quant it rna assay kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Quant-iT RNA Assay Kit
    Description:
    The new Quant-iT RNA Assay Kit, Broad Range, provides an accurate and selective method for quantitation of high-abundance RNA samples. Whereas the original Quant-iT RNA Kit has great sensitivity, the Quant-iT RNA Broad Range Kit is ideal for microarray samples where the concentration of RNA is higher. With the Quant-iT RNA Broad Range Kit, dilution of the RNA samples prior to quantitation is not required. The kit is specific for RNA and will not quantitate DNA, protein or free nucleotides.
    Catalog Number:
    Q10213
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|RNA Quantitation|Nucleic Acid Quantitation
    Size:
    1 000 assays
    Category:
    Kits and Assays, DNA⁄RNA Quantitation Assay Kits
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher quant it rna assay kit
    In unimmunized animals HEL-specific T cells are present in the naive, regulatory, effector memory, and central memory T cell compartments. (A) Splenocytes from antigen-naive, 12-to-14-week-old BALB/c mice were FACS sorted to isolate naive, regulatory, effector memory, and central memory T cells using antibodies specific to CD4, CD25, CD127, CD44, and CD62L. <t>RNA</t> was then harvested from the isolated T cells and <t>cDNA</t> was synthesized using a TCR-specific primer to the constant region of the TCR β gene. To minimize amplification bias, the cDNA was then split into multiple PCR reaction tubes. Primers to Vβ8.2 and Jβ1.5 and high high-fidelity Taq DNA polymerase were then used to amplify the CDR3 region of just the Vβ8.2Jβ1.5 subpopulation of T cells. To minimize amplification bias, the number of PCR cyles was optimized by real-time PCR to ensure that PCR reactions were stopped during the linear phase of the amplification. Amplified products were used to generate sequencing libraries. 9,850,078 total reads were obtained from the central memory T cell library. These sequences were then filtered to remove sequences with incomplete CDR3 regions, N’s, and frameshifts. Sequences were also removed if they did not meet a Phred quality score cut-off of 30, or if their forward and reverse sequences did not match perfectly. The remaining 4,622,141 (central memory) CDR3 sequences were then analyzed to determine the frequency of the HEL-specific sequence. The same process was repeated for the other T cell subpopulations. Results are representative of three independent experiments. (B) The germline Vβ8.2 sequence, upstream of the CDR3 region, was used to determine the frequency of sequencing/amplification errors. Similarity scores for the different reads, and the read’s copy number are represented graphically against sequence rank order; the reads were ranked based upon their copy number with “1” being the most abundant read. Results are representative of three independent experiments. (C) Graphs of copy number vs. distinct CDR3 sequence revealed that the HEL- specific Vβ8.2Jβ1.5 CDR3 sequence was present within the naive, regulatory, effector memory, and central memory T cell populations and that the sequence was not expanded when compared with other CDR3 sequences. D96 cut off values(red dot lines) were calculated to identify CDR3 sequences that resided at unacceptably low frequencies, i.e. those that had an increased possibility of originating from amplification or sequencing errors. Results are representative of three independent experiments. (D) In silico spectratyping of CDR3 lengths revealed Gaussian distributions for the naive, regulatory, central memory, and effector memory Vβ8.2Jβ1.5 spectra. Results are representative of at lest three independent experiments.
    The new Quant-iT RNA Assay Kit, Broad Range, provides an accurate and selective method for quantitation of high-abundance RNA samples. Whereas the original Quant-iT RNA Kit has great sensitivity, the Quant-iT RNA Broad Range Kit is ideal for microarray samples where the concentration of RNA is higher. With the Quant-iT RNA Broad Range Kit, dilution of the RNA samples prior to quantitation is not required. The kit is specific for RNA and will not quantitate DNA, protein or free nucleotides.
    https://www.bioz.com/result/quant it rna assay kit/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    quant it rna assay kit - by Bioz Stars, 2019-10
    99/100 stars

    Images

    1) Product Images from "CD4+ Virtual Memory: Antigen-inexperienced T cells reside in the naïve, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity"

    Article Title: CD4+ Virtual Memory: Antigen-inexperienced T cells reside in the naïve, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity

