quantitect probe pcr kit  (Qiagen)


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    Structured Review

    Qiagen quantitect probe pcr kit
    Quantitect Probe Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect probe pcr kit/product/Qiagen
    Average 98 stars, based on 142 article reviews
    Price from $9.99 to $1999.99
    quantitect probe pcr kit - by Bioz Stars, 2020-04
    98/100 stars

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    Related Articles

    Negative Control:

    Article Title: Mass gathering and globalization of respiratory pathogens during the 2013 Hajj.
    Article Snippet: Detection of respiratory bacteria and Pneumocystis jirovecii All real-time PCRs were performed using a 7900HT-thermocycler (Applied Biosystems, USA) and QuantiTect-Probe PCR Kit (Qiagen, France) according to the manufacturer's recommendations. .. A negative control (sterile water) and positive control (DNA from the bacterial strain) were included in each run.

    Amplification:

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: Real-Time Quantitative RT-PCR To quantify the influenza viral load and cytokine mRNA and apoptosis-associated mRNA expression in lung tissues, a real-time quantitative RT-PCR (RT-qPCR) was performed using the QPCR detection system (Agilent Technologies Inc., Santa Clara, CA) using a fluorescently labeled TaqMan probe to enable continuous monitoring of amplicon formation. .. 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe.

    Positive Control:

    Article Title: Mass gathering and globalization of respiratory pathogens during the 2013 Hajj.
    Article Snippet: Detection of respiratory bacteria and Pneumocystis jirovecii All real-time PCRs were performed using a 7900HT-thermocycler (Applied Biosystems, USA) and QuantiTect-Probe PCR Kit (Qiagen, France) according to the manufacturer's recommendations. .. A negative control (sterile water) and positive control (DNA from the bacterial strain) were included in each run.

    Fluorescence:

    Article Title: Identification of the polymorphisms in IFITM1 gene and their association in a Korean population with ulcerative colitis.
    Article Snippet: The 10 l reaction mixture comprised 2 l of genomic DNA (50 ng), 1 l of primer mix (containing 5 pmol of the forward and reverse primers), 0.25 l of EvaGreen solution (Biotium, Hayward, CA, USA) and 5 l of a QuantiTect Probe PCR kit (Qiagen, Valencia, CA, USA). .. The PCR cycling conditions were as follows: 1 cycle at 95 • C for 15 min; 45 cycles at 95 • C for 5 s; annealing conditions at 60 • C for 10 s; and 72 • C for 30 s. After a completion of 45 cycles, melting curve data were generated by increasing the temperature from 77 to 95 • C at 0.1 • C/s and recording fluorescence.

    Serial Dilution:

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe. .. A standard curve was generated using serial dilution of an in vitro transcribed standard RNA (from approximately 10 to 10 7 copies) or A549 cell RNA (from approximately 0.1 pg to 100 ng).

    Immunohistochemistry:

    Article Title: Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S.
    Article Snippet: Paragraph title: Necropsy, qPCR, histology and immunohistochemistry ... For real-time PCR (qPCR) the QuantiTect probe PCR kit (Qiagen) was used.

    Quantitative RT-PCR:

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: Paragraph title: Real-Time Quantitative RT-PCR ... 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe.

    Real-time Polymerase Chain Reaction:

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: Real-Time Quantitative RT-PCR To quantify the influenza viral load and cytokine mRNA and apoptosis-associated mRNA expression in lung tissues, a real-time quantitative RT-PCR (RT-qPCR) was performed using the QPCR detection system (Agilent Technologies Inc., Santa Clara, CA) using a fluorescently labeled TaqMan probe to enable continuous monitoring of amplicon formation. .. 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe.

    Article Title: Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S.
    Article Snippet: .. For real-time PCR (qPCR) the QuantiTect probe PCR kit (Qiagen) was used. ..

    Generated:

    Article Title: Identification of the polymorphisms in IFITM1 gene and their association in a Korean population with ulcerative colitis.
    Article Snippet: The 10 l reaction mixture comprised 2 l of genomic DNA (50 ng), 1 l of primer mix (containing 5 pmol of the forward and reverse primers), 0.25 l of EvaGreen solution (Biotium, Hayward, CA, USA) and 5 l of a QuantiTect Probe PCR kit (Qiagen, Valencia, CA, USA). .. The PCR cycling conditions were as follows: 1 cycle at 95 • C for 15 min; 45 cycles at 95 • C for 5 s; annealing conditions at 60 • C for 10 s; and 72 • C for 30 s. After a completion of 45 cycles, melting curve data were generated by increasing the temperature from 77 to 95 • C at 0.1 • C/s and recording fluorescence.

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe. .. A standard curve was generated using serial dilution of an in vitro transcribed standard RNA (from approximately 10 to 10 7 copies) or A549 cell RNA (from approximately 0.1 pg to 100 ng).

