quantitec reverse transcription kit  (Qiagen)

 
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    Name:
    QuantiTect Rev Transcription Kit
    Description:
    For fast cDNA synthesis enabling sensitive real time two step RT PCR for gene expression analysis Kit contents Qiagen QuantiTect Reverse Transcription Kit 10 x 20L rxns RNA Template Sample Two step cDNA Production Genomic DNA Digestion Reaction Type For Fast cDNA Synthesis Enabling Sensitive Real time Two step RT PCR for Gene Expression Analysis Includes 10 x 20L rxns 100L 7x gDNA Wipeout Buffer 10L Quantiscript Reverse Transcriptase 200L 5x Quantiscript RT Buffer 50L RT Primer Mix 1 9mL RNase free Water Benefits cDNA synthesis and gDNA removal in only 20 minutes High cDNA yields even from low abundance transcripts cDNA synthesis from 5 and 3 regions of transcripts No need to design RNA specific primers or prob
    Catalog Number:
    205310
    Price:
    101
    Category:
    QuantiTect Reverse Transcription Kit
    Buy from Supplier


    Structured Review

    Qiagen quantitec reverse transcription kit
    QuantiTect Rev Transcription Kit
    For fast cDNA synthesis enabling sensitive real time two step RT PCR for gene expression analysis Kit contents Qiagen QuantiTect Reverse Transcription Kit 10 x 20L rxns RNA Template Sample Two step cDNA Production Genomic DNA Digestion Reaction Type For Fast cDNA Synthesis Enabling Sensitive Real time Two step RT PCR for Gene Expression Analysis Includes 10 x 20L rxns 100L 7x gDNA Wipeout Buffer 10L Quantiscript Reverse Transcriptase 200L 5x Quantiscript RT Buffer 50L RT Primer Mix 1 9mL RNase free Water Benefits cDNA synthesis and gDNA removal in only 20 minutes High cDNA yields even from low abundance transcripts cDNA synthesis from 5 and 3 regions of transcripts No need to design RNA specific primers or prob
    https://www.bioz.com/result/quantitec reverse transcription kit/product/Qiagen
    Average 99 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    quantitec reverse transcription kit - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties
    Article Snippet: .. Expression studies by RT-PCR and RT-qPCR Expression studies of a few representative AFL and BRL genes relevant to the study’s objectives were performed by RT-PCR and RT-qPCR using the cDNA prepared with QuantiTect Reverse-Transcription Kit (Qiagen), as described above. .. Gene-specific primers not less than 20 nt long and with Tm values no less than 59.0°C were designed using the NCBI Primer Blast software ( http://www.ncbi.nlm.nih.gov/tools/primer-blast ).

    Spectrophotometry:

    Article Title: FITC Conjugation Markedly Enhances Hepatic Clearance of N-Formyl Peptides
    Article Snippet: .. The quantity and quality of the extracted RNA was determined by the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer's protocol. .. Quantitative PCR assay PCR was performed in a 25 μl reaction mixture containing 2 μl of cDNA (from isolated RNA), 12.5 μl of the JumpStart REDTaq® Ready Mix (Sigma-Aldrich), 0.5 μl of 10 μM forward and reverse primers, and 9.5 μl of ddH2 O.

    Expressing:

    Article Title: Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties
    Article Snippet: .. Expression studies by RT-PCR and RT-qPCR Expression studies of a few representative AFL and BRL genes relevant to the study’s objectives were performed by RT-PCR and RT-qPCR using the cDNA prepared with QuantiTect Reverse-Transcription Kit (Qiagen), as described above. .. Gene-specific primers not less than 20 nt long and with Tm values no less than 59.0°C were designed using the NCBI Primer Blast software ( http://www.ncbi.nlm.nih.gov/tools/primer-blast ).

    Quantitative RT-PCR:

    Article Title: Identification and Characterization of Differentially Expressed Genes in Inferior and Superior Spikelets of Rice Cultivars with Contrasting Panicle-Compactness and Grain-Filling Properties
    Article Snippet: .. Expression studies by RT-PCR and RT-qPCR Expression studies of a few representative AFL and BRL genes relevant to the study’s objectives were performed by RT-PCR and RT-qPCR using the cDNA prepared with QuantiTect Reverse-Transcription Kit (Qiagen), as described above. .. Gene-specific primers not less than 20 nt long and with Tm values no less than 59.0°C were designed using the NCBI Primer Blast software ( http://www.ncbi.nlm.nih.gov/tools/primer-blast ).

