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tiangen biotech co quantitative polymerase chain reaction qpcr kit
Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantitative polymerase chain reaction qpcr kit/product/tiangen biotech co
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
quantitative polymerase chain reaction qpcr kit - by Bioz Stars, 2020-09
91/100 stars

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Article Title: Cardioprotective Effect of Danshensu against Ischemic/Reperfusion Injury via c-Subunit of ATP Synthase Inhibition
Article Snippet: .. Quantitative polymerase chain reaction (QPCR) kit was from Tiangen (Beijing, China). ..

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    tiangen biotech co mircute plus mirna qpcr detection kit
    SNHG1 promotes the progression of AML by negatively regulating miR-101. (A) The relative expressions of miR-9, miR-22, miR-29b, miR-34a, miR-101, miR-126, miR-146a and miR-155 in MOLM-13 cells after knockdown of SNHG1 ( n =6). (B) Western blotting detecting the expressions of c-Fos, ZEB1 and Mcl-1 in MOLM-13 cells after knockdown of SNHG1. (C) Relative luciferase activity in HEK-293 cells after co-transfected with wild-type (wt) or mutant (mut) SNHG1 and miR-101 or control <t>miRNA,</t> determined by the luciferase reporter assay ( n =6). (D) <t>qPCR</t> analysis of the relative expression of miR-101 in BM specimens from 89 non-M3 AML patients and 27 healthy controls. (E) Pearson correlation analysis showing the significantly negative relationship between miR-101 and SNHG1 expression in specimens from 89 non-M3 AML patients (r=−0.4647, P
    Mircute Plus Mirna Qpcr Detection Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mircute plus mirna qpcr detection kit/product/tiangen biotech co
    Average 93 stars, based on 295 article reviews
    Price from $9.99 to $1999.99
    mircute plus mirna qpcr detection kit - by Bioz Stars, 2020-09
    93/100 stars
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    SNHG1 promotes the progression of AML by negatively regulating miR-101. (A) The relative expressions of miR-9, miR-22, miR-29b, miR-34a, miR-101, miR-126, miR-146a and miR-155 in MOLM-13 cells after knockdown of SNHG1 ( n =6). (B) Western blotting detecting the expressions of c-Fos, ZEB1 and Mcl-1 in MOLM-13 cells after knockdown of SNHG1. (C) Relative luciferase activity in HEK-293 cells after co-transfected with wild-type (wt) or mutant (mut) SNHG1 and miR-101 or control miRNA, determined by the luciferase reporter assay ( n =6). (D) qPCR analysis of the relative expression of miR-101 in BM specimens from 89 non-M3 AML patients and 27 healthy controls. (E) Pearson correlation analysis showing the significantly negative relationship between miR-101 and SNHG1 expression in specimens from 89 non-M3 AML patients (r=−0.4647, P

    Journal: Biology Open

    Article Title: Long non-coding RNA SNHG1 indicates poor prognosis and facilitates disease progression in acute myeloid leukemia

    doi: 10.1242/bio.046417

    Figure Lengend Snippet: SNHG1 promotes the progression of AML by negatively regulating miR-101. (A) The relative expressions of miR-9, miR-22, miR-29b, miR-34a, miR-101, miR-126, miR-146a and miR-155 in MOLM-13 cells after knockdown of SNHG1 ( n =6). (B) Western blotting detecting the expressions of c-Fos, ZEB1 and Mcl-1 in MOLM-13 cells after knockdown of SNHG1. (C) Relative luciferase activity in HEK-293 cells after co-transfected with wild-type (wt) or mutant (mut) SNHG1 and miR-101 or control miRNA, determined by the luciferase reporter assay ( n =6). (D) qPCR analysis of the relative expression of miR-101 in BM specimens from 89 non-M3 AML patients and 27 healthy controls. (E) Pearson correlation analysis showing the significantly negative relationship between miR-101 and SNHG1 expression in specimens from 89 non-M3 AML patients (r=−0.4647, P

    Article Snippet: For detection of miRNA, cDNA was synthesized by a miRcute Plus miRNA Fist-Strand cDNA Kit (TianGen Biotech, Beijing, China) and qPCR was conducted by a miRcute Plus miRNA qPCR Detection Kit (TianGen Biotech) according to the manufacturer's instructions.

    Techniques: Western Blot, Luciferase, Activity Assay, Transfection, Mutagenesis, Reporter Assay, Real-time Polymerase Chain Reaction, Expressing

    Validation for aberrant miRNA expression using qPCR assay. Total RNA including miRNAs were extracted from HBE and HBE-T cells and the miRNA level (miR-33b-5p, miR-15b-5p, miR-192-5p, miR-141-3p, miR-106b-5p, miR-200b-3p) was detected using qPCR assay. Level of miRNAs in HBE-T cells was normalized to corresponding miRNA level in HBE cells. Data were obtained from three independent experiments and quantitative results are presented as mean ± standard deviation (SD). p -Values were calculated using independent samples t test and * p

    Journal: Genes

    Article Title: Alterations of miRNAs and Their Potential Roles in Arsenite-Induced Transformation of Human Bronchial Epithelial Cells

    doi: 10.3390/genes8100254

    Figure Lengend Snippet: Validation for aberrant miRNA expression using qPCR assay. Total RNA including miRNAs were extracted from HBE and HBE-T cells and the miRNA level (miR-33b-5p, miR-15b-5p, miR-192-5p, miR-141-3p, miR-106b-5p, miR-200b-3p) was detected using qPCR assay. Level of miRNAs in HBE-T cells was normalized to corresponding miRNA level in HBE cells. Data were obtained from three independent experiments and quantitative results are presented as mean ± standard deviation (SD). p -Values were calculated using independent samples t test and * p