    Labeling:

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: Real-Time Quantitative RT-PCR To quantify the influenza viral load and cytokine mRNA and apoptosis-associated mRNA expression in lung tissues, a real-time quantitative RT-PCR (RT-qPCR) was performed using the QPCR detection system (Agilent Technologies Inc., Santa Clara, CA) using a fluorescently labeled TaqMan probe to enable continuous monitoring of amplicon formation. .. 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe.

    Expressing:

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: Real-Time Quantitative RT-PCR To quantify the influenza viral load and cytokine mRNA and apoptosis-associated mRNA expression in lung tissues, a real-time quantitative RT-PCR (RT-qPCR) was performed using the QPCR detection system (Agilent Technologies Inc., Santa Clara, CA) using a fluorescently labeled TaqMan probe to enable continuous monitoring of amplicon formation. .. 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe.

    Isolation:

    Article Title: Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S.
    Article Snippet: Samples (Table 1A) were frozen at −80°C until DNA was isolated using the QIAamp DNA Mini Kit (Qiagen) according to the manufacturer's instructions. .. For real-time PCR (qPCR) the QuantiTect probe PCR kit (Qiagen) was used.

    Polymerase Chain Reaction:

    Article Title: Mass gathering and globalization of respiratory pathogens during the 2013 Hajj.
    Article Snippet: .. Detection of respiratory bacteria and Pneumocystis jirovecii All real-time PCRs were performed using a 7900HT-thermocycler (Applied Biosystems, USA) and QuantiTect-Probe PCR Kit (Qiagen, France) according to the manufacturer's recommendations. ..

    Article Title: Identification of the polymorphisms in IFITM1 gene and their association in a Korean population with ulcerative colitis.
    Article Snippet: .. The 10 l reaction mixture comprised 2 l of genomic DNA (50 ng), 1 l of primer mix (containing 5 pmol of the forward and reverse primers), 0.25 l of EvaGreen solution (Biotium, Hayward, CA, USA) and 5 l of a QuantiTect Probe PCR kit (Qiagen, Valencia, CA, USA). .. PCR cycling and HRM analysis were performed on the Gene TM 6000 (Corbett Research).

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: .. 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe. .. A standard curve was generated using serial dilution of an in vitro transcribed standard RNA (from approximately 10 to 10 7 copies) or A549 cell RNA (from approximately 0.1 pg to 100 ng).

    Article Title: Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S.
    Article Snippet: .. For real-time PCR (qPCR) the QuantiTect probe PCR kit (Qiagen) was used. ..

    Staining:

    Article Title: Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S.
    Article Snippet: For real-time PCR (qPCR) the QuantiTect probe PCR kit (Qiagen) was used. .. Necropsy, qPCR, histology and immunohistochemistry For histology, tissues ( Fig. 1 ) were paraformaldehyde-fixed, paraffin-embedded, cut and sections were stained with hemalum-eosin.

    Software:

    Article Title: Identification of the polymorphisms in IFITM1 gene and their association in a Korean population with ulcerative colitis.
    Article Snippet: The 10 l reaction mixture comprised 2 l of genomic DNA (50 ng), 1 l of primer mix (containing 5 pmol of the forward and reverse primers), 0.25 l of EvaGreen solution (Biotium, Hayward, CA, USA) and 5 l of a QuantiTect Probe PCR kit (Qiagen, Valencia, CA, USA). .. HRM curve analysis was performed using the Rotor-Gene 1.7.40 software and the HRM algorithm that was provided.

    In Vitro:

    Article Title: Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis.
    Article Snippet: 23e28 The assays were performed in a total volume of 25 mL using the QuantiTect Probe PCR Kit (Qiagen), 5 mL total RNA, 40 mmol/L each primer, and 20 mmol/L each probe. .. A standard curve was generated using serial dilution of an in vitro transcribed standard RNA (from approximately 10 to 10 7 copies) or A549 cell RNA (from approximately 0.1 pg to 100 ng).

