quant it rna assay kit  (Thermo Fisher)


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    Thermo Fisher quant it rna assay kit
    Quant It Rna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it rna assay kit/product/Thermo Fisher
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    quant it rna assay kit - by Bioz Stars, 2020-04
    96/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: During the process of RNA purification, genomic DNA was removed effectively in a single centrifugation step. .. RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm.

    Amplification:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm. .. The RT reaction mixture was then diluted 1:10 with nuclease-free water and used for PCR amplification of MMP cDNA in the presence of the primers.

    Synthesized:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm. .. First-strand cDNA (cDNA) was synthesized with the iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) using 20 μl reaction mixture containing 0.25 μg total RNA, 4 μl 5× iScript reaction mixture, and 1 μl iScript RT.

    Article Title: Identification of the Efflux Transporter of the Fluoroquinolone Antibiotic Ciprofloxacin in Murine Macrophages: Studies with Ciprofloxacin-Resistant Cells ▿
    Article Snippet: .. Total RNA was purified with Turbo DNase (Ambion, Austin, TX), and the concentration was measured with a Qubit fluorimeter, using a Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized using 1 μg of total purified RNA and random hexamer primers (Promega reverse transcription system; Promega Co., Madison, WI). .. Primer pairs for all Mrp (also referred to as Abcc ) genes investigated were designed using Primer 3 and Autoprime programs , taking into account exon-intron boundaries to prevent genomic DNA amplification.

    Construct:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The libraries were constructed on the Sciclone NGS Workstation (PerkinElmer) following the Illumina TruSeq RNA sample preparation v2 guide and using the TruSeq RNA Library Preparation Kit v2 (Illumina). .. The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size.

    Electrophoresis:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: .. The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Microarray:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: Paragraph title: Microarray analysis ... RNA concentration was determined using the Quant-iT RNA assay kit (Invitrogen).

    Random Hexamer Labeling:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Article Title: Identification of the Efflux Transporter of the Fluoroquinolone Antibiotic Ciprofloxacin in Murine Macrophages: Studies with Ciprofloxacin-Resistant Cells ▿
    Article Snippet: .. Total RNA was purified with Turbo DNase (Ambion, Austin, TX), and the concentration was measured with a Qubit fluorimeter, using a Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized using 1 μg of total purified RNA and random hexamer primers (Promega reverse transcription system; Promega Co., Madison, WI). .. Primer pairs for all Mrp (also referred to as Abcc ) genes investigated were designed using Primer 3 and Autoprime programs , taking into account exon-intron boundaries to prevent genomic DNA amplification.

    Expressing:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: RNA concentration was determined using the Quant-iT RNA assay kit (Invitrogen). .. RNA labeling, subsequent microarray hybridization, fluidics, and scanning for all samples were performed by Expression Analysis, Inc. (Durham, NC).

    Hybridization:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: RNA concentration was determined using the Quant-iT RNA assay kit (Invitrogen). .. RNA labeling, subsequent microarray hybridization, fluidics, and scanning for all samples were performed by Expression Analysis, Inc. (Durham, NC).

    Flow Cytometry:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Ligation:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: Briefly, all samples were prepared according to the following steps: (1) manual muscle fiber isolation, (2) muscle fiber clipping to allow for fiber-type identification via SDS-PAGE , (3) muscle fiber pooling of alike fiber-types, (4) RNA extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) and RNA quantification (Quant-iT RNA assay kit, Invitrogen, Carlsbad, CA). .. Using the ThruPLEX® DNA-seq kit (Takara), 1–2 ng of double-stranded cDNA was end-repaired and A-tailed to prepare for adaptor ligation.

    Generated:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: RNA-sequencing of slow- and fast-twitch muscle fiber samples Eighteen fiber-type specific samples were generated as previously described . .. Briefly, all samples were prepared according to the following steps: (1) manual muscle fiber isolation, (2) muscle fiber clipping to allow for fiber-type identification via SDS-PAGE , (3) muscle fiber pooling of alike fiber-types, (4) RNA extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) and RNA quantification (Quant-iT RNA assay kit, Invitrogen, Carlsbad, CA).

    other:

    Article Title: Gene expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ
    Article Snippet: HBRR and UHRR stocks were measured with a Quant-iT RNA Assay Kit (Thermo Fisher Scientific ) on a Qubit fluorometer, and these concentrations were used to calculate dilutions.

    DNA Sequencing:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: Briefly, all samples were prepared according to the following steps: (1) manual muscle fiber isolation, (2) muscle fiber clipping to allow for fiber-type identification via SDS-PAGE , (3) muscle fiber pooling of alike fiber-types, (4) RNA extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) and RNA quantification (Quant-iT RNA assay kit, Invitrogen, Carlsbad, CA). .. Using the ThruPLEX® DNA-seq kit (Takara), 1–2 ng of double-stranded cDNA was end-repaired and A-tailed to prepare for adaptor ligation.

    Sequencing:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: Synthesis of cDNA and RNA sequencing cDNA library preparation and sequencing were carried out by the Earlham Institute, formerly The Genome Analysis Centre (TGAC) Norwich, UK. .. The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size.

