Structured Review

iNtRON Biotechnology quantitative real time pcr total rna
Expression of β-defensins in the lungs of WT and Nod1-dificient mice in fected with A. baumannii . <t>RNA</t> was obtained from the lungs of WT and Nod1-deficient mice (n=5–6 mice per group) infected with A. baumannii . The gene expressions of mBD-1, -2, -3, and -4 were examined by real-time <t>PCR</t> (A–D). Fold increase (arbitrary unit) was obtained by comparing each level in the lungs from infected to that in uninfected control lungs. * P
Quantitative Real Time Pcr Total Rna, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Nucleotide-binding oligomerization domain 1 is dispensable for host immune responses against pulmonary infection of Acinetobacter baumannii in mice"

Article Title: Nucleotide-binding oligomerization domain 1 is dispensable for host immune responses against pulmonary infection of Acinetobacter baumannii in mice

Journal: Laboratory Animal Research

doi: 10.5625/lar.2018.34.4.295

Expression of β-defensins in the lungs of WT and Nod1-dificient mice in fected with A. baumannii . RNA was obtained from the lungs of WT and Nod1-deficient mice (n=5–6 mice per group) infected with A. baumannii . The gene expressions of mBD-1, -2, -3, and -4 were examined by real-time PCR (A–D). Fold increase (arbitrary unit) was obtained by comparing each level in the lungs from infected to that in uninfected control lungs. * P
Figure Legend Snippet: Expression of β-defensins in the lungs of WT and Nod1-dificient mice in fected with A. baumannii . RNA was obtained from the lungs of WT and Nod1-deficient mice (n=5–6 mice per group) infected with A. baumannii . The gene expressions of mBD-1, -2, -3, and -4 were examined by real-time PCR (A–D). Fold increase (arbitrary unit) was obtained by comparing each level in the lungs from infected to that in uninfected control lungs. * P

Techniques Used: Expressing, Mouse Assay, Infection, Real-time Polymerase Chain Reaction

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RNA Extraction:

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Article Title: Anti-obesity effect of Solidago virgaurea var. gigantea extract through regulation of adipogenesis and lipogenesis pathways in high-fat diet-induced obese mice (C57BL/6N)
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Article Title: Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants
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Article Title: Anti-Obesity Effect of the Above-Ground Part of Valeriana dageletiana Nakai ex F. Maek Extract in High-Fat Diet-Induced Obese C57BL/6N Mice
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Article Title: Myosin heavy chain is stabilized by BCL-2 interacting cell death suppressor (BIS) in skeletal muscle
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Article Title: Zileuton, a 5-Lipoxygenase Inhibitor, Exerts Anti-Angiogenic Effect by Inducing Apoptosis of HUVEC via BK Channel Activation
Article Snippet: .. RNA Preparation and Real-Time PCR Total RNA was extracted from cells using easy-BLUE Total RNA extraction kit (Intron Biotechnology). ..

Synthesized:

Article Title: Myosin heavy chain is stabilized by BCL-2 interacting cell death suppressor (BIS) in skeletal muscle
Article Snippet: .. Quantitative real-time PCR Total RNA was extracted from cultured cells with a RNA extraction kit (iNtRON Biotechnology, Gyeonggi-do, Korea), and cDNA was synthesized using PrimeScript RTase (Clontech Takara Bio., Mountain View, CA, USA). .. Gene expression was measured according to standard quantitative PCR (qPCR) procedures with ToPreal qPCR 2X PreMIX (Enzynomics, Daejeon, Korea).

Isolation:

Article Title: Decreased Progesterone Receptor B/A Ratio in Endometrial Cells by Tumor Necrosis Factor-Alpha and Peritoneal Fluid from Patients with Endometriosis
Article Snippet: .. RNA isolation, reverse transcription, and real-time PCR Total RNA was extracted from the isolated cells using an Easy-blueTM Total RNA extraction kit (Intron Biotechnology, Seoul, Korea). .. RNA was measured using a Nanodrop instrument and stored at -80℃.

Article Title: Anti-obesity effect of Solidago virgaurea var. gigantea extract through regulation of adipogenesis and lipogenesis pathways in high-fat diet-induced obese mice (C57BL/6N)
Article Snippet: .. RNA extraction, cDNA synthesis, and real-time polymerase chain reaction Total RNA was isolated from the epididymal adipose tissue using an Easy-Blue kit (Intron Biotechnology, Seoul, Korea) according to the manufacturer’s instructions. .. Then, total RNA qualification was performed with a NanoDrop-2000 (Thermo Fisher Scientific; Wilmington, DE, USA).

