quantitect sybr green pcr master mix  (Qiagen)


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    Qiagen quantitect sybr green pcr master mix
    Quantitect Sybr Green Pcr Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect sybr green pcr master mix/product/Qiagen
    Average 95 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    quantitect sybr green pcr master mix - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: .. Ideal performance of the assay was achieved by the use of Quantitect SYBR Green PCR Master Mix (Qiagen) along with the optimized concentrations of primers and magnesium chloride as mentioned in Materials and methods Section 2.4. .. Internal control validation Specific internal control primers that target unique sequences of the bovine GAPDH mRNA were designed to validate all the reaction steps from RNA extraction to qPCR amplification/detection ( Table 2 ).

    Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
    Article Snippet: .. RT‐qPCR of microRNAs was performed using the QuantiTect SYBR Green PCR Master Mix with miScript Universal Primers and the specific miRNA primer sequences (Qiagen, Hilden, Germany). .. For mRNA expression, RT‐qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (KapaBiosystems, Inc., Wilmington, MA, USA).

    RNA Extraction:

    Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
    Article Snippet: Paragraph title: RNA extraction and gene expression analysis ... RT‐qPCR of microRNAs was performed using the QuantiTect SYBR Green PCR Master Mix with miScript Universal Primers and the specific miRNA primer sequences (Qiagen, Hilden, Germany).

    Selection:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: Criteria for selection of the proper measures were the intensity of fluorescence and the number of cycles required to develop a detectable signal (C T values). .. Ideal performance of the assay was achieved by the use of Quantitect SYBR Green PCR Master Mix (Qiagen) along with the optimized concentrations of primers and magnesium chloride as mentioned in Materials and methods Section 2.4.

    Fluorescence:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: Criteria for selection of the proper measures were the intensity of fluorescence and the number of cycles required to develop a detectable signal (C T values). .. Ideal performance of the assay was achieved by the use of Quantitect SYBR Green PCR Master Mix (Qiagen) along with the optimized concentrations of primers and magnesium chloride as mentioned in Materials and methods Section 2.4.

    Isolation:

    Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
    Article Snippet: For other studies, RNA was isolated using RNeasy® Mini Kit (Qiagen, Hilden, Germany), and 3 μg of RNA was reverse transcribed using oligo (dT) primers (Promega, Madison, WI, USA). .. RT‐qPCR of microRNAs was performed using the QuantiTect SYBR Green PCR Master Mix with miScript Universal Primers and the specific miRNA primer sequences (Qiagen, Hilden, Germany).

    Real-time Polymerase Chain Reaction:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: Paragraph title: Optimization of the SYBR Green I based real-time PCR assay ... Ideal performance of the assay was achieved by the use of Quantitect SYBR Green PCR Master Mix (Qiagen) along with the optimized concentrations of primers and magnesium chloride as mentioned in Materials and methods Section 2.4.

    Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
    Article Snippet: RT‐qPCR of microRNAs was performed using the QuantiTect SYBR Green PCR Master Mix with miScript Universal Primers and the specific miRNA primer sequences (Qiagen, Hilden, Germany). .. For mRNA expression, RT‐qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (KapaBiosystems, Inc., Wilmington, MA, USA).

    Concentration Assay:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: Such parameters included: magnesium chloride concentration (2.5e4 mM); concentration of the two sets of primers (0.2e1 mM); template volume (1e5 ml); and annealing temperature (55e60 C). .. Ideal performance of the assay was achieved by the use of Quantitect SYBR Green PCR Master Mix (Qiagen) along with the optimized concentrations of primers and magnesium chloride as mentioned in Materials and methods Section 2.4.

    Expressing:

    Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
    Article Snippet: Paragraph title: RNA extraction and gene expression analysis ... RT‐qPCR of microRNAs was performed using the QuantiTect SYBR Green PCR Master Mix with miScript Universal Primers and the specific miRNA primer sequences (Qiagen, Hilden, Germany).

    Polymerase Chain Reaction:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: .. Ideal performance of the assay was achieved by the use of Quantitect SYBR Green PCR Master Mix (Qiagen) along with the optimized concentrations of primers and magnesium chloride as mentioned in Materials and methods Section 2.4. .. Internal control validation Specific internal control primers that target unique sequences of the bovine GAPDH mRNA were designed to validate all the reaction steps from RNA extraction to qPCR amplification/detection ( Table 2 ).

    Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
    Article Snippet: .. RT‐qPCR of microRNAs was performed using the QuantiTect SYBR Green PCR Master Mix with miScript Universal Primers and the specific miRNA primer sequences (Qiagen, Hilden, Germany). .. For mRNA expression, RT‐qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (KapaBiosystems, Inc., Wilmington, MA, USA).

