quantitect sybr green pcr kit  (Qiagen)


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    Structured Review

    Qiagen quantitect sybr green pcr kit
    Quantitect Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect sybr green pcr kit/product/Qiagen
    Average 99 stars, based on 2392 article reviews
    Price from $9.99 to $1999.99
    quantitect sybr green pcr kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Produced:

    Article Title: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.
    Article Snippet: Afterwards, cDNA was produced using the SuperScript III First Strand Synthesis System (Invitrogen) with random hexamers. .. To determine the copy numbers CatB/CatL mRNA expression, quantitative PCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), 1 μl of cDNA as template and the following primers: Cathepsin B 5′-TACAGCCCGACCTACAAACA-3′, 5′-CCATGATGTCCTT CTCGCTA-3'; Cathepsin L 5′-GCAGGTCATGAGTCCTTCCT-3′, 5′-CTTT ACGTAGCCACCCATGC-3'; β-actin 5′-GGCTCCCAGCACAATGAAGA-3′, 5′-GGAGCCGCCGATCCA-3'.

    Real-time Polymerase Chain Reaction:

    Article Title: Respiratory viruses in laryngeal croup of young children.
    Article Snippet: .. 16 Viral DNA was amplified in a real-time PCR with Qiagen Quantitect SYBR Green PCR kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's instructions. .. After an initial DNA polymerase activation step for 15 minutes, 45 PCR cycles were performed.

    Article Title: Nonlinear response to cancer nanotherapy due to macrophage interactions revealed by mathematical modeling and evaluated in a murine model via CRISPR-modulated macrophage polarization.
    Article Snippet: .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes. .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes.

    Article Title: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.
    Article Snippet: .. To determine the copy numbers CatB/CatL mRNA expression, quantitative PCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), 1 μl of cDNA as template and the following primers: Cathepsin B 5′-TACAGCCCGACCTACAAACA-3′, 5′-CCATGATGTCCTT CTCGCTA-3'; Cathepsin L 5′-GCAGGTCATGAGTCCTTCCT-3′, 5′-CTTT ACGTAGCCACCCATGC-3'; β-actin 5′-GGCTCCCAGCACAATGAAGA-3′, 5′-GGAGCCGCCGATCCA-3'. .. As standard, serial dilutions of expression plasmids for CatB, CatL, and β-actin were subjected to PCR analysis.

    Article Title: Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.
    Article Snippet: .. As regards faecal shedding in group C, the cDNAs synthesised in the former study and stored at À80 C were used in the current study for quantitative PCR, which was performed as previously described (Battilani et al 2006) on the Corbett Research Rotor-Gene Real Time Amplification system (RG-3000, Corbett Research, Mortlake, NSW, Australia) using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the forward and reverse primers FCoV1128f and FCoV1229r (Gut et al 1999) . .. The number of copies of RNA from each sampling was compared to each other using the non-parametric tests mentioned above.

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: .. Real-time PCR conditions The SYBR Green I based real-time PCR assay was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using QuantiTect SYBR Green PCR Kit (Qiagen). .. The assay was carried out in a total volume of 25 ml reaction mixture prepared in triplicates in 96-well optical reaction plates or MicroAmp Ò optical tubes (Applied Biosystems).

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions.

    RNA Extraction:

    Article Title: Human β-defensin 2 is involved in CCR2-mediated Nod2 signal transduction, leading to activation of the innate immune response in macrophages.
    Article Snippet: Paragraph title: RNA extraction and quantitative real-time RT-PCR ... Gene expression levels were measured by quantitative real-time RT-PCR with the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and an ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using 50 ng of first-strand cDNA under the following conditions: 95°C for 5 min followed by 40 cycles at 95°C for 15 s, 55°C for 30 s, and 72°C for 30 s. The expression levels were normalized to that of β-actin (hACTB) using 7500 FAST software version 2.0.6 (Applied Biosystems).

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: Paragraph title: RNA extraction and reverse transcription-PCR. ... The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions.