    Journal:

    doi: 10.1016/j.jaut.2016.11.001

    In unimmunized animals HEL-specific T cells are present in the naive, regulatory, effector memory, and central memory T cell compartments. (A) Splenocytes from antigen-naive, 12-to-14-week-old BALB/c mice were FACS sorted to isolate naive, regulatory, effector memory, and central memory T cells using antibodies specific to CD4, CD25, CD127, CD44, and CD62L. RNA was then harvested from the isolated T cells and cDNA was synthesized using a TCR-specific primer to the constant region of the TCR β gene. To minimize amplification bias, the cDNA was then split into multiple PCR reaction tubes. Primers to Vβ8.2 and Jβ1.5 and high high-fidelity Taq DNA polymerase were then used to amplify the CDR3 region of just the Vβ8.2Jβ1.5 subpopulation of T cells. To minimize amplification bias, the number of PCR cyles was optimized by real-time PCR to ensure that PCR reactions were stopped during the linear phase of the amplification. Amplified products were used to generate sequencing libraries. 9,850,078 total reads were obtained from the central memory T cell library. These sequences were then filtered to remove sequences with incomplete CDR3 regions, N’s, and frameshifts. Sequences were also removed if they did not meet a Phred quality score cut-off of 30, or if their forward and reverse sequences did not match perfectly. The remaining 4,622,141 (central memory) CDR3 sequences were then analyzed to determine the frequency of the HEL-specific sequence. The same process was repeated for the other T cell subpopulations. Results are representative of three independent experiments. (B) The germline Vβ8.2 sequence, upstream of the CDR3 region, was used to determine the frequency of sequencing/amplification errors. Similarity scores for the different reads, and the read’s copy number are represented graphically against sequence rank order; the reads were ranked based upon their copy number with “1” being the most abundant read. Results are representative of three independent experiments. (C) Graphs of copy number vs. distinct CDR3 sequence revealed that the HEL- specific Vβ8.2Jβ1.5 CDR3 sequence was present within the naive, regulatory, effector memory, and central memory T cell populations and that the sequence was not expanded when compared with other CDR3 sequences. D96 cut off values(red dot lines) were calculated to identify CDR3 sequences that resided at unacceptably low frequencies, i.e. those that had an increased possibility of originating from amplification or sequencing errors. Results are representative of three independent experiments. (D) In silico spectratyping of CDR3 lengths revealed Gaussian distributions for the naive, regulatory, central memory, and effector memory Vβ8.2Jβ1.5 spectra. Results are representative of at lest three independent experiments.
    Figure Legend Snippet: In unimmunized animals HEL-specific T cells are present in the naive, regulatory, effector memory, and central memory T cell compartments. (A) Splenocytes from antigen-naive, 12-to-14-week-old BALB/c mice were FACS sorted to isolate naive, regulatory, effector memory, and central memory T cells using antibodies specific to CD4, CD25, CD127, CD44, and CD62L. RNA was then harvested from the isolated T cells and cDNA was synthesized using a TCR-specific primer to the constant region of the TCR β gene. To minimize amplification bias, the cDNA was then split into multiple PCR reaction tubes. Primers to Vβ8.2 and Jβ1.5 and high high-fidelity Taq DNA polymerase were then used to amplify the CDR3 region of just the Vβ8.2Jβ1.5 subpopulation of T cells. To minimize amplification bias, the number of PCR cyles was optimized by real-time PCR to ensure that PCR reactions were stopped during the linear phase of the amplification. Amplified products were used to generate sequencing libraries. 9,850,078 total reads were obtained from the central memory T cell library. These sequences were then filtered to remove sequences with incomplete CDR3 regions, N’s, and frameshifts. Sequences were also removed if they did not meet a Phred quality score cut-off of 30, or if their forward and reverse sequences did not match perfectly. The remaining 4,622,141 (central memory) CDR3 sequences were then analyzed to determine the frequency of the HEL-specific sequence. The same process was repeated for the other T cell subpopulations. Results are representative of three independent experiments. (B) The germline Vβ8.2 sequence, upstream of the CDR3 region, was used to determine the frequency of sequencing/amplification errors. Similarity scores for the different reads, and the read’s copy number are represented graphically against sequence rank order; the reads were ranked based upon their copy number with “1” being the most abundant read. Results are representative of three independent experiments. (C) Graphs of copy number vs. distinct CDR3 sequence revealed that the HEL- specific Vβ8.2Jβ1.5 CDR3 sequence was present within the naive, regulatory, effector memory, and central memory T cell populations and that the sequence was not expanded when compared with other CDR3 sequences. D96 cut off values(red dot lines) were calculated to identify CDR3 sequences that resided at unacceptably low frequencies, i.e. those that had an increased possibility of originating from amplification or sequencing errors. Results are representative of three independent experiments. (D) In silico spectratyping of CDR3 lengths revealed Gaussian distributions for the naive, regulatory, central memory, and effector memory Vβ8.2Jβ1.5 spectra. Results are representative of at lest three independent experiments.

