quantitect reverse transcription kit  (Qiagen)


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    Name:
    QuantiTect Rev. Transcription Kit
    Description:
    For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis. Kit contents: Qiagen QuantiTect Reverse Transcription Kit, 10 x 20L rxns, RNA Template Sample, Two-step, cDNA Production, Genomic DNA Digestion Reaction Type, For Fast cDNA Synthesis Enabling Sensitive Real-time Two-step RT-PCR for Gene Expression Analysis, Includes 10 x 20L rxns: 100L 7x gDNA Wipeout Buffer, 10L Quantiscript Reverse Transcriptase, 200L 5x Quantiscript RT Buffer, 50L RT Primer Mix, 1.9mL RNase-free Water. Benefits: cDNA synthesis and gDNA removal in only 20 minutes. High cDNA yields even from low-abundance transcripts. cDNA synthesis from 5' and 3' regions of transcripts. No need to design RNA-specific primers or prob
    Catalog Number:
    205310
    Price:
    None
    Category:
    QuantiTect Reverse Transcription Kit
    Score:
    85
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    Structured Review

    Qiagen quantitect reverse transcription kit
    QuantiTect Rev. Transcription Kit
    For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis. Kit contents: Qiagen QuantiTect Reverse Transcription Kit, 10 x 20L rxns, RNA Template Sample, Two-step, cDNA Production, Genomic DNA Digestion Reaction Type, For Fast cDNA Synthesis Enabling Sensitive Real-time Two-step RT-PCR for Gene Expression Analysis, Includes 10 x 20L rxns: 100L 7x gDNA Wipeout Buffer, 10L Quantiscript Reverse Transcriptase, 200L 5x Quantiscript RT Buffer, 50L RT Primer Mix, 1.9mL RNase-free Water. Benefits: cDNA synthesis and gDNA removal in only 20 minutes. High cDNA yields even from low-abundance transcripts. cDNA synthesis from 5' and 3' regions of transcripts. No need to design RNA-specific primers or prob
    https://www.bioz.com/result/quantitect reverse transcription kit/product/Qiagen
    Average 99 stars, based on 973 article reviews
    Price from $9.99 to $1999.99
    quantitect reverse transcription kit - by Bioz Stars, 2019-12
    99/100 stars

    Images

    1) Product Images from "FITC Conjugation Markedly Enhances Hepatic Clearance of N-Formyl Peptides"

    Article Title: FITC Conjugation Markedly Enhances Hepatic Clearance of N-Formyl Peptides

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0160602

    Expression of FPR1 mRNA and protein in liver and isolated liver cells. (A) Total RNA was isolated from pelleted cells and whole tissue. cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit. PCR was performed in a 25 μl reaction mixture containing 2 μl of cDNA of hHeps (1), hLSECs (2), human liver (3), mHeps (4), mLSECs (5), and mouse liver (6). The house keeping genes were β-actin and GAPDH. (B) Homogenized tissue specimens and cells were lysed in a RIPA lysis buffer with protease and phosphatase inhibitor cocktails. Equal amounts of protein were loaded into NuPAGE Novex 4–12% Bis-Tris gel, then blotted onto PVDF membrane and probed with antibodies against FPR1 and β-actin.
    Figure Legend Snippet: Expression of FPR1 mRNA and protein in liver and isolated liver cells. (A) Total RNA was isolated from pelleted cells and whole tissue. cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit. PCR was performed in a 25 μl reaction mixture containing 2 μl of cDNA of hHeps (1), hLSECs (2), human liver (3), mHeps (4), mLSECs (5), and mouse liver (6). The house keeping genes were β-actin and GAPDH. (B) Homogenized tissue specimens and cells were lysed in a RIPA lysis buffer with protease and phosphatase inhibitor cocktails. Equal amounts of protein were loaded into NuPAGE Novex 4–12% Bis-Tris gel, then blotted onto PVDF membrane and probed with antibodies against FPR1 and β-actin.

    Techniques Used: Expressing, Isolation, Synthesized, Polymerase Chain Reaction, Lysis

    2) Product Images from "Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties"

    Article Title: Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0145749

    RT-PCR of total RNA isolated from superior (A) and inferior (B) spikelets of O. sativa cv. Mahalaxmi panicles on 0, 3 and 6 days after anthesis (DAA or simply D) for analysis of genes overexpressed in the superior compared with inferior spikelets (I) and genes overexpressed in the inferior compared with superior spikelets (II). Total RNA was isolated using TRIzol (Life Technologies) and reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen). Each gene was amplified using gene-specific primers designed using Primer Blast. Actin and 18S rRNA were amplified for 30 and 25 cycles, respectively, as positive and loading controls. The other reactions were performed for 30 cycles, and the amplified products were separated on an agarose gel containing ETBR and visualized and photographed using a Gel Doc (Bio-Rad). The primer sequences are provided in S2 Table .
    Figure Legend Snippet: RT-PCR of total RNA isolated from superior (A) and inferior (B) spikelets of O. sativa cv. Mahalaxmi panicles on 0, 3 and 6 days after anthesis (DAA or simply D) for analysis of genes overexpressed in the superior compared with inferior spikelets (I) and genes overexpressed in the inferior compared with superior spikelets (II). Total RNA was isolated using TRIzol (Life Technologies) and reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen). Each gene was amplified using gene-specific primers designed using Primer Blast. Actin and 18S rRNA were amplified for 30 and 25 cycles, respectively, as positive and loading controls. The other reactions were performed for 30 cycles, and the amplified products were separated on an agarose gel containing ETBR and visualized and photographed using a Gel Doc (Bio-Rad). The primer sequences are provided in S2 Table .

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Isolation, Amplification, Agarose Gel Electrophoresis

    3) Product Images from "Subcutaneous administration of a neutralizing IL‐1β antibody prolongs limb allograft survival, et al. Subcutaneous administration of a neutralizing IL‐1β antibody prolongs limb allograft survival"

    Article Title: Subcutaneous administration of a neutralizing IL‐1β antibody prolongs limb allograft survival, et al. Subcutaneous administration of a neutralizing IL‐1β antibody prolongs limb allograft survival

    Journal: American Journal of Transplantation

    doi: 10.1111/ajt.14765

    RT q PCR analysis showing relative gene expression levels of IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, TNF ‐α, and IFN ‐γ in allograft skin (A – H) and muscle (I – P). For RT q PCR , a total volume of 15 μl per reaction was used containing 50 ng cDNA template, 1.5 μl QuantiTect Primer Assay (Qiagen), 7.5 μl QuantiTect SYBR green PCR kit (Qiagen), and 5 μl ddH2O. Primers for IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, IFN ‐γ, and TNF ‐α were obtained from Qiagen. Cycling conditions included a hot start activation (5 minutes, 95°C), 40 cycles of 10 seconds denaturation (95°C), and annealing and extension (30 seconds, 60°C). Amplicons were quantified with the comparative threshold cycle ( CT ) method; data acquisition was performed using the 7500 System SDS Software Version 2.0.5 (Applied Biosystems). Amplification specificity was checked using melting curve according to the manufacturer's instructions. All samples were measured in duplicate and respective results were averaged. A–P, In allograft skin (A–H) and muscle (I–P) no significant differences in relative gene expression of cytokines was observed between anti‐ IL ‐1β‐treatment groups 3 and 4 and group 2 controls. Expression levels of all markers were normalized to those of group 2
    Figure Legend Snippet: RT q PCR analysis showing relative gene expression levels of IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, TNF ‐α, and IFN ‐γ in allograft skin (A – H) and muscle (I – P). For RT q PCR , a total volume of 15 μl per reaction was used containing 50 ng cDNA template, 1.5 μl QuantiTect Primer Assay (Qiagen), 7.5 μl QuantiTect SYBR green PCR kit (Qiagen), and 5 μl ddH2O. Primers for IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, IFN ‐γ, and TNF ‐α were obtained from Qiagen. Cycling conditions included a hot start activation (5 minutes, 95°C), 40 cycles of 10 seconds denaturation (95°C), and annealing and extension (30 seconds, 60°C). Amplicons were quantified with the comparative threshold cycle ( CT ) method; data acquisition was performed using the 7500 System SDS Software Version 2.0.5 (Applied Biosystems). Amplification specificity was checked using melting curve according to the manufacturer's instructions. All samples were measured in duplicate and respective results were averaged. A–P, In allograft skin (A–H) and muscle (I–P) no significant differences in relative gene expression of cytokines was observed between anti‐ IL ‐1β‐treatment groups 3 and 4 and group 2 controls. Expression levels of all markers were normalized to those of group 2

