Snippet: The DNA pellet is rehydrated with Tris–EDTA buffer and quantified with picogreen (Invitrogen P11496) or the QuBit (Invitrogen Q32850). .. RNA is isolated from samples that have tested positive for HPV DNA.

    Article Title: Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia
    Article Snippet: For all experiments, cells were harvested and lysed with Invitrogen TRIzol Reagent (Cat #15596018) and stored at -80°C. .. RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat # ) and Invitrogen Qubit 2.0 Fluorometer (Cat # ). .. Clontech RNA to cDNA EcoDry Premix (Double Primed) (Cat #639548) was used to reverse transcribe the extracted RNA.

    Article Title: Next-generation sequencing of acute myeloid leukemia identifies the significance of TP53, U2AF1, ASXL1, and TET2 mutations
    Article Snippet: Genomic DNA was isolated from frozen blood or bone marrow aspirate samples using DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions and as previously described; in four cases, genomic DNA was isolated from Wright Giemsa-stained slides for repeat validation with similar results to frozen or fresh tissue. .. 125 ng of total genomic DNA was used and quantified using the Qubit DNA BR assay (Life Technologies, Carlsbad, CA, USA).

    Transfection:

    Article Title: Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia
    Article Snippet: For STAT knockdown experiments, TL-1 and Jurkat T cells were treated as described in the siRNA-mediated knockdown section and then RNA was extracted 48 h after siRNA transfection. .. RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat # ) and Invitrogen Qubit 2.0 Fluorometer (Cat # ).

    Purification:

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: The digested DNA was then purified using phenol:chloroform extraction and ethanol precipitation in the presence of glycogen, before blunt-ending and phosphorylation. .. After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
    Article Snippet: Each library was constructed using ∼4 μg of purified DNA and the SMRTbell Template Prep Kit 1.0, according to the protocol described in ‘Procedure & Checklist—20 kb Template Preparation Using BluePippin™ Size-Selection System’ (Pacific Biosciences). .. Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

    Article Title: Evaluating the Performance of De Novo Assembly Methods for Venom-Gland Transcriptomics
    Article Snippet: The concentration of the RNA was determined with the Qubit Broad Range RNA assay (Thermo-Fisher Scientific, Waltham, MA, USA) and quality of the extraction was checked on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. Total RNA from venom glands, with equimolar pooling of right and left venom gland RNA from the snakes, was used for mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), followed by the preparation of cDNA libraries using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) and Multiplex Oligos for Illumina (New England Biolabs), according to the manufacturer’s instructions.

    Sequencing:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: Paragraph title: RNA extraction, library preparation and sequencing ... The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA).

    Article Title: Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940
    Article Snippet: ATCC 25490 is also known as DSM 20460, for which a draft genome sequence is available under the GenBank accession number ( ). .. The final library was validated on a Bioanalyzer instrument (Agilent) using a DNA7500 chip, and concentration was determined using a Qubit double-stranded DNA (dsDNA) broad-range (BR) assay kit (Invitrogen).

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen). .. Single-end, 50 -bp reads were obtained for each library by sequencing on the HiSeq 4000 system (Illumina).

    Article Title: SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)
    Article Snippet: Paragraph title: SMRT library preparation and sequencing ... Input genomic DNA (gDNA) concentration was measured on a Qubit Fluorometer dsDNA Broad Range (Life Technologies, cat. no. 32850).

    Article Title: Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant, et al. Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant
    Article Snippet: DNA was quantified using a Qubit Broad Range dsDNA Assay (ThermoFisher Scientific) according to the manufacturer's instructions and normalized to c. 7 ng/μl. .. An additional positive control (repeated individual, LOC0279_d) was included to allow for quality control of the experimental process and for assessment of genotyping error‐by‐read depth.

    Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
    Article Snippet: Paragraph title: 2.4. SMRTbell library preparation and sequencing ... Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

    Article Title: Evaluating the Performance of De Novo Assembly Methods for Venom-Gland Transcriptomics
    Article Snippet: Paragraph title: 4.2. Tissue Preparation and Sequencing ... The concentration of the RNA was determined with the Qubit Broad Range RNA assay (Thermo-Fisher Scientific, Waltham, MA, USA) and quality of the extraction was checked on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: A striking new species of leaf warbler from the Lesser Sundas as uncovered through morphology and genomics
    Article Snippet: Paragraph title: Genomic Library Preparation and Sequencing ... Final concentration and fragment size checks on the library were performed using a Qubit 2.0 Broad Range DNA assay (Invitrogen, California, USA) and a Fragment Analyser (Advanced Analytical Technologies, Iowa, USA), respectively.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Paragraph title: Next-generation eDNA amplicon sequencing ... DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 .

