quantitative polymerase chain reaction qpcr machine  (Stratagene)

 
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    Stratagene quantitative polymerase chain reaction qpcr machine
    Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative polymerase chain reaction qpcr machine/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative polymerase chain reaction qpcr machine - by Bioz Stars, 2020-09
    85/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Analysis of a DNA simulation model through hairpin melting experiments
    Article Snippet: .. A quantitative polymerase chain reaction (QPCR) machine (Mx3000P, Stratagene, La Jolla, California) was used to collect the temperature and fluorescence intensity data for the DNA hairpins in Table . .. Although acquiring melting curve data is not the standard use of a QPCR system, the machine’s accurate temperature control, ability to excite and detect fluorescent molecules, and its 96 sample capacity make it well suited for our experiments.

    Fluorescence:

    Article Title: Analysis of a DNA simulation model through hairpin melting experiments
    Article Snippet: .. A quantitative polymerase chain reaction (QPCR) machine (Mx3000P, Stratagene, La Jolla, California) was used to collect the temperature and fluorescence intensity data for the DNA hairpins in Table . .. Although acquiring melting curve data is not the standard use of a QPCR system, the machine’s accurate temperature control, ability to excite and detect fluorescent molecules, and its 96 sample capacity make it well suited for our experiments.

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    Stratagene stratagene qpcr machines
    Amplification plots of the same samples (as in Fig. 9 above) run on a <t>Stratagene</t> Mx3005P real-time <t>qPCR</t> machine. Note the consistency in relative C T values between results from the two different machines referred to in Figure 9 and this Figure. This bodes very well for highly-optimized qPCR in general. ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit was used.
    Stratagene Qpcr Machines, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stratagene qpcr machines/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stratagene qpcr machines - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    89
    Stratagene qrt pcr machine
    Stationary phase-specific pyruvate decarboxylases are regulated by Phx1 ( A ) The phylogenetic relatedness of various fungal PDC proteins. Amino acid sequences were aligned with ClustalW program, and a phylogenetic tree was constructed using the Neighbor-Joining method in MEGA 5 program. A Bootstrap test was performed for 1000 replicates and the values were indicated at each node. ( B ) Expression levels of pdc101 + , pdc102 + , pdc201 + , and pdc202 + genes in the wild type (JH43) and Δphx1 mutant (ESX5) at two growth phases. RNA samples were obtained from cells grown in EMM for 18 and 50 h for exponential and stationary phase cultures, respectively. The amounts of gene-specific mRNAs were estimated by <t>qRT-PCR,</t> along with that of act1 + mRNA as an internal control. Relative expression values to act1 + mRNA were obtained from three independent experiments, and were presented as an average with standard deviations. ( C ) Phx1-dependent PDC enzyme activity. Cell extracts were obtained from cells as described in ( B ). Pyruvate decarboxylase activity was measured as described in the text. Average values from three independent experiments were presented with standard deviations. ( D ) Effect of TPP addition on PDC activity. Experiments were done as in ( C ), except that TPP was added at 100 μM (final) to cell extracts.
    Qrt Pcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr machine/product/Stratagene
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qrt pcr machine - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    85
    Stratagene mx3005p real time qpcr machine
    Amplification plots of the same samples (as in Fig. 9 above) run on a Stratagene <t>Mx3005P</t> real-time <t>qPCR</t> machine. Note the consistency in relative C T values between results from the two different machines referred to in Figure 9 and this Figure. This bodes very well for highly-optimized qPCR in general. ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit was used.
    Mx3005p Real Time Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mx3005p real time qpcr machine/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mx3005p real time qpcr machine - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    88
    Stratagene mx4000 qpcr machine
    KLC1 is up-regulated in Loa . A , RNA was extracted using a NucleoSpin II RNA extraction kit from at least three E13 brains from each genotype. cDNA was generated with the Promega reverse transcription system, SYBR Green master mix, and a Stratagene <t>Mx4000</t> <t>qPCR</t> system was used for analysis. qPCR analysis revealed up-regulation of KLC1 in +/ Loa ( p = 0.006) and Loa / Loa ( p
    Mx4000 Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mx4000 qpcr machine/product/Stratagene
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mx4000 qpcr machine - by Bioz Stars, 2020-09
    88/100 stars
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    Amplification plots of the same samples (as in Fig. 9 above) run on a Stratagene Mx3005P real-time qPCR machine. Note the consistency in relative C T values between results from the two different machines referred to in Figure 9 and this Figure. This bodes very well for highly-optimized qPCR in general. ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit was used.

