quantifast sybr green pcr kit  (Qiagen)


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    Name:
    QuantiFast SYBR Green PCR Kit
    Description:
    For fast real time PCR and two step qRT PCR using SYBR Green Kit contents Qiagen QuantiFast SYBR Green PCR Kit 400 x 25L rxns SYBR Green I cDNA and DNA Sample Real time and two step RT PCR Reaction Q bond Technology For use in Gene Expression Analysis of cDNA Targets and Quantitative gDNA Analysis Delivers Fast and Specific Quantification of gDNA or cDNA Targets Includes 3 x 1 7mL 2x QuantiFast SYBR Green PCR Master Mix Contains ROX Dye 2 x 2mL RNase free Water Benefits Specific and sensitive detection of even low copy targets Optimized master mix for reliable results without optimization 5 minute enzyme activation and fast cycling conditions Accurate detection of a wide range of template amounts Universal protocol for all standard and fast cycl
    Catalog Number:
    204054
    Price:
    449
    Category:
    QuantiFast SYBR Green PCR Kit
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    Structured Review

    Qiagen quantifast sybr green pcr kit
    QuantiFast SYBR Green PCR Kit
    For fast real time PCR and two step qRT PCR using SYBR Green Kit contents Qiagen QuantiFast SYBR Green PCR Kit 400 x 25L rxns SYBR Green I cDNA and DNA Sample Real time and two step RT PCR Reaction Q bond Technology For use in Gene Expression Analysis of cDNA Targets and Quantitative gDNA Analysis Delivers Fast and Specific Quantification of gDNA or cDNA Targets Includes 3 x 1 7mL 2x QuantiFast SYBR Green PCR Master Mix Contains ROX Dye 2 x 2mL RNase free Water Benefits Specific and sensitive detection of even low copy targets Optimized master mix for reliable results without optimization 5 minute enzyme activation and fast cycling conditions Accurate detection of a wide range of template amounts Universal protocol for all standard and fast cycl
    https://www.bioz.com/result/quantifast sybr green pcr kit/product/Qiagen
    Average 99 stars, based on 389 article reviews
    Price from $9.99 to $1999.99
    quantifast sybr green pcr kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The influence of neuropathic pain on nAChR plasticity and behavioral responses to nicotine in rats
    Article Snippet: .. Total RNA was extracted and purified using RNeasy MiniKit (Qiagen, Valencia, CA). cDNA reverse transcription was performed using Quantitect Reverse Transcription Kit (Qiagen, Valencia, CA). mRNA expression levels were determined by qPCR using a QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA) according to manufacturer’s protocol on the iCycler IQ5 multicolor real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA). ..

    Article Title: CVID Enteropathy is Characterized by Exceeding Low Mucosal IgA Levels and Interferon-Driven Inflammation Possibly Related to the Presence of a Pathobiont
    Article Snippet: .. Quantitative Real-Time PCR was done for the samples using QuantiFast SYBR Green PCR Kit (Qiagen, cat# 204056). qRT PCR set up used: samples were heated to 95°C, followed by 40 cycles of 95°C for 10 sec and 60°C for 30 sec. House-keeping gene used for normalization (SRSF11) was selected according to the procedure described in previous publication [ ]. .. Before been collected, cells in each well were washed with PBS and then lysed with Lysis Buffer from Amplex® Red Cholesterol Assay Kit (Invitrogen, cat#A12216), sonicated on ice 2 times 10 s each.

    Amplification:

    Article Title: The influence of neuropathic pain on nAChR plasticity and behavioral responses to nicotine in rats
    Article Snippet: Total RNA was extracted and purified using RNeasy MiniKit (Qiagen, Valencia, CA). cDNA reverse transcription was performed using Quantitect Reverse Transcription Kit (Qiagen, Valencia, CA). mRNA expression levels were determined by qPCR using a QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA) according to manufacturer’s protocol on the iCycler IQ5 multicolor real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA). .. Amplification efficiency of each primer pair was determined based on the slope of standard curves generated by 1:10 serial dilutions of a cDNA sample pooled from each region examined ( ).

