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TaKaRa quantitative real time pcr total rnas
Quantitative Real Time Pcr Total Rnas, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantitative real time pcr total rnas/product/TaKaRa
Average 90 stars, based on 5 article reviews
Price from $9.99 to $1999.99
quantitative real time pcr total rnas - by Bioz Stars, 2020-08
90/100 stars

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RNA Extraction:

Article Title: The circular RNA PVT1/miR-203/HOXD3 pathway promotes the progression of human hepatocellular carcinoma
Article Snippet: .. RNA extraction and quantitative real-time PCR Total RNAs were isolated with RNAiso Plus (Takara, China). .. Then complementary DNA was synthesized by PrimeScript™ RT Master Mix (Takara, China). qPCR was conducted using SYBR Green in StepOneTM Real-Time PCR system (Applied Biosystems).

Article Title: Foliar Spraying with Compound Amino Acid-Iron Fertilizer Increases Leaf Fresh Weight, Photosynthesis, and Fe-S Cluster Gene Expression in Peach (Prunus persica (L.) Batsch)
Article Snippet: .. RNA Extraction and Quantitative Real-Time PCR Total RNAs were extracted from the leaf samples using a MiniBEST Plant RNA Extraction Kit (TaKaRa, Dalian, China) and then were reverse transcribed into cDNA using a PrimeScript™ RT Reagent Kit (TaKaRa, Kyoto, Japan). qRT-PCR was carried out on a 7500 Real-Time PCR System (Applied Biosystems, New York, USA), using a SYBR Premix Ex Taq reaction kit (TaKaRa, Kyoto, Japan). ..

Article Title: LncRNA BDNF-AS is associated with the malignant status and regulates cell proliferation and apoptosis in osteosarcoma
Article Snippet: .. RNA extraction and quantitative real-time PCR Total RNAs were extracted from tissues or cells using RNAiso Plus (Takara, Dalian, China) following the manufacturer’s instructions. .. Real-time qPCR was performed at ABI 7500 system (Applied Systems, Foster City, CA, U.S.A.) using the SYBR Premix ExTaqII (Takara, Dalian, China) with the following primers.

Synthesized:

Article Title: Silencing of Rieske Iron-Sulfur Protein Impacts Upon the Development and Reproduction of Spodoptera exigua by Regulating ATP Synthesis
Article Snippet: .. Quantitative real-time PCR Total RNAs was extracted from S. exigua as described above. cDNAs were then synthesized for quantitative real-time PCR (qRT-PCR) using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) in accordance with the manufacturer's instructions. cDNAs were diluted 10 times to perform PCR for expression level analysis, or qRT-PCR for expression pattern analysis. .. The 25 μL reaction volume consisted of 1 μL of cDNA, 12.5 μL of SYBR Green (TaKaRa, Dalian, China), 10.5 μL of ddH2 O, 0.5 μL of forward primer (10 μM) and 0.5 μL of reverse primer (10 μM).

Isolation:

Article Title: The Rice Diacylglycerol Kinase Family: Functional Analysis Using Transient RNA Interference
Article Snippet: .. RNA isolation, RT-PCR, and real-time PCR Total RNAs were isolated from suspension cells or protoplasts using Trizol reagent according to the manufacturer’s protocol (Takara, Japan). .. Reverse transcription was performed using Prime Script™ RT Reagent Kit (Takara).

Article Title: Beneficial effects of early hemostasis on spinal cord injury in the rat
Article Snippet: .. Quantitative real-time PCR Total RNAs from 1 cm of the spinal cord segments with the lesion site approximately at the middle were isolated by RNAiso plus (Takara Bio Inc.) according to the manufacturer's instruction at 7 days, and 10 days after SCC. .. By using the SYBRGreen reaction kit, quantitative real-time PCR was performed (Takara Bio Inc., Kusatsu, Japan) with a Bio-Rad CFX 96 Real-Time System.

Article Title: The circular RNA PVT1/miR-203/HOXD3 pathway promotes the progression of human hepatocellular carcinoma
Article Snippet: .. RNA extraction and quantitative real-time PCR Total RNAs were isolated with RNAiso Plus (Takara, China). .. Then complementary DNA was synthesized by PrimeScript™ RT Master Mix (Takara, China). qPCR was conducted using SYBR Green in StepOneTM Real-Time PCR system (Applied Biosystems).