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    Qiagen quantitect sybr green pcr master mix
    The expression of eight miRNAs detected by <t>SYBR</t> Green real-time <t>PCR</t> in oyster haemocytes after bacteria challenge and heat stress, including cgi-miR-8 (A), cgi-miR-12 (B), cgi-miR-100 (C), cgi-miR-125 (D), cgi-miR-1984 (E), scaffold631_909 (F), scaffold42648_5080 (G) and scaffold1599_5643 (H). 5S gene was used as an internal control to calibrate the cDNA template for all the samples. Each values were shown as mean ± SD (N = 5), and bars with different letters were significantly different ( P
    Quantitect Sybr Green Pcr Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect sybr green pcr master mix/product/Qiagen
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    quantitect sybr green pcr master mix - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen quantitect sybr green rt pcr master mix
    Sensitivity and specificity of one-tube <t>qRT-PCR</t> for detection of Nipah virus RNA. DNA fragments obtained from the RT-PCR were visualized in ethidium bromide-stained agarose gel (a). Input Nipah virus RNA in equivalent log PFU is indicated above the lanes. RNA extracted from mock-infected Vero cells and the Nipah virus Armored RNA ® served as the negative (neg) and positive (pos) controls, respectively. Lane (M) consisted of DNA molecular mass marker. Amplification plot of the <t>SYBR</t> ® Green I dye-based qRT-PCR assay were obtained from tenfold serial diluted Nipah virus RNA (1 × 10 6 to 1 PFU) as indicated in (b). RNA extracted from mock-infected Vero cells was used as the negative control (NTC). The standard curve for the qRT-PCR (c) was generated using the same dilution series of Nipah virus RNA as the amplification plot. Correlation between log PFU/μL of infectious virus against total copy number of Nipah virus RNA (log RNA copy/μL) obtained from the qRT-PCR is shown in (d). Specificity of the assay was assessed and the difference in the melting temperature of the amplified DNA of Nipah virus (thick arrow) and Hendra virus (thin arrow) is indicated in the melting curve analysis (e).
    Quantitect Sybr Green Rt Pcr Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect sybr green rt pcr master mix/product/Qiagen
    Average 99 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    quantitect sybr green rt pcr master mix - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    The expression of eight miRNAs detected by SYBR Green real-time PCR in oyster haemocytes after bacteria challenge and heat stress, including cgi-miR-8 (A), cgi-miR-12 (B), cgi-miR-100 (C), cgi-miR-125 (D), cgi-miR-1984 (E), scaffold631_909 (F), scaffold42648_5080 (G) and scaffold1599_5643 (H). 5S gene was used as an internal control to calibrate the cDNA template for all the samples. Each values were shown as mean ± SD (N = 5), and bars with different letters were significantly different ( P

    Journal: PLoS ONE

    Article Title: The Identification and Characteristics of Immune-Related MicroRNAs in Haemocytes of Oyster Crassostrea gigas

    doi: 10.1371/journal.pone.0088397

    Figure Lengend Snippet: The expression of eight miRNAs detected by SYBR Green real-time PCR in oyster haemocytes after bacteria challenge and heat stress, including cgi-miR-8 (A), cgi-miR-12 (B), cgi-miR-100 (C), cgi-miR-125 (D), cgi-miR-1984 (E), scaffold631_909 (F), scaffold42648_5080 (G) and scaffold1599_5643 (H). 5S gene was used as an internal control to calibrate the cDNA template for all the samples. Each values were shown as mean ± SD (N = 5), and bars with different letters were significantly different ( P

    Article Snippet: The quantitative real-time PCR was carried out in a total volume of 25.0 µL, containing 12.5 µL of 2x QuantiTect SYBR Green PCR Master Mix (QIAGEN, miScript SYBR Green PCR Kit), 2.5 µL of diluted cDNA, 2.5 µL of each primers (10 mmol L−1 ), and 5.0 µL of RNase-free water.