    Article Snippet: Then, an equal amount of each complementary DNA sample was subjected to fluorescence quantitative real-time polymerase chain reaction (qPCR) analysis on Bio-Rad using a miRcute miRNA qPCR Detection Kit (Tiangen Biotech, Beijing Co., Ltd).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation

    qPCR detection and Cell proliferation assay in TG PFF clones. ( A ) Relative expression levels of miRNAs expressed from the p miR-17-92 cluster in positive PFF clones #31 and #67 were confirmed by qPCR. All values are the mean ± S.E.M., n = 3. No significant difference was found among the groups. ( B ) The effect of the anti-CSFV shRNA insertion on cell proliferation in positive PFF clones. All values are the mean ± S.E.M., n = 3. No significant difference was found among the groups.

    Journal: Cells

    Article Title: CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

    doi: 10.3390/cells8020113

    Figure Lengend Snippet: qPCR detection and Cell proliferation assay in TG PFF clones. ( A ) Relative expression levels of miRNAs expressed from the p miR-17-92 cluster in positive PFF clones #31 and #67 were confirmed by qPCR. All values are the mean ± S.E.M., n = 3. No significant difference was found among the groups. ( B ) The effect of the anti-CSFV shRNA insertion on cell proliferation in positive PFF clones. All values are the mean ± S.E.M., n = 3. No significant difference was found among the groups.

    Article Snippet: Quantitative real time PCR (qPCR) was also performed using a miRcute miRNA qPCR Detection Kit (Tiangen, Beijing, China) according to the manufacturer′s instructions.

    Techniques: Real-time Polymerase Chain Reaction, Proliferation Assay, Clone Assay, Expressing, shRNA

    Genomic organization and expression of the p miR-17-92 cluster. ( A ) Schematic representation of the p miR-17-92 cluster. The p miR-17-92 cluster is located in an intergenic region on chromosome 11 in the porcine genome. Pre-miRNAs are indicated as color-coded boxes. Black boxes correspond to the mature miRNA. ( B ) Sequence comparison of the miRNAs expressed from the p miR-17-92 cluster. The seed sequences are indicated in red. ( C ) Polymorphism analysis of the p miR-17-92 cluster in different pig breeds and in two different cell lines. M: DNA marker III. PFFs: porcine fetal fibroblasts from Yorkshire (China) pigs. ( D ) Relative expression of pri-miR-17-92 in different adult porcine tissues as determined by real-time PCR. All values are the mean ± S.E.M., n = 3. ( E) Relative expression of miRNAs expressed from the p miR-17-92 cluster in a variety of tissues as determined by qPCR. All values are the mean ± S.E.M., n = 3.

    Journal: Cells

    Article Title: CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

    doi: 10.3390/cells8020113

    Figure Lengend Snippet: Genomic organization and expression of the p miR-17-92 cluster. ( A ) Schematic representation of the p miR-17-92 cluster. The p miR-17-92 cluster is located in an intergenic region on chromosome 11 in the porcine genome. Pre-miRNAs are indicated as color-coded boxes. Black boxes correspond to the mature miRNA. ( B ) Sequence comparison of the miRNAs expressed from the p miR-17-92 cluster. The seed sequences are indicated in red. ( C ) Polymorphism analysis of the p miR-17-92 cluster in different pig breeds and in two different cell lines. M: DNA marker III. PFFs: porcine fetal fibroblasts from Yorkshire (China) pigs. ( D ) Relative expression of pri-miR-17-92 in different adult porcine tissues as determined by real-time PCR. All values are the mean ± S.E.M., n = 3. ( E) Relative expression of miRNAs expressed from the p miR-17-92 cluster in a variety of tissues as determined by qPCR. All values are the mean ± S.E.M., n = 3.

    Article Snippet: Quantitative real time PCR (qPCR) was also performed using a miRcute miRNA qPCR Detection Kit (Tiangen, Beijing, China) according to the manufacturer′s instructions.

    Techniques: Expressing, Sequencing, Marker, Real-time Polymerase Chain Reaction

    miR-24 expression decreases and ATG4A increases following treatment with β-catenin inhibitor XAV-939 in glioma C6 cells transfected with miR-24. Glioma C6 cells transfected with miR-24 mimics, the negative control miRNA or either miRNA and the β-catenin inhibitor, XAV-939. The expression of miR-24 and mRNA expression of ATG4A were detected by reverse transcription-quantitative polymerase chain reaction analysis. Data are presented as the mean ± standard error of the mean; n=3/group. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: β-catenin regulates effects of miR-24 on the viability and autophagy of glioma cells

    doi: 10.3892/etm.2019.7680

    Figure Lengend Snippet: miR-24 expression decreases and ATG4A increases following treatment with β-catenin inhibitor XAV-939 in glioma C6 cells transfected with miR-24. Glioma C6 cells transfected with miR-24 mimics, the negative control miRNA or either miRNA and the β-catenin inhibitor, XAV-939. The expression of miR-24 and mRNA expression of ATG4A were detected by reverse transcription-quantitative polymerase chain reaction analysis. Data are presented as the mean ± standard error of the mean; n=3/group. *P

    Article Snippet: The primer for U6 was F: 5-CTC GCT TCG GCA GCA CA-3; R: 5-AAC GCT TCA CGA ATT TGC GT-3. miRcute miRNA qPCR Detection kit (Tiangen Biotech Co., Ltd.) was used for qPCR analysis.

    Techniques: Expressing, Transfection, Negative Control, Real-time Polymerase Chain Reaction