Journal: Biology Open
Article Title: Long non-coding RNA SNHG1 indicates poor prognosis and facilitates disease progression in acute myeloid leukemia
Figure Lengend Snippet: SNHG1 promotes the progression of AML by negatively regulating miR-101. (A) The relative expressions of miR-9, miR-22, miR-29b, miR-34a, miR-101, miR-126, miR-146a and miR-155 in MOLM-13 cells after knockdown of SNHG1 ( n =6). (B) Western blotting detecting the expressions of c-Fos, ZEB1 and Mcl-1 in MOLM-13 cells after knockdown of SNHG1. (C) Relative luciferase activity in HEK-293 cells after co-transfected with wild-type (wt) or mutant (mut) SNHG1 and miR-101 or control miRNA, determined by the luciferase reporter assay ( n =6). (D) qPCR analysis of the relative expression of miR-101 in BM specimens from 89 non-M3 AML patients and 27 healthy controls. (E) Pearson correlation analysis showing the significantly negative relationship between miR-101 and SNHG1 expression in specimens from 89 non-M3 AML patients (r=−0.4647, P
Article Snippet: For detection of miRNA, cDNA was synthesized by a miRcute Plus miRNA Fist-Strand cDNA Kit (TianGen Biotech, Beijing, China) and qPCR was conducted by a miRcute Plus miRNA qPCR Detection Kit (TianGen Biotech) according to the manufacturer's instructions.
Techniques: Western Blot, Luciferase, Activity Assay, Transfection, Mutagenesis, Reporter Assay, Real-time Polymerase Chain Reaction, Expressing