    Techniques: Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Oxygen glucose deprivation disrupts carnitine homeostasis in rat hippocampal slice cultures. Rat hippocampal slice cultures were exposed to OGD in the presence or absence of L-carnitine (LCAR, 5 mM, 2 h prior to OGD). Slices were harvested 2 h after OGD and Protein extracts (50 µg) were subjected to Western blot analysis to determine effects on CPT1 (A), CPT2 (B), and CrAT (C) protein levels. A representative blot is shown in the inset of each panel. Two hours after OGD total RNA was also isolated and mRNA levels for CTP1 (D) and CTP2 (E) were determined by SYBR Green real-time RT-PCR analyses. Both protein and mRNA expression was normalized using β-actin. In addition, the effect of OGD, in the presence and absence of LCAR, on free carnitine levels (FC, F) and the acylcarnitine (AC): FC ratio (G) was determined. Data are presented as mean ± S.E from 3 independent experiments using 60 pooled slices per experiment. * = P

    Journal: PLoS ONE

    Article Title: Oxygen Glucose Deprivation in Rat Hippocampal Slice Cultures Results in Alterations in Carnitine Homeostasis and Mitochondrial Dysfunction

    doi: 10.1371/journal.pone.0040881

    Figure Lengend Snippet: Oxygen glucose deprivation disrupts carnitine homeostasis in rat hippocampal slice cultures. Rat hippocampal slice cultures were exposed to OGD in the presence or absence of L-carnitine (LCAR, 5 mM, 2 h prior to OGD). Slices were harvested 2 h after OGD and Protein extracts (50 µg) were subjected to Western blot analysis to determine effects on CPT1 (A), CPT2 (B), and CrAT (C) protein levels. A representative blot is shown in the inset of each panel. Two hours after OGD total RNA was also isolated and mRNA levels for CTP1 (D) and CTP2 (E) were determined by SYBR Green real-time RT-PCR analyses. Both protein and mRNA expression was normalized using β-actin. In addition, the effect of OGD, in the presence and absence of LCAR, on free carnitine levels (FC, F) and the acylcarnitine (AC): FC ratio (G) was determined. Data are presented as mean ± S.E from 3 independent experiments using 60 pooled slices per experiment. * = P

    Article Snippet: Quantitative real-time PCR was conducted on an Mx4000 (Stratagene, La Jolla, CA, USA), using 2 μl of RT product, 12.5 µl of QuantiTect SYBR Green PCR Master Mix (Qiagen), and primers (400 nM) in a total volume of 25 μl.

    Techniques: Western Blot, Isolation, SYBR Green Assay, Quantitative RT-PCR, Expressing

    Sensitivity and specificity of one-tube qRT-PCR for detection of Nipah virus RNA. DNA fragments obtained from the RT-PCR were visualized in ethidium bromide-stained agarose gel (a). Input Nipah virus RNA in equivalent log PFU is indicated above the lanes. RNA extracted from mock-infected Vero cells and the Nipah virus Armored RNA ® served as the negative (neg) and positive (pos) controls, respectively. Lane (M) consisted of DNA molecular mass marker. Amplification plot of the SYBR ® Green I dye-based qRT-PCR assay were obtained from tenfold serial diluted Nipah virus RNA (1 × 10 6 to 1 PFU) as indicated in (b). RNA extracted from mock-infected Vero cells was used as the negative control (NTC). The standard curve for the qRT-PCR (c) was generated using the same dilution series of Nipah virus RNA as the amplification plot. Correlation between log PFU/μL of infectious virus against total copy number of Nipah virus RNA (log RNA copy/μL) obtained from the qRT-PCR is shown in (d). Specificity of the assay was assessed and the difference in the melting temperature of the amplified DNA of Nipah virus (thick arrow) and Hendra virus (thin arrow) is indicated in the melting curve analysis (e).

    Journal: Virology Journal

    Article Title: Quantitative estimation of Nipah virus replication kinetics in vitro

    doi: 10.1186/1743-422X-3-47

    Figure Lengend Snippet: Sensitivity and specificity of one-tube qRT-PCR for detection of Nipah virus RNA. DNA fragments obtained from the RT-PCR were visualized in ethidium bromide-stained agarose gel (a). Input Nipah virus RNA in equivalent log PFU is indicated above the lanes. RNA extracted from mock-infected Vero cells and the Nipah virus Armored RNA ® served as the negative (neg) and positive (pos) controls, respectively. Lane (M) consisted of DNA molecular mass marker. Amplification plot of the SYBR ® Green I dye-based qRT-PCR assay were obtained from tenfold serial diluted Nipah virus RNA (1 × 10 6 to 1 PFU) as indicated in (b). RNA extracted from mock-infected Vero cells was used as the negative control (NTC). The standard curve for the qRT-PCR (c) was generated using the same dilution series of Nipah virus RNA as the amplification plot. Correlation between log PFU/μL of infectious virus against total copy number of Nipah virus RNA (log RNA copy/μL) obtained from the qRT-PCR is shown in (d). Specificity of the assay was assessed and the difference in the melting temperature of the amplified DNA of Nipah virus (thick arrow) and Hendra virus (thin arrow) is indicated in the melting curve analysis (e).