    RNA Sequencing Assay:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: Paragraph title: Synthesis of cDNA and RNA sequencing ... The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size.

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: Paragraph title: RNA-sequencing of slow- and fast-twitch muscle fiber samples ... Briefly, all samples were prepared according to the following steps: (1) manual muscle fiber isolation, (2) muscle fiber clipping to allow for fiber-type identification via SDS-PAGE , (3) muscle fiber pooling of alike fiber-types, (4) RNA extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) and RNA quantification (Quant-iT RNA assay kit, Invitrogen, Carlsbad, CA).

    Magnetic Beads:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Isolation:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: Total RNA was isolated from cells using the RNeasy Plus Mini kit (Qiagen, Santa Clarita, CA, USA). .. RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm.

    Article Title: Identification of the Efflux Transporter of the Fluoroquinolone Antibiotic Ciprofloxacin in Murine Macrophages: Studies with Ciprofloxacin-Resistant Cells ▿
    Article Snippet: Paragraph title: Isolation of RNA and synthesis of cDNA. ... Total RNA was purified with Turbo DNase (Ambion, Austin, TX), and the concentration was measured with a Qubit fluorimeter, using a Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized using 1 μg of total purified RNA and random hexamer primers (Promega reverse transcription system; Promega Co., Madison, WI).

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: .. Briefly, all samples were prepared according to the following steps: (1) manual muscle fiber isolation, (2) muscle fiber clipping to allow for fiber-type identification via SDS-PAGE , (3) muscle fiber pooling of alike fiber-types, (4) RNA extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) and RNA quantification (Quant-iT RNA assay kit, Invitrogen, Carlsbad, CA). .. Cofactor Genomics (St Louis, MO) performed the library construction and RNA sequencing as previously described .

    Labeling:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: RNA concentration was determined using the Quant-iT RNA assay kit (Invitrogen). .. RNA labeling, subsequent microarray hybridization, fluidics, and scanning for all samples were performed by Expression Analysis, Inc. (Durham, NC).

    Purification:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: During the process of RNA purification, genomic DNA was removed effectively in a single centrifugation step. .. RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm.

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Article Title: Identification of the Efflux Transporter of the Fluoroquinolone Antibiotic Ciprofloxacin in Murine Macrophages: Studies with Ciprofloxacin-Resistant Cells ▿
    Article Snippet: .. Total RNA was purified with Turbo DNase (Ambion, Austin, TX), and the concentration was measured with a Qubit fluorimeter, using a Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized using 1 μg of total purified RNA and random hexamer primers (Promega reverse transcription system; Promega Co., Madison, WI). .. Primer pairs for all Mrp (also referred to as Abcc ) genes investigated were designed using Primer 3 and Autoprime programs , taking into account exon-intron boundaries to prevent genomic DNA amplification.

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: Each total RNA sample was purified using the RNeasy Micro Kit (Qiagen, Valencia, CA). .. RNA concentration was determined using the Quant-iT RNA assay kit (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm. .. The RT reaction mixture was then diluted 1:10 with nuclease-free water and used for PCR amplification of MMP cDNA in the presence of the primers.

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    cDNA Library Assay:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: .. The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    SDS Page:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: .. Briefly, all samples were prepared according to the following steps: (1) manual muscle fiber isolation, (2) muscle fiber clipping to allow for fiber-type identification via SDS-PAGE , (3) muscle fiber pooling of alike fiber-types, (4) RNA extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) and RNA quantification (Quant-iT RNA assay kit, Invitrogen, Carlsbad, CA). .. Cofactor Genomics (St Louis, MO) performed the library construction and RNA sequencing as previously described .

    Software:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Real-time Polymerase Chain Reaction:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: Paragraph title: Real-time PCR ... RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm.

    RNA Extraction:

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: .. Briefly, all samples were prepared according to the following steps: (1) manual muscle fiber isolation, (2) muscle fiber clipping to allow for fiber-type identification via SDS-PAGE , (3) muscle fiber pooling of alike fiber-types, (4) RNA extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) and RNA quantification (Quant-iT RNA assay kit, Invitrogen, Carlsbad, CA). .. Cofactor Genomics (St Louis, MO) performed the library construction and RNA sequencing as previously described .

    Selection:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Sample Prep:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The libraries were constructed on the Sciclone NGS Workstation (PerkinElmer) following the Illumina TruSeq RNA sample preparation v2 guide and using the TruSeq RNA Library Preparation Kit v2 (Illumina). .. The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size.

    Next-Generation Sequencing:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The libraries were constructed on the Sciclone NGS Workstation (PerkinElmer) following the Illumina TruSeq RNA sample preparation v2 guide and using the TruSeq RNA Library Preparation Kit v2 (Illumina). .. The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size.

    dsDNA Assay:

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: .. The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Concentration Assay:

    Article Title: Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation
    Article Snippet: .. RNA concentration was determined using a Quant-iT™ RNA assay kit from Invitrogen Corp. RNA purity was determined by the ratio of absorbance readings of samples at 260 and 280 nm. .. First-strand cDNA (cDNA) was synthesized with the iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) using 20 μl reaction mixture containing 0.25 μg total RNA, 4 μl 5× iScript reaction mixture, and 1 μl iScript RT.