Cell Culture:

Article Title: Myosin heavy chain is stabilized by BCL-2 interacting cell death suppressor (BIS) in skeletal muscle
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Real-time Polymerase Chain Reaction:

Article Title: Decreased Progesterone Receptor B/A Ratio in Endometrial Cells by Tumor Necrosis Factor-Alpha and Peritoneal Fluid from Patients with Endometriosis
Article Snippet: .. RNA isolation, reverse transcription, and real-time PCR Total RNA was extracted from the isolated cells using an Easy-blueTM Total RNA extraction kit (Intron Biotechnology, Seoul, Korea). .. RNA was measured using a Nanodrop instrument and stored at -80℃.

Article Title: Anti-obesity effect of Solidago virgaurea var. gigantea extract through regulation of adipogenesis and lipogenesis pathways in high-fat diet-induced obese mice (C57BL/6N)
Article Snippet: .. RNA extraction, cDNA synthesis, and real-time polymerase chain reaction Total RNA was isolated from the epididymal adipose tissue using an Easy-Blue kit (Intron Biotechnology, Seoul, Korea) according to the manufacturer’s instructions. .. Then, total RNA qualification was performed with a NanoDrop-2000 (Thermo Fisher Scientific; Wilmington, DE, USA).

Article Title: Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants
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Article Title: Nucleotide-binding oligomerization domain 1 is dispensable for host immune responses against pulmonary infection of Acinetobacter baumannii in mice
Article Snippet: .. Quantitative real-time PCR Total RNA was extracted from lung tissue using easy-BLUE (Intron Biotechnology, Korea) according to the manufacturer's instructions. .. One microgram of total RNA was reverse transcribed to cDNA by using ReverTra Ace qPCR RT Master Mix and cDNA Synthesis kit (Toyobo, Osaka, Japan).

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Article Title: Myosin heavy chain is stabilized by BCL-2 interacting cell death suppressor (BIS) in skeletal muscle
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Transgenic Assay:

Article Title: Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants
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Expressing:

Article Title: Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants
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    iNtRON Biotechnology total rna
    Regulation of expression of cytokines and iNOS by GE. <t>RAW</t> 264.7 cells were treated with the steam (a) or 50% ethanol (b) GE at various concentrations for 24 h. Total <t>RNA</t> was extracted and the expression was measured by real-time PCR. The data were expressed as mean ± SEM. ∗ p
    Total Rna, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 116 article reviews
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    iNtRON Biotechnology real time quantitative rt pcr qrt pcr analyses total rna
    Molecular characterization of OsDIRP1- overexpressing and RNAi -mediated knock-down transgenic rice plants. (A) Morphology of 2-month-old wild-type (WT), T4 Ubi:OsDIRP1-sGFP , and T4 Ubi:RNAi-OsDIRP1 rice plants grown under long-day conditions (16 h light and 8 h dark). (B) Genomic Southern blot analysis. Total leaf genomic DNA was isolated from wild-type (WT), T4 Ubi:OsDIRP1-sGFP (lines #1, #2, and #3), and T4 Ubi:RNAi-OsDIRP1 (lines #1, #2, and #3) rice plants. The DNA was digested with Hin dIII and hybridized to a 32 P-labeled hygromycin B phosphotransferase ( Hph ) probe under high stringency conditions. (C) <t>RT-PCR</t> analysis of the wild-type (WT) and T4 Ubi:OsDIRP1-sGFP (independent lines #1, #2, and #3) transgenic rice plants to examine OsDIRP1 transcript levels. OsUbiquitin was used as a loading control. (D) Immunoblot analysis of wild-type (WT) and T4 Ubi:OsDIRP1-sGFP plants. Total proteins were isolated using 2x SDS sample buffer and immunoblotted with anti-GFP antibody. Rubisco was used as an equal loading control. (E) RT-PCR analysis of the wild-type (WT) and T4 Ubi:RNAi-OsDIRP1 plants. <t>RNA</t> was isolated from whole seedlings of non-drought-treated (0 h) and drought-treated (4 h) wild-type (WT) and Ubi:RNAi-OsDIRP1 (lines #1, #2, and #3) plants. OsUbiquitin was used as a loading control.
    Real Time Quantitative Rt Pcr Qrt Pcr Analyses Total Rna, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time quantitative rt pcr qrt pcr analyses total rna/product/iNtRON Biotechnology
    Average 92 stars, based on 8 article reviews
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    iNtRON Biotechnology real time qrt pcr total rna
    Hh signaling, which is associated with lung cancer cell proliferation was inhibited by CDO depletion. A. Real-time <t>qRT-PCR</t> for the expression levels of CDO and Hh signaling components (SHH, PTCH1 and GLI1) in BEAS-2B and NSCLC cell lines (A549, H1299, H460 and H520). Each expression level was normalized to the level of 18S rRNA. The relative amount of each component in NSCLCs was determined as the amount of each in BEAS-2B was set to 1.0. B. The number of viable cells was determined by cell counting at indicated time in A549, H1299, H460 and H520 treated with or without 50 µM SANT1. C. Cell viability was assessed by MTT assay at indicated time in A549, H1299, H460 and H520 treated with or without 50 µM SANT1. The absorbance was measured at 595 nm. D. RT-PCR for CDO in A549, H1299 and H460 transfected with the scrambled siRNA or siCDO #1. E. Real-time qRT-PCR for Hh signaling components in A549, H1299 and H460 transfected with the scrambled siRNA or siCDO. The expression level of each component was normalized to the level of 18S rRNA. The relative amount of each component in CDO-depleted NSCLCs was determined as the amount of each in the control cells was set to 1.0 (red line). All the values represent means of at least triplicate determinations ±1 SD. * p
    Real Time Qrt Pcr Total Rna, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time qrt pcr total rna/product/iNtRON Biotechnology
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Regulation of expression of cytokines and iNOS by GE. RAW 264.7 cells were treated with the steam (a) or 50% ethanol (b) GE at various concentrations for 24 h. Total RNA was extracted and the expression was measured by real-time PCR. The data were expressed as mean ± SEM. ∗ p