    Techniques Used: Mouse Assay, FACS, Isolation, Synthesized, Amplification, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Sequencing, In Silico

    Related Articles

    Clone Assay:

    Article Title: A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA
    Article Snippet: To determine the copy number of viral genomes a synthetic calibrator was developed, which comprises a T7 RNA polymerase promoter and the target sequences for the RT-qPCRs of EEEV, WEEV, and VEEV ( ) cloned into the pCR2.1 vector (Eurofins MWG Operon, Germany). .. The RNA concentration was estimated with the Quant-It™ RNA Assay Kit, Broad-Range (Invitrogen).

    Centrifugation:

    Article Title: Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria
    Article Snippet: After centrifugation, pellets were stored at − 80 °C until RNA purification. .. The resulting RNA was quantified using a Qubit 2.0 fluorometer using Life Technologies Quant-iT™ RNA Assay Kit.

    Amplification:

    Article Title: A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA
    Article Snippet: The EEEV and WEEV sequences include targets for primer and probes adopted unmodified from the literature [ ] , but the corresponding probe target sequences were placed on the complementary strand in order to generate a unique (different) amplicon sequence, discriminable from the original virus sequence yet maintaining the same nucleotide composition. .. The RNA concentration was estimated with the Quant-It™ RNA Assay Kit, Broad-Range (Invitrogen).

    Article Title: Elevation of MiR-9–3p suppresses the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via down-regulating FN1, ITGB1 and ITGAV
    Article Snippet: The Quant-iT RNA Assay Kit (Molecular Probes, Eugene, OR, USA) was used to detect the RNA concentration. .. For gene expression, the RNA was hybridized to Human Exon 1.0 ST Arrays (Affymetrix, Santa Clara, CA, USA) and analyzed as described previously For RT-qPCR analysis, total RNAs extracted from frozen NPC tissues or cellsusing RNA extraction Kit (Invitrogen) were reverse-transcribed to cDNAs using RT Kit (Takara, Tokyo, Japan).

    Synthesized:

    Article Title: CD4+ Virtual Memory: Antigen-inexperienced T cells reside in the naïve, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity
    Article Snippet: Total RNA was isolated using the Qiagen RNEasy Plus Kit (Qiagen) and treated with RNA-free DNase I (Ambion) to remove genomic DNA. .. RNA concentrations were quantified using a Qubit® Fluorometer and the Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized by reverse transcription using the SuperScript® III First- Strand Synthesis System for RT-PCR (Invitrogen) according to the manufacturer’s instructions. .. TCR CDR3 spectrotyping analysis was performed as has been described in detail elsewhere [ ].

    Article Title: Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana
    Article Snippet: The synthesized products were suspended at a final concentration of 0.1 µg µL−1 in RNase-free water supplemented with Recombinant RNasin (ribonuclease inhibitor; Promega) and stored at −20°C until used ( ). .. The cRNA was quantified by fluorescence using a Quant-iT RNA assay kit (Invitrogen).

    Article Title: Absolute measurement of gene transcripts with Selfie-digital PCR
    Article Snippet: Polyadenylated Gsk3 β RNA of a fixed length was synthesized from linearized vector template by using T7 RNA polymerase (EO0111, Thermo-Fisher Scientific). .. The full-length transcript was gel-purified and quantified using a Quant-iT™ RNA Assay Kit (Q33140 Thermo-Fisher Scientific).

    Picogreen Assay:

    Article Title: Absolute measurement of gene transcripts with Selfie-digital PCR
    Article Snippet: To determine the concentration of the DNA template for accuracy studies, we used a Quant-iT PicoGreen dsDNA Assay Kit (P7589, Thermo-Fisher Scientific). .. The full-length transcript was gel-purified and quantified using a Quant-iT™ RNA Assay Kit (Q33140 Thermo-Fisher Scientific).

    Quantitative RT-PCR:

    Article Title: Circulating Plasma Extracellular Vesicles from Septic Mice Induce Inflammation via MicroRNA- and TLR7-Dependent Mechanisms
    Article Snippet: Paragraph title: EV miRNA detection by quantitative RT-PCR ... RNA pellets were resuspended in 14 μl of RNase-free water and quantified using Quant-iT RNA Assay Kit (Invitrogen, Waltham, MA) as reported previously ( , ).