    Article Title: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
    Article Snippet: The library preparation involved several quality control analysis steps, including the use of the Quant-iT™ RNA Assay Kit (Life Technologies) for RNA quantification, the Quant-iT™ dsDNA Assay Kit (Life Technologies) for double-stranded DNA quantification as well as the LabChip GX Automated Electrophoresis System (PerkinElmer) and High Sensitivity DNA kit (Agilent) for RNA/dsDNA quantification and verification of the cDNA library insert size. .. Shortly, the RNA-seq workflow included (1) purification and fragmentation of mRNA from 1 μg of total RNA with a poly(A)-pull down using oligo-dT attached magnetic beads; (2 ) first strand cDNA synthesis with random hexamer primers and SuperScript II reverse transcriptase (Invitrogen); (3) second strand cDNA synthesis using DNA polymerase I and RNase H; (4) cDNA end repair/blunting; (5) cDNA fragment 3′ end adenylation; (6) ligation of multiple indexing adapters to cDNA fragments and purification of ligated products via bead-based size selection using AMPure XP beads (Beckman Coulter); (7) PCR enrichment of adapter-ligated cDNA fragments with a PCR primer cocktail that anneals to the adapter ends; (8) quantitative and qualitative validation of cDNA library; (9) normalisation and equimolar pooling of indexed DNA libraries; (10) dilution of library pool to a final concentration of 10 pM and spiking of each library pool with 1% PhiX Control v3 (Illumina); (11) flow cell clustering using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina); and (12) sequencing of flow cell using the Illumina HiSeq™ 2000 platform with the TruSeq SBS Kit v3-HS (Illumina) and HiSeq Control Software 2.2.58 and RTA 1.18.64.

    Article Title: Identification of the Efflux Transporter of the Fluoroquinolone Antibiotic Ciprofloxacin in Murine Macrophages: Studies with Ciprofloxacin-Resistant Cells ▿
    Article Snippet: .. Total RNA was purified with Turbo DNase (Ambion, Austin, TX), and the concentration was measured with a Qubit fluorimeter, using a Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized using 1 μg of total purified RNA and random hexamer primers (Promega reverse transcription system; Promega Co., Madison, WI). .. Primer pairs for all Mrp (also referred to as Abcc ) genes investigated were designed using Primer 3 and Autoprime programs , taking into account exon-intron boundaries to prevent genomic DNA amplification.

    Article Title: Single-cell transcriptional profiles in human skeletal muscle
    Article Snippet: .. RNA concentration was determined using the Quant-iT RNA assay kit (Invitrogen). .. RNA labeling, subsequent microarray hybridization, fluidics, and scanning for all samples were performed by Expression Analysis, Inc. (Durham, NC).

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  • 99
    Thermo Fisher quant it picogreen dsdna assay kit
    DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ <t>PicoGreen</t> ® <t>dsDNA</t> assay. The results are presented as means ± SD. *** p
    Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it picogreen dsdna assay kit/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    quant it picogreen dsdna assay kit - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Thermo Fisher quant it ribogreen assay kit
    Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) <t>Ribogreen</t> assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p
    Quant It Ribogreen Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it ribogreen assay kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quant it ribogreen assay kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ PicoGreen ® dsDNA assay. The results are presented as means ± SD. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality

    doi: 10.3390/ijms19010227

    Figure Lengend Snippet: DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ PicoGreen ® dsDNA assay. The results are presented as means ± SD. *** p

    Article Snippet: For quantification, DNA was extracted using the PureLink™ genomic DNA mini kit (Thermo Fisher, Ghent, Belgium, K1820-01) and quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher, P7589) following the manufacturer’s instructions.

    Techniques: Staining, Picogreen Assay

    DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points

    Journal: Tissue Engineering and Regenerative Medicine

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering

    doi: 10.1007/s13770-017-0107-5

    Figure Lengend Snippet: DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points

    Article Snippet: Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions.

    Techniques: Picogreen Assay

    Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A to C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD; n = 3 isolations per sample.

    Journal: Science Advances

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    doi: 10.1126/sciadv.1600502

    Figure Lengend Snippet: Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A to C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD; n = 3 isolations per sample.

    Article Snippet: The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific.

    Techniques: Nucleic Acid Concentration, Concentration Assay, Isolation

    Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) Ribogreen assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p

    Journal: ACS nano

    Article Title: Zwitterionic Nanocarrier Surface Chemistry Improves siRNA Tumor Delivery and Silencing Activity Relative to Polyethylene Glycol

    doi: 10.1021/acsnano.7b01110

    Figure Lengend Snippet: Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) Ribogreen assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p

    Article Snippet: Polyplex encapsulation efficiency at various N+ :P− ratios was evaluated using a Quant-iT Ribogreen assay kit (ThermoFisher, USA).

    Techniques: Incubation