    Techniques Used: Polymerase Chain Reaction, Expressing, SYBR Green Assay, Activation Assay, Software, Amplification

    4) Product Images from "Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties"

    Article Title: Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0145749

    RT-PCR of total RNA isolated from superior (A) and inferior (B) spikelets of O. sativa cv. Mahalaxmi panicles on 0, 3 and 6 days after anthesis (DAA or simply D) for analysis of genes overexpressed in the superior compared with inferior spikelets (I) and genes overexpressed in the inferior compared with superior spikelets (II). Total RNA was isolated using TRIzol (Life Technologies) and reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen). Each gene was amplified using gene-specific primers designed using Primer Blast. Actin and 18S rRNA were amplified for 30 and 25 cycles, respectively, as positive and loading controls. The other reactions were performed for 30 cycles, and the amplified products were separated on an agarose gel containing ETBR and visualized and photographed using a Gel Doc (Bio-Rad). The primer sequences are provided in S2 Table .
    Figure Legend Snippet: RT-PCR of total RNA isolated from superior (A) and inferior (B) spikelets of O. sativa cv. Mahalaxmi panicles on 0, 3 and 6 days after anthesis (DAA or simply D) for analysis of genes overexpressed in the superior compared with inferior spikelets (I) and genes overexpressed in the inferior compared with superior spikelets (II). Total RNA was isolated using TRIzol (Life Technologies) and reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen). Each gene was amplified using gene-specific primers designed using Primer Blast. Actin and 18S rRNA were amplified for 30 and 25 cycles, respectively, as positive and loading controls. The other reactions were performed for 30 cycles, and the amplified products were separated on an agarose gel containing ETBR and visualized and photographed using a Gel Doc (Bio-Rad). The primer sequences are provided in S2 Table .

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Isolation, Amplification, Agarose Gel Electrophoresis

    Related Articles

    Amplification:

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    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
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    DNA Synthesis:

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    Stable Transfection:

    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
    Article Snippet: Cells were exposed to 17AAG and Nutlin-3a, alone or in combination, and collected after 24 h. Total RNA was extracted using the QIAzol lysis reagent and the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). cDNA was generated from purified RNA using a QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer's instructions. cDNAs corresponding to mRNAs encoding MDM2, MDM4, p21, PUMA, BAX, and PIG3 were amplified by PCR using the primers listed in . qRT-PCR was performed in triplicate using SYBR Green Mix (Qiagen) on a 7900HT Fast Real-time PCR System (Applied Bioscience, Foster, CA, USA). .. Relative target mRNA levels were normalized to GAPDH expression as internal efficiency control.

    Synthesized:

    Article Title: Ribosomal Proteins RPL37, RPS15 and RPS20 Regulate the Mdm2-p53-MdmX Network
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    Article Title: Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore
    Article Snippet: The quality of RNA was assessed using the NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, USA). .. The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions. .. 0.5 μ L of cDNA was used in each PCR reaction.

    Quantitative RT-PCR:

    Article Title: Ribosomal Proteins RPL37, RPS15 and RPS20 Regulate the Mdm2-p53-MdmX Network
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    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
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    Article Title: DEspR Roles in Tumor Vasculo-Angiogenesis, Invasiveness, CSC-Survival and Anoikis Resistance: A 'Common Receptor Coordinator' Paradigm
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    SYBR Green Assay:

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    Article Title: Identification of a Novel Gene Signature of ES Cells Self-Renewal Fluctuation through System-Wide Analysis
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    Incubation:

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    Expressing:

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    Article Title: Establishment of a Novel Murine Model of Ischemic Cardiomyopathy with Multiple Diffuse Coronary Lesions
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    Article Title: The Effect of Gestational Age on Angiogenic Gene Expression in the Rat Placenta
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    Article Title: Genotype-dependency of butyrate efficacy in children with congenital chloride diarrhea
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    Article Title: Altered Dynamics in the Circadian Oscillation of Clock Genes in Dermal Fibroblasts of Patients Suffering from Idiopathic Hypersomnia
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    Article Title: Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens
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    Electroporation:

    Article Title: Neurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte-Neuron Communication
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    Transfection:

    Article Title: Ribosomal Proteins RPL37, RPS15 and RPS20 Regulate the Mdm2-p53-MdmX Network
    Article Snippet: U2OS cells were transfected with Myc-RP as indicated. .. Total RNA was extracted from transfected cell pellets using the RNeasy Mini Kit (Qiagen), and cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen). .. Expression of each gene was determined in triplicate using SYBR Green (Applied Biosystems) on a StepOnePlus Real-Time PCR machine (Applied Biosystems).

    Article Title: Neurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte-Neuron Communication
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    Concentration Assay:

    Article Title: DEspR Roles in Tumor Vasculo-Angiogenesis, Invasiveness, CSC-Survival and Anoikis Resistance: A 'Common Receptor Coordinator' Paradigm
    Article Snippet: Total cytoplasmic RNA concentration was determined by absorbance at 260 nm. .. After removal and documentation of no residual DNA contamination, cDNA synthesis was performed with the QuantiTect Reverse Transcription Kit (Qiagen, MD) using 400 ng of total cytoplasmic RNA, a DEspR specific primer (Reverse R188 bp : 5′-TGGACCAGAGAAATTGCTTG-3′ , ) and a Cyclophilin specific primer (Reverse: 5′-GAAGTCACCACCCTGACA-3′ ).

    Generated:

    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
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    Polymerase Chain Reaction:

    Article Title: Efficient and Simple Production of Insulin-Producing Cells from Embryonal Carcinoma Stem Cells Using Mouse Neonate Pancreas Extract, As a Natural Inducer
    Article Snippet: Quantiscript reverse transcriptase (Qiagene -QuantiTect Rev_Transcription Kit, 205311) was used for synthesis of cDNA with oligo-dT and random primers, according to the manufacturer's protocol. .. Real-time RT-PCRs of undifferentiated stem cell markers, Oct3/4 (POU Class 5 Homeobox 1), Sox-2 [SRY (Sex Determining Region Y)-Box 2] and Nanog (Nanog Homeobox) as well as pancreatic β cell genes, PDX-1 (pancreatic and duodenal homeobox 1), INS1 (insulin1), INS2 (insulin2), EP300 (E1A binding protein p300), CREB1 (cAMP responsive element binding protein 1) and β-2M (β2 microglobulin) cDNAs were performed by using specific primers ( ) and SYBR Premix Ex Taq (TaKaRa, RR081Q).

