quantitative real time polymerase chain reaction  (Roche)

 
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    Structured Review

    Roche quantitative real time polymerase chain reaction
    Exogenous BDNF increases BDNF/TrkB/SYN mRNA levels of Alzheimer's disease rats. BDNF (A), TrkB (B), and SYN (C) mRNA expression in the rat hippocampus of each group was examined using <t>quantitative</t> <t>polymerase</t> <t>chain</t> <t>reaction.</t> Data are expressed as the mean ± SD (one-way analysis of variance followed by the Bonferroni confidence interval adjustment test). *** P
    Quantitative Real Time Polymerase Chain Reaction, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time polymerase chain reaction/product/Roche
    Average 92 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    quantitative real time polymerase chain reaction - by Bioz Stars, 2020-07
    92/100 stars

    Images

    1) Product Images from "Exogenous brain-derived neurotrophic factor attenuates cognitive impairment induced by okadaic acid in a rat model of Alzheimer's disease"

    Article Title: Exogenous brain-derived neurotrophic factor attenuates cognitive impairment induced by okadaic acid in a rat model of Alzheimer's disease

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.241471

    Exogenous BDNF increases BDNF/TrkB/SYN mRNA levels of Alzheimer's disease rats. BDNF (A), TrkB (B), and SYN (C) mRNA expression in the rat hippocampus of each group was examined using quantitative polymerase chain reaction. Data are expressed as the mean ± SD (one-way analysis of variance followed by the Bonferroni confidence interval adjustment test). *** P
    Figure Legend Snippet: Exogenous BDNF increases BDNF/TrkB/SYN mRNA levels of Alzheimer's disease rats. BDNF (A), TrkB (B), and SYN (C) mRNA expression in the rat hippocampus of each group was examined using quantitative polymerase chain reaction. Data are expressed as the mean ± SD (one-way analysis of variance followed by the Bonferroni confidence interval adjustment test). *** P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    2) Product Images from "Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head"

    Article Title: Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head

    Journal: Chinese Medical Journal

    doi: 10.4103/0366-6999.160564

    Overall profiles of the glucocorticoid versus control transcriptomes of bone microvascular endothelial cells (BMECs). (a) Relative distribution of expressed lncRNAs (expressed probes/total probes) derived from each chromosome; (b) Relative chromosomal distribution of differentially regulated (up- or down-regulated probe number/total probes number) lncRNAs; (c) Annotation of genomic context of differentially expressed lncRNAs; (d) Validation of microarray results by quantitative real-time polymerase chain reaction.
    Figure Legend Snippet: Overall profiles of the glucocorticoid versus control transcriptomes of bone microvascular endothelial cells (BMECs). (a) Relative distribution of expressed lncRNAs (expressed probes/total probes) derived from each chromosome; (b) Relative chromosomal distribution of differentially regulated (up- or down-regulated probe number/total probes number) lncRNAs; (c) Annotation of genomic context of differentially expressed lncRNAs; (d) Validation of microarray results by quantitative real-time polymerase chain reaction.

    Techniques Used: Derivative Assay, Microarray, Real-time Polymerase Chain Reaction

    3) Product Images from "Role of mitogen-activated protein kinase family in serum-induced leukaemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells"

    Article Title: Role of mitogen-activated protein kinase family in serum-induced leukaemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells

    Journal: British Journal of Pharmacology

    doi: 10.1038/sj.bjp.0704797

    Quantitative real-time polymerase chain reaction (PCR)
    Figure Legend Snippet: Quantitative real-time polymerase chain reaction (PCR)

    Techniques Used: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Regional differences in hepatitis C treatment with peginterferon and ribavirin in Japan: a retrospective cohort study
    Article Snippet: .. Serum HCV RNA level was measured using quantitative real-time polymerase chain reaction (COBAS Ampliprep/COBAS TaqMan HCV test; F. Hoffmann-La Roche Ltd, Basel, Switzerland). ..

    Article Title: Exogenous brain-derived neurotrophic factor attenuates cognitive impairment induced by okadaic acid in a rat model of Alzheimer's disease
    Article Snippet: .. Quantitative real-time polymerase chain reaction The fresh hippocampal tissues were put in commercial RNA extraction reagent (Roche Applied Science, Indianapolis, IN, USA) for quantitative polymerase chain reaction (qPCR) analysis. .. First-strand cDNA was synthesized with the use of 1 μg of total RNA (Transcriptor First Strand cDNA Synthesis Kit, Roche Applied Science).