    Article Title: Imatinib Affects the Expression of SLC22A1 in a Non-Linear Concentration-Dependent Manner Within 24 Hours
    Article Snippet: The concentration of extracted RNA was determined using the Quant-iT RNA Assay Kit according to manufacturer’s instructions (Invitrogen). .. The concentration of extracted RNA was determined using the Quant-iT RNA Assay Kit according to manufacturer’s instructions (Invitrogen).

    Article Title: Elevation of MiR-9–3p suppresses the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via down-regulating FN1, ITGB1 and ITGAV
    Article Snippet: Paragraph title: Microarray analysis and quantitative real time pcr (RT-qPCR) ... The Quant-iT RNA Assay Kit (Molecular Probes, Eugene, OR, USA) was used to detect the RNA concentration.

    SYBR Green Assay:

    Article Title: A Novel Mouse Model of Schistosoma haematobium Egg-Induced Immunopathology
    Article Snippet: The concentration of RNA was determined by Quant-iT RNA assay kit (Invitrogen, Eugene, OR) with the Qubit fluorimeter. .. RNA samples with RNA Integrity Numbers (RIN) of 6 or higher were used for cDNA synthesis and real-time PCR arrays. cDNA synthesis was performed using the RT2 First Strand cDNA Kit (SABiosciences, Frederick, MD).

    Microarray:

    Article Title: Elevation of MiR-9–3p suppresses the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via down-regulating FN1, ITGB1 and ITGAV
    Article Snippet: Paragraph title: Microarray analysis and quantitative real time pcr (RT-qPCR) ... The Quant-iT RNA Assay Kit (Molecular Probes, Eugene, OR, USA) was used to detect the RNA concentration.

    Incubation:

    Article Title: Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription
    Article Snippet: The mixture was incubated at 37 °C for 1 h, and consequently the polymerized RNA transcripts could be generated. .. The concentration of prepared RNA transcripts was measured using Quant-iT RNA Assay Kit (Invitrogen).

    Activity Assay:

    Article Title: Targeting of Extracellular RNA Reduces Edema Formation and Infarct Size and Improves Survival After Myocardial Infarction in Mice
    Article Snippet: Total RNA of murine blood samples was quantified in platelet‐free plasma using a GeneQuant photometer (Amersham Pharmacia). .. RNAse activity was measured using the Quant‐iT RNA Assay Kit (Invitrogen). .. Data were analyzed with Prism 6.01 (GraphPad Software).

    Expressing:

    Article Title: Dysregulation of Mesenchymal Stromal Cell Antioxidant Responses in Progressive Multiple Sclerosis
    Article Snippet: RNA was extracted and cDNA produced using the Taqman gene expression cells‐Ct‐kit (Applied Biosystems USA) according to the manufacturer's instructions. .. RNA samples were quantified using a Quant‐iT RNA assay kit (Invitrogen) according to manufacturer's instructions to ensure equal loading.

    Article Title: Cytokine therapy‐mediated neuroprotection in a Friedreich's ataxia mouse model
    Article Snippet: Paragraph title: Gene Expression Analysis: Quantitative Polymerase Chain Reaction ... All RNA samples were quantified using a Qubit Fluorometer and Quant‐iT RNA assay kit (Invitrogen, Paisley, UK), according to manufacturer instructions.

    Article Title: Circulating Plasma Extracellular Vesicles from Septic Mice Induce Inflammation via MicroRNA- and TLR7-Dependent Mechanisms
    Article Snippet: RNA pellets were resuspended in 14 μl of RNase-free water and quantified using Quant-iT RNA Assay Kit (Invitrogen, Waltham, MA) as reported previously ( , ). .. Twelve microliters of RNA solution were used for reverse transcription, according to the protocol of miScript RT Kit (QIAGEN). miRNA expression was quantified using a miScript SYBR Green PCR Kit (QIAGEN).

    Article Title: Imatinib Affects the Expression of SLC22A1 in a Non-Linear Concentration-Dependent Manner Within 24 Hours
    Article Snippet: Paragraph title: Determination of SLC22A1 expression ... The concentration of extracted RNA was determined using the Quant-iT RNA Assay Kit according to manufacturer’s instructions (Invitrogen).