    Article Title: Neurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte-Neuron Communication
    Article Snippet: Transfection of pOL with NR1 siRNA (Dharmacon L-045931-01) was carried out using Lipofectamine RNAiMAX (Invitrogen). .. Exosomal RNA was isolated from 100,000× g pellets using miRNA high affinity kit (Roche). cDNA was synthesized using the Quantitect reverse transcription kit (Qiagen), and Cre and Pgk1 were amplified by PCR. .. Total brains derived from E10, E14, P0, P7, and adult C57Bl/6N mice were homogenized using TissueRuptor (Qiagen).

    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
    Article Snippet: Cycloheximide chase assays were performed by exposing cancer cells to 100 μ g/ml cycloheximide (Sigma-Aldrich) for 1, 2, 3, or 4 h and then lysing the cells for immunoblot analysis. .. Cells were exposed to 17AAG and Nutlin-3a, alone or in combination, and collected after 24 h. Total RNA was extracted using the QIAzol lysis reagent and the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). cDNA was generated from purified RNA using a QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer's instructions. cDNAs corresponding to mRNAs encoding MDM2, MDM4, p21, PUMA, BAX, and PIG3 were amplified by PCR using the primers listed in . qRT-PCR was performed in triplicate using SYBR Green Mix (Qiagen) on a 7900HT Fast Real-time PCR System (Applied Bioscience, Foster, CA, USA). .. Relative target mRNA levels were normalized to GAPDH expression as internal efficiency control.

    Article Title: Src tyrosine kinase signaling antagonizes nuclear localization of FOXO and inhibits its transcription factor activity
    Article Snippet: Paragraph title: Realtime PCR ... For quantitative real-time PCR, 500 ng of RNA were used for cDNA synthesis with a QuantiTect Reverse Transcription Kit (Quiagen).

    Article Title: Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer: Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers
    Article Snippet: Reverse transcription was performed employing the QuantiTect Reverse Transcription Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions. .. Following this step, real time qRT-PCR assays were performed on a Roche LightCycler 2.0 detection system (Roche Diagnostics GmbH, Mannheim, Germany) in 20 μ L volumes in glass capillaries, employing the QuantiTect Probe RT-PCR Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions.

    Article Title: Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes
    Article Snippet: For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions. .. For real-time PCR, reactions were performed in 10 μl volumes containing 1 μl cDNA (diluted 1:2), 5 μl KAPA SYBR® FAST Universal 2× qPCR Master Mix (KAPA Biosystems) containing SYBER-green dye, 0.2 μl ROX reference dye and 1 μl optimised primer pairs (listed in ).

    Article Title: Gene dosage-dependent rescue of HSP neurite defects in SPG4 patients' neurons
    Article Snippet: Paragraph title: PCR and qRT–PCR ... One microgram of RNA was reverse transcribed using the QuantiTect Reverse Transcription kit (Qiagen).

    Binding Assay:

    Article Title: Efficient and Simple Production of Insulin-Producing Cells from Embryonal Carcinoma Stem Cells Using Mouse Neonate Pancreas Extract, As a Natural Inducer
    Article Snippet: Quantiscript reverse transcriptase (Qiagene -QuantiTect Rev_Transcription Kit, 205311) was used for synthesis of cDNA with oligo-dT and random primers, according to the manufacturer's protocol. .. RT-PCR assays were performed using the Qiagen apparatus (Qiagen, Rotor-Gene, California, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes
    Article Snippet: RNA integrity was confirmed by gel electrophoresis. .. For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions.

    Fluorescence:

    Article Title: Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes
    Article Snippet: For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions. .. For real-time PCR, reactions were performed in 10 μl volumes containing 1 μl cDNA (diluted 1:2), 5 μl KAPA SYBR® FAST Universal 2× qPCR Master Mix (KAPA Biosystems) containing SYBER-green dye, 0.2 μl ROX reference dye and 1 μl optimised primer pairs (listed in ).

    Isolation:

    Article Title: Neurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte-Neuron Communication
    Article Snippet: Transfection of pOL with NR1 siRNA (Dharmacon L-045931-01) was carried out using Lipofectamine RNAiMAX (Invitrogen). .. Exosomal RNA was isolated from 100,000× g pellets using miRNA high affinity kit (Roche). cDNA was synthesized using the Quantitect reverse transcription kit (Qiagen), and Cre and Pgk1 were amplified by PCR. .. Total brains derived from E10, E14, P0, P7, and adult C57Bl/6N mice were homogenized using TissueRuptor (Qiagen).

    Article Title: Altered Dynamics in the Circadian Oscillation of Clock Genes in Dermal Fibroblasts of Patients Suffering from Idiopathic Hypersomnia
    Article Snippet: The isolation of the total RNA at the indicated times was performed using TRIzol® Reagent (invitrogen) followed by the QIAGEN RNeasy Mini Kit (Qiagen, Hilden, Germany). .. Applying the QuantiTect® Reverse Transcription Kit (Qiagen, Hilden, Germany) the total RNA was reverse transcribed into cDNA.

    Article Title: Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes
    Article Snippet: Total RNA was isolated from individual wells immediately after loading using an RNeasy Kit (Qiagen) according to the manufacturer's protocol. .. For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Gene dosage-dependent rescue of HSP neurite defects in SPG4 patients' neurons
    Article Snippet: Isolated RNA was quality controlled by visualization in ethidiumbromide containing TAE-agarose gels under UV light. .. One microgram of RNA was reverse transcribed using the QuantiTect Reverse Transcription kit (Qiagen).

    Article Title: Identification of a Novel Gene Signature of ES Cells Self-Renewal Fluctuation through System-Wide Analysis
    Article Snippet: Primer sets with suboptimal dissociation curves or amplification efficiencies outside of the 85–115% range were discarded. .. One microgram of total RNA, isolated from cells by TRIzol (Invitrogen), was reverse-transcribed by Quantitec reverse transcription kit (Qiagen) according to the manufacturer's instructions. qPCR analyses were performed using 7.5 ng cDNA per well in duplicate with the SYBR green master mix (Applied Biosystems) according to the manufacturer's instructions. .. Reactions were run on 7900HT system (Applied Biosystems).

    Size-exclusion Chromatography:

    Article Title: Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer: Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers
    Article Snippet: Reverse transcription was performed employing the QuantiTect Reverse Transcription Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions. .. Following this step, real time qRT-PCR assays were performed on a Roche LightCycler 2.0 detection system (Roche Diagnostics GmbH, Mannheim, Germany) in 20 μ L volumes in glass capillaries, employing the QuantiTect Probe RT-PCR Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions.

    Purification:

    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
    Article Snippet: Cycloheximide chase assays were performed by exposing cancer cells to 100 μ g/ml cycloheximide (Sigma-Aldrich) for 1, 2, 3, or 4 h and then lysing the cells for immunoblot analysis. .. Cells were exposed to 17AAG and Nutlin-3a, alone or in combination, and collected after 24 h. Total RNA was extracted using the QIAzol lysis reagent and the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). cDNA was generated from purified RNA using a QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer's instructions. cDNAs corresponding to mRNAs encoding MDM2, MDM4, p21, PUMA, BAX, and PIG3 were amplified by PCR using the primers listed in . qRT-PCR was performed in triplicate using SYBR Green Mix (Qiagen) on a 7900HT Fast Real-time PCR System (Applied Bioscience, Foster, CA, USA). .. Relative target mRNA levels were normalized to GAPDH expression as internal efficiency control.