    Isolation:

    Article Title: Nucleolar Organization, Ribosomal DNA Array Stability, and Acrocentric Chromosome Integrity Are Linked to Telomere Function
    Article Snippet: qRT-PCR cDNA was collected from uninduced T19 control, 12-, 24-, 48-, and 72-hour dnTRF2-expressing cells, serum starved cells, and cells treated with Actinomycin D for 30 minutes and 3 hours. cDNA was isolated using Qiagen's FastLane Cell cDNA kit (Cat. 215011). .. One confluent T25 flask per sample was used and the manufacturer's protocol was followed. qRT-PCRs were performed using Qiagen's QuantiFast SYBR Green PCR kit (Cat 204052) for GAPDH (primers: 5′-CTCATGACCACAGTCCATGCC-3′ , 5′-GCCATCCACAGTCTTCTGGGT-3′ ) , 45S pre-ribosomal RNA (primers: 5′-CTCCGTTATGGTAGCGCTGC-3′ , 5′-GCGGAACCCTCGCTTCTC-3′ ), and 28S ribosomal RNA (primers: 5′-CGACGACCCATTCGAACGTCT-3′ , 5′-CTCTCCGGAATCGAACCCTGA-3′ ) .

    Size-exclusion Chromatography:

    Article Title: CVID Enteropathy is Characterized by Exceeding Low Mucosal IgA Levels and Interferon-Driven Inflammation Possibly Related to the Presence of a Pathobiont
    Article Snippet: .. Quantitative Real-Time PCR was done for the samples using QuantiFast SYBR Green PCR Kit (Qiagen, cat# 204056). qRT PCR set up used: samples were heated to 95°C, followed by 40 cycles of 95°C for 10 sec and 60°C for 30 sec. House-keeping gene used for normalization (SRSF11) was selected according to the procedure described in previous publication [ ]. .. Before been collected, cells in each well were washed with PBS and then lysed with Lysis Buffer from Amplex® Red Cholesterol Assay Kit (Invitrogen, cat#A12216), sonicated on ice 2 times 10 s each.

    Quantitative RT-PCR:

    Article Title: Nucleolar Organization, Ribosomal DNA Array Stability, and Acrocentric Chromosome Integrity Are Linked to Telomere Function
    Article Snippet: Paragraph title: qRT-PCR ... One confluent T25 flask per sample was used and the manufacturer's protocol was followed. qRT-PCRs were performed using Qiagen's QuantiFast SYBR Green PCR kit (Cat 204052) for GAPDH (primers: 5′-CTCATGACCACAGTCCATGCC-3′ , 5′-GCCATCCACAGTCTTCTGGGT-3′ ) , 45S pre-ribosomal RNA (primers: 5′-CTCCGTTATGGTAGCGCTGC-3′ , 5′-GCGGAACCCTCGCTTCTC-3′ ), and 28S ribosomal RNA (primers: 5′-CGACGACCCATTCGAACGTCT-3′ , 5′-CTCTCCGGAATCGAACCCTGA-3′ ) .

    Article Title: CVID Enteropathy is Characterized by Exceeding Low Mucosal IgA Levels and Interferon-Driven Inflammation Possibly Related to the Presence of a Pathobiont
    Article Snippet: .. Quantitative Real-Time PCR was done for the samples using QuantiFast SYBR Green PCR Kit (Qiagen, cat# 204056). qRT PCR set up used: samples were heated to 95°C, followed by 40 cycles of 95°C for 10 sec and 60°C for 30 sec. House-keeping gene used for normalization (SRSF11) was selected according to the procedure described in previous publication [ ]. .. Before been collected, cells in each well were washed with PBS and then lysed with Lysis Buffer from Amplex® Red Cholesterol Assay Kit (Invitrogen, cat#A12216), sonicated on ice 2 times 10 s each.

    Purification:

    Article Title: The influence of neuropathic pain on nAChR plasticity and behavioral responses to nicotine in rats
    Article Snippet: .. Total RNA was extracted and purified using RNeasy MiniKit (Qiagen, Valencia, CA). cDNA reverse transcription was performed using Quantitect Reverse Transcription Kit (Qiagen, Valencia, CA). mRNA expression levels were determined by qPCR using a QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA) according to manufacturer’s protocol on the iCycler IQ5 multicolor real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA). ..