    Amplification:

    Article Title: Respiratory viruses in laryngeal croup of young children.
    Article Snippet: .. 16 Viral DNA was amplified in a real-time PCR with Qiagen Quantitect SYBR Green PCR kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's instructions. .. After an initial DNA polymerase activation step for 15 minutes, 45 PCR cycles were performed.

    Article Title: Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.
    Article Snippet: .. As regards faecal shedding in group C, the cDNAs synthesised in the former study and stored at À80 C were used in the current study for quantitative PCR, which was performed as previously described (Battilani et al 2006) on the Corbett Research Rotor-Gene Real Time Amplification system (RG-3000, Corbett Research, Mortlake, NSW, Australia) using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the forward and reverse primers FCoV1128f and FCoV1229r (Gut et al 1999) . .. The number of copies of RNA from each sampling was compared to each other using the non-parametric tests mentioned above.

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: Real-time PCR conditions The SYBR Green I based real-time PCR assay was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using QuantiTect SYBR Green PCR Kit (Qiagen). .. The reaction conditions were first optimized by testing variable concentrations of BCoV and internal control QPCR primer sets; MgCl 2 concentrations; template volumes and primer annealing Following amplification, a melt curve analysis was performed to verify the specificity of the amplified products by their specific melting temperatures (Tm).

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. To quantify the fold changes in expression of each gene in kainate-injected animals compared with control, we first calculated the mean of ΔCt obtained in four PBS-injected animals (ΔCtmPBS ).

    Fluorescence:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: Real-time PCR conditions The SYBR Green I based real-time PCR assay was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using QuantiTect SYBR Green PCR Kit (Qiagen). .. Melting curve analysis consisted of a denaturation step at 95 C for 15 s, decreased to 60 C for 1 min, and followed by temperature increase to 95 C at a rate of 1% with continuous reading of fluorescence.

    Article Title: SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway.
    Article Snippet: Novel real-time RT-PCR protocol with SYBR Green based chemistry PCR reactions were set up with 2.5 l of cDNA in a 25 l reaction mix using the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and 200 nM each of primer BoNoV72F and BoNoV72R2. .. Fluorescence was measured at 55 • C. Finally a melting curve analysis was made by continuously measuring the fluorescence between 55 • C and 95 • C. Cycling was performed on a Stratagene MX3005P real-time Q-PCR system and the Ct-values (threshold cycle) were reported from cycle 5.

    Activation Assay:

    Article Title: Respiratory viruses in laryngeal croup of young children.
    Article Snippet: 16 Viral DNA was amplified in a real-time PCR with Qiagen Quantitect SYBR Green PCR kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's instructions. .. After an initial DNA polymerase activation step for 15 minutes, 45 PCR cycles were performed.

    Isolation:

    Article Title: Nonlinear response to cancer nanotherapy due to macrophage interactions revealed by mathematical modeling and evaluated in a murine model via CRISPR-modulated macrophage polarization.
    Article Snippet: Paragraph title: RNA isolation and qPCR analysis ... Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes.

    Random Hexamer Labeling:

    Article Title: SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway.
    Article Snippet: Novel real-time RT-PCR protocol with SYBR Green based chemistry cDNA synthesis was performed in a 20 l RT-reaction containing 4 l RT buffer (Invitrogen, Carlsbad, USA), 1 l 10 mM dNTP (Invitrogen, Foster City, USA), 1 l 0.1 M DTT (Invitrogen, Foster City, USA), 1 l 5 M Random hexamer primer (Applied Biosystems, Foster City, USA), 1 l RNaseOut (40 U/l) (Invitrogen, Foster City, USA), 1 l RT Superscript III (200 U/l) (Invitrogen, Foster City, USA) and 11 l template (RNA eluate). .. Novel real-time RT-PCR protocol with SYBR Green based chemistry PCR reactions were set up with 2.5 l of cDNA in a 25 l reaction mix using the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and 200 nM each of primer BoNoV72F and BoNoV72R2.

    Cell Culture:

    Article Title: Nonlinear response to cancer nanotherapy due to macrophage interactions revealed by mathematical modeling and evaluated in a murine model via CRISPR-modulated macrophage polarization.
    Article Snippet: Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes. .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes.