    Article Title: Omega-3 Fatty Acid Enriched Chevon (Goat Meat) Lowers Plasma Cholesterol Levels and Alters Gene Expressions in Rats
    Article Snippet: Total RNA purity was determined by the 260/280 nm ratio of absorbance readings using NanoDrop ND-1000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Purified total RNA (1 µg) was reverse transcribed using a Quantitect reverse transcription kit (Qiagen, Hilden, Germany) in accordance with the manufacturer's recommended procedure. .. Real-time PCR was performed with the Bio-Rad CFX96 Touch (Bio-Rad Laboratories, Hercules, CA, USA) using optical grade plates using Quantifast SYBR green PCR kit (Cat. number 204054, Qiagen, Hilden, Germany).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Neurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte-Neuron Communication
    Article Snippet: Paragraph title: Transfections, RNAi, RT-PCR, and qPCR ... Exosomal RNA was isolated from 100,000× g pellets using miRNA high affinity kit (Roche). cDNA was synthesized using the Quantitect reverse transcription kit (Qiagen), and Cre and Pgk1 were amplified by PCR.

    Article Title: Establishment of a Novel Murine Model of Ischemic Cardiomyopathy with Multiple Diffuse Coronary Lesions
    Article Snippet: Paragraph title: Real-time reverse transcription polymerase chain reaction ... RNA was DNase-treated using SuperScript VILO and reverse-transcribed using the QuantiTect Reverse Transcription Kit (QIAGEN, Hilden, Germany).

    Article Title: Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore
    Article Snippet: Paragraph title: RNA extraction and reverse transcription (RT-PCR) ... The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions.

    Article Title: Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer: Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers
    Article Snippet: Reverse transcription was performed employing the QuantiTect Reverse Transcription Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions. .. Reverse transcription was performed employing the QuantiTect Reverse Transcription Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions.

    Software:

    Article Title: Ribosomal Proteins RPL37, RPS15 and RPS20 Regulate the Mdm2-p53-MdmX Network
    Article Snippet: Total RNA was extracted from transfected cell pellets using the RNeasy Mini Kit (Qiagen), and cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen). .. Total RNA was extracted from transfected cell pellets using the RNeasy Mini Kit (Qiagen), and cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen).

    Article Title: DEspR Roles in Tumor Vasculo-Angiogenesis, Invasiveness, CSC-Survival and Anoikis Resistance: A 'Common Receptor Coordinator' Paradigm
    Article Snippet: After removal and documentation of no residual DNA contamination, cDNA synthesis was performed with the QuantiTect Reverse Transcription Kit (Qiagen, MD) using 400 ng of total cytoplasmic RNA, a DEspR specific primer (Reverse R188 bp : 5′-TGGACCAGAGAAATTGCTTG-3′ , ) and a Cyclophilin specific primer (Reverse: 5′-GAAGTCACCACCCTGACA-3′ ). .. Amplification was performed with the QantiTect SYBR Green PCR Kit (Qiagen, MD) using the StepOnePlus PCR System (Applied Biosciences, CA) and the following two primer sets: for DEspR (Forward F1: 5′-GGGGTTCTATCACTTGCATC-3′ , ; Reverse R188 bp : 5′-TGGACCAGAGAAATTGCTTG-3′ , 88 bp amplicon, ) and for endogenous control, Cyclophilin A (Forward: 5′-GCGTCTCCTTTGAGCTGTT-3′ ; Reverse: 5′-GAAGTCACCACCCTGACA-3′ , 145 bp amplicon). qRT-PCR and cycling parameters were as follow: cDNA synthesis for 15 min at 42°C, thermal inactivation of reverse transcriptase for 3 min at 95°C and 40 cycles of PCR (melting for 15 sec at 94°C, annealing 30 sec at 55°C, synthesis for 30 sec at 72°C).

    Real-time Polymerase Chain Reaction:

    Article Title: Neurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte-Neuron Communication
    Article Snippet: Paragraph title: Transfections, RNAi, RT-PCR, and qPCR ... Exosomal RNA was isolated from 100,000× g pellets using miRNA high affinity kit (Roche). cDNA was synthesized using the Quantitect reverse transcription kit (Qiagen), and Cre and Pgk1 were amplified by PCR.

    Article Title: Genetic and epigenetic analysis of putative breast cancer stem cell models
    Article Snippet: RNA was quantified and assessed for purity by UV spectrophotometry. .. One microgram of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer’s instructions. qPCR was performed using LightCycler 480 (Roche). .. Reactions were performed in a total volume of 20 μl, comprising 1x SYBR Green I Master Mix (Roche), 20 ng cDNA and 25 μM of each primer (final concentration).

    Article Title: The Effect of Gestational Age on Angiogenic Gene Expression in the Rat Placenta
    Article Snippet: Paragraph title: Quantitative Real-Time PCR Validation Experiments ... RNA was reverse transcribed into complentary DNA strands, using the Quantitect Reverse transcription kit (Qiagen, VIC, Australia) following manufacturers instructions.

    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
    Article Snippet: Cycloheximide chase assays were performed by exposing cancer cells to 100 μ g/ml cycloheximide (Sigma-Aldrich) for 1, 2, 3, or 4 h and then lysing the cells for immunoblot analysis. .. Cells were exposed to 17AAG and Nutlin-3a, alone or in combination, and collected after 24 h. Total RNA was extracted using the QIAzol lysis reagent and the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). cDNA was generated from purified RNA using a QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer's instructions. cDNAs corresponding to mRNAs encoding MDM2, MDM4, p21, PUMA, BAX, and PIG3 were amplified by PCR using the primers listed in . qRT-PCR was performed in triplicate using SYBR Green Mix (Qiagen) on a 7900HT Fast Real-time PCR System (Applied Bioscience, Foster, CA, USA). .. Relative target mRNA levels were normalized to GAPDH expression as internal efficiency control.

    Article Title: Genotype-dependency of butyrate efficacy in children with congenital chloride diarrhea
    Article Snippet: Total RNA amount was quantified with Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, UK). .. One microgram of total RNA was retro-transcribed with Quantitect Reverse Transcription kit (Qiagen, Germany), cDNA was diluted for downstream applications like quantitative real-time PCR analysis. .. Expression levels of either SLC26A3 and SLC26A6 from treated and untreated cells were measured by semi-quantitative real-time PCR on LightCycler 480 Real-Time PCR System (Roche, Germany) with Taqman probe chemistry (experiments were performed in replicate using also SYbr Green chemistry).

    Article Title: Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens
    Article Snippet: Paragraph title: Nucleic acid extraction and relative quantitative real time PCR ... First strand cDNA was transcribed using the QuantiTect Reverse Transcription kit (Qiagen) according to the manufacturer’s protocols.

    Article Title: DEspR Roles in Tumor Vasculo-Angiogenesis, Invasiveness, CSC-Survival and Anoikis Resistance: A 'Common Receptor Coordinator' Paradigm
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) ... After removal and documentation of no residual DNA contamination, cDNA synthesis was performed with the QuantiTect Reverse Transcription Kit (Qiagen, MD) using 400 ng of total cytoplasmic RNA, a DEspR specific primer (Reverse R188 bp : 5′-TGGACCAGAGAAATTGCTTG-3′ , ) and a Cyclophilin specific primer (Reverse: 5′-GAAGTCACCACCCTGACA-3′ ).

    Article Title: Src tyrosine kinase signaling antagonizes nuclear localization of FOXO and inhibits its transcription factor activity
    Article Snippet: Extracted RNA was diluted in DEPC-treated water. .. For quantitative real-time PCR, 500 ng of RNA were used for cDNA synthesis with a QuantiTect Reverse Transcription Kit (Quiagen). .. A -RT reaction (without reverse transcriptase) with Actin5C primers was performed to control presence of genomic DNA in the RNA sample.

    Article Title: Technical Variations in Low-Input RNA-seq Methodologies
    Article Snippet: Paragraph title: Reverse transcription and quantitative RT-PCR (qPCR) ... About 10 μg of total RNA was treated for DNA removal and converted into first strand cDNA using Quantitect Reverse Transcription kit (Qiagen).