    Journal: Biological Procedures Online

    Article Title: Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

    doi: 10.1251/bpo122

    Figure Lengend Snippet: Amplification plots of the same samples (as in Fig. 9 above) run on a Stratagene Mx3005P real-time qPCR machine. Note the consistency in relative C T values between results from the two different machines referred to in Figure 9 and this Figure. This bodes very well for highly-optimized qPCR in general. ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit was used.

    Article Snippet: Since ABI master mixes contain 300 nM ROX, while Stratagene master mixes are formulated to contain 30 nM ROX (when ROX is used), it is important to note that investigators using ABI master mixes on Stratagene qPCR machines (Mx4000, Mx3000 and Mx3005P) cannot hope to use ROX as an agent for normalizing reporter fluorescence because the ABI ROX signal is simply too strong.

    Techniques: Amplification, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    Stationary phase-specific pyruvate decarboxylases are regulated by Phx1 ( A ) The phylogenetic relatedness of various fungal PDC proteins. Amino acid sequences were aligned with ClustalW program, and a phylogenetic tree was constructed using the Neighbor-Joining method in MEGA 5 program. A Bootstrap test was performed for 1000 replicates and the values were indicated at each node. ( B ) Expression levels of pdc101 + , pdc102 + , pdc201 + , and pdc202 + genes in the wild type (JH43) and Δphx1 mutant (ESX5) at two growth phases. RNA samples were obtained from cells grown in EMM for 18 and 50 h for exponential and stationary phase cultures, respectively. The amounts of gene-specific mRNAs were estimated by qRT-PCR, along with that of act1 + mRNA as an internal control. Relative expression values to act1 + mRNA were obtained from three independent experiments, and were presented as an average with standard deviations. ( C ) Phx1-dependent PDC enzyme activity. Cell extracts were obtained from cells as described in ( B ). Pyruvate decarboxylase activity was measured as described in the text. Average values from three independent experiments were presented with standard deviations. ( D ) Effect of TPP addition on PDC activity. Experiments were done as in ( C ), except that TPP was added at 100 μM (final) to cell extracts.

    Journal: Aging (Albany NY)

    Article Title: A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast

    doi:

    Figure Lengend Snippet: Stationary phase-specific pyruvate decarboxylases are regulated by Phx1 ( A ) The phylogenetic relatedness of various fungal PDC proteins. Amino acid sequences were aligned with ClustalW program, and a phylogenetic tree was constructed using the Neighbor-Joining method in MEGA 5 program. A Bootstrap test was performed for 1000 replicates and the values were indicated at each node. ( B ) Expression levels of pdc101 + , pdc102 + , pdc201 + , and pdc202 + genes in the wild type (JH43) and Δphx1 mutant (ESX5) at two growth phases. RNA samples were obtained from cells grown in EMM for 18 and 50 h for exponential and stationary phase cultures, respectively. The amounts of gene-specific mRNAs were estimated by qRT-PCR, along with that of act1 + mRNA as an internal control. Relative expression values to act1 + mRNA were obtained from three independent experiments, and were presented as an average with standard deviations. ( C ) Phx1-dependent PDC enzyme activity. Cell extracts were obtained from cells as described in ( B ). Pyruvate decarboxylase activity was measured as described in the text. Average values from three independent experiments were presented with standard deviations. ( D ) Effect of TPP addition on PDC activity. Experiments were done as in ( C ), except that TPP was added at 100 μM (final) to cell extracts.

    Article Snippet: Triplicate PCRs for gene-specific primer pairs of each gene were carried out according to manufacturer's instruction in a qRT-PCR machine(Stratagene MX3000P QPCR system, Agilent Technologies) with analysis software MXpro (Agilent Technologies).

    Techniques: Construct, Expressing, Mutagenesis, Quantitative RT-PCR, Activity Assay

    Thiamine supply is activated by Phx1 ( A ) The mRNA levels of genes involved in thiamine biosynthesis ( nmt1 + , nmt2 + ), transport ( bsu1 + , thi9 + ), and metabolism ( tnr3 + ) in wild type (WT; JH43) and Δphx1 (ESX5) mutant. Cells were grown in minimal media to either exponential or stationary phases, with (A2) or without (A1) adding 10 μM thiamine. The gene-specific mRNA levels were measured by qRT-PCR, along with that of act1 + mRNA as an internal control. Each internally normalized expression level at stationary phase was presented in the figure as a relative value to the level in exponential cells. Average values from three independent experiments were presented with standard deviations. ( B, C ) Intracellular levels of total thiamine pool ( B ) and TPP ( C ). Wild-type and Δphx1 mutant cells grown in minimal media were harvested at early exponential (12 h), late exponential (36 h), and late stationary (80 h) phases. Thiamine and thiamine phosphates (TMP, TPP) were extracted and measured by HPLC. Total thiamine is the sum of thiamine and thiamine phosphates. Average values with standard deviations (error bars) from at least three independent experiments were presented. Asterisks (**) represents p-value of