    Sampling:

    Article Title: Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.
    Article Snippet: Moreover, hypersialylation elongates the half-life of circulating proteins (Gregoriadis et al 2005) which may partly explain why the concentration of serum AGP did not show, at sampling 7, the same increase recorded at sampling 5 (both these samplings were followed by outbreaks of FIP). .. As regards faecal shedding in group C, the cDNAs synthesised in the former study and stored at À80 C were used in the current study for quantitative PCR, which was performed as previously described (Battilani et al 2006) on the Corbett Research Rotor-Gene Real Time Amplification system (RG-3000, Corbett Research, Mortlake, NSW, Australia) using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the forward and reverse primers FCoV1128f and FCoV1229r (Gut et al 1999) .

    SYBR Green Assay:

    Article Title: Respiratory viruses in laryngeal croup of young children.
    Article Snippet: .. 16 Viral DNA was amplified in a real-time PCR with Qiagen Quantitect SYBR Green PCR kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's instructions. .. After an initial DNA polymerase activation step for 15 minutes, 45 PCR cycles were performed.

    Article Title: Nonlinear response to cancer nanotherapy due to macrophage interactions revealed by mathematical modeling and evaluated in a murine model via CRISPR-modulated macrophage polarization.
    Article Snippet: .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes. .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes.

    Article Title: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.
    Article Snippet: .. To determine the copy numbers CatB/CatL mRNA expression, quantitative PCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), 1 μl of cDNA as template and the following primers: Cathepsin B 5′-TACAGCCCGACCTACAAACA-3′, 5′-CCATGATGTCCTT CTCGCTA-3'; Cathepsin L 5′-GCAGGTCATGAGTCCTTCCT-3′, 5′-CTTT ACGTAGCCACCCATGC-3'; β-actin 5′-GGCTCCCAGCACAATGAAGA-3′, 5′-GGAGCCGCCGATCCA-3'. .. As standard, serial dilutions of expression plasmids for CatB, CatL, and β-actin were subjected to PCR analysis.

    Article Title: Human β-defensin 2 is involved in CCR2-mediated Nod2 signal transduction, leading to activation of the innate immune response in macrophages.
    Article Snippet: .. Gene expression levels were measured by quantitative real-time RT-PCR with the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and an ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using 50 ng of first-strand cDNA under the following conditions: 95°C for 5 min followed by 40 cycles at 95°C for 15 s, 55°C for 30 s, and 72°C for 30 s. The expression levels were normalized to that of β-actin (hACTB) using 7500 FAST software version 2.0.6 (Applied Biosystems). ..

    Article Title: Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.
    Article Snippet: .. As regards faecal shedding in group C, the cDNAs synthesised in the former study and stored at À80 C were used in the current study for quantitative PCR, which was performed as previously described (Battilani et al 2006) on the Corbett Research Rotor-Gene Real Time Amplification system (RG-3000, Corbett Research, Mortlake, NSW, Australia) using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the forward and reverse primers FCoV1128f and FCoV1229r (Gut et al 1999) . .. The number of copies of RNA from each sampling was compared to each other using the non-parametric tests mentioned above.

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: .. Real-time PCR conditions The SYBR Green I based real-time PCR assay was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using QuantiTect SYBR Green PCR Kit (Qiagen). .. The assay was carried out in a total volume of 25 ml reaction mixture prepared in triplicates in 96-well optical reaction plates or MicroAmp Ò optical tubes (Applied Biosystems).

    Article Title: SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway.
    Article Snippet: .. Novel real-time RT-PCR protocol with SYBR Green based chemistry PCR reactions were set up with 2.5 l of cDNA in a 25 l reaction mix using the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and 200 nM each of primer BoNoV72F and BoNoV72R2. .. Novel real-time RT-PCR protocol with SYBR Green based chemistry A touch-down PCR was performed, with the following cycling conditions: 15 min at 95 • C, 2× (94 • C for 1 min, 60 • C for 45 s and 72 • C for 1 min), 2× (94 • C for 1 min, 58 • C for 45 s and 72 • C for 1 min), and 40× (94 • C for 40 s, 55 • C for 30 s and 72 • C for 1 min).