    Article Title: Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes
    Article Snippet: Paragraph title: RNA extraction, cDNA synthesis and real-time PCR ... For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Identification of a Novel Gene Signature of ES Cells Self-Renewal Fluctuation through System-Wide Analysis
    Article Snippet: Primer sets with suboptimal dissociation curves or amplification efficiencies outside of the 85–115% range were discarded. .. One microgram of total RNA, isolated from cells by TRIzol (Invitrogen), was reverse-transcribed by Quantitec reverse transcription kit (Qiagen) according to the manufacturer's instructions. qPCR analyses were performed using 7.5 ng cDNA per well in duplicate with the SYBR green master mix (Applied Biosystems) according to the manufacturer's instructions. .. Reactions were run on 7900HT system (Applied Biosystems).

    RNA Extraction:

    Article Title: Plasmacytoid Dendritic Cell Dynamics Tune Interferon-Alfa Production in SIV-Infected Cynomolgus Macaques
    Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... The QuantiTect Rev-Transcription kit (Qiagen) was used to produce cDNA.

    Article Title: Efficient and Simple Production of Insulin-Producing Cells from Embryonal Carcinoma Stem Cells Using Mouse Neonate Pancreas Extract, As a Natural Inducer
    Article Snippet: Paragraph title: RNA extraction and real-time RT-polymerase chain reaction ... Quantiscript reverse transcriptase (Qiagene -QuantiTect Rev_Transcription Kit, 205311) was used for synthesis of cDNA with oligo-dT and random primers, according to the manufacturer's protocol.

    Article Title: Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore
    Article Snippet: Paragraph title: RNA extraction and reverse transcription (RT-PCR) ... The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions.

    Article Title: Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes
    Article Snippet: Paragraph title: RNA extraction, cDNA synthesis and real-time PCR ... For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions.

    RNA Expression:

    Article Title: Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens
    Article Snippet: First strand cDNA was transcribed using the QuantiTect Reverse Transcription kit (Qiagen) according to the manufacturer’s protocols. .. The delta-delta Ct method was used to calculate relative gene expression levels between two samples.

    Agarose Gel Electrophoresis:

    Article Title: Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore
    Article Snippet: The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions. .. The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions.

    Electrophoresis:

    Article Title: Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore
    Article Snippet: The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions. .. The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions.

    Homogenization:

    Article Title: Plasmacytoid Dendritic Cell Dynamics Tune Interferon-Alfa Production in SIV-Infected Cynomolgus Macaques
    Article Snippet: Tissue lysates were passed through a QiaShredder (Qiagen) for homogenization, and total RNA was extracted using RNeasy MiniKits (Qiagen) according to the manufacturer's recommendations. .. The QuantiTect Rev-Transcription kit (Qiagen) was used to produce cDNA.

    Ethanol Precipitation:

    Article Title: DEspR Roles in Tumor Vasculo-Angiogenesis, Invasiveness, CSC-Survival and Anoikis Resistance: A 'Common Receptor Coordinator' Paradigm
    Article Snippet: Panc1 cells, U87 cells, Panc1 CSCs and U87 CSCs were lysed with ice-cold Nuclei EZ lysis buffer (SIGMA-ALDRICH, MO) and supernants containing cytoplasmic RNA were extracted twice with Phenol:Chloroform (50∶50) followed by ethanol precipitation. .. After removal and documentation of no residual DNA contamination, cDNA synthesis was performed with the QuantiTect Reverse Transcription Kit (Qiagen, MD) using 400 ng of total cytoplasmic RNA, a DEspR specific primer (Reverse R188 bp : 5′-TGGACCAGAGAAATTGCTTG-3′ , ) and a Cyclophilin specific primer (Reverse: 5′-GAAGTCACCACCCTGACA-3′ ).

    Spectrophotometry:

    Article Title: Genetic and epigenetic analysis of putative breast cancer stem cell models
    Article Snippet: RNA was quantified and assessed for purity by UV spectrophotometry. .. One microgram of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer’s instructions. qPCR was performed using LightCycler 480 (Roche).

    Article Title: Genotype-dependency of butyrate efficacy in children with congenital chloride diarrhea
    Article Snippet: Total RNA amount was quantified with Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, UK). .. One microgram of total RNA was retro-transcribed with Quantitect Reverse Transcription kit (Qiagen, Germany), cDNA was diluted for downstream applications like quantitative real-time PCR analysis.

    Article Title: Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore
    Article Snippet: The quality of RNA was assessed using the NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, USA). .. The cDNA (from total RNA; 1 μ g/reaction mixture) was synthesized with the QuantiTect® Reverse Transcription Kit (Qiagen) according to manufacturer's instructions.

    Article Title: Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes
    Article Snippet: For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions. .. For each sample 1 μg total RNA was converted to cDNA using the Quantitect reverse transcription kit (Qiagen) according to the manufacturer's instructions.

    Activation Assay:

    Article Title: Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer: Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers
    Article Snippet: Reverse transcription was performed employing the QuantiTect Reverse Transcription Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions. .. Following this step, real time qRT-PCR assays were performed on a Roche LightCycler 2.0 detection system (Roche Diagnostics GmbH, Mannheim, Germany) in 20 μ L volumes in glass capillaries, employing the QuantiTect Probe RT-PCR Kit from Qiagen (Hilden, Germany) according to the manufacturer's instructions.

    Lysis:

    Article Title: The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer
    Article Snippet: Cycloheximide chase assays were performed by exposing cancer cells to 100 μ g/ml cycloheximide (Sigma-Aldrich) for 1, 2, 3, or 4 h and then lysing the cells for immunoblot analysis. .. Cells were exposed to 17AAG and Nutlin-3a, alone or in combination, and collected after 24 h. Total RNA was extracted using the QIAzol lysis reagent and the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). cDNA was generated from purified RNA using a QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer's instructions. cDNAs corresponding to mRNAs encoding MDM2, MDM4, p21, PUMA, BAX, and PIG3 were amplified by PCR using the primers listed in . qRT-PCR was performed in triplicate using SYBR Green Mix (Qiagen) on a 7900HT Fast Real-time PCR System (Applied Bioscience, Foster, CA, USA). .. Relative target mRNA levels were normalized to GAPDH expression as internal efficiency control.

    Article Title: DEspR Roles in Tumor Vasculo-Angiogenesis, Invasiveness, CSC-Survival and Anoikis Resistance: A 'Common Receptor Coordinator' Paradigm
    Article Snippet: Panc1 cells, U87 cells, Panc1 CSCs and U87 CSCs were lysed with ice-cold Nuclei EZ lysis buffer (SIGMA-ALDRICH, MO) and supernants containing cytoplasmic RNA were extracted twice with Phenol:Chloroform (50∶50) followed by ethanol precipitation. .. After removal and documentation of no residual DNA contamination, cDNA synthesis was performed with the QuantiTect Reverse Transcription Kit (Qiagen, MD) using 400 ng of total cytoplasmic RNA, a DEspR specific primer (Reverse R188 bp : 5′-TGGACCAGAGAAATTGCTTG-3′ , ) and a Cyclophilin specific primer (Reverse: 5′-GAAGTCACCACCCTGACA-3′ ).