    Lysis:

    Article Title: Systematic biases in DNA copy number originate from isolation procedures
    Article Snippet: For the additional proteinase K treatment experiment, 50% of the DNA that was extracted after a 10-min lysis was treated for an additional 120 min of proteinase K (100 μg/mL) in the lysis buffer described above. .. DNA quality and quantity of all isolations were measured using NanoDrop ND-1000 (Thermo scientific) and a Qubit Quant-iT™ dsDNA broad range assay (Invitrogen).

    Sample Prep:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: Messenger RNA isolation and cDNA synthesis were performed using TruSeq RNA Sample Preparation Kit (Illumina, USA) and Super Script II Reverse Transcriptase (Invitrogen, USA). .. The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA).

    Chromatin Immunoprecipitation:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: The RNA purity was checked using Agilent Bioanalyzer RNA Nano chip Bioanalyzer (Agilent Technologies, USA). .. The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA).

    Article Title: Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood
    Article Snippet: RNA integrity and purity were assayed using an RNA 6000 Pico chip on the Agilent Bioanalyzer™. .. DNA mass was determined using Qubit® Broad Range DNA kit (Life Technologies).

    Article Title: Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940
    Article Snippet: A Nextera XT library (Illumina, San Diego, CA) was prepared from M. elsdenii NCIMB 702410 genomic DNA, as described in the manufacturer’s protocol. .. The final library was validated on a Bioanalyzer instrument (Agilent) using a DNA7500 chip, and concentration was determined using a Qubit double-stranded DNA (dsDNA) broad-range (BR) assay kit (Invitrogen). .. Paired-end sequencing (2 × 301 bp) was completed using the Illumina MiSeq platform.

    Software:

    Article Title: Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940
    Article Snippet: The final library was validated on a Bioanalyzer instrument (Agilent) using a DNA7500 chip, and concentration was determined using a Qubit double-stranded DNA (dsDNA) broad-range (BR) assay kit (Invitrogen). .. Prior to SMRT sequencing, NCIMB 702410 genomic DNA underwent BluePippin size selection (Sage Science, Beverly, MA) to eliminate shorter fragments and to enrich sequencing of DNA fragments of > 10 kb.

    Electrophoresis:

    Article Title: Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood
    Article Snippet: DNA integrity and purity were assessed by agarose gel electrophoresis on 0.8% E-Gels® (Life Technologies). .. DNA mass was determined using Qubit® Broad Range DNA kit (Life Technologies).

    Article Title: SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)
    Article Snippet: Input genomic DNA (gDNA) concentration was measured on a Qubit Fluorometer dsDNA Broad Range (Life Technologies, cat. no. 32850). .. Input genomic DNA (gDNA) concentration was measured on a Qubit Fluorometer dsDNA Broad Range (Life Technologies, cat. no. 32850).

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 . .. Each sample was amplified in three independent 10 µl reactions to ensure sufficient PCR product for next-generation sequencing.

    RNA Extraction:

    Article Title: Transcriptome profiling of monocytes from XLA patients revealed the innate immune function dysregulation due to the BTK gene expression deficiency
    Article Snippet: Paragraph title: RNA extraction, library preparation and sequencing ... The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA).

    Article Title: Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia
    Article Snippet: Paragraph title: RNA extraction and quantitative PCR ... RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat # ) and Invitrogen Qubit 2.0 Fluorometer (Cat # ).

    Article Title: Evaluating the Performance of De Novo Assembly Methods for Venom-Gland Transcriptomics
    Article Snippet: Venom gland RNA extraction was accomplished with Trizol (Invitrogen, Carlsbad, CA, USA) extraction, where homogenized gland tissue was transferred to 500 μL of Trizol. .. The concentration of the RNA was determined with the Qubit Broad Range RNA assay (Thermo-Fisher Scientific, Waltham, MA, USA) and quality of the extraction was checked on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

    Selection:

    Article Title: Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940
    Article Snippet: The final library was validated on a Bioanalyzer instrument (Agilent) using a DNA7500 chip, and concentration was determined using a Qubit double-stranded DNA (dsDNA) broad-range (BR) assay kit (Invitrogen). .. Additional sequencing was performed by the Department of Energy’s Joint Genome Institute on both M. elsdenii NCIMB 702410 and ATCC 25940, using single-molecule real-time (SMRT) sequencing ( ) on the PacBio RS II platform (Pacific Biosciences, Menlo Park, CA) to produce longer reads.

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen). .. After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Agarose Gel Electrophoresis:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: Nucleic acid quality and quantity were assessed using a Nano-Drop 1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and agarose gel electrophoresis. .. DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA).