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: .. The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. To quantify the fold changes in expression of each gene in kainate-injected animals compared with control, we first calculated the mean of ΔCt obtained in four PBS-injected animals (ΔCtmPBS ).

    Concentration Assay:

    Article Title: Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.
    Article Snippet: Data regarding serum AGP concentration (a), in mg/ml and antibody titres (a) recorded in the different samplings (S1eS7) from cats included in the study (from Paltrinieri et al 2007b) and percentage of sialylation recorded in individual sera after staining with the lectins SNAI (S) and MAA (M) were not statistically correlated with each other or with serum concentration of AGP, suggesting that AGP production and sialylation are driven by independent mechanisms. .. As regards faecal shedding in group C, the cDNAs synthesised in the former study and stored at À80 C were used in the current study for quantitative PCR, which was performed as previously described (Battilani et al 2006) on the Corbett Research Rotor-Gene Real Time Amplification system (RG-3000, Corbett Research, Mortlake, NSW, Australia) using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the forward and reverse primers FCoV1128f and FCoV1229r (Gut et al 1999) .

    Generated:

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. To quantify the fold changes in expression of each gene in kainate-injected animals compared with control, we first calculated the mean of ΔCt obtained in four PBS-injected animals (ΔCtmPBS ).

    Polymerase Chain Reaction:

    Article Title: Respiratory viruses in laryngeal croup of young children.
    Article Snippet: .. 16 Viral DNA was amplified in a real-time PCR with Qiagen Quantitect SYBR Green PCR kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's instructions. .. After an initial DNA polymerase activation step for 15 minutes, 45 PCR cycles were performed.

    Article Title: Nonlinear response to cancer nanotherapy due to macrophage interactions revealed by mathematical modeling and evaluated in a murine model via CRISPR-modulated macrophage polarization.
    Article Snippet: .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes. .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes.

    Article Title: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.
    Article Snippet: .. To determine the copy numbers CatB/CatL mRNA expression, quantitative PCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), 1 μl of cDNA as template and the following primers: Cathepsin B 5′-TACAGCCCGACCTACAAACA-3′, 5′-CCATGATGTCCTT CTCGCTA-3'; Cathepsin L 5′-GCAGGTCATGAGTCCTTCCT-3′, 5′-CTTT ACGTAGCCACCCATGC-3'; β-actin 5′-GGCTCCCAGCACAATGAAGA-3′, 5′-GGAGCCGCCGATCCA-3'. .. As standard, serial dilutions of expression plasmids for CatB, CatL, and β-actin were subjected to PCR analysis.

    Article Title: Human β-defensin 2 is involved in CCR2-mediated Nod2 signal transduction, leading to activation of the innate immune response in macrophages.
    Article Snippet: .. Gene expression levels were measured by quantitative real-time RT-PCR with the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and an ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using 50 ng of first-strand cDNA under the following conditions: 95°C for 5 min followed by 40 cycles at 95°C for 15 s, 55°C for 30 s, and 72°C for 30 s. The expression levels were normalized to that of β-actin (hACTB) using 7500 FAST software version 2.0.6 (Applied Biosystems). ..

    Article Title: Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.
    Article Snippet: .. As regards faecal shedding in group C, the cDNAs synthesised in the former study and stored at À80 C were used in the current study for quantitative PCR, which was performed as previously described (Battilani et al 2006) on the Corbett Research Rotor-Gene Real Time Amplification system (RG-3000, Corbett Research, Mortlake, NSW, Australia) using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the forward and reverse primers FCoV1128f and FCoV1229r (Gut et al 1999) . .. The number of copies of RNA from each sampling was compared to each other using the non-parametric tests mentioned above.

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: .. Real-time PCR conditions The SYBR Green I based real-time PCR assay was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using QuantiTect SYBR Green PCR Kit (Qiagen). .. The assay was carried out in a total volume of 25 ml reaction mixture prepared in triplicates in 96-well optical reaction plates or MicroAmp Ò optical tubes (Applied Biosystems).