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    Qiagen q rt pcr
    Increased expression of K14 and basal cell hyperplasia in TG trachea. ( A ) Relative expression of K14 mRNA by <t>Q-RT–PCR</t> in the lung and tracheas from WT littermates of mouse lines D1-1, D1-2 and J7-1 and from TG animals of mouse lines F8-1, D10-1 and D1-3. The primers used in this experiment detected both mouse and human K14 mRNA. Ribosomal S15 and S18 were used as housekeeping genes. Photomicrographs of ( B ) basal cell hyperplasia (arrows) in tracheal epithelium from a TG mouse (I) and scattered basal cells (arrows) in the trachea of a WT mouse (K14 immunoperoxidase stain; bar = 200 μm). Lu = lumen.
    Q Rt Pcr, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q rt pcr/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q rt pcr - by Bioz Stars, 2019-12
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    99
    Qiagen quanti tect sybr green rt pcr master mix
    Confirmation of silencing in TaIPK1 : RNAi lines in T 4 seeds. (A) Free Pi content of control C306 and TaIPK1 :RNAi lines were estimated using colorimetric based assays. (B) Relative fold change of TaIPK1 expression in wheat transgenic lines. RNAi lines from three independent events were subjected to expression analysis at 14 DAA stage. The cDNA templates were prepared from 2 μg of DNase free RNA. <t>qRT-PCR</t> assays were performed using <t>SYBR</t> green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. (C) Total phytic acid in mature wheat grains of transgenic lines (T 4 ). PA was measured in the mature seeds collected from the primary tiller of each line. # Indicates significant differences at p
    Quanti Tect Sybr Green Rt Pcr Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quanti tect sybr green rt pcr master mix/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    quanti tect sybr green rt pcr master mix - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    99
    Qiagen step rt pcr kit
    <t>RT-PCR</t> amplification of the <t>crtB</t> gene in transgenic cassava leaves samples from rep 1, 2 and 3, respectively, showing positive and negative lines for crtB gene. M/L: molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, MS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control
    Step Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/step rt pcr kit/product/Qiagen
    Average 99 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    step rt pcr kit - by Bioz Stars, 2019-12
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    Image Search Results


    Increased expression of K14 and basal cell hyperplasia in TG trachea. ( A ) Relative expression of K14 mRNA by Q-RT–PCR in the lung and tracheas from WT littermates of mouse lines D1-1, D1-2 and J7-1 and from TG animals of mouse lines F8-1, D10-1 and D1-3. The primers used in this experiment detected both mouse and human K14 mRNA. Ribosomal S15 and S18 were used as housekeeping genes. Photomicrographs of ( B ) basal cell hyperplasia (arrows) in tracheal epithelium from a TG mouse (I) and scattered basal cells (arrows) in the trachea of a WT mouse (K14 immunoperoxidase stain; bar = 200 μm). Lu = lumen.

    Journal: Carcinogenesis

    Article Title: Constitutive expression of human keratin 14 gene in mouse lung induces premalignant lesions and squamous differentiation

    doi: 10.1093/carcin/bgn190

    Figure Lengend Snippet: Increased expression of K14 and basal cell hyperplasia in TG trachea. ( A ) Relative expression of K14 mRNA by Q-RT–PCR in the lung and tracheas from WT littermates of mouse lines D1-1, D1-2 and J7-1 and from TG animals of mouse lines F8-1, D10-1 and D1-3. The primers used in this experiment detected both mouse and human K14 mRNA. Ribosomal S15 and S18 were used as housekeeping genes. Photomicrographs of ( B ) basal cell hyperplasia (arrows) in tracheal epithelium from a TG mouse (I) and scattered basal cells (arrows) in the trachea of a WT mouse (K14 immunoperoxidase stain; bar = 200 μm). Lu = lumen.

    Article Snippet: For quantitative reverse transcription polymerase chain reaction (Q-RT–PCR), QuantiTect SYBR® Green PCR Kit (Qiagen) and PCR amplification in MyiQ (Bio-Rad, Richmond, CA) were used according to the protocol provided by the manufacturer.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

    Characterization of CC10-hK14 TG mice. Photomicrographs of normal bronchiolar epithelium from a WT mouse ( A ) (hematoxylin and eosin stain; bar = 200 μm) and focal epithelial hyperplasia (arrow) from TG mouse ( B ) (hematoxylin and eosin stain; bar = 100 μm). Normal bronchiolar epithelium from a young TG mouse ( C ) and squamous metaplasia in a TG mouse ( D ) (hematoxylin and eosin stain; bar = 400 μm). Inset shows a mitotic figure and intercellular bridges. Bronchiolar epithelium from a WT mouse ( E ) negative for K14 (immunoperoxidase stain; bar = 200 μm) and K14 immunoreactivity in basal cells (long arrows) and focal squamous metaplasia (short arrows) in TG bronchiolar epithelium ( F ) (immunoperoxidase stain; bar = 100 μm). CC10 immunorectivity in bronchiolar epithelium from a WT mouse ( G ) and in hyperplastic, thickened epithelium from a TG mouse ( H ) (immunoperoxidase stain; bar = 200 μm). LU = lumen. ( I ) A graph of body weights. Hatched line = WT mice; solid line = TG mice; mo = months. ( J ) Expression of hK14 mRNA expression by Q-RT-PCR with ribosomal S18 as housekeeping gene. Controls included lungs from two lines of WT mice (WT1 and WT2) and BEAS-2B cells (BE). TG tissues included trachea (TRA) and lungs from independent lines I9, E9, E10 and D1 of mice. M = DNA markers. The primers used in this experiment detected only hK14 gene with a 278 bp product. ( K ) A bar graph of relative hK14 mRNA expression by Q-RT–PCR in a trachea and lungs from TG lines I9, E9, E10, D1, J7 and a WT mouse. ( L ) Immunoprecipitation analysis of hK14 protein in human BEAS-2B cells and WT lung (control) and in lungs from TG mouse lines J7, A9 and E9.

    Journal: Carcinogenesis

    Article Title: Constitutive expression of human keratin 14 gene in mouse lung induces premalignant lesions and squamous differentiation

    doi: 10.1093/carcin/bgn190

    Figure Lengend Snippet: Characterization of CC10-hK14 TG mice. Photomicrographs of normal bronchiolar epithelium from a WT mouse ( A ) (hematoxylin and eosin stain; bar = 200 μm) and focal epithelial hyperplasia (arrow) from TG mouse ( B ) (hematoxylin and eosin stain; bar = 100 μm). Normal bronchiolar epithelium from a young TG mouse ( C ) and squamous metaplasia in a TG mouse ( D ) (hematoxylin and eosin stain; bar = 400 μm). Inset shows a mitotic figure and intercellular bridges. Bronchiolar epithelium from a WT mouse ( E ) negative for K14 (immunoperoxidase stain; bar = 200 μm) and K14 immunoreactivity in basal cells (long arrows) and focal squamous metaplasia (short arrows) in TG bronchiolar epithelium ( F ) (immunoperoxidase stain; bar = 100 μm). CC10 immunorectivity in bronchiolar epithelium from a WT mouse ( G ) and in hyperplastic, thickened epithelium from a TG mouse ( H ) (immunoperoxidase stain; bar = 200 μm). LU = lumen. ( I ) A graph of body weights. Hatched line = WT mice; solid line = TG mice; mo = months. ( J ) Expression of hK14 mRNA expression by Q-RT-PCR with ribosomal S18 as housekeeping gene. Controls included lungs from two lines of WT mice (WT1 and WT2) and BEAS-2B cells (BE). TG tissues included trachea (TRA) and lungs from independent lines I9, E9, E10 and D1 of mice. M = DNA markers. The primers used in this experiment detected only hK14 gene with a 278 bp product. ( K ) A bar graph of relative hK14 mRNA expression by Q-RT–PCR in a trachea and lungs from TG lines I9, E9, E10, D1, J7 and a WT mouse. ( L ) Immunoprecipitation analysis of hK14 protein in human BEAS-2B cells and WT lung (control) and in lungs from TG mouse lines J7, A9 and E9.