Article Title: P53 Regulation-Association Long Non-Coding RNA (LncRNA PRAL) Inhibits Cell Proliferation by Regulation of P53 in Human Lung Cancer
Article Snippet: .. RNA isolation and real-time polymerase chain reaction Total RNAs from clinical samples and cultured cells were extracted by TRIzol reagent (TaKaRa, Dalian, China) with a dose of 1 ml/well for 6-well plates and quantified by Nanodrop 2000 by measuring the absorbance of 260 nm and 280 nm. cDNAs were reversely transcribed by use of a synthesis kit (TaKaRa). .. RT-PCR was performed with the SYBR Premiers Ex Taq Kit (TaKaRa) in an ABI PRISM 7900 real-time system (ABI Co., NY, USA).

Quantitative RT-PCR:

Article Title: Silencing of Rieske Iron-Sulfur Protein Impacts Upon the Development and Reproduction of Spodoptera exigua by Regulating ATP Synthesis
Article Snippet: .. Quantitative real-time PCR Total RNAs was extracted from S. exigua as described above. cDNAs were then synthesized for quantitative real-time PCR (qRT-PCR) using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) in accordance with the manufacturer's instructions. cDNAs were diluted 10 times to perform PCR for expression level analysis, or qRT-PCR for expression pattern analysis. .. The 25 μL reaction volume consisted of 1 μL of cDNA, 12.5 μL of SYBR Green (TaKaRa, Dalian, China), 10.5 μL of ddH2 O, 0.5 μL of forward primer (10 μM) and 0.5 μL of reverse primer (10 μM).

Article Title: Foliar Spraying with Compound Amino Acid-Iron Fertilizer Increases Leaf Fresh Weight, Photosynthesis, and Fe-S Cluster Gene Expression in Peach (Prunus persica (L.) Batsch)
Article Snippet: .. RNA Extraction and Quantitative Real-Time PCR Total RNAs were extracted from the leaf samples using a MiniBEST Plant RNA Extraction Kit (TaKaRa, Dalian, China) and then were reverse transcribed into cDNA using a PrimeScript™ RT Reagent Kit (TaKaRa, Kyoto, Japan). qRT-PCR was carried out on a 7500 Real-Time PCR System (Applied Biosystems, New York, USA), using a SYBR Premix Ex Taq reaction kit (TaKaRa, Kyoto, Japan). ..

Real-time Polymerase Chain Reaction:

Article Title: The Rice Diacylglycerol Kinase Family: Functional Analysis Using Transient RNA Interference
Article Snippet: .. RNA isolation, RT-PCR, and real-time PCR Total RNAs were isolated from suspension cells or protoplasts using Trizol reagent according to the manufacturer’s protocol (Takara, Japan). .. Reverse transcription was performed using Prime Script™ RT Reagent Kit (Takara).

Article Title: Silencing of Rieske Iron-Sulfur Protein Impacts Upon the Development and Reproduction of Spodoptera exigua by Regulating ATP Synthesis
Article Snippet: .. Quantitative real-time PCR Total RNAs was extracted from S. exigua as described above. cDNAs were then synthesized for quantitative real-time PCR (qRT-PCR) using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) in accordance with the manufacturer's instructions. cDNAs were diluted 10 times to perform PCR for expression level analysis, or qRT-PCR for expression pattern analysis. .. The 25 μL reaction volume consisted of 1 μL of cDNA, 12.5 μL of SYBR Green (TaKaRa, Dalian, China), 10.5 μL of ddH2 O, 0.5 μL of forward primer (10 μM) and 0.5 μL of reverse primer (10 μM).

Article Title: Beneficial effects of early hemostasis on spinal cord injury in the rat
Article Snippet: .. Quantitative real-time PCR Total RNAs from 1 cm of the spinal cord segments with the lesion site approximately at the middle were isolated by RNAiso plus (Takara Bio Inc.) according to the manufacturer's instruction at 7 days, and 10 days after SCC. .. By using the SYBRGreen reaction kit, quantitative real-time PCR was performed (Takara Bio Inc., Kusatsu, Japan) with a Bio-Rad CFX 96 Real-Time System.