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    Qiagen quantitect multiplex pcr norox master mix
    Results obtained in cross-reactivity tests performed with DNA isolates from 23 animal species with 5 commercial master mixes. ( A ) <t>QuantiTect®</t> Multiplex <t>PCR</t> <t>NoROX</t> Master Mix (Qiagen), ( B ) TaqMan® Universal PCR Master Mix (Applied Biosystems), ( C ) GoTaq® Probe qPCR Master Mix (Promega), ( D ) PerfeCTa® qPCR ToughMix TM , Low ROX TM (Quanta Biosciences) and E) Takyon TM No Rox Probe MasterMix dTTP Blue (Eurogentec).
    Quantitect Multiplex Pcr Norox Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect multiplex pcr norox master mix/product/Qiagen
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    quantitect multiplex pcr norox master mix - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen quantitect taqman probe rt pcr kit
    Quantitative <t>TaqMan</t> <t>RT-PCR</t>
    Quantitect Taqman Probe Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect taqman probe rt pcr kit/product/Qiagen
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    quantitect taqman probe rt pcr kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Results obtained in cross-reactivity tests performed with DNA isolates from 23 animal species with 5 commercial master mixes. ( A ) QuantiTect® Multiplex PCR NoROX Master Mix (Qiagen), ( B ) TaqMan® Universal PCR Master Mix (Applied Biosystems), ( C ) GoTaq® Probe qPCR Master Mix (Promega), ( D ) PerfeCTa® qPCR ToughMix TM , Low ROX TM (Quanta Biosciences) and E) Takyon TM No Rox Probe MasterMix dTTP Blue (Eurogentec).

    Journal: Scientific Reports

    Article Title: Sika deer (Cervus nippon)-specific real-time PCR method to detect fraudulent labelling of meat and meat products

    doi: 10.1038/s41598-018-25299-7

    Figure Lengend Snippet: Results obtained in cross-reactivity tests performed with DNA isolates from 23 animal species with 5 commercial master mixes. ( A ) QuantiTect® Multiplex PCR NoROX Master Mix (Qiagen), ( B ) TaqMan® Universal PCR Master Mix (Applied Biosystems), ( C ) GoTaq® Probe qPCR Master Mix (Promega), ( D ) PerfeCTa® qPCR ToughMix TM , Low ROX TM (Quanta Biosciences) and E) Takyon TM No Rox Probe MasterMix dTTP Blue (Eurogentec).

    Article Snippet: The other two master mixes, the QuantiTect Multiplex PCR NoROX Master Mix (Qiagen) and the TaqMan® Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) were used by applying conventional thermal cycling programs.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Results obtained in cross-reactivity tests performed with DNA isolates from 23 animal species with 5 commercial master mixes. ( A ) QuantiTect® Multiplex PCR NoROX Master Mix (Qiagen), ( B ) TaqMan® Universal PCR Master Mix (Applied Biosystems), ( C ) GoTaq® Probe qPCR Master Mix (Promega), ( D ) PerfeCTa® qPCR ToughMix TM , Low ROX TM (Quanta Biosciences) and E) Takyon TM No Rox Probe MasterMix dTTP Blue (Eurogentec).

    Journal: Scientific Reports

    Article Title: Sika deer (Cervus nippon)-specific real-time PCR method to detect fraudulent labelling of meat and meat products

    doi: 10.1038/s41598-018-25299-7

    Figure Lengend Snippet: Results obtained in cross-reactivity tests performed with DNA isolates from 23 animal species with 5 commercial master mixes. ( A ) QuantiTect® Multiplex PCR NoROX Master Mix (Qiagen), ( B ) TaqMan® Universal PCR Master Mix (Applied Biosystems), ( C ) GoTaq® Probe qPCR Master Mix (Promega), ( D ) PerfeCTa® qPCR ToughMix TM , Low ROX TM (Quanta Biosciences) and E) Takyon TM No Rox Probe MasterMix dTTP Blue (Eurogentec).

    Article Snippet: Unless indicated differently, the reaction mix consisted of 12.5 µL QuantiTect Multiplex PCR NoROX Master Mix (Qiagen), 2.5 µL ultrapure water, 5 µL 5-fold concentrated primer/probe mix and 5 µL DNA isolate (DNA concentration between 5 ng/µL and 20 ng/µL).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Quantitative TaqMan RT-PCR

    Journal:

    Article Title: Quantitative expression of the Candida albicans secreted aspartyl proteinase gene family in human oral and vaginal candidiasis

    doi: 10.1099/mic.0.2008/022293-0

    Figure Lengend Snippet: Quantitative TaqMan RT-PCR

    Article Snippet: Real-time RT-PCR was used to determine the quantitative levels of SAP1–10 mRNA transcripts in RNA samples using the GeneAmp ABI 5700 and 7500 PCR machines (PE Applied Biosystems) and the QuantiTect TaqMan probe RT-PCR kit (Qiagen) according to the manufacturer’s instructions.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    qRT-PCR

    Journal: Journal of food protection

    Article Title: Inactivation of the Tulane Virus, a Novel Surrogate for the Human Norovirus

    doi: 10.4315/0362-028X.JFP-12-361

    Figure Lengend Snippet: qRT-PCR

    Article Snippet: Extracted viral RNA was quantitated with probe-based qRT-PCR by using a qPCR system (MX3000P, Stratagene, La Jolla, CA) with a one-step qRT-PCR kit (QuantiTect Probe RT-PCR Kit, QIAGEN) in accordance with the manufacturer’s protocol.

    Techniques: Quantitative RT-PCR