    Article Snippet: For quantitative reverse transcription polymerase chain reaction (Q-RT–PCR), QuantiTect SYBR® Green PCR Kit (Qiagen) and PCR amplification in MyiQ (Bio-Rad, Richmond, CA) were used according to the protocol provided by the manufacturer.

    Techniques: Mouse Assay, H&E Stain, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation

    Expression of CCE precursor mRNAs in the lungs and trachea of TG mice. Relative expression of involucrin, loricrin, transglutaminase 1 (TGase 1), small-proline rich protein 1A (SPRR 1A) and cholesterol sulfotransferase 2B1 (SULT2B1) mRNAs was analyzed by Q-RT–PCR in the lung tissue of WT mouse and in the trachea (TRA) and lungs from CC10-hK14 TG mouse lines I9, E9, E10, D1 and J7. Ribosomal S15 and S18 were used as housekeeping genes.

    Journal: Carcinogenesis

    Article Title: Constitutive expression of human keratin 14 gene in mouse lung induces premalignant lesions and squamous differentiation

    doi: 10.1093/carcin/bgn190

    Figure Lengend Snippet: Expression of CCE precursor mRNAs in the lungs and trachea of TG mice. Relative expression of involucrin, loricrin, transglutaminase 1 (TGase 1), small-proline rich protein 1A (SPRR 1A) and cholesterol sulfotransferase 2B1 (SULT2B1) mRNAs was analyzed by Q-RT–PCR in the lung tissue of WT mouse and in the trachea (TRA) and lungs from CC10-hK14 TG mouse lines I9, E9, E10, D1 and J7. Ribosomal S15 and S18 were used as housekeeping genes.

    Article Snippet: For quantitative reverse transcription polymerase chain reaction (Q-RT–PCR), QuantiTect SYBR® Green PCR Kit (Qiagen) and PCR amplification in MyiQ (Bio-Rad, Richmond, CA) were used according to the protocol provided by the manufacturer.

    Techniques: Expressing, Mouse Assay, Reverse Transcription Polymerase Chain Reaction

    Confirmation of silencing in TaIPK1 : RNAi lines in T 4 seeds. (A) Free Pi content of control C306 and TaIPK1 :RNAi lines were estimated using colorimetric based assays. (B) Relative fold change of TaIPK1 expression in wheat transgenic lines. RNAi lines from three independent events were subjected to expression analysis at 14 DAA stage. The cDNA templates were prepared from 2 μg of DNase free RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. (C) Total phytic acid in mature wheat grains of transgenic lines (T 4 ). PA was measured in the mature seeds collected from the primary tiller of each line. # Indicates significant differences at p

    Journal: Frontiers in Plant Science

    Article Title: RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation

    doi: 10.3389/fpls.2018.00259

    Figure Lengend Snippet: Confirmation of silencing in TaIPK1 : RNAi lines in T 4 seeds. (A) Free Pi content of control C306 and TaIPK1 :RNAi lines were estimated using colorimetric based assays. (B) Relative fold change of TaIPK1 expression in wheat transgenic lines. RNAi lines from three independent events were subjected to expression analysis at 14 DAA stage. The cDNA templates were prepared from 2 μg of DNase free RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. (C) Total phytic acid in mature wheat grains of transgenic lines (T 4 ). PA was measured in the mature seeds collected from the primary tiller of each line. # Indicates significant differences at p

    Article Snippet: For the confirmation of gene silencing, SYBR Green (Quanti-Tect SYBR Green RT-PCR Master mix, QIAGEN) based reactions were performed on ABI PRISM 7500 Fast Real-Time Platform (Applied Biosystems).

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR, SYBR Green Assay

    Differential expression analysis of three homoeologs of TaIPK1 at two seed developmental stages (14 and 21 DAA). Transcript specific primers were designed for 2AL, 2BL, 2DL of TaIPK1 homoeologs based on genomic information available at IWGSC. gDNA free cDNA was prepared using 2 μg of RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. The indicated error bars represents the standard deviation from three independent replicates.

    Journal: Frontiers in Plant Science

    Article Title: RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation

    doi: 10.3389/fpls.2018.00259

    Figure Lengend Snippet: Differential expression analysis of three homoeologs of TaIPK1 at two seed developmental stages (14 and 21 DAA). Transcript specific primers were designed for 2AL, 2BL, 2DL of TaIPK1 homoeologs based on genomic information available at IWGSC. gDNA free cDNA was prepared using 2 μg of RNA. qRT-PCR assays were performed using SYBR green and C t values were normalized against wheat ADP-ribosylation factor 1 ( ARF1 ) as an internal control. The indicated error bars represents the standard deviation from three independent replicates.

    Article Snippet: For the confirmation of gene silencing, SYBR Green (Quanti-Tect SYBR Green RT-PCR Master mix, QIAGEN) based reactions were performed on ABI PRISM 7500 Fast Real-Time Platform (Applied Biosystems).

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay, Standard Deviation

    RT-PCR amplification of the crtB gene in transgenic cassava leaves samples from rep 1, 2 and 3, respectively, showing positive and negative lines for crtB gene. M/L: molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, MS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Journal: 3 Biotech

    Article Title: Gene expression of beta carotene genes in transgenic biofortified cassava

    doi: 10.1007/s13205-014-0243-8

    Figure Lengend Snippet: RT-PCR amplification of the crtB gene in transgenic cassava leaves samples from rep 1, 2 and 3, respectively, showing positive and negative lines for crtB gene. M/L: molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, MS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Article Snippet: RNA was isolated from cassava roots and leaves using a modified Dellaporta protocol, analyzed for expression of DXS, crtB and the selectable marker, npt II using the one step RT-PCR kit (Qiagen) and analyzed through gel electrophoresis.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Transgenic Assay, Mass Spectrometry, Negative Control, Positive Control

    RT-PCR amplification of the npt II gene in transgenic cassava leaves samples from rep 1, 2 and 3, respectively, showing positive and negative lines for npt II gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Journal: 3 Biotech

    Article Title: Gene expression of beta carotene genes in transgenic biofortified cassava

    doi: 10.1007/s13205-014-0243-8

    Figure Lengend Snippet: RT-PCR amplification of the npt II gene in transgenic cassava leaves samples from rep 1, 2 and 3, respectively, showing positive and negative lines for npt II gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Article Snippet: RNA was isolated from cassava roots and leaves using a modified Dellaporta protocol, analyzed for expression of DXS, crtB and the selectable marker, npt II using the one step RT-PCR kit (Qiagen) and analyzed through gel electrophoresis.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Transgenic Assay, Negative Control, Positive Control

    RT-PCR amplification of the DXS gene in transgenic roots obtained from rep 1, 2 and 3, respectively, showing the positive and negative lines for the DXS gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Journal: 3 Biotech

    Article Title: Gene expression of beta carotene genes in transgenic biofortified cassava

    doi: 10.1007/s13205-014-0243-8

    Figure Lengend Snippet: RT-PCR amplification of the DXS gene in transgenic roots obtained from rep 1, 2 and 3, respectively, showing the positive and negative lines for the DXS gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Article Snippet: RNA was isolated from cassava roots and leaves using a modified Dellaporta protocol, analyzed for expression of DXS, crtB and the selectable marker, npt II using the one step RT-PCR kit (Qiagen) and analyzed through gel electrophoresis.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Transgenic Assay, Negative Control, Positive Control

    RT-PCR amplification of the crtB gene in transgenic roots in rep 1, 2 and 3, respectively, showing the positive and negative lines for the crtB gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Journal: 3 Biotech