Article Title: The circular RNA PVT1/miR-203/HOXD3 pathway promotes the progression of human hepatocellular carcinoma
Article Snippet: .. RNA extraction and quantitative real-time PCR Total RNAs were isolated with RNAiso Plus (Takara, China). .. Then complementary DNA was synthesized by PrimeScript™ RT Master Mix (Takara, China). qPCR was conducted using SYBR Green in StepOneTM Real-Time PCR system (Applied Biosystems).

Article Title: Foliar Spraying with Compound Amino Acid-Iron Fertilizer Increases Leaf Fresh Weight, Photosynthesis, and Fe-S Cluster Gene Expression in Peach (Prunus persica (L.) Batsch)
Article Snippet: .. RNA Extraction and Quantitative Real-Time PCR Total RNAs were extracted from the leaf samples using a MiniBEST Plant RNA Extraction Kit (TaKaRa, Dalian, China) and then were reverse transcribed into cDNA using a PrimeScript™ RT Reagent Kit (TaKaRa, Kyoto, Japan). qRT-PCR was carried out on a 7500 Real-Time PCR System (Applied Biosystems, New York, USA), using a SYBR Premix Ex Taq reaction kit (TaKaRa, Kyoto, Japan). ..

Article Title: Activated CD4+ T cells-derived exosomal miR-142-3p boosts post-ischemic ventricular remodeling by activating myofibroblast
Article Snippet: .. Quantitative real-time PCR Total RNAs were extracted with TRIZOL reagent following the manufacturer's instructions (Takara, Dalian, China). .. The PrimeScriptTM RT reagent Kit (Takara Bio, Shiga, Japan), miRNA reverse transcription kit and miR-142-specific stem-loop primers (Sangon Biotech, shanghai, China) were used to perform the reverse transcription in order to synthesize single-stranded cDNA. qRT-PCR was performed with the SYBR Green PCR Master Mix (TaKaRa, China) using ABI-7300 Real-Time PCR Detection System (Applied Biosystems, USA).

Article Title: P53 Regulation-Association Long Non-Coding RNA (LncRNA PRAL) Inhibits Cell Proliferation by Regulation of P53 in Human Lung Cancer
Article Snippet: .. RNA isolation and real-time polymerase chain reaction Total RNAs from clinical samples and cultured cells were extracted by TRIzol reagent (TaKaRa, Dalian, China) with a dose of 1 ml/well for 6-well plates and quantified by Nanodrop 2000 by measuring the absorbance of 260 nm and 280 nm. cDNAs were reversely transcribed by use of a synthesis kit (TaKaRa). .. RT-PCR was performed with the SYBR Premiers Ex Taq Kit (TaKaRa) in an ABI PRISM 7900 real-time system (ABI Co., NY, USA).

Article Title: LncRNA BDNF-AS is associated with the malignant status and regulates cell proliferation and apoptosis in osteosarcoma
Article Snippet: .. RNA extraction and quantitative real-time PCR Total RNAs were extracted from tissues or cells using RNAiso Plus (Takara, Dalian, China) following the manufacturer’s instructions. .. Real-time qPCR was performed at ABI 7500 system (Applied Systems, Foster City, CA, U.S.A.) using the SYBR Premix ExTaqII (Takara, Dalian, China) with the following primers.

Reverse Transcription Polymerase Chain Reaction:

Article Title: The Rice Diacylglycerol Kinase Family: Functional Analysis Using Transient RNA Interference
Article Snippet: .. RNA isolation, RT-PCR, and real-time PCR Total RNAs were isolated from suspension cells or protoplasts using Trizol reagent according to the manufacturer’s protocol (Takara, Japan). .. Reverse transcription was performed using Prime Script™ RT Reagent Kit (Takara).