    Journal: Aging (Albany NY)

    Article Title: A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast

    doi:

    Figure Lengend Snippet: Thiamine supply is activated by Phx1 ( A ) The mRNA levels of genes involved in thiamine biosynthesis ( nmt1 + , nmt2 + ), transport ( bsu1 + , thi9 + ), and metabolism ( tnr3 + ) in wild type (WT; JH43) and Δphx1 (ESX5) mutant. Cells were grown in minimal media to either exponential or stationary phases, with (A2) or without (A1) adding 10 μM thiamine. The gene-specific mRNA levels were measured by qRT-PCR, along with that of act1 + mRNA as an internal control. Each internally normalized expression level at stationary phase was presented in the figure as a relative value to the level in exponential cells. Average values from three independent experiments were presented with standard deviations. ( B, C ) Intracellular levels of total thiamine pool ( B ) and TPP ( C ). Wild-type and Δphx1 mutant cells grown in minimal media were harvested at early exponential (12 h), late exponential (36 h), and late stationary (80 h) phases. Thiamine and thiamine phosphates (TMP, TPP) were extracted and measured by HPLC. Total thiamine is the sum of thiamine and thiamine phosphates. Average values with standard deviations (error bars) from at least three independent experiments were presented. Asterisks (**) represents p-value of

    Article Snippet: Triplicate PCRs for gene-specific primer pairs of each gene were carried out according to manufacturer's instruction in a qRT-PCR machine(Stratagene MX3000P QPCR system, Agilent Technologies) with analysis software MXpro (Agilent Technologies).

    Techniques: Mutagenesis, Quantitative RT-PCR, Expressing, High Performance Liquid Chromatography

    Amplification plots of the same samples (as in Fig. 9 above) run on a Stratagene Mx3005P real-time qPCR machine. Note the consistency in relative C T values between results from the two different machines referred to in Figure 9 and this Figure. This bodes very well for highly-optimized qPCR in general. ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit was used.

    Journal: Biological Procedures Online

    Article Title: Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

    doi: 10.1251/bpo122

    Figure Lengend Snippet: Amplification plots of the same samples (as in Fig. 9 above) run on a Stratagene Mx3005P real-time qPCR machine. Note the consistency in relative C T values between results from the two different machines referred to in Figure 9 and this Figure. This bodes very well for highly-optimized qPCR in general. ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit was used.

    Article Snippet: All reactions were run in an Applied Biosystems Incorporated (ABI) GeneAmp 5700® Sequence Detection System unless otherwise stated (in one case, a Stratagene Mx3005P real-time qPCR machine was employed – using ABI TaqMan® One-Step RT-PCR Master Mix Reagents Kit).

    Techniques: Amplification, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    KLC1 is up-regulated in Loa . A , RNA was extracted using a NucleoSpin II RNA extraction kit from at least three E13 brains from each genotype. cDNA was generated with the Promega reverse transcription system, SYBR Green master mix, and a Stratagene Mx4000 qPCR system was used for analysis. qPCR analysis revealed up-regulation of KLC1 in +/ Loa ( p = 0.006) and Loa / Loa ( p

    Journal: The Journal of Biological Chemistry

    Article Title: Neurodegenerative Mutation in Cytoplasmic Dynein Alters Its Organization and Dynein-Dynactin and Dynein-Kinesin Interactions *

    doi: 10.1074/jbc.M110.178087

    Figure Lengend Snippet: KLC1 is up-regulated in Loa . A , RNA was extracted using a NucleoSpin II RNA extraction kit from at least three E13 brains from each genotype. cDNA was generated with the Promega reverse transcription system, SYBR Green master mix, and a Stratagene Mx4000 qPCR system was used for analysis. qPCR analysis revealed up-regulation of KLC1 in +/ Loa ( p = 0.006) and Loa / Loa ( p

    Article Snippet: A Stratagene Mx4000 qPCR machine was set to denature at 95 °C for 15 min before 40 cycles of 94 °C denaturation for 30 s, annealing at 56 °C for 30 s, and elongation at 72 °C for 30 s. A dissociation curve followed each real time cycle.

    Techniques: RNA Extraction, Generated, SYBR Green Assay, Real-time Polymerase Chain Reaction