    Article Title: Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood
    Article Snippet: DNA integrity and purity were assessed by agarose gel electrophoresis on 0.8% E-Gels® (Life Technologies). .. DNA mass was determined using Qubit® Broad Range DNA kit (Life Technologies).

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen). .. After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Article Title: Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant, et al. Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant
    Article Snippet: 2.2 DNA quality was assessed via agarose gel electrophoresis on a 1% gel, and only nondegraded DNA (as judged by a tight high‐molecular weight band against a lambda standard) was selected for the library preparation stage. .. DNA was quantified using a Qubit Broad Range dsDNA Assay (ThermoFisher Scientific) according to the manufacturer's instructions and normalized to c. 7 ng/μl.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 . .. Each sample was amplified in three independent 10 µl reactions to ensure sufficient PCR product for next-generation sequencing.

    Article Title: CRISPR/Cas9 System as a Valuable Genome Editing Tool for Wine Yeasts with Application to Decrease Urea Production
    Article Snippet: Amplicons were separated on 1% (w/v) agarose gel prepared in TBE buffer (0.09 M Tris, 0.09 M Boric acid, 2 mM EDTA) with 0.05 μg/L ethidium bromide and bands were UV visualized (Geldoc 1000 System, Bio-Rad Laboratories, California). .. Bands were extracted from gel, eluted in 50 μL of mQ water using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and quantified by Qubit® dsDNA BR (Broad-Range) Assay Kits (Invitrogen).

    Ethanol Precipitation:

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: The digested DNA was then purified using phenol:chloroform extraction and ethanol precipitation in the presence of glycogen, before blunt-ending and phosphorylation. .. After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Article Title: Systematic biases in DNA copy number originate from isolation procedures
    Article Snippet: DNA quality and quantity of all isolations were measured using NanoDrop ND-1000 (Thermo scientific) and a Qubit Quant-iT™ dsDNA broad range assay (Invitrogen). .. DNA quality and quantity of all isolations were measured using NanoDrop ND-1000 (Thermo scientific) and a Qubit Quant-iT™ dsDNA broad range assay (Invitrogen).

    Next-Generation Sequencing:

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
    Article Snippet: Paragraph title: Next-Generation Sequencing of 5-mC ... After cleanup with AMPure XP beads (Product #A63880; Beckman Coulter), libraries were quantified using the Agilent TapeStation genomic DNA kit (Catalog #G2991AA; Agilent) and Qubit broad range dsDNA (Catalog #Q32850; Invitrogen).

    Article Title: Molecular Preadaptation to Antimony Resistance in Leishmania donovani on the Indian Subcontinent
    Article Snippet: Paragraph title: DNA preparation for NGS and data analysis. ... DNA isolation was done using QIAamp DNA blood minikit (Qiagen), and the DNA concentration was assessed with the Qubit DNA broad-range DNA quantification kit (Thermo Fisher).

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 . .. Replicate 10 µl reactions were performed for all eDNA and control samples, using optimized conditions (as above) and modified primers containing Illumina sequencing primer and adapter sequences.

    Article Title: Next-generation sequencing of acute myeloid leukemia identifies the significance of TP53, U2AF1, ASXL1, and TET2 mutations
    Article Snippet: Paragraph title: Next-Generation Sequencing ... 125 ng of total genomic DNA was used and quantified using the Qubit DNA BR assay (Life Technologies, Carlsbad, CA, USA).

    dsDNA Assay:

    Article Title: Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant, et al. Single‐nucleotide polymorphism discovery and panel characterization in the African forest elephant
    Article Snippet: 2.2 DNA quality was assessed via agarose gel electrophoresis on a 1% gel, and only nondegraded DNA (as judged by a tight high‐molecular weight band against a lambda standard) was selected for the library preparation stage. .. DNA was quantified using a Qubit Broad Range dsDNA Assay (ThermoFisher Scientific) according to the manufacturer's instructions and normalized to c. 7 ng/μl. .. A ddRAD library was constructed according to a modified protocol of the original Peterson et al. ( ) methodology.

    Spectrophotometry:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: Nucleic acid quality and quantity were assessed using a Nano-Drop 1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and agarose gel electrophoresis. .. DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA).

    Concentration Assay:

    Article Title: Distribution of somatic mutations of cancer-related genes according to microsatellite instability status in Korean gastric cancer
    Article Snippet: Nucleic acid quality and quantity were assessed using a Nano-Drop 1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and agarose gel electrophoresis. .. DNA concentration was determined using the Broad range Qubit DNA kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA). .. The study protocol was approved by the Institutional Review Board of The Catholic University of Korea, including written informed consent for clinical and molecular analyses.