Cell Culture:

Article Title: P53 Regulation-Association Long Non-Coding RNA (LncRNA PRAL) Inhibits Cell Proliferation by Regulation of P53 in Human Lung Cancer
Article Snippet: .. RNA isolation and real-time polymerase chain reaction Total RNAs from clinical samples and cultured cells were extracted by TRIzol reagent (TaKaRa, Dalian, China) with a dose of 1 ml/well for 6-well plates and quantified by Nanodrop 2000 by measuring the absorbance of 260 nm and 280 nm. cDNAs were reversely transcribed by use of a synthesis kit (TaKaRa). .. RT-PCR was performed with the SYBR Premiers Ex Taq Kit (TaKaRa) in an ABI PRISM 7900 real-time system (ABI Co., NY, USA).

Expressing:

Article Title: Silencing of Rieske Iron-Sulfur Protein Impacts Upon the Development and Reproduction of Spodoptera exigua by Regulating ATP Synthesis
Article Snippet: .. Quantitative real-time PCR Total RNAs was extracted from S. exigua as described above. cDNAs were then synthesized for quantitative real-time PCR (qRT-PCR) using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) in accordance with the manufacturer's instructions. cDNAs were diluted 10 times to perform PCR for expression level analysis, or qRT-PCR for expression pattern analysis. .. The 25 μL reaction volume consisted of 1 μL of cDNA, 12.5 μL of SYBR Green (TaKaRa, Dalian, China), 10.5 μL of ddH2 O, 0.5 μL of forward primer (10 μM) and 0.5 μL of reverse primer (10 μM).

Polymerase Chain Reaction:

Article Title: Silencing of Rieske Iron-Sulfur Protein Impacts Upon the Development and Reproduction of Spodoptera exigua by Regulating ATP Synthesis
Article Snippet: .. Quantitative real-time PCR Total RNAs was extracted from S. exigua as described above. cDNAs were then synthesized for quantitative real-time PCR (qRT-PCR) using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) in accordance with the manufacturer's instructions. cDNAs were diluted 10 times to perform PCR for expression level analysis, or qRT-PCR for expression pattern analysis. .. The 25 μL reaction volume consisted of 1 μL of cDNA, 12.5 μL of SYBR Green (TaKaRa, Dalian, China), 10.5 μL of ddH2 O, 0.5 μL of forward primer (10 μM) and 0.5 μL of reverse primer (10 μM).

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  • 93
    TaKaRa real time quantitative polymerase chain reaction qrt pcr total rna
    LncRNA CASC2 inhibited hypoxia-induced cell proliferation in rat pulmonary arterial tissues. ( a ) The mRNA expression of PCNA was detected by <t>qRT-PCR</t> in rats′ PAs tissues. ( b , c ) The protein expression of PCNA was detected by western blot analysis in rat pulmonary arterial tissues. ## p
    Real Time Quantitative Polymerase Chain Reaction Qrt Pcr Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time quantitative polymerase chain reaction qrt pcr total rna/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    real time quantitative polymerase chain reaction qrt pcr total rna - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    TaKaRa quantitative real time pcr qrt pcr total rna
    mRNA levels of galectins and the receptors of Gal-9 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using <t>qRT-PCR.</t> ( a ) mRNA levels of Gal-1, Gal-3, Gal-8, and Gal-9; ( b ) mRNA levels of Tim-3, CD44, CD137, and PDI. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P
    Quantitative Real Time Pcr Qrt Pcr Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time pcr qrt pcr total rna/product/TaKaRa
    Average 93 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    quantitative real time pcr qrt pcr total rna - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    LncRNA CASC2 inhibited hypoxia-induced cell proliferation in rat pulmonary arterial tissues. ( a ) The mRNA expression of PCNA was detected by qRT-PCR in rats′ PAs tissues. ( b , c ) The protein expression of PCNA was detected by western blot analysis in rat pulmonary arterial tissues. ## p

    Journal: Respiratory Research

    Article Title: Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension

    doi: 10.1186/s12931-019-1018-x

    Figure Lengend Snippet: LncRNA CASC2 inhibited hypoxia-induced cell proliferation in rat pulmonary arterial tissues. ( a ) The mRNA expression of PCNA was detected by qRT-PCR in rats′ PAs tissues. ( b , c ) The protein expression of PCNA was detected by western blot analysis in rat pulmonary arterial tissues. ## p