    SYBR Green Assay:

    Article Title: The influence of neuropathic pain on nAChR plasticity and behavioral responses to nicotine in rats
    Article Snippet: .. Total RNA was extracted and purified using RNeasy MiniKit (Qiagen, Valencia, CA). cDNA reverse transcription was performed using Quantitect Reverse Transcription Kit (Qiagen, Valencia, CA). mRNA expression levels were determined by qPCR using a QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA) according to manufacturer’s protocol on the iCycler IQ5 multicolor real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA). ..

    Article Title: Nucleolar Organization, Ribosomal DNA Array Stability, and Acrocentric Chromosome Integrity Are Linked to Telomere Function
    Article Snippet: .. One confluent T25 flask per sample was used and the manufacturer's protocol was followed. qRT-PCRs were performed using Qiagen's QuantiFast SYBR Green PCR kit (Cat 204052) for GAPDH (primers: 5′-CTCATGACCACAGTCCATGCC-3′ , 5′-GCCATCCACAGTCTTCTGGGT-3′ ) , 45S pre-ribosomal RNA (primers: 5′-CTCCGTTATGGTAGCGCTGC-3′ , 5′-GCGGAACCCTCGCTTCTC-3′ ), and 28S ribosomal RNA (primers: 5′-CGACGACCCATTCGAACGTCT-3′ , 5′-CTCTCCGGAATCGAACCCTGA-3′ ) . .. Each primer pair was optimized (e.g. performing standard dilute and melt curve analyses) using HT1080 cDNA prior to performing qRT-PCR on the samples.

    Article Title: CVID Enteropathy is Characterized by Exceeding Low Mucosal IgA Levels and Interferon-Driven Inflammation Possibly Related to the Presence of a Pathobiont
    Article Snippet: .. Quantitative Real-Time PCR was done for the samples using QuantiFast SYBR Green PCR Kit (Qiagen, cat# 204056). qRT PCR set up used: samples were heated to 95°C, followed by 40 cycles of 95°C for 10 sec and 60°C for 30 sec. House-keeping gene used for normalization (SRSF11) was selected according to the procedure described in previous publication [ ]. .. Before been collected, cells in each well were washed with PBS and then lysed with Lysis Buffer from Amplex® Red Cholesterol Assay Kit (Invitrogen, cat#A12216), sonicated on ice 2 times 10 s each.

    Generated:

    Article Title: The influence of neuropathic pain on nAChR plasticity and behavioral responses to nicotine in rats
    Article Snippet: Total RNA was extracted and purified using RNeasy MiniKit (Qiagen, Valencia, CA). cDNA reverse transcription was performed using Quantitect Reverse Transcription Kit (Qiagen, Valencia, CA). mRNA expression levels were determined by qPCR using a QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA) according to manufacturer’s protocol on the iCycler IQ5 multicolor real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA). .. Amplification efficiency of each primer pair was determined based on the slope of standard curves generated by 1:10 serial dilutions of a cDNA sample pooled from each region examined ( ).

    Expressing:

    Article Title: The influence of neuropathic pain on nAChR plasticity and behavioral responses to nicotine in rats
    Article Snippet: .. Total RNA was extracted and purified using RNeasy MiniKit (Qiagen, Valencia, CA). cDNA reverse transcription was performed using Quantitect Reverse Transcription Kit (Qiagen, Valencia, CA). mRNA expression levels were determined by qPCR using a QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA) according to manufacturer’s protocol on the iCycler IQ5 multicolor real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA). ..