    Article Title: Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940
    Article Snippet: A Nextera XT library (Illumina, San Diego, CA) was prepared from M. elsdenii NCIMB 702410 genomic DNA, as described in the manufacturer’s protocol. .. The final library was validated on a Bioanalyzer instrument (Agilent) using a DNA7500 chip, and concentration was determined using a Qubit double-stranded DNA (dsDNA) broad-range (BR) assay kit (Invitrogen). .. Paired-end sequencing (2 × 301 bp) was completed using the Illumina MiSeq platform.

    Article Title: SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)
    Article Snippet: The SMRTbell Express Template Prep Kit (PacBio, cat. no. 101‐357‐000) was used to produce the insert library. .. Input genomic DNA (gDNA) concentration was measured on a Qubit Fluorometer dsDNA Broad Range (Life Technologies, cat. no. 32850). .. Next, 10 μg of gDNA was mechanically sheared to an average size distribution of 40–50 kbp using a Megaruptor Device (Diagenode, cat. no. B06010001).

    Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
    Article Snippet: Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher). .. Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

    Article Title: Evaluating the Performance of De Novo Assembly Methods for Venom-Gland Transcriptomics
    Article Snippet: The addition of isopropyl alcohol was used to precipitate the RNA, which was then washed with 75% ethanol and dissolved in Ultrapure water (Invitrogen). .. The concentration of the RNA was determined with the Qubit Broad Range RNA assay (Thermo-Fisher Scientific, Waltham, MA, USA) and quality of the extraction was checked on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. Total RNA from venom glands, with equimolar pooling of right and left venom gland RNA from the snakes, was used for mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), followed by the preparation of cDNA libraries using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) and Multiplex Oligos for Illumina (New England Biolabs), according to the manufacturer’s instructions.

    Article Title: Molecular Preadaptation to Antimony Resistance in Leishmania donovani on the Indian Subcontinent
    Article Snippet: Parasites were pelleted after 5 weeks of culture with a given concentration of SbIII . .. DNA isolation was done using QIAamp DNA blood minikit (Qiagen), and the DNA concentration was assessed with the Qubit DNA broad-range DNA quantification kit (Thermo Fisher). .. Sequencing libraries were prepared with the TruSeq DNA library prep kit (Illumina) according to the manufacturer’s instructions.

    Article Title: A striking new species of leaf warbler from the Lesser Sundas as uncovered through morphology and genomics
    Article Snippet: We used the restriction enzymes EcoRI and MspI, and performed DNA fragment cleanup steps with Sera-Mag magnetic beads (Thermo-Scientific, Fremont, California). .. Final concentration and fragment size checks on the library were performed using a Qubit 2.0 Broad Range DNA assay (Invitrogen, California, USA) and a Fragment Analyser (Advanced Analytical Technologies, Iowa, USA), respectively. .. Sequencing was performed on a 150 bp paired-end Illumina HiSeq2500 run at the Singapore Center for Environmental Life Sciences Engineering.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Positive controls were generated using genomic DNA from four Sanger sequenced, vouchered harbour porpoise samples to test for PCR or sequencing error. .. DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 . .. Replicate 10 µl reactions were performed for all eDNA and control samples, using optimized conditions (as above) and modified primers containing Illumina sequencing primer and adapter sequences.

    Article Title: Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
    Article Snippet: Genomic DNA from five samples of each group was extracted using the Wizard Genomic DNA purification kit (Promega, A1120). .. DNA concentration was measured by the Qubit dsDNA Broad-Range Assay (Invitrogen, Q32850). .. Two DNA samples were randomly selected from each group and named as Control 1, Control 2, Vaccinated 1 and Vaccinated 2, respectively.

    DNA Purification:

    Article Title: Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
    Article Snippet: Genomic DNA from five samples of each group was extracted using the Wizard Genomic DNA purification kit (Promega, A1120). .. DNA concentration was measured by the Qubit dsDNA Broad-Range Assay (Invitrogen, Q32850).

    Gel Extraction:

    Article Title: CRISPR/Cas9 System as a Valuable Genome Editing Tool for Wine Yeasts with Application to Decrease Urea Production
    Article Snippet: Amplicons were separated on 1% (w/v) agarose gel prepared in TBE buffer (0.09 M Tris, 0.09 M Boric acid, 2 mM EDTA) with 0.05 μg/L ethidium bromide and bands were UV visualized (Geldoc 1000 System, Bio-Rad Laboratories, California). .. Bands were extracted from gel, eluted in 50 μL of mQ water using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and quantified by Qubit® dsDNA BR (Broad-Range) Assay Kits (Invitrogen). .. All ligation reactions were performed using Rapid DNA Ligation Kit (Thermo Fisher Scientific, MA, USA) according to operating instructions.

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    Thermo Fisher qubit dsdna broad range kit
    Qubit Dsdna Broad Range Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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