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Immunomodulatory Efficacy of Standardized Annona muricata (Graviola) Leaf Extract via Activation of Mitogen-Activated Protein Kinase Pathways in RAW 264.7 Macrophages

    doi: 10.1155/2016/2905127

    Figure Lengend Snippet: Regulation of expression of cytokines and iNOS by GE. RAW 264.7 cells were treated with the steam (a) or 50% ethanol (b) GE at various concentrations for 24 h. Total RNA was extracted and the expression was measured by real-time PCR. The data were expressed as mean ± SEM. ∗ p

    Article Snippet: RNA Isolation and Real-Time RT-PCR RAW 264.7 cells were treated with 0, 50, 100, and 200 μ g/mL GE for 8 h after 8 × 105 cells/well were seeded into a 6-well plate and incubated for 24 h. Total RNA was isolated from RAW 264.7 cells using RNA Extraction Kit (Intron Biotechnology Inc., Korea) according to the manufacturer's instructions. cDNA was synthesized in a PCR Thermal Cycler (TaKaRa, Japan), using the iScript cDNA synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Expressing, Serial Time-encoded Amplified Microscopy, Real-time Polymerase Chain Reaction

    Molecular characterization of OsDIRP1- overexpressing and RNAi -mediated knock-down transgenic rice plants. (A) Morphology of 2-month-old wild-type (WT), T4 Ubi:OsDIRP1-sGFP , and T4 Ubi:RNAi-OsDIRP1 rice plants grown under long-day conditions (16 h light and 8 h dark). (B) Genomic Southern blot analysis. Total leaf genomic DNA was isolated from wild-type (WT), T4 Ubi:OsDIRP1-sGFP (lines #1, #2, and #3), and T4 Ubi:RNAi-OsDIRP1 (lines #1, #2, and #3) rice plants. The DNA was digested with Hin dIII and hybridized to a 32 P-labeled hygromycin B phosphotransferase ( Hph ) probe under high stringency conditions. (C) RT-PCR analysis of the wild-type (WT) and T4 Ubi:OsDIRP1-sGFP (independent lines #1, #2, and #3) transgenic rice plants to examine OsDIRP1 transcript levels. OsUbiquitin was used as a loading control. (D) Immunoblot analysis of wild-type (WT) and T4 Ubi:OsDIRP1-sGFP plants. Total proteins were isolated using 2x SDS sample buffer and immunoblotted with anti-GFP antibody. Rubisco was used as an equal loading control. (E) RT-PCR analysis of the wild-type (WT) and T4 Ubi:RNAi-OsDIRP1 plants. RNA was isolated from whole seedlings of non-drought-treated (0 h) and drought-treated (4 h) wild-type (WT) and Ubi:RNAi-OsDIRP1 (lines #1, #2, and #3) plants. OsUbiquitin was used as a loading control.