    Article Snippet: Real time-quantitative polymerase chain reaction (qRT-PCR) Total RNA from PASMCs or rat pulmonary arterial tissues was extracted using the Trizol reagent and reversely transcribed into cDNA using Prime-Script RT-PCR kit (Takara, Dalian, China) following the manufacturer′s instructions.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Up-regulation of lncRNA CASC2 inhibited proliferation in hypoxia-induced PASMCs. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in PASMCs. ( b ) Cell number of PASMCs was counted by CyQuant cell proliferation assay kit. ( c ) The mRNA expression of PCNA was detected by qRT-PCR in PASMCs. ( d ) The protein expression of PCNA was detected by western blot analysis in PASMCs. ( e , f ) Cell proliferation status of PASMCs was assessed by EdU staining assay. ### p

    Journal: Respiratory Research

    Article Title: Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension

    doi: 10.1186/s12931-019-1018-x

    Figure Lengend Snippet: Up-regulation of lncRNA CASC2 inhibited proliferation in hypoxia-induced PASMCs. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in PASMCs. ( b ) Cell number of PASMCs was counted by CyQuant cell proliferation assay kit. ( c ) The mRNA expression of PCNA was detected by qRT-PCR in PASMCs. ( d ) The protein expression of PCNA was detected by western blot analysis in PASMCs. ( e , f ) Cell proliferation status of PASMCs was assessed by EdU staining assay. ### p

    Article Snippet: Real time-quantitative polymerase chain reaction (qRT-PCR) Total RNA from PASMCs or rat pulmonary arterial tissues was extracted using the Trizol reagent and reversely transcribed into cDNA using Prime-Script RT-PCR kit (Takara, Dalian, China) following the manufacturer′s instructions.

    Techniques: Expressing, Quantitative RT-PCR, CyQUANT Assay, Proliferation Assay, Western Blot, Staining

    LncRNA CASC2 was down-regulated in hypoxia-induced rats and reversed the increase of mPAP and progression of right ventricular hypertrophy caused by hypoxia. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in rat pulmonary arterial tissues. ( b ) Systemic blood pressure (SBP) was assessed in normoxia and hypoxia-induced rats. ( c ) Mean pulmonary arterial pressure (mPAP) was detected in normoxia and hypoxia-induced rats. ( d ) The right ventricular hypertrophy index (RVI) was calculated in normoxia and hypoxia-induced rats. # p

    Journal: Respiratory Research

    Article Title: Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension

    doi: 10.1186/s12931-019-1018-x

    Figure Lengend Snippet: LncRNA CASC2 was down-regulated in hypoxia-induced rats and reversed the increase of mPAP and progression of right ventricular hypertrophy caused by hypoxia. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in rat pulmonary arterial tissues. ( b ) Systemic blood pressure (SBP) was assessed in normoxia and hypoxia-induced rats. ( c ) Mean pulmonary arterial pressure (mPAP) was detected in normoxia and hypoxia-induced rats. ( d ) The right ventricular hypertrophy index (RVI) was calculated in normoxia and hypoxia-induced rats. # p

    Article Snippet: Real time-quantitative polymerase chain reaction (qRT-PCR) Total RNA from PASMCs or rat pulmonary arterial tissues was extracted using the Trizol reagent and reversely transcribed into cDNA using Prime-Script RT-PCR kit (Takara, Dalian, China) following the manufacturer′s instructions.

    Techniques: Expressing, Quantitative RT-PCR

    mRNA levels of galectins and the receptors of Gal-9 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. ( a ) mRNA levels of Gal-1, Gal-3, Gal-8, and Gal-9; ( b ) mRNA levels of Tim-3, CD44, CD137, and PDI. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Journal: Scientific Reports

    Article Title: Blockage of Galectin-receptor Interactions by α-lactose Exacerbates Plasmodium berghei-induced Pulmonary Immunopathology

    doi: 10.1038/srep32024

    Figure Lengend Snippet: mRNA levels of galectins and the receptors of Gal-9 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. ( a ) mRNA levels of Gal-1, Gal-3, Gal-8, and Gal-9; ( b ) mRNA levels of Tim-3, CD44, CD137, and PDI. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Article Snippet: Measurement of mRNA expression using quantitative real-time PCR (qRT-PCR) Total RNA was extracted from about 100 mg of mouse lung and MLN tissues or macrophages of each group using a RNA Extraction Kit (TaKaRa Bio, Inc., Tokyo, Japan) according to the manufacturer’s protocol.