    Article Title: SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway.
    Article Snippet: .. Novel real-time RT-PCR protocol with SYBR Green based chemistry PCR reactions were set up with 2.5 l of cDNA in a 25 l reaction mix using the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and 200 nM each of primer BoNoV72F and BoNoV72R2. .. Novel real-time RT-PCR protocol with SYBR Green based chemistry A touch-down PCR was performed, with the following cycling conditions: 15 min at 95 • C, 2× (94 • C for 1 min, 60 • C for 45 s and 72 • C for 1 min), 2× (94 • C for 1 min, 58 • C for 45 s and 72 • C for 1 min), and 40× (94 • C for 40 s, 55 • C for 30 s and 72 • C for 1 min).

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: .. The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. To quantify the fold changes in expression of each gene in kainate-injected animals compared with control, we first calculated the mean of ΔCt obtained in four PBS-injected animals (ΔCtmPBS ).

    Quantitative RT-PCR:

    Article Title: Human β-defensin 2 is involved in CCR2-mediated Nod2 signal transduction, leading to activation of the innate immune response in macrophages.
    Article Snippet: .. Gene expression levels were measured by quantitative real-time RT-PCR with the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and an ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using 50 ng of first-strand cDNA under the following conditions: 95°C for 5 min followed by 40 cycles at 95°C for 15 s, 55°C for 30 s, and 72°C for 30 s. The expression levels were normalized to that of β-actin (hACTB) using 7500 FAST software version 2.0.6 (Applied Biosystems). ..

    Article Title: SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway.
    Article Snippet: .. Novel real-time RT-PCR protocol with SYBR Green based chemistry PCR reactions were set up with 2.5 l of cDNA in a 25 l reaction mix using the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and 200 nM each of primer BoNoV72F and BoNoV72R2. .. Novel real-time RT-PCR protocol with SYBR Green based chemistry A touch-down PCR was performed, with the following cycling conditions: 15 min at 95 • C, 2× (94 • C for 1 min, 60 • C for 45 s and 72 • C for 1 min), 2× (94 • C for 1 min, 58 • C for 45 s and 72 • C for 1 min), and 40× (94 • C for 40 s, 55 • C for 30 s and 72 • C for 1 min).

    Expressing:

    Article Title: Nonlinear response to cancer nanotherapy due to macrophage interactions revealed by mathematical modeling and evaluated in a murine model via CRISPR-modulated macrophage polarization.
    Article Snippet: Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes. .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes.

    Article Title: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.
    Article Snippet: .. To determine the copy numbers CatB/CatL mRNA expression, quantitative PCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), 1 μl of cDNA as template and the following primers: Cathepsin B 5′-TACAGCCCGACCTACAAACA-3′, 5′-CCATGATGTCCTT CTCGCTA-3'; Cathepsin L 5′-GCAGGTCATGAGTCCTTCCT-3′, 5′-CTTT ACGTAGCCACCCATGC-3'; β-actin 5′-GGCTCCCAGCACAATGAAGA-3′, 5′-GGAGCCGCCGATCCA-3'. .. As standard, serial dilutions of expression plasmids for CatB, CatL, and β-actin were subjected to PCR analysis.

    Article Title: Human β-defensin 2 is involved in CCR2-mediated Nod2 signal transduction, leading to activation of the innate immune response in macrophages.
    Article Snippet: .. Gene expression levels were measured by quantitative real-time RT-PCR with the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and an ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using 50 ng of first-strand cDNA under the following conditions: 95°C for 5 min followed by 40 cycles at 95°C for 15 s, 55°C for 30 s, and 72°C for 30 s. The expression levels were normalized to that of β-actin (hACTB) using 7500 FAST software version 2.0.6 (Applied Biosystems). ..

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. To quantify the fold changes in expression of each gene in kainate-injected animals compared with control, we first calculated the mean of ΔCt obtained in four PBS-injected animals (ΔCtmPBS ).

    CRISPR:

    Article Title: Nonlinear response to cancer nanotherapy due to macrophage interactions revealed by mathematical modeling and evaluated in a murine model via CRISPR-modulated macrophage polarization.
    Article Snippet: Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes. .. Tumor-associated macrophages (TAMs) have been shown to both aid and hinder tumor growth, with patient outcomes potentially hinging on the proportion of M1, pro-inflammatory/growth-inhibiting, to M2, growth-supporting, phenotypes.