    Journal: Frontiers in Plant Science

    Article Title: OsDIRP1, a Putative RING E3 Ligase, Plays an Opposite Role in Drought and Cold Stress Responses as a Negative and Positive Factor, Respectively, in Rice (Oryza sativa L.)

    doi: 10.3389/fpls.2018.01797

    Figure Lengend Snippet: Molecular characterization of OsDIRP1- overexpressing and RNAi -mediated knock-down transgenic rice plants. (A) Morphology of 2-month-old wild-type (WT), T4 Ubi:OsDIRP1-sGFP , and T4 Ubi:RNAi-OsDIRP1 rice plants grown under long-day conditions (16 h light and 8 h dark). (B) Genomic Southern blot analysis. Total leaf genomic DNA was isolated from wild-type (WT), T4 Ubi:OsDIRP1-sGFP (lines #1, #2, and #3), and T4 Ubi:RNAi-OsDIRP1 (lines #1, #2, and #3) rice plants. The DNA was digested with Hin dIII and hybridized to a 32 P-labeled hygromycin B phosphotransferase ( Hph ) probe under high stringency conditions. (C) RT-PCR analysis of the wild-type (WT) and T4 Ubi:OsDIRP1-sGFP (independent lines #1, #2, and #3) transgenic rice plants to examine OsDIRP1 transcript levels. OsUbiquitin was used as a loading control. (D) Immunoblot analysis of wild-type (WT) and T4 Ubi:OsDIRP1-sGFP plants. Total proteins were isolated using 2x SDS sample buffer and immunoblotted with anti-GFP antibody. Rubisco was used as an equal loading control. (E) RT-PCR analysis of the wild-type (WT) and T4 Ubi:RNAi-OsDIRP1 plants. RNA was isolated from whole seedlings of non-drought-treated (0 h) and drought-treated (4 h) wild-type (WT) and Ubi:RNAi-OsDIRP1 (lines #1, #2, and #3) plants. OsUbiquitin was used as a loading control.

    Article Snippet: RNA Extraction, RT-PCR, and Real-Time Quantitative RT-PCR (qRT-PCR) Analyses Total RNA was extracted from various tissues of wild-type rice plants and stress-treated seedlings by using the Easy Spin Plants Total RNA Extraction kit (iNtRON Biotechnology, Korea) according to the manufacturer’s protocol.

    Techniques: Transgenic Assay, Southern Blot, Isolation, Labeling, Reverse Transcription Polymerase Chain Reaction

    Identification and characterization of OsDIRP1 in rice. (A) (Upper) Schematic diagram of full-length OsDIRP1 cDNA. The solid bar depicts the coding region. The solid lines represent the 5’- and 3’-untranslated regions. (Lower) Schematic structure of OsDIRP1. The putative beta-ketoacyl synthase active site, nuclear localization sequence (NLS), and RING motif are shown as dark gray bars. (B) RT-PCR analysis of OsDIRP1 in different tissues of rice plants. Total RNA was isolated from various rice tissues as indicated and analyzed by RT-PCR. OsUbiquitin was used as an equal loading control. (C) Expression patterns of OsDIRP1 in response to various abiotic stresses in rice plants. Light-grown, 10-day-old wild-type seedlings were subjected to drought, salt, cold, and ABA treatments at different time points as indicated. OsUbiquitin was used as an internal control for all the RT-PCR analyses. OsRab16b was used as a positive control for drought, salt, and ABA treatments, whereas OsDREB1A was used as a positive control for cold stress. (D) Subcellular localization of OsDIRP1. A 35S:OsDIRP1-sGFP fusion construct was transfected into wild-type rice protoplasts, and the fluorescent signals of the expressed proteins were visualized by fluorescence microscopy under dark-field conditions. sGFP and NLS-mRFP were used as cytosol- and nucleus-localized marker proteins, respectively. Bars = 5 μm.