    Article Title: Nucleolar Organization, Ribosomal DNA Array Stability, and Acrocentric Chromosome Integrity Are Linked to Telomere Function
    Article Snippet: One confluent T25 flask per sample was used and the manufacturer's protocol was followed. qRT-PCRs were performed using Qiagen's QuantiFast SYBR Green PCR kit (Cat 204052) for GAPDH (primers: 5′-CTCATGACCACAGTCCATGCC-3′ , 5′-GCCATCCACAGTCTTCTGGGT-3′ ) , 45S pre-ribosomal RNA (primers: 5′-CTCCGTTATGGTAGCGCTGC-3′ , 5′-GCGGAACCCTCGCTTCTC-3′ ), and 28S ribosomal RNA (primers: 5′-CGACGACCCATTCGAACGTCT-3′ , 5′-CTCTCCGGAATCGAACCCTGA-3′ ) . .. Fold change was calculated using the following formulas: ΔCt = target Ct - endogenous Ct (i.e. GAPDH) ΔΔCt = ΔCt sample - ΔCt Calibrator (i.e. T19 control) Fold Change (Relative Expression) = 2ΔΔCt

    Article Title: CVID Enteropathy is Characterized by Exceeding Low Mucosal IgA Levels and Interferon-Driven Inflammation Possibly Related to the Presence of a Pathobiont
    Article Snippet: For comparison to E-CVID, expression of genes from E-CVID signature was compared between unstimulated cells and cells stimulated with both interferons as specified above. .. Quantitative Real-Time PCR was done for the samples using QuantiFast SYBR Green PCR Kit (Qiagen, cat# 204056). qRT PCR set up used: samples were heated to 95°C, followed by 40 cycles of 95°C for 10 sec and 60°C for 30 sec. House-keeping gene used for normalization (SRSF11) was selected according to the procedure described in previous publication [ ].

    Polymerase Chain Reaction:

    Article Title: The influence of neuropathic pain on nAChR plasticity and behavioral responses to nicotine in rats
    Article Snippet: .. Total RNA was extracted and purified using RNeasy MiniKit (Qiagen, Valencia, CA). cDNA reverse transcription was performed using Quantitect Reverse Transcription Kit (Qiagen, Valencia, CA). mRNA expression levels were determined by qPCR using a QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA) according to manufacturer’s protocol on the iCycler IQ5 multicolor real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA). ..

    Article Title: Nucleolar Organization, Ribosomal DNA Array Stability, and Acrocentric Chromosome Integrity Are Linked to Telomere Function
    Article Snippet: .. One confluent T25 flask per sample was used and the manufacturer's protocol was followed. qRT-PCRs were performed using Qiagen's QuantiFast SYBR Green PCR kit (Cat 204052) for GAPDH (primers: 5′-CTCATGACCACAGTCCATGCC-3′ , 5′-GCCATCCACAGTCTTCTGGGT-3′ ) , 45S pre-ribosomal RNA (primers: 5′-CTCCGTTATGGTAGCGCTGC-3′ , 5′-GCGGAACCCTCGCTTCTC-3′ ), and 28S ribosomal RNA (primers: 5′-CGACGACCCATTCGAACGTCT-3′ , 5′-CTCTCCGGAATCGAACCCTGA-3′ ) . .. Each primer pair was optimized (e.g. performing standard dilute and melt curve analyses) using HT1080 cDNA prior to performing qRT-PCR on the samples.

    Article Title: CVID Enteropathy is Characterized by Exceeding Low Mucosal IgA Levels and Interferon-Driven Inflammation Possibly Related to the Presence of a Pathobiont
    Article Snippet: .. Quantitative Real-Time PCR was done for the samples using QuantiFast SYBR Green PCR Kit (Qiagen, cat# 204056). qRT PCR set up used: samples were heated to 95°C, followed by 40 cycles of 95°C for 10 sec and 60°C for 30 sec. House-keeping gene used for normalization (SRSF11) was selected according to the procedure described in previous publication [ ]. .. Before been collected, cells in each well were washed with PBS and then lysed with Lysis Buffer from Amplex® Red Cholesterol Assay Kit (Invitrogen, cat#A12216), sonicated on ice 2 times 10 s each.

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  • About
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  • Team
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  • Contact
  • Bioz Stars
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  • 90
    Qiagen quantifast sybr green pcr kit
    Quantifast Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantifast sybr green pcr kit/product/Qiagen
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