    Article Title: Elevation of MiR-9–3p suppresses the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via down-regulating FN1, ITGB1 and ITGAV
    Article Snippet: The Quant-iT RNA Assay Kit (Molecular Probes, Eugene, OR, USA) was used to detect the RNA concentration. .. Taqman Human MicroRNA Arrays V2.0 (Life Technology, Waltham, MA, USA) was used to measure the expression of microRNAs and U6 was regarded as internal control.

    Ligation:

    Article Title: Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription
    Article Snippet: We added T4 DNA ligase (Promega, USA) and ligation buffer (30 mM Tris-HCl (pH 7.8), 10 mM MgCl2 , 10 mM DTT and 1 mM ATP) containing additional 5 mM ATP to the resulting hybrid solution, and connected the nick of circular DNA/T7 promoter hybrids at 16 °C for 12 h. The closed circular DNA/T7 promoter hybrids (0.5 μM) were mixed with T7 RNA polymerase (5 U μl−1 ) in the reaction buffer (4 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , 1 mM DTT and 0.2 mM spermidine) containing 2 mM rNTP (ribonucleotide solution mix, NEB) and 1 U μl−1 RNase inhibitor in the final concentration; the total reaction volume was 20 μl. .. The concentration of prepared RNA transcripts was measured using Quant-iT RNA Assay Kit (Invitrogen).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: CD4+ Virtual Memory: Antigen-inexperienced T cells reside in the naïve, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity
    Article Snippet: Total RNA was isolated using the Qiagen RNEasy Plus Kit (Qiagen) and treated with RNA-free DNase I (Ambion) to remove genomic DNA. .. RNA concentrations were quantified using a Qubit® Fluorometer and the Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized by reverse transcription using the SuperScript® III First- Strand Synthesis System for RT-PCR (Invitrogen) according to the manufacturer’s instructions. .. TCR CDR3 spectrotyping analysis was performed as has been described in detail elsewhere [ ].

    Generated:

    Article Title: Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription
    Article Snippet: The mixture was incubated at 37 °C for 1 h, and consequently the polymerized RNA transcripts could be generated. .. The concentration of prepared RNA transcripts was measured using Quant-iT RNA Assay Kit (Invitrogen).

    Sequencing:

    Article Title: A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder
    Article Snippet: Paragraph title: Transcriptome sequencing ... The yield of fragmented RNA was quantitated using the Quant-iT RNA assay kit (Invitrogen, Carlsbad, CA, USA).

    Article Title: A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA
    Article Snippet: This specific synthetic RNA sequence section can be detected only by a control probe ( , VEEV-Coprobe). .. The RNA concentration was estimated with the Quant-It™ RNA Assay Kit, Broad-Range (Invitrogen).

    Recombinant:

    Article Title: Cytokine therapy‐mediated neuroprotection in a Friedreich's ataxia mouse model
    Article Snippet: Total RNA was extracted from tissue lysates on ice using the PARIS kit (Ambion), according to manufacturer's instructions, and treated with DNase I recombinant (Roche Diagnostics, Indianapolic, IN)/MgCl2 solution (Bioline, London, UK). .. All RNA samples were quantified using a Qubit Fluorometer and Quant‐iT RNA assay kit (Invitrogen, Paisley, UK), according to manufacturer instructions.

    Article Title: Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana
    Article Snippet: The synthesized products were suspended at a final concentration of 0.1 µg µL−1 in RNase-free water supplemented with Recombinant RNasin (ribonuclease inhibitor; Promega) and stored at −20°C until used ( ). .. The cRNA was quantified by fluorescence using a Quant-iT RNA assay kit (Invitrogen).

    Molecular Weight:

    Article Title: Absolute measurement of gene transcripts with Selfie-digital PCR
    Article Snippet: The concentration of DNA was converted to copy number by taking into account the length of the construct (4321 base pairs) and the 650-Da mean molecular weight of the deoxyribonucleotide monophosphate pair. .. The full-length transcript was gel-purified and quantified using a Quant-iT™ RNA Assay Kit (Q33140 Thermo-Fisher Scientific).

    RNA Sequencing Assay:

    Article Title: A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder
    Article Snippet: RNA-Seq analysis were conducted using total RNA samples from two controls and two patients (P1 and P3) isolated with Trizol (Gibco). .. The yield of fragmented RNA was quantitated using the Quant-iT RNA assay kit (Invitrogen, Carlsbad, CA, USA).