    Article Title: β-Cryptoxanthin modulates the response to phytosterols in post-menopausal women carrying NPC1L1 L272L and ABCG8 A632 V polymorphisms: an exploratory study
    Article Snippet: .. Quantitative real-time polymerase chain reaction was performed in a Light Cycler apparatus (Roche Diagnostics, Mannheim, Germany) using the LightCycler-FastStart DNA Master SYBR Green I Kit (Roche Diagnostics). .. Each reaction was performed in a final volume of 20 µL containing 2 µL of the cDNA product sample, 0.5 µM of each primer and 1 × reaction mix including FastStar DNA polymerase reaction buffer, deoxyribonucleotide triphosphates (dNTPs) and SYBR green.

    Article Title: No Impact of Hepatitis B Virus Infection on Early Mortality Among Human Immunodeficiency Virus–Infected Patients in Southern Africa
    Article Snippet: .. All patients were tested for the presence of chronic HBV infection, defined as a positive HBsAg rapid test (Determine®, Alere, Yavne, Israel), and HBV viral load (VL) was measured in HIV/HBV-coinfected individuals using quantitative real-time polymerase chain reaction (Roche, Indianapolis, Indiana) from plasma or dried blood spots [ ]. ..

    Article Title: Aging-associated oxidative stress inhibits liver progenitor cell activation in mice
    Article Snippet: .. Quantitative real-time polymerase chain reaction was performed using SYBR Green PCR mix (Roche, Basel, Switzerland) on an ABI Prism 7900HT (Applied Biosystems, Carlsbad, CA, US) as previously reported [ ]. β-actin was used as an internal control to normalize for difference in the amount of total RNA in each sample. .. Relative expression of genes was calculated and expressed as 2−ΔΔCT .

    Article Title: Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head
    Article Snippet: .. Quantitative real-time polymerase chain reaction To validate the microarray results, quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the Lightcycler-Faststart DNA master SYBR green I PCR kit (Roche, Basel, Switzerland) according to the manufacturer's specifications. .. The comparative threshold cycle (CT) method was used for the calculation of amplification fold.

    Article Title: Gene Expression Differences Between Offspring of Long-Lived Individuals and Controls in Candidate Longevity Regions: Evidence for PAPSS2 as a Longevity Gene
    Article Snippet: .. Gene expression for PAPSS2 was validated using quantitative real-time polymerase chain reaction (qRT-PCR). cDNA was synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer recommended protocol. .. Steady state mRNA levels of PAPSS2 were determined by two-step qRT-PCR using TaqMan Gene Expression Assay Hs00989928_m1 (Life Technologies, Grand Island, NY) on the LightCycler 480 (Roche).

    Article Title: Role of mitogen-activated protein kinase family in serum-induced leukaemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells
    Article Snippet: .. Quantitative real-time polymerase chain reaction (real-time PCR) was performed using the LightCycler (Roche Diagnostic, Mannheim, Germany) technology. .. Primers and probes, described in , were designed and synthesized by Nihon Gene Research Lab's (Sendai, Japan).

    RNA Extraction:

    Article Title: Exogenous brain-derived neurotrophic factor attenuates cognitive impairment induced by okadaic acid in a rat model of Alzheimer's disease
    Article Snippet: .. Quantitative real-time polymerase chain reaction The fresh hippocampal tissues were put in commercial RNA extraction reagent (Roche Applied Science, Indianapolis, IN, USA) for quantitative polymerase chain reaction (qPCR) analysis. .. First-strand cDNA was synthesized with the use of 1 μg of total RNA (Transcriptor First Strand cDNA Synthesis Kit, Roche Applied Science).

    Synthesized:

    Article Title: Gene Expression Differences Between Offspring of Long-Lived Individuals and Controls in Candidate Longevity Regions: Evidence for PAPSS2 as a Longevity Gene
    Article Snippet: .. Gene expression for PAPSS2 was validated using quantitative real-time polymerase chain reaction (qRT-PCR). cDNA was synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer recommended protocol. .. Steady state mRNA levels of PAPSS2 were determined by two-step qRT-PCR using TaqMan Gene Expression Assay Hs00989928_m1 (Life Technologies, Grand Island, NY) on the LightCycler 480 (Roche).

    Infection:

    Article Title: No Impact of Hepatitis B Virus Infection on Early Mortality Among Human Immunodeficiency Virus–Infected Patients in Southern Africa
    Article Snippet: .. All patients were tested for the presence of chronic HBV infection, defined as a positive HBsAg rapid test (Determine®, Alere, Yavne, Israel), and HBV viral load (VL) was measured in HIV/HBV-coinfected individuals using quantitative real-time polymerase chain reaction (Roche, Indianapolis, Indiana) from plasma or dried blood spots [ ]. ..