    Sequencing:

    Article Title: Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.
    Article Snippet: .. Real-time PCR conditions The SYBR Green I based real-time PCR assay was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using QuantiTect SYBR Green PCR Kit (Qiagen). .. The assay was carried out in a total volume of 25 ml reaction mixture prepared in triplicates in 96-well optical reaction plates or MicroAmp Ò optical tubes (Applied Biosystems).

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. To quantify the fold changes in expression of each gene in kainate-injected animals compared with control, we first calculated the mean of ΔCt obtained in four PBS-injected animals (ΔCtmPBS ).

    Staining:

    Article Title: Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.
    Article Snippet: Data regarding serum AGP concentration (a), in mg/ml and antibody titres (a) recorded in the different samplings (S1eS7) from cats included in the study (from Paltrinieri et al 2007b) and percentage of sialylation recorded in individual sera after staining with the lectins SNAI (S) and MAA (M) were not statistically correlated with each other or with serum concentration of AGP, suggesting that AGP production and sialylation are driven by independent mechanisms. .. As regards faecal shedding in group C, the cDNAs synthesised in the former study and stored at À80 C were used in the current study for quantitative PCR, which was performed as previously described (Battilani et al 2006) on the Corbett Research Rotor-Gene Real Time Amplification system (RG-3000, Corbett Research, Mortlake, NSW, Australia) using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the forward and reverse primers FCoV1128f and FCoV1229r (Gut et al 1999) .

    Mouse Assay:

    Article Title: Status Epilepticus Induces a Particular Microglial Activation State Characterized by Enhanced Purinergic Signaling
    Article Snippet: The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions. .. The cDNA was quantified using the Quantitect SYBR Green PCR kit and the Quantitect Primer assay kit (Qiagen) according to the manufacturer's instructions.

    Software:

    Article Title: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.
    Article Snippet: To determine the copy numbers CatB/CatL mRNA expression, quantitative PCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), 1 μl of cDNA as template and the following primers: Cathepsin B 5′-TACAGCCCGACCTACAAACA-3′, 5′-CCATGATGTCCTT CTCGCTA-3'; Cathepsin L 5′-GCAGGTCATGAGTCCTTCCT-3′, 5′-CTTT ACGTAGCCACCCATGC-3'; β-actin 5′-GGCTCCCAGCACAATGAAGA-3′, 5′-GGAGCCGCCGATCCA-3'. .. Ct values were determined using the Rotor-Gene Q device along with the Rotor-Gene Q software (Qiagen) and used to calculate their respective copy numbers.

    Article Title: Human β-defensin 2 is involved in CCR2-mediated Nod2 signal transduction, leading to activation of the innate immune response in macrophages.
    Article Snippet: .. Gene expression levels were measured by quantitative real-time RT-PCR with the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany) and an ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using 50 ng of first-strand cDNA under the following conditions: 95°C for 5 min followed by 40 cycles at 95°C for 15 s, 55°C for 30 s, and 72°C for 30 s. The expression levels were normalized to that of β-actin (hACTB) using 7500 FAST software version 2.0.6 (Applied Biosystems). ..