    Synthesized:

    Article Title: Neonatal Infection Produces Significant Changes in Immune Function with No Associated Learning Deficits in Juvenile Rats
    Article Snippet: .. Genomic DNA was eliminated and cDNA was synthesized from extracted RNA (1,000 ng/ μ L) using the QuantiTect Reverse Transcription Kit (Cat. No. 205314, Qiagen). .. Relative gene expression was quantified by real-time PCR using the RealMasterMix Fast SYBR Kit (Cat. No. 2200830, 5 Prime) in 10 μL reactions on a CFX96Touch real time PCR machine.

    Article Title: FITC Conjugation Markedly Enhances Hepatic Clearance of N-Formyl Peptides
    Article Snippet: .. The quantity and quality of the extracted RNA was determined by the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer's protocol. .. Quantitative PCR assay PCR was performed in a 25 μl reaction mixture containing 2 μl of cDNA (from isolated RNA), 12.5 μl of the JumpStart REDTaq® Ready Mix (Sigma-Aldrich), 0.5 μl of 10 μM forward and reverse primers, and 9.5 μl of ddH2 O.

    Article Title: A Myostatin Inhibitor (Propeptide-Fc) Increases Muscle Mass and Muscle Fiber Size in Aged Mice but Does not Increase Bone Density or Bone Strength
    Article Snippet: .. Total RNA was extracted using Trizol and cDNA was synthesized using Quantitect reverse transcription kit (catalog no. 205310; Qiagen). .. Expression was analyzed quantitatively by means of the Quantitect SYBR Green PCR kit (catalog no. 204143, Qiagen), and QuantiTect Primer Assays.

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    Qiagen miscript reverse transcription kit
    Role of miR-146 family in up-regulation of MAT2A expression in TAMR-MCF-7 cells A. Down-regulation of miR-146a and miR-146b expression in TAMR-MCF-7 cells. miR-146a and miR-146b expression in MCF-7 and TAMR-MCF-7 cells were determined by using <t>miScript</t> PCR kit with miScript primers specific for mature miR-146a and miR-146b. Samples were normalized to small nRNA U6. B. Effects of miR-146a and miR-146b mimics on the miR-146a/b expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics for 36 h (120 p mole/well). miR-146a and miR-146b levels were determined by using miScript PCR kit with miScript primers specific for mature miR-146a and miR-146b. Samples were normalized to small nRNA U6. C. Effects of miR-146a/b mimic on NF-κB activity and MAT2A protein expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics (120 p mole/well) for 36 h and immunoblottings were performed. The data were confirmed by two independent experiments. D. Effects of miR-146b mimic on MAT2A mRNA expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics (120 p mole/well) for 36 h. MAT2A mRNA levels were determined by quantitative RT-PCR. Data represent mean ± SD with 3 different samples (significant versus control mimic miR-treated TAMR-MCF-7 cells, * P
    Miscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miscript reverse transcription kit/product/Qiagen
    Average 95 stars, based on 429 article reviews
    Price from $9.99 to $1999.99
    miscript reverse transcription kit - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    99
    Qiagen quantitect reverse transcription kit
    RT q PCR analysis showing relative gene expression levels of IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, TNF ‐α, and IFN ‐γ in allograft skin (A – H) and muscle (I – P). For RT q PCR , a total volume of 15 μl per reaction was used containing 50 ng cDNA template, 1.5 μl <t>QuantiTect</t> Primer Assay (Qiagen), 7.5 μl QuantiTect SYBR green PCR kit (Qiagen), and 5 μl ddH2O. Primers for IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, IFN ‐γ, and TNF ‐α were obtained from Qiagen. Cycling conditions included a hot start activation (5 minutes, 95°C), 40 cycles of 10 seconds denaturation (95°C), and annealing and extension (30 seconds, 60°C). Amplicons were quantified with the comparative threshold cycle ( CT ) method; data acquisition was performed using the 7500 System SDS Software Version 2.0.5 (Applied Biosystems). Amplification specificity was checked using melting curve according to the manufacturer's instructions. All samples were measured in duplicate and respective results were averaged. A–P, In allograft skin (A–H) and muscle (I–P) no significant differences in relative gene expression of cytokines was observed between anti‐ IL ‐1β‐treatment groups 3 and 4 and group 2 controls. Expression levels of all markers were normalized to those of group 2
    Quantitect Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 419 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect reverse transcription kit/product/Qiagen
    Average 99 stars, based on 419 article reviews
    Price from $9.99 to $1999.99
    quantitect reverse transcription kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    Role of miR-146 family in up-regulation of MAT2A expression in TAMR-MCF-7 cells A. Down-regulation of miR-146a and miR-146b expression in TAMR-MCF-7 cells. miR-146a and miR-146b expression in MCF-7 and TAMR-MCF-7 cells were determined by using miScript PCR kit with miScript primers specific for mature miR-146a and miR-146b. Samples were normalized to small nRNA U6. B. Effects of miR-146a and miR-146b mimics on the miR-146a/b expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics for 36 h (120 p mole/well). miR-146a and miR-146b levels were determined by using miScript PCR kit with miScript primers specific for mature miR-146a and miR-146b. Samples were normalized to small nRNA U6. C. Effects of miR-146a/b mimic on NF-κB activity and MAT2A protein expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics (120 p mole/well) for 36 h and immunoblottings were performed. The data were confirmed by two independent experiments. D. Effects of miR-146b mimic on MAT2A mRNA expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics (120 p mole/well) for 36 h. MAT2A mRNA levels were determined by quantitative RT-PCR. Data represent mean ± SD with 3 different samples (significant versus control mimic miR-treated TAMR-MCF-7 cells, * P