    Article Title: Gene expression of beta carotene genes in transgenic biofortified cassava

    doi: 10.1007/s13205-014-0243-8

    Figure Lengend Snippet: RT-PCR amplification of the crtB gene in transgenic roots in rep 1, 2 and 3, respectively, showing the positive and negative lines for the crtB gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Article Snippet: RNA was isolated from cassava roots and leaves using a modified Dellaporta protocol, analyzed for expression of DXS, crtB and the selectable marker, npt II using the one step RT-PCR kit (Qiagen) and analyzed through gel electrophoresis.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Transgenic Assay, Negative Control, Positive Control

    RT-PCR amplification of the DXS gene in transgenic cassava leaves in samples from rep 1, 2 and 3, respectively, showing positive and negative lines for DXS gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Journal: 3 Biotech

    Article Title: Gene expression of beta carotene genes in transgenic biofortified cassava

    doi: 10.1007/s13205-014-0243-8

    Figure Lengend Snippet: RT-PCR amplification of the DXS gene in transgenic cassava leaves in samples from rep 1, 2 and 3, respectively, showing positive and negative lines for DXS gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Article Snippet: RNA was isolated from cassava roots and leaves using a modified Dellaporta protocol, analyzed for expression of DXS, crtB and the selectable marker, npt II using the one step RT-PCR kit (Qiagen) and analyzed through gel electrophoresis.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Transgenic Assay, Negative Control, Positive Control

    RT-PCR amplification of the npt II gene in transgenic roots in rep 1, 2, and 3, respectively, showing positive and negative lines for the npt II gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Journal: 3 Biotech

    Article Title: Gene expression of beta carotene genes in transgenic biofortified cassava

    doi: 10.1007/s13205-014-0243-8

    Figure Lengend Snippet: RT-PCR amplification of the npt II gene in transgenic roots in rep 1, 2, and 3, respectively, showing positive and negative lines for the npt II gene. M/L: Molecular ladder 100 bp (GeneRuler); 2, 20, 37 and 73: Transgenic lines, TMS: TMS 60444 non-transgenic cultivar; LOC: Local check ( yellow fleshed ); −VE– Negative control ; +VE– Positive control

    Article Snippet: RNA was isolated from cassava roots and leaves using a modified Dellaporta protocol, analyzed for expression of DXS, crtB and the selectable marker, npt II using the one step RT-PCR kit (Qiagen) and analyzed through gel electrophoresis.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Transgenic Assay, Negative Control, Positive Control

    RT-PCR analysis of total RNA from control (C) and moderate DM2 ( DM2 ) skeletal muscle tissue using oligonucleotide complementary to exons 5 and 10 (CLCN3F 5'-ggcagtggcatccccgtgggg-3' and CLCN3R 5'-cagctcccaggagcccacag- 3'). Two bands were distinguished: the standard expected product (S) 414 bp and the product excluding the exons 6 and 7, D6-7 ( VAR ) 257 bp. Additional discrete visible bands may be due to the RT-PCR condition and false primer annealing and were not ClC1 isoforms, amplicons from different genes as confirmed by subsequent sequencing. B) Sequencing of D6-7 variant showing exclusion of exons 6 and 7. C) Densitometry results suggested that in controls the ratio of normal length-variant vs. D6-7 was 5.7:1 and in DM2 1:4. Error bars represent standard deviation.

    Journal: Acta Myologica

    Article Title: ClC1 chloride channel in myotonic dystrophy type 2 and ClC1 splicing in vitro

    doi:

    Figure Lengend Snippet: RT-PCR analysis of total RNA from control (C) and moderate DM2 ( DM2 ) skeletal muscle tissue using oligonucleotide complementary to exons 5 and 10 (CLCN3F 5'-ggcagtggcatccccgtgggg-3' and CLCN3R 5'-cagctcccaggagcccacag- 3'). Two bands were distinguished: the standard expected product (S) 414 bp and the product excluding the exons 6 and 7, D6-7 ( VAR ) 257 bp. Additional discrete visible bands may be due to the RT-PCR condition and false primer annealing and were not ClC1 isoforms, amplicons from different genes as confirmed by subsequent sequencing. B) Sequencing of D6-7 variant showing exclusion of exons 6 and 7. C) Densitometry results suggested that in controls the ratio of normal length-variant vs. D6-7 was 5.7:1 and in DM2 1:4. Error bars represent standard deviation.

    Article Snippet: RT-PCR amplification was carried out in a final volume of 50 μl, using equal amounts (1-2 μg) of total RNA, and 50 pmol upstream and downstream primers with an one step RT-PCR kit (Qiagen, Hilden, Germany).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Variant Assay, Standard Deviation

    RT-PCR assay of CLCN1 exons 3 to 10, from RNA extracted after expression of different repeat RNAs: (AAG)24 (A), (CCUG)18 (B) and (CUG)24 (C), on days 2 and 3 after transfection. The upper band, S, represents the standard RT-PCR product; • represents skipping of exons 6-9; ** represents skipping of exons 6-7. D) RT-PCR from a series of RNA dilutions of total RNA extracted from transfected C2C12 cells on day 3 after transfection suggested that (CCUG)18 and (CUG)24 RNA levels are similar, while (AAG)24 RNA levels are only about 33% of this value.

    Journal: Acta Myologica

    Article Title: ClC1 chloride channel in myotonic dystrophy type 2 and ClC1 splicing in vitro

    doi:

    Figure Lengend Snippet: RT-PCR assay of CLCN1 exons 3 to 10, from RNA extracted after expression of different repeat RNAs: (AAG)24 (A), (CCUG)18 (B) and (CUG)24 (C), on days 2 and 3 after transfection. The upper band, S, represents the standard RT-PCR product; • represents skipping of exons 6-9; ** represents skipping of exons 6-7. D) RT-PCR from a series of RNA dilutions of total RNA extracted from transfected C2C12 cells on day 3 after transfection suggested that (CCUG)18 and (CUG)24 RNA levels are similar, while (AAG)24 RNA levels are only about 33% of this value.

    Article Snippet: RT-PCR amplification was carried out in a final volume of 50 μl, using equal amounts (1-2 μg) of total RNA, and 50 pmol upstream and downstream primers with an one step RT-PCR kit (Qiagen, Hilden, Germany).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection

    Summary of splice variants of CLCN1 -RNA in the m-RNA region between exons 5 and 8 comparing different studies with our data ( 9 , 10 ). The positions of the pre-mature stop codons of the splicing variants are indicated. The last line,

    Journal: Acta Myologica

    Article Title: ClC1 chloride channel in myotonic dystrophy type 2 and ClC1 splicing in vitro

    doi:

    Figure Lengend Snippet: Summary of splice variants of CLCN1 -RNA in the m-RNA region between exons 5 and 8 comparing different studies with our data ( 9 , 10 ). The positions of the pre-mature stop codons of the splicing variants are indicated. The last line, "other variants", refers to other splicing events affecting the region without being directly displayable in the scheme (e.g. exclusion of exons 4-7 or 2-12 etc). n.d. = not detected. B) RT-PCR analysis of total RNA from a very mild case of DM2 and a control using the same technique as figure 1 . The standard expected product (S) of 414 bp and the product excluding the exons 6 and 7, D6-7 ( VAR ) of 257 bp are shown after 5, 10, 15, 20, 25, 30, and 35 PCR cycles. Densitometry suggested that in mild DM2, the ratio of normal-length variant vs. D6-7 was 58:42 average over all cycle numbers.

    Article Snippet: RT-PCR amplification was carried out in a final volume of 50 μl, using equal amounts (1-2 μg) of total RNA, and 50 pmol upstream and downstream primers with an one step RT-PCR kit (Qiagen, Hilden, Germany).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Variant Assay