    Fluorescence:

    Article Title: Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana
    Article Snippet: The synthesized products were suspended at a final concentration of 0.1 µg µL−1 in RNase-free water supplemented with Recombinant RNasin (ribonuclease inhibitor; Promega) and stored at −20°C until used ( ). .. The cRNA was quantified by fluorescence using a Quant-iT RNA assay kit (Invitrogen). .. Agarose gel electrophoresis and GelRed (BioAmerica Biotech) staining were used to verify the absence of unincorporated nucleotides in the cRNA.

    Isolation:

    Article Title: CD4+ Virtual Memory: Antigen-inexperienced T cells reside in the naïve, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity
    Article Snippet: Paragraph title: 2.7. RNA Isolation and cDNA synthesis. ... RNA concentrations were quantified using a Qubit® Fluorometer and the Quant-iT RNA assay kit (Invitrogen). cDNA was synthesized by reverse transcription using the SuperScript® III First- Strand Synthesis System for RT-PCR (Invitrogen) according to the manufacturer’s instructions.

    Article Title: A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder
    Article Snippet: RNA-Seq analysis were conducted using total RNA samples from two controls and two patients (P1 and P3) isolated with Trizol (Gibco). .. The yield of fragmented RNA was quantitated using the Quant-iT RNA assay kit (Invitrogen, Carlsbad, CA, USA).

    Article Title: Exosomal microRNAs in seminal plasma are markers of the origin of azoospermia and can predict the presence of sperm in testicular tissue
    Article Snippet: Paragraph title: Small RNA-containing total RNA isolation ... RNA concentration was calculated by using the QUBIT fluorometer and the Quant-iT RNA Assay kit (Invitrogen; CA, USA).

    Article Title: Ketamine Inhalation Ameliorates Ovalbumin-Induced Murine Asthma by Suppressing the Epithelial-Mesenchymal Transition
    Article Snippet: Total RNA was isolated using a miRNeasy Kit (Qiagen, Valencia, California, USA) according to the manufacturer’s instructions. .. Quality-confirmed total RNA samples were determined with the Quant-iT™ RNA Assay kit (Invitrogen, Carlsbad, California, USA).

    Article Title: The effects of different molecular weight chondroitin-4-sulfates in chondrocyte pellet culture
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis, and real-time PCR ... In brief, the pellets were washed with PBS, and 1 mL of trizol was added to each sample and homogenized using the PowerGen 125 Tissue Homogenizer for 30–60 s. The trizol mixture was centrifuged, and the supernatant was precipitated using a series of solvents before quantification, which was performed with the Quant-iT™ RNA Assay Kit (Molecular Probes, Eugene, OR, USA).

    Article Title: A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA
    Article Snippet: The plasmid was linearized with Xba I and subsequently transcribed into RNA and the DNA degraded using the Riboprobe® Combination System, T3/T7 RNA Polymerase (Promega Corporation's, Madison, WI, USA), and the QIAamp Viral RNA Mini Kit (Qiagen) was used for RNA isolation (without carrier RNA). .. The RNA concentration was estimated with the Quant-It™ RNA Assay Kit, Broad-Range (Invitrogen).

    Article Title: A Novel Mouse Model of Schistosoma haematobium Egg-Induced Immunopathology
    Article Snippet: RNA was isolated and purified using RNAqueous -Micro kits (Ambion, Austin, TX) according to the manufacturer's instructions. .. The concentration of RNA was determined by Quant-iT RNA assay kit (Invitrogen, Eugene, OR) with the Qubit fluorimeter.

    Article Title: Circulating Plasma Extracellular Vesicles from Septic Mice Induce Inflammation via MicroRNA- and TLR7-Dependent Mechanisms
    Article Snippet: Total RNA was isolated from 250 μl of plasma-derived EVs using miRNeasy Micro Kit (QIAGEN, Germantown, MD). .. RNA pellets were resuspended in 14 μl of RNase-free water and quantified using Quant-iT RNA Assay Kit (Invitrogen, Waltham, MA) as reported previously ( , ).

    Article Title: Diversity and methane oxidation of active epibiotic methanotrophs on live Shinkaia crosnieri
    Article Snippet: Total RNA was extracted from the epibiont communities associated with the dissected setae (stored at −80 °C) using the RNA PowerSoil Total RNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The RNA quantity was determined using the Quant-iT RNA Assay Kit (Life Technologies, Tokyo, Japan).

    Purification:

    Article Title: Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria
    Article Snippet: After centrifugation, pellets were stored at − 80 °C until RNA purification. .. The resulting RNA was quantified using a Qubit 2.0 fluorometer using Life Technologies Quant-iT™ RNA Assay Kit.