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  • 99
    Qiagen quantitect sybr green rt pcr kit
    T m analysis of real-time <t>PCR</t> with <t>SYBR</t> Green as fluorescent dye. T m analysis allows the differentiation of specific PCR product (amplicon) from non-specific amplification products, such as primer-dimers. Samples: 1 to 7, patient samples (stool); 8, negative control (water); 9, positive control (NoV positive stool). Amplified products from NoV template RNA can be reliably distinguished from non-specific products by different melting points. The melting temperature for the positive samples was 79.0 +/- 0.5°C. Non-specific products melt at lower temperatures.
    Quantitect Sybr Green Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect sybr green rt pcr kit/product/Qiagen
    Average 99 stars, based on 512 article reviews
    Price from $9.99 to $1999.99
    quantitect sybr green rt pcr kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen quantitect sybr green pcr kit
    <t>qRT-PCR.</t> Reverse transcription with random hexamer primers generated cDNA using total RNA from the wild type (WT) and SC-E1. Signals from qRT-PCR using specific primers for flaA or flaB were quantified with <t>SYBR</t> green fluorescent dye. The data from one
    Quantitect Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect sybr green pcr kit/product/Qiagen
    Average 99 stars, based on 2392 article reviews
    Price from $9.99 to $1999.99
    quantitect sybr green pcr kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    T m analysis of real-time PCR with SYBR Green as fluorescent dye. T m analysis allows the differentiation of specific PCR product (amplicon) from non-specific amplification products, such as primer-dimers. Samples: 1 to 7, patient samples (stool); 8, negative control (water); 9, positive control (NoV positive stool). Amplified products from NoV template RNA can be reliably distinguished from non-specific products by different melting points. The melting temperature for the positive samples was 79.0 +/- 0.5°C. Non-specific products melt at lower temperatures.

    Journal: BMC Infectious Diseases

    Article Title: Fast detection of Noroviruses using a real-time PCR assay and automated sample preparation

    doi: 10.1186/1471-2334-4-15

    Figure Lengend Snippet: T m analysis of real-time PCR with SYBR Green as fluorescent dye. T m analysis allows the differentiation of specific PCR product (amplicon) from non-specific amplification products, such as primer-dimers. Samples: 1 to 7, patient samples (stool); 8, negative control (water); 9, positive control (NoV positive stool). Amplified products from NoV template RNA can be reliably distinguished from non-specific products by different melting points. The melting temperature for the positive samples was 79.0 +/- 0.5°C. Non-specific products melt at lower temperatures.

    Article Snippet: QuantiTect™ SYBR® Green RT-PCR kit was purchased from Qiagen, (Qiagen, Hilden, Germany).

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Polymerase Chain Reaction, Amplification, Negative Control, Positive Control

    qRT-PCR. Reverse transcription with random hexamer primers generated cDNA using total RNA from the wild type (WT) and SC-E1. Signals from qRT-PCR using specific primers for flaA or flaB were quantified with SYBR green fluorescent dye. The data from one

    Journal: Journal of Bacteriology

    Article Title: Borrelia burgdorferi Uniquely Regulates Its Motility Genes and Has an Intricate Flagellar Hook-Basal Body Structure ▿

    doi: 10.1128/JB.01421-07

    Figure Lengend Snippet: qRT-PCR. Reverse transcription with random hexamer primers generated cDNA using total RNA from the wild type (WT) and SC-E1. Signals from qRT-PCR using specific primers for flaA or flaB were quantified with SYBR green fluorescent dye. The data from one

    Article Snippet: Quantitative PCR was performed with a LightCycler (Roche) using a QuantiTect SYBR green PCR kit (Qiagen).

    Techniques: Quantitative RT-PCR, Random Hexamer Labeling, Generated, SYBR Green Assay

    Specificity of LAMP (A), (B) and real-time PCR for GCV detection. Panel ( A ) shows green fluorescence after addition of SYBR Green to each reaction mixture under UV light, panel ( B ) electrophoresis of LAMP products in 2% agarose gel stained with GelRed™ DNA staining solution while panel ( C ) real-time PCR specificity plot. A - reference DNA of goose circovirus strain P_1_03, B - DNA of goose hemorrhagic polyomavirus (GHPV) strain 2003, C - Muscovy duck parvovirus strain FM, D - goose parvovirus strain B38, E - Fowl adenovirus type-1 strain CELO.

    Journal: Virology Journal

    Article Title: Loop-mediated isothermal amplification for the detection of goose circovirus

    doi: 10.1186/1743-422X-9-110

    Figure Lengend Snippet: Specificity of LAMP (A), (B) and real-time PCR for GCV detection. Panel ( A ) shows green fluorescence after addition of SYBR Green to each reaction mixture under UV light, panel ( B ) electrophoresis of LAMP products in 2% agarose gel stained with GelRed™ DNA staining solution while panel ( C ) real-time PCR specificity plot. A - reference DNA of goose circovirus strain P_1_03, B - DNA of goose hemorrhagic polyomavirus (GHPV) strain 2003, C - Muscovy duck parvovirus strain FM, D - goose parvovirus strain B38, E - Fowl adenovirus type-1 strain CELO.