    Journal: Frontiers in Plant Science

    Article Title: OsDIRP1, a Putative RING E3 Ligase, Plays an Opposite Role in Drought and Cold Stress Responses as a Negative and Positive Factor, Respectively, in Rice (Oryza sativa L.)

    doi: 10.3389/fpls.2018.01797

    Figure Lengend Snippet: Identification and characterization of OsDIRP1 in rice. (A) (Upper) Schematic diagram of full-length OsDIRP1 cDNA. The solid bar depicts the coding region. The solid lines represent the 5’- and 3’-untranslated regions. (Lower) Schematic structure of OsDIRP1. The putative beta-ketoacyl synthase active site, nuclear localization sequence (NLS), and RING motif are shown as dark gray bars. (B) RT-PCR analysis of OsDIRP1 in different tissues of rice plants. Total RNA was isolated from various rice tissues as indicated and analyzed by RT-PCR. OsUbiquitin was used as an equal loading control. (C) Expression patterns of OsDIRP1 in response to various abiotic stresses in rice plants. Light-grown, 10-day-old wild-type seedlings were subjected to drought, salt, cold, and ABA treatments at different time points as indicated. OsUbiquitin was used as an internal control for all the RT-PCR analyses. OsRab16b was used as a positive control for drought, salt, and ABA treatments, whereas OsDREB1A was used as a positive control for cold stress. (D) Subcellular localization of OsDIRP1. A 35S:OsDIRP1-sGFP fusion construct was transfected into wild-type rice protoplasts, and the fluorescent signals of the expressed proteins were visualized by fluorescence microscopy under dark-field conditions. sGFP and NLS-mRFP were used as cytosol- and nucleus-localized marker proteins, respectively. Bars = 5 μm.

    Article Snippet: RNA Extraction, RT-PCR, and Real-Time Quantitative RT-PCR (qRT-PCR) Analyses Total RNA was extracted from various tissues of wild-type rice plants and stress-treated seedlings by using the Easy Spin Plants Total RNA Extraction kit (iNtRON Biotechnology, Korea) according to the manufacturer’s protocol.

    Techniques: Sequencing, Reverse Transcription Polymerase Chain Reaction, Isolation, Expressing, Positive Control, Construct, Transfection, Fluorescence, Microscopy, Marker

    Expression analysis of cold stress-inducible genes in wild-type and OsDIRP1 -overexpressing transgenic rice plants. Light-grown, 10-day-old wild-type and Ubi:OsDIRP1-sGFP transgenic plants were exposed to cold (4°C) stress for 0 or 24 h. The induction patterns of five stress-responsive genes, OsDREB1A, OsDREB1B, OsDREB1D, GAD , and MRP4 , were analyzed by real-time qRT-PCR. The relative expression of each gene was normalized to that of OsActin . Data are means ± SE ( ∗ P

    Journal: Frontiers in Plant Science

    Article Title: OsDIRP1, a Putative RING E3 Ligase, Plays an Opposite Role in Drought and Cold Stress Responses as a Negative and Positive Factor, Respectively, in Rice (Oryza sativa L.)

    doi: 10.3389/fpls.2018.01797

    Figure Lengend Snippet: Expression analysis of cold stress-inducible genes in wild-type and OsDIRP1 -overexpressing transgenic rice plants. Light-grown, 10-day-old wild-type and Ubi:OsDIRP1-sGFP transgenic plants were exposed to cold (4°C) stress for 0 or 24 h. The induction patterns of five stress-responsive genes, OsDREB1A, OsDREB1B, OsDREB1D, GAD , and MRP4 , were analyzed by real-time qRT-PCR. The relative expression of each gene was normalized to that of OsActin . Data are means ± SE ( ∗ P

    Article Snippet: RNA Extraction, RT-PCR, and Real-Time Quantitative RT-PCR (qRT-PCR) Analyses Total RNA was extracted from various tissues of wild-type rice plants and stress-treated seedlings by using the Easy Spin Plants Total RNA Extraction kit (iNtRON Biotechnology, Korea) according to the manufacturer’s protocol.