    SYBR Green Assay:

    Article Title: β-Cryptoxanthin modulates the response to phytosterols in post-menopausal women carrying NPC1L1 L272L and ABCG8 A632 V polymorphisms: an exploratory study
    Article Snippet: .. Quantitative real-time polymerase chain reaction was performed in a Light Cycler apparatus (Roche Diagnostics, Mannheim, Germany) using the LightCycler-FastStart DNA Master SYBR Green I Kit (Roche Diagnostics). .. Each reaction was performed in a final volume of 20 µL containing 2 µL of the cDNA product sample, 0.5 µM of each primer and 1 × reaction mix including FastStar DNA polymerase reaction buffer, deoxyribonucleotide triphosphates (dNTPs) and SYBR green.

    Article Title: Aging-associated oxidative stress inhibits liver progenitor cell activation in mice
    Article Snippet: .. Quantitative real-time polymerase chain reaction was performed using SYBR Green PCR mix (Roche, Basel, Switzerland) on an ABI Prism 7900HT (Applied Biosystems, Carlsbad, CA, US) as previously reported [ ]. β-actin was used as an internal control to normalize for difference in the amount of total RNA in each sample. .. Relative expression of genes was calculated and expressed as 2−ΔΔCT .

    Article Title: Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head
    Article Snippet: .. Quantitative real-time polymerase chain reaction To validate the microarray results, quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the Lightcycler-Faststart DNA master SYBR green I PCR kit (Roche, Basel, Switzerland) according to the manufacturer's specifications. .. The comparative threshold cycle (CT) method was used for the calculation of amplification fold.

    Microarray:

    Article Title: Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head
    Article Snippet: .. Quantitative real-time polymerase chain reaction To validate the microarray results, quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the Lightcycler-Faststart DNA master SYBR green I PCR kit (Roche, Basel, Switzerland) according to the manufacturer's specifications. .. The comparative threshold cycle (CT) method was used for the calculation of amplification fold.

    Quantitative RT-PCR:

    Article Title: Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head
    Article Snippet: .. Quantitative real-time polymerase chain reaction To validate the microarray results, quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the Lightcycler-Faststart DNA master SYBR green I PCR kit (Roche, Basel, Switzerland) according to the manufacturer's specifications. .. The comparative threshold cycle (CT) method was used for the calculation of amplification fold.

    Article Title: Gene Expression Differences Between Offspring of Long-Lived Individuals and Controls in Candidate Longevity Regions: Evidence for PAPSS2 as a Longevity Gene
    Article Snippet: .. Gene expression for PAPSS2 was validated using quantitative real-time polymerase chain reaction (qRT-PCR). cDNA was synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer recommended protocol. .. Steady state mRNA levels of PAPSS2 were determined by two-step qRT-PCR using TaqMan Gene Expression Assay Hs00989928_m1 (Life Technologies, Grand Island, NY) on the LightCycler 480 (Roche).

    Expressing:

    Article Title: Gene Expression Differences Between Offspring of Long-Lived Individuals and Controls in Candidate Longevity Regions: Evidence for PAPSS2 as a Longevity Gene
    Article Snippet: .. Gene expression for PAPSS2 was validated using quantitative real-time polymerase chain reaction (qRT-PCR). cDNA was synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer recommended protocol. .. Steady state mRNA levels of PAPSS2 were determined by two-step qRT-PCR using TaqMan Gene Expression Assay Hs00989928_m1 (Life Technologies, Grand Island, NY) on the LightCycler 480 (Roche).

    Polymerase Chain Reaction:

    Article Title: Aging-associated oxidative stress inhibits liver progenitor cell activation in mice
    Article Snippet: .. Quantitative real-time polymerase chain reaction was performed using SYBR Green PCR mix (Roche, Basel, Switzerland) on an ABI Prism 7900HT (Applied Biosystems, Carlsbad, CA, US) as previously reported [ ]. β-actin was used as an internal control to normalize for difference in the amount of total RNA in each sample. .. Relative expression of genes was calculated and expressed as 2−ΔΔCT .

    Article Title: Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head
    Article Snippet: .. Quantitative real-time polymerase chain reaction To validate the microarray results, quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the Lightcycler-Faststart DNA master SYBR green I PCR kit (Roche, Basel, Switzerland) according to the manufacturer's specifications. .. The comparative threshold cycle (CT) method was used for the calculation of amplification fold.

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    Roche quantitati
    Quantitati, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitati/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitati - by Bioz Stars, 2020-07
    85/100 stars
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