    Article Snippet: The reactions were set up on in 0.2 ml OptiAmp® optical tubes with caps (Applied Biosystems, Foster City, California, United States) using Quantitect SYBR Green PCR Kit (Qiagen, Hilden, Germany) with application of outer primers F3 and B3 designed for LAMP.

    Techniques: Real-time Polymerase Chain Reaction, Fluorescence, SYBR Green Assay, Electrophoresis, Agarose Gel Electrophoresis, Staining

    Sensitivity of LAMP (A), (B) and real-time PCR for GCV detection. Panel (A) shows green fluorescence after addition of SYBR Green to each reaction mixture under UV light, panel (B) electrophoresis of LAMP products in 2% agarose gel stained with GelRed™ DNA staining solution while panel (C) real-time PCR sensitivity plot.

    Journal: Virology Journal

    Article Title: Loop-mediated isothermal amplification for the detection of goose circovirus

    doi: 10.1186/1743-422X-9-110

    Figure Lengend Snippet: Sensitivity of LAMP (A), (B) and real-time PCR for GCV detection. Panel (A) shows green fluorescence after addition of SYBR Green to each reaction mixture under UV light, panel (B) electrophoresis of LAMP products in 2% agarose gel stained with GelRed™ DNA staining solution while panel (C) real-time PCR sensitivity plot.

    Article Snippet: The reactions were set up on in 0.2 ml OptiAmp® optical tubes with caps (Applied Biosystems, Foster City, California, United States) using Quantitect SYBR Green PCR Kit (Qiagen, Hilden, Germany) with application of outer primers F3 and B3 designed for LAMP.

    Techniques: Real-time Polymerase Chain Reaction, Fluorescence, SYBR Green Assay, Electrophoresis, Agarose Gel Electrophoresis, Staining

    Quantitative real-time polymerase chain reaction analysis of mRNA levels of apoptotic genes in human lung cancer (HepG2) cells treated with zinc oxide nanoparticles (ZnO NPs) at the concentration of 15 μg/mL for 24 hours. Quantitative real-time polymerase chain reaction was performed by QuantiTect SYBR Green PCR kit using an ABI PRISM 7900HT sequence detection system. The β-actin was used as the internal control to normalize the data. ZnO NP-induced alterations in mRNA levels are expressed in relative quantity compared with those for the respective unexposed control cells. ( A ) p53, ( B ) bax, ( C ) bcl-2, and ( D ) caspase-3. Data represented are mean ± standard deviation of three identical experiments made in triplicate. Note: *Statistically significant difference as compared with the controls ( P

    Journal: International Journal of Nanomedicine

    Article Title: Zinc oxide nanoparticles selectively induce apoptosis in human cancer cells through reactive oxygen species

    doi: 10.2147/IJN.S29129

    Figure Lengend Snippet: Quantitative real-time polymerase chain reaction analysis of mRNA levels of apoptotic genes in human lung cancer (HepG2) cells treated with zinc oxide nanoparticles (ZnO NPs) at the concentration of 15 μg/mL for 24 hours. Quantitative real-time polymerase chain reaction was performed by QuantiTect SYBR Green PCR kit using an ABI PRISM 7900HT sequence detection system. The β-actin was used as the internal control to normalize the data. ZnO NP-induced alterations in mRNA levels are expressed in relative quantity compared with those for the respective unexposed control cells. ( A ) p53, ( B ) bax, ( C ) bcl-2, and ( D ) caspase-3. Data represented are mean ± standard deviation of three identical experiments made in triplicate. Note: *Statistically significant difference as compared with the controls ( P

    Article Snippet: Quantitative real-time PCR was performed by QuantiTect SYBR Green PCR kit (Qiagen) using an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Real-time Polymerase Chain Reaction, Concentration Assay, SYBR Green Assay, Polymerase Chain Reaction, Sequencing, Standard Deviation