    Journal: Oncotarget

    Article Title: Induction of methionine adenosyltransferase 2A in tamoxifen-resistant breast cancer cells

    doi: 10.18632/oncotarget.5298

    Figure Lengend Snippet: Role of miR-146 family in up-regulation of MAT2A expression in TAMR-MCF-7 cells A. Down-regulation of miR-146a and miR-146b expression in TAMR-MCF-7 cells. miR-146a and miR-146b expression in MCF-7 and TAMR-MCF-7 cells were determined by using miScript PCR kit with miScript primers specific for mature miR-146a and miR-146b. Samples were normalized to small nRNA U6. B. Effects of miR-146a and miR-146b mimics on the miR-146a/b expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics for 36 h (120 p mole/well). miR-146a and miR-146b levels were determined by using miScript PCR kit with miScript primers specific for mature miR-146a and miR-146b. Samples were normalized to small nRNA U6. C. Effects of miR-146a/b mimic on NF-κB activity and MAT2A protein expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics (120 p mole/well) for 36 h and immunoblottings were performed. The data were confirmed by two independent experiments. D. Effects of miR-146b mimic on MAT2A mRNA expression in TAMR-MCF-7 cells. TAMR-MCF-7 cells were cultured in 6 wells plate and then transfected with miR-146a or miR-146b mimics (120 p mole/well) for 36 h. MAT2A mRNA levels were determined by quantitative RT-PCR. Data represent mean ± SD with 3 different samples (significant versus control mimic miR-treated TAMR-MCF-7 cells, * P

    Article Snippet: Total RNA was isolated by using Trizol (Invitrogen, USA) and was then converted to cDNA by using the miScript reverse transcription kit (Qiagen, Valencia, CA). miScript primers specific for mature miRNAs were hsa-miR-146a (5′-CCTCTGAAATTCAGTTCTTCAG-3′) and hsa-miR-146b (5′-TGCCCTGTGGACTCAGTTCTGG-3′)(Bioneer, Eumsung, Korea).

    Techniques: Expressing, Polymerase Chain Reaction, Cell Culture, Transfection, Activity Assay, Quantitative RT-PCR

    Conditions of real‐time PCR . (A) Detection of mature mi RNA with deletion or addition in the 3′ end by real‐time RT ‐ PCR . A total of 1 × 10 5 copies of synthesized miR21, miR21 with deletion (miR21‐1), miR21 with addition of single nucleotide (miR21 + 1), and miR21 with addition of two nucleotides (miR21 + 2) in the 3′ end of mature mi RNA were examined with the miScript PCR system (miScript SYBR green real‐time PCR ) and stem‐loop real‐time RT ‐ PCR (Taqman micro RNA assay). Error bars indicate standard error for triplicate analyses. (B) Comparison of formalin‐fixed paraffin‐embedded ( FFPE ) and frozen samples by real‐time RT ‐ PCR . miR‐ BART s and miR‐ BHRF 1s were quantified by the miScript PCR system. FFPE and frozen samples were obtained from xenotropically inoculated lymphoma tissues in severe combined immunodeficiency mice. (C) Cycle thresholds (Ct) for miR16 and miR21. The copy numbers of two cellular mi RNA s, miR16 and miR21, were measured and plotted in 12 representative clinical samples using the miScript PCR system. Linear approximation line (broken line) and correlation coefficient (R 2 ) are indicated.