    Article Snippet: The reaction mixture consisted of 1× QuantiTect SYBR® Green RT-PCR Master Mix (Qiagen, Germany), 0.5 μL QuantiTect RT Mix, 0.6 pmol/μL of each primer, and 1 μL template RNA.

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Infection, Marker, Amplification, SYBR Green Assay, Negative Control, Generated

    Nipah virus replication in Vero cells. Vero cells were infected with Nipah virus at MOI of 0.2. At selected intervals, total RNA was isolated and the Nipah virus RNA levels were quantified using the SYBR ® Green I-based qRT-PCR assay in equivalent log PFU. A latent phase of at least eight hours followed by an exponential increase in the virus RNA level were noted for the intracellular Nipah virus RNA.

    Journal: Virology Journal

    Article Title: Quantitative estimation of Nipah virus replication kinetics in vitro

    doi: 10.1186/1743-422X-3-47

    Figure Lengend Snippet: Nipah virus replication in Vero cells. Vero cells were infected with Nipah virus at MOI of 0.2. At selected intervals, total RNA was isolated and the Nipah virus RNA levels were quantified using the SYBR ® Green I-based qRT-PCR assay in equivalent log PFU. A latent phase of at least eight hours followed by an exponential increase in the virus RNA level were noted for the intracellular Nipah virus RNA.

    Article Snippet: The reaction mixture consisted of 1× QuantiTect SYBR® Green RT-PCR Master Mix (Qiagen, Germany), 0.5 μL QuantiTect RT Mix, 0.6 pmol/μL of each primer, and 1 μL template RNA.

    Techniques: Infection, Isolation, SYBR Green Assay, Quantitative RT-PCR

    Confirmation of silencing in TaIPK1 : RNAi lines in T 4 seeds. (A) Free Pi content of control C306 and TaIPK1 :RNAi lines were estimated using colorimetric based assays. (B) Relative fold change of TaIPK1 expression in wheat transgenic lines. RNAi lines from three independent events were subjected to expression analysis at 14 DAA stage. The cDNA templates were prepared from 2 μg of DNase free RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. (C) Total phytic acid in mature wheat grains of transgenic lines (T 4 ). PA was measured in the mature seeds collected from the primary tiller of each line. # Indicates significant differences at p

    Journal: Frontiers in Plant Science

    Article Title: RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation

    doi: 10.3389/fpls.2018.00259

    Figure Lengend Snippet: Confirmation of silencing in TaIPK1 : RNAi lines in T 4 seeds. (A) Free Pi content of control C306 and TaIPK1 :RNAi lines were estimated using colorimetric based assays. (B) Relative fold change of TaIPK1 expression in wheat transgenic lines. RNAi lines from three independent events were subjected to expression analysis at 14 DAA stage. The cDNA templates were prepared from 2 μg of DNase free RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. (C) Total phytic acid in mature wheat grains of transgenic lines (T 4 ). PA was measured in the mature seeds collected from the primary tiller of each line. # Indicates significant differences at p

    Article Snippet: For the confirmation of gene silencing, SYBR Green (Quanti-Tect SYBR Green RT-PCR Master mix, QIAGEN) based reactions were performed on ABI PRISM 7500 Fast Real-Time Platform (Applied Biosystems).

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR, SYBR Green Assay

    Differential expression analysis of three homoeologs of TaIPK1 at two seed developmental stages (14 and 21 DAA). Transcript specific primers were designed for 2AL, 2BL, 2DL of TaIPK1 homoeologs based on genomic information available at IWGSC. gDNA free cDNA was prepared using 2 μg of RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. The indicated error bars represents the standard deviation from three independent replicates.

    Journal: Frontiers in Plant Science

    Article Title: RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation

    doi: 10.3389/fpls.2018.00259

    Figure Lengend Snippet: Differential expression analysis of three homoeologs of TaIPK1 at two seed developmental stages (14 and 21 DAA). Transcript specific primers were designed for 2AL, 2BL, 2DL of TaIPK1 homoeologs based on genomic information available at IWGSC. gDNA free cDNA was prepared using 2 μg of RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. The indicated error bars represents the standard deviation from three independent replicates.

    Article Snippet: For the confirmation of gene silencing, SYBR Green (Quanti-Tect SYBR Green RT-PCR Master mix, QIAGEN) based reactions were performed on ABI PRISM 7500 Fast Real-Time Platform (Applied Biosystems).

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay, Standard Deviation