    Article Title: A Novel Mouse Model of Schistosoma haematobium Egg-Induced Immunopathology
    Article Snippet: Paragraph title: RNA purification, cDNA synthesis, and real-time PCR ... The concentration of RNA was determined by Quant-iT RNA assay kit (Invitrogen, Eugene, OR) with the Qubit fluorimeter.

    Polymerase Chain Reaction:

    Article Title: The effects of different molecular weight chondroitin-4-sulfates in chondrocyte pellet culture
    Article Snippet: In brief, the pellets were washed with PBS, and 1 mL of trizol was added to each sample and homogenized using the PowerGen 125 Tissue Homogenizer for 30–60 s. The trizol mixture was centrifuged, and the supernatant was precipitated using a series of solvents before quantification, which was performed with the Quant-iT™ RNA Assay Kit (Molecular Probes, Eugene, OR, USA). .. In brief, the pellets were washed with PBS, and 1 mL of trizol was added to each sample and homogenized using the PowerGen 125 Tissue Homogenizer for 30–60 s. The trizol mixture was centrifuged, and the supernatant was precipitated using a series of solvents before quantification, which was performed with the Quant-iT™ RNA Assay Kit (Molecular Probes, Eugene, OR, USA).

    Article Title: A Novel Mouse Model of Schistosoma haematobium Egg-Induced Immunopathology
    Article Snippet: The concentration of RNA was determined by Quant-iT RNA assay kit (Invitrogen, Eugene, OR) with the Qubit fluorimeter. .. RNA samples with RNA Integrity Numbers (RIN) of 6 or higher were used for cDNA synthesis and real-time PCR arrays. cDNA synthesis was performed using the RT2 First Strand cDNA Kit (SABiosciences, Frederick, MD).

    Construct:

    Article Title: Absolute measurement of gene transcripts with Selfie-digital PCR
    Article Snippet: The concentration of DNA was converted to copy number by taking into account the length of the construct (4321 base pairs) and the 650-Da mean molecular weight of the deoxyribonucleotide monophosphate pair. .. The full-length transcript was gel-purified and quantified using a Quant-iT™ RNA Assay Kit (Q33140 Thermo-Fisher Scientific).

    Plasmid Preparation:

    Article Title: A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA
    Article Snippet: The plasmid was linearized with Xba I and subsequently transcribed into RNA and the DNA degraded using the Riboprobe® Combination System, T3/T7 RNA Polymerase (Promega Corporation's, Madison, WI, USA), and the QIAamp Viral RNA Mini Kit (Qiagen) was used for RNA isolation (without carrier RNA). .. The RNA concentration was estimated with the Quant-It™ RNA Assay Kit, Broad-Range (Invitrogen).

    Article Title: Absolute measurement of gene transcripts with Selfie-digital PCR
    Article Snippet: Polyadenylated Gsk3 β RNA of a fixed length was synthesized from linearized vector template by using T7 RNA polymerase (EO0111, Thermo-Fisher Scientific). .. The full-length transcript was gel-purified and quantified using a Quant-iT™ RNA Assay Kit (Q33140 Thermo-Fisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Dysregulation of Mesenchymal Stromal Cell Antioxidant Responses in Progressive Multiple Sclerosis
    Article Snippet: Paragraph title: Real‐Time Polymerase Chain Reaction ... RNA samples were quantified using a Quant‐iT RNA assay kit (Invitrogen) according to manufacturer's instructions to ensure equal loading.

    Article Title: Cytokine therapy‐mediated neuroprotection in a Friedreich's ataxia mouse model
    Article Snippet: Paragraph title: Gene Expression Analysis: Quantitative Polymerase Chain Reaction ... All RNA samples were quantified using a Qubit Fluorometer and Quant‐iT RNA assay kit (Invitrogen, Paisley, UK), according to manufacturer instructions.

    Article Title: The effects of different molecular weight chondroitin-4-sulfates in chondrocyte pellet culture
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis, and real-time PCR ... In brief, the pellets were washed with PBS, and 1 mL of trizol was added to each sample and homogenized using the PowerGen 125 Tissue Homogenizer for 30–60 s. The trizol mixture was centrifuged, and the supernatant was precipitated using a series of solvents before quantification, which was performed with the Quant-iT™ RNA Assay Kit (Molecular Probes, Eugene, OR, USA).