    Journal: Cancer Medicine

    Article Title: Next‐generation sequencing of miRNAs in clinical samples of Epstein–Barr virus‐associated B‐cell lymphomas

    doi: 10.1002/cam4.1006

    Figure Lengend Snippet: Conditions of real‐time PCR . (A) Detection of mature mi RNA with deletion or addition in the 3′ end by real‐time RT ‐ PCR . A total of 1 × 10 5 copies of synthesized miR21, miR21 with deletion (miR21‐1), miR21 with addition of single nucleotide (miR21 + 1), and miR21 with addition of two nucleotides (miR21 + 2) in the 3′ end of mature mi RNA were examined with the miScript PCR system (miScript SYBR green real‐time PCR ) and stem‐loop real‐time RT ‐ PCR (Taqman micro RNA assay). Error bars indicate standard error for triplicate analyses. (B) Comparison of formalin‐fixed paraffin‐embedded ( FFPE ) and frozen samples by real‐time RT ‐ PCR . miR‐ BART s and miR‐ BHRF 1s were quantified by the miScript PCR system. FFPE and frozen samples were obtained from xenotropically inoculated lymphoma tissues in severe combined immunodeficiency mice. (C) Cycle thresholds (Ct) for miR16 and miR21. The copy numbers of two cellular mi RNA s, miR16 and miR21, were measured and plotted in 12 representative clinical samples using the miScript PCR system. Linear approximation line (broken line) and correlation coefficient (R 2 ) are indicated.

    Article Snippet: Real‐time RT‐PCR for miRNA Total RNA (100 ng) was reverse‐transcribed using the miScript Reverse Transcription Kit from Qiagen (Valencia, CA).

    Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Synthesized, Polymerase Chain Reaction, SYBR Green Assay, Formalin-fixed Paraffin-Embedded, Mouse Assay

    RT q PCR analysis showing relative gene expression levels of IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, TNF ‐α, and IFN ‐γ in allograft skin (A – H) and muscle (I – P). For RT q PCR , a total volume of 15 μl per reaction was used containing 50 ng cDNA template, 1.5 μl QuantiTect Primer Assay (Qiagen), 7.5 μl QuantiTect SYBR green PCR kit (Qiagen), and 5 μl ddH2O. Primers for IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, IFN ‐γ, and TNF ‐α were obtained from Qiagen. Cycling conditions included a hot start activation (5 minutes, 95°C), 40 cycles of 10 seconds denaturation (95°C), and annealing and extension (30 seconds, 60°C). Amplicons were quantified with the comparative threshold cycle ( CT ) method; data acquisition was performed using the 7500 System SDS Software Version 2.0.5 (Applied Biosystems). Amplification specificity was checked using melting curve according to the manufacturer's instructions. All samples were measured in duplicate and respective results were averaged. A–P, In allograft skin (A–H) and muscle (I–P) no significant differences in relative gene expression of cytokines was observed between anti‐ IL ‐1β‐treatment groups 3 and 4 and group 2 controls. Expression levels of all markers were normalized to those of group 2

    Journal: American Journal of Transplantation

    Article Title: Subcutaneous administration of a neutralizing IL‐1β antibody prolongs limb allograft survival, et al. Subcutaneous administration of a neutralizing IL‐1β antibody prolongs limb allograft survival

    doi: 10.1111/ajt.14765

    Figure Lengend Snippet: RT q PCR analysis showing relative gene expression levels of IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, TNF ‐α, and IFN ‐γ in allograft skin (A – H) and muscle (I – P). For RT q PCR , a total volume of 15 μl per reaction was used containing 50 ng cDNA template, 1.5 μl QuantiTect Primer Assay (Qiagen), 7.5 μl QuantiTect SYBR green PCR kit (Qiagen), and 5 μl ddH2O. Primers for IL ‐1α, IL ‐1β, IL ‐2, IL ‐6, IL ‐10, IL ‐17A, IFN ‐γ, and TNF ‐α were obtained from Qiagen. Cycling conditions included a hot start activation (5 minutes, 95°C), 40 cycles of 10 seconds denaturation (95°C), and annealing and extension (30 seconds, 60°C). Amplicons were quantified with the comparative threshold cycle ( CT ) method; data acquisition was performed using the 7500 System SDS Software Version 2.0.5 (Applied Biosystems). Amplification specificity was checked using melting curve according to the manufacturer's instructions. All samples were measured in duplicate and respective results were averaged. A–P, In allograft skin (A–H) and muscle (I–P) no significant differences in relative gene expression of cytokines was observed between anti‐ IL ‐1β‐treatment groups 3 and 4 and group 2 controls. Expression levels of all markers were normalized to those of group 2

    Article Snippet: One micrograms of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen).

    Techniques: Polymerase Chain Reaction, Expressing, SYBR Green Assay, Activation Assay, Software, Amplification