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR

    M. paragordonae (Mpg) induced enhanced BMDC migration. Expression levels of CCR7 protein and mRNA were measured by ( a ) flow cytometry and ( b ) qRT-PCR, respectively. To show the rate of CCR7 + BMDCs, representative histograms of CD11c-gated cells are presented and the expression levels denote the relative fold changes based on β-actin (* P

    Journal: Scientific Reports

    Article Title: A temperature sensitive Mycobacterium paragordonae induces enhanced protective immune responses against mycobacterial infections in the mouse model

    doi: 10.1038/s41598-017-15458-7

    Figure Lengend Snippet: M. paragordonae (Mpg) induced enhanced BMDC migration. Expression levels of CCR7 protein and mRNA were measured by ( a ) flow cytometry and ( b ) qRT-PCR, respectively. To show the rate of CCR7 + BMDCs, representative histograms of CD11c-gated cells are presented and the expression levels denote the relative fold changes based on β-actin (* P

    Article Snippet: Quantitative real time-PCR (qRT-PCR) Total RNA from the BMDCs or THP-1 cells infected with BCG or Mpg was extracted using RNA-spinTM Total RNA Extraction kit (iNtRON Biotechnology, Gyeonggi-do, Korea) according to the manufacturer’s protocol.

    Techniques: Migration, Expressing, Flow Cytometry, Cytometry, Quantitative RT-PCR

    Hh signaling, which is associated with lung cancer cell proliferation was inhibited by CDO depletion. A. Real-time qRT-PCR for the expression levels of CDO and Hh signaling components (SHH, PTCH1 and GLI1) in BEAS-2B and NSCLC cell lines (A549, H1299, H460 and H520). Each expression level was normalized to the level of 18S rRNA. The relative amount of each component in NSCLCs was determined as the amount of each in BEAS-2B was set to 1.0. B. The number of viable cells was determined by cell counting at indicated time in A549, H1299, H460 and H520 treated with or without 50 µM SANT1. C. Cell viability was assessed by MTT assay at indicated time in A549, H1299, H460 and H520 treated with or without 50 µM SANT1. The absorbance was measured at 595 nm. D. RT-PCR for CDO in A549, H1299 and H460 transfected with the scrambled siRNA or siCDO #1. E. Real-time qRT-PCR for Hh signaling components in A549, H1299 and H460 transfected with the scrambled siRNA or siCDO. The expression level of each component was normalized to the level of 18S rRNA. The relative amount of each component in CDO-depleted NSCLCs was determined as the amount of each in the control cells was set to 1.0 (red line). All the values represent means of at least triplicate determinations ±1 SD. * p

    Journal: PLoS ONE

    Article Title: CDO, an Hh-Coreceptor, Mediates Lung Cancer Cell Proliferation and Tumorigenicity through Hedgehog Signaling

    doi: 10.1371/journal.pone.0111701

    Figure Lengend Snippet: Hh signaling, which is associated with lung cancer cell proliferation was inhibited by CDO depletion. A. Real-time qRT-PCR for the expression levels of CDO and Hh signaling components (SHH, PTCH1 and GLI1) in BEAS-2B and NSCLC cell lines (A549, H1299, H460 and H520). Each expression level was normalized to the level of 18S rRNA. The relative amount of each component in NSCLCs was determined as the amount of each in BEAS-2B was set to 1.0. B. The number of viable cells was determined by cell counting at indicated time in A549, H1299, H460 and H520 treated with or without 50 µM SANT1. C. Cell viability was assessed by MTT assay at indicated time in A549, H1299, H460 and H520 treated with or without 50 µM SANT1. The absorbance was measured at 595 nm. D. RT-PCR for CDO in A549, H1299 and H460 transfected with the scrambled siRNA or siCDO #1. E. Real-time qRT-PCR for Hh signaling components in A549, H1299 and H460 transfected with the scrambled siRNA or siCDO. The expression level of each component was normalized to the level of 18S rRNA. The relative amount of each component in CDO-depleted NSCLCs was determined as the amount of each in the control cells was set to 1.0 (red line). All the values represent means of at least triplicate determinations ±1 SD. * p

    Article Snippet: Total RNA preparation and real-time qRT-PCR Total RNA was isolated using easyBLUE total RNA extraction kit (iNtRON Biotechnology Inc., Korea), and cDNA was synthesized using PrimeScript RT reagent kit (TAKARA, Japan) according to manufacturer's instructions.

    Techniques: Quantitative RT-PCR, Expressing, Cell Counting, MTT Assay, Reverse Transcription Polymerase Chain Reaction, Transfection