quant it rna kit  (Thermo Fisher)


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    Name:
    Quant iT RNA Assay Kit
    Description:
    The new Quant iT RNA Assay Kit Broad Range provides an accurate and selective method for quantitation of high abundance RNA samples Whereas the original Quant iT RNA Kit has great sensitivity the Quant iT RNA Broad Range Kit is ideal for microarray samples where the concentration of RNA is higher With the Quant iT RNA Broad Range Kit dilution of the RNA samples prior to quantitation is not required The kit is specific for RNA and will not quantitate DNA protein or free nucleotides
    Catalog Number:
    q10213
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|RNA Quantitation|Nucleic Acid Quantitation
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher quant it rna kit
    The new Quant iT RNA Assay Kit Broad Range provides an accurate and selective method for quantitation of high abundance RNA samples Whereas the original Quant iT RNA Kit has great sensitivity the Quant iT RNA Broad Range Kit is ideal for microarray samples where the concentration of RNA is higher With the Quant iT RNA Broad Range Kit dilution of the RNA samples prior to quantitation is not required The kit is specific for RNA and will not quantitate DNA protein or free nucleotides
    https://www.bioz.com/result/quant it rna kit/product/Thermo Fisher
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    quant it rna kit - by Bioz Stars, 2020-08
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Satellite DNA Modulates Gene Expression in the Beetle Tribolium castaneum after Heat Stress
    Article Snippet: .. RNA was additionally digested with Turbo DNase (Ambion), quantified with the Quant-IT RNA assay kit using Qubit fluorometer (Invitrogen), quality-checked on gel, and PCR-checked for the presence of TCAST DNA. .. RNA (approximately 1 μg) was reverse transcribed using PrimeScript RT-PCR Kit (Takara) in 10 μl reaction using random primers.

    Concentration Assay:

    Article Title: Deficit of Kcnma1 mRNA expression in the dentate gyrus of epileptic rats
    Article Snippet: .. The concentration and purity of total RNA for each sample was determined by the Quant-iT RNA Assay Kit and Q32857 Qubit fluorometer (Carlsbad, Invitrogen, California, USA) and confirmed by optical density measurements at 260 and 280nm using a BioMate 5 UV-visible spectrophotometer (Thermo Spectronic, Waltham, Massachusetts, USA). .. The integrity of the extracted RNA was confirmed by electrophoresis under denaturating condition [ ].

    Article Title: Requirements for resuming translation in chimeric transfer-messenger RNAs of Escherichia coli and Mycobacterium tuberculosis
    Article Snippet: .. Its concentration was measured using Quant-iT RNA assay kit from Invitrogen. ..

    Article Title: Semen miRNAs Contained in Exosomes as Non-Invasive Biomarkers for Prostate Cancer Diagnosis
    Article Snippet: .. RNA concentration was calculated by using the QUBIT fluorometer and the Quant-iT RNA Assay kit (Invitrogen; California, USA). .. All RNA samples presented an OD 260/280 nm ratio ≥ 1,7 when using a Nanodrop UV-Vis spectrophotometer (Thermo Fisher Scientific; Massachusetts, USA).

    Purification:

    Article Title: Messenger RNA exchange between scions and rootstocks in grafted grapevines
    Article Snippet: .. Purified mRNAs were quantified using Quant-iT RNA assay kit with the Qubit fluorometer (Life Technologies). ..

    Spectrophotometry:

    Article Title: Deficit of Kcnma1 mRNA expression in the dentate gyrus of epileptic rats
    Article Snippet: .. The concentration and purity of total RNA for each sample was determined by the Quant-iT RNA Assay Kit and Q32857 Qubit fluorometer (Carlsbad, Invitrogen, California, USA) and confirmed by optical density measurements at 260 and 280nm using a BioMate 5 UV-visible spectrophotometer (Thermo Spectronic, Waltham, Massachusetts, USA). .. The integrity of the extracted RNA was confirmed by electrophoresis under denaturating condition [ ].

    Staining:

    Article Title: Splenic RNA and microRNA mimics promote complement factor B production and the alternative pathway activation via innate immune signaling
    Article Snippet: .. Bovine pancreas RNase A, lipofectamine 3000, TRIzol LS, SYTO RNASelect Green fluorescent cell stain, and Quant-iT™ RNA assay kit were from Invitrogen Life Technology (Carlsbad, CA). .. The TLR ligands, poly(I:C), Pam3Cys and CpG, were from Enzo Life (Plymouth Meeting, PA).

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  • 99
    Thermo Fisher quant it ribogreen assay kit
    Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) <t>Ribogreen</t> assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p
    Quant It Ribogreen Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it ribogreen assay kit/product/Thermo Fisher
    Average 99 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    quant it ribogreen assay kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher quant it picogreen assay kit
    DNA quantity per scaffold at 3 and 7 days, assessed by <t>PicoGreen</t> assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points
    Quant It Picogreen Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it picogreen assay kit/product/Thermo Fisher
    Average 99 stars, based on 2211 article reviews
    Price from $9.99 to $1999.99
    quant it picogreen assay kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) Ribogreen assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p

    Journal: ACS nano

    Article Title: Zwitterionic Nanocarrier Surface Chemistry Improves siRNA Tumor Delivery and Silencing Activity Relative to Polyethylene Glycol

    doi: 10.1021/acsnano.7b01110

    Figure Lengend Snippet: Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) Ribogreen assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p

    Article Snippet: Polyplex encapsulation efficiency at various N+ :P− ratios was evaluated using a Quant-iT Ribogreen assay kit (ThermoFisher, USA).

    Techniques: Incubation

    Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Journal: PLoS ONE

    Article Title: Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes

    doi: 10.1371/journal.pone.0195969

    Figure Lengend Snippet: Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Article Snippet: RNA was quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific).

    Techniques: Transfection, Expressing, Negative Control, Western Blot, Plasmid Preparation, Isolation, Purification, SDS Page, Staining, Pull Down Assay, Incubation

    DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points

    Journal: Tissue Engineering and Regenerative Medicine

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering

    doi: 10.1007/s13770-017-0107-5

    Figure Lengend Snippet: DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points

    Article Snippet: Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions.

    Techniques: Picogreen Assay

    DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ PicoGreen ® dsDNA assay. The results are presented as means ± SD. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality

    doi: 10.3390/ijms19010227

    Figure Lengend Snippet: DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ PicoGreen ® dsDNA assay. The results are presented as means ± SD. *** p

    Article Snippet: For quantification, DNA was extracted using the PureLink™ genomic DNA mini kit (Thermo Fisher, Ghent, Belgium, K1820-01) and quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher, P7589) following the manufacturer’s instructions.

    Techniques: Staining, Picogreen Assay