    Techniques: Infection, Mouse Assay, Quantitative RT-PCR

    mRNA levels of IFN-α, IFN-β, IFN-γ, IL-4, and IL-10 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Journal: Scientific Reports

    Article Title: Blockage of Galectin-receptor Interactions by α-lactose Exacerbates Plasmodium berghei-induced Pulmonary Immunopathology

    doi: 10.1038/srep32024

    Figure Lengend Snippet: mRNA levels of IFN-α, IFN-β, IFN-γ, IL-4, and IL-10 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Article Snippet: Measurement of mRNA expression using quantitative real-time PCR (qRT-PCR) Total RNA was extracted from about 100 mg of mouse lung and MLN tissues or macrophages of each group using a RNA Extraction Kit (TaKaRa Bio, Inc., Tokyo, Japan) according to the manufacturer’s protocol.

    Techniques: Infection, Mouse Assay, Quantitative RT-PCR

    Comparisons of the protein and mRNA expression patterns of three representative DEPs at four artificial ageing stages (WH98, WH50, WH20, and WH01) by iTRAQ and qRT-PCR. Solid lines represent mRNA expression patterns, and dotted lines represent protein expression patterns.

    Journal: PLoS ONE

    Article Title: Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling

    doi: 10.1371/journal.pone.0162851

    Figure Lengend Snippet: Comparisons of the protein and mRNA expression patterns of three representative DEPs at four artificial ageing stages (WH98, WH50, WH20, and WH01) by iTRAQ and qRT-PCR. Solid lines represent mRNA expression patterns, and dotted lines represent protein expression patterns.

    Article Snippet: RNA isolation and quantitative real-time PCR (qRT-PCR) analysis Total RNA from wheat embryos of WH98, WH50, WH20, and WH01 were extracted by using RNAiso Plus reagent (Takara, Tokyo, Japan), and genomic DNA was removed by treating with DNase I (Takara) following the manufacturer’s protocol.

    Techniques: Expressing, Quantitative RT-PCR

    The effect of TMPyP4 on cells adhesion to extracellular matrix. ( A–D ) TMPyP4 increase cell adhesion to extracellular matrix. Indicated cell lines were used. ( E ) qRT-PCR showed the abundance of MUC5B mRNA in A549 cancer cell line (NC) and A549 cells transfected with siRNA targeting MUC5B (siRNA_1 and siRNA_2). ( F ) MUC5B knock-down suppress the adhesion of A549 cancer cells to extracellular matrix. MUC5B deficient cells showed no increase of cell adhesion upon TMPyP4 treatment. Values are average ± SD of three independent experiments.

    Journal: Scientific Reports

    Article Title: TMPyP4 promotes cancer cell migration at low doses, but induces cell death at high doses

    doi: 10.1038/srep26592

    Figure Lengend Snippet: The effect of TMPyP4 on cells adhesion to extracellular matrix. ( A–D ) TMPyP4 increase cell adhesion to extracellular matrix. Indicated cell lines were used. ( E ) qRT-PCR showed the abundance of MUC5B mRNA in A549 cancer cell line (NC) and A549 cells transfected with siRNA targeting MUC5B (siRNA_1 and siRNA_2). ( F ) MUC5B knock-down suppress the adhesion of A549 cancer cells to extracellular matrix. MUC5B deficient cells showed no increase of cell adhesion upon TMPyP4 treatment. Values are average ± SD of three independent experiments.

    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA of A549 cells treated with indicated siRNA was extracted by RNAiso Plus Reagent (Takara). cDNA was produced using Superscript III Reverse Transcriptase (Invitrogen) and random primers. qRT-PCR were performed using Fast SYBR Green PCR mastermix (Invitrogen) and specific primers (MUC5B: 5′-GCCTACGAGGACTTCAACGTC-3′, 5′-CCTTGATGACAACACGGGTGA-3′; β-actin: 5′-CATGTACGTTGCTATCCAGGC-3′, 5′-CTCCTTAATGTCACGCACGAT-3′).

    Techniques: Quantitative RT-PCR, Transfection