    Article Title: A Novel Mouse Model of Schistosoma haematobium Egg-Induced Immunopathology
    Article Snippet: Paragraph title: RNA purification, cDNA synthesis, and real-time PCR ... The concentration of RNA was determined by Quant-iT RNA assay kit (Invitrogen, Eugene, OR) with the Qubit fluorimeter.

    Article Title: Imatinib Affects the Expression of SLC22A1 in a Non-Linear Concentration-Dependent Manner Within 24 Hours
    Article Snippet: The concentration of extracted RNA was determined using the Quant-iT RNA Assay Kit according to manufacturer’s instructions (Invitrogen). .. The concentration of extracted RNA was determined using the Quant-iT RNA Assay Kit according to manufacturer’s instructions (Invitrogen).

    Article Title: Elevation of MiR-9–3p suppresses the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via down-regulating FN1, ITGB1 and ITGAV
    Article Snippet: Paragraph title: Microarray analysis and quantitative real time pcr (RT-qPCR) ... The Quant-iT RNA Assay Kit (Molecular Probes, Eugene, OR, USA) was used to detect the RNA concentration.

    RNA Extraction:

    Article Title: The gene expression profile of a drug metabolism system and signal transduction pathways in the liver of mice treated with tert-butylhydroquinone or 3-(3'-tert-butyl-4'-hydroxyphenyl)propylthiosulfonate of sodium
    Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... Concentration of RNA was measured using the Quant-iT RNA Assay Kit and a Qubit Fluorometer (Invitrogen).

    Article Title: Circulating Plasma Extracellular Vesicles from Septic Mice Induce Inflammation via MicroRNA- and TLR7-Dependent Mechanisms
    Article Snippet: A spike-in control, Caenorhabditis elegans miRNA (cel-miR-39), was added prior to the RNA extraction. .. RNA pellets were resuspended in 14 μl of RNase-free water and quantified using Quant-iT RNA Assay Kit (Invitrogen, Waltham, MA) as reported previously ( , ).

    Article Title: Elevation of MiR-9–3p suppresses the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via down-regulating FN1, ITGB1 and ITGAV
    Article Snippet: As described previously Total RNA was extracted from frozen NPC tissues or cells by using RNA extraction Kit (Invitrogen, CA, USA). .. The Quant-iT RNA Assay Kit (Molecular Probes, Eugene, OR, USA) was used to detect the RNA concentration.

    Agarose Gel Electrophoresis:

    Article Title: The gene expression profile of a drug metabolism system and signal transduction pathways in the liver of mice treated with tert-butylhydroquinone or 3-(3'-tert-butyl-4'-hydroxyphenyl)propylthiosulfonate of sodium
    Article Snippet: The quality of RNA was tested on a 2% agarose gel. .. Concentration of RNA was measured using the Quant-iT RNA Assay Kit and a Qubit Fluorometer (Invitrogen).

    In Vitro:

    Article Title: Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana
    Article Snippet: Paragraph title: In Vitro RNA Synthesis for Oocyte Assays ... The cRNA was quantified by fluorescence using a Quant-iT RNA assay kit (Invitrogen).

    Spectrophotometry:

    Article Title: Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria
    Article Snippet: The resulting RNA was quantified using a Qubit 2.0 fluorometer using Life Technologies Quant-iT™ RNA Assay Kit. .. The resulting RNA was quantified using a Qubit 2.0 fluorometer using Life Technologies Quant-iT™ RNA Assay Kit.

    Produced:

    Article Title: Dysregulation of Mesenchymal Stromal Cell Antioxidant Responses in Progressive Multiple Sclerosis
    Article Snippet: RNA was extracted and cDNA produced using the Taqman gene expression cells‐Ct‐kit (Applied Biosystems USA) according to the manufacturer's instructions. .. RNA samples were quantified using a Quant‐iT RNA assay kit (Invitrogen) according to manufacturer's instructions to ensure equal loading.

    Concentration Assay:

    Article Title: Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription
    Article Snippet: At the end of RCT reaction, DNase 1 (2 U μl−1 , NEB) was treated for 37 °C for 15 min and then enzymes present in the reaction mixture were inactivated by heating at 90 °C. .. The concentration of prepared RNA transcripts was measured using Quant-iT RNA Assay Kit (Invitrogen). .. We added 5′-amine-modified DNA fragment (10 mM), which was dissolved in conjugation buffer (100 mM MES, 500 mM NaCl, pH 6.0), to Sulfo-NHS (10 mM) and EDC (4 mM) solution, and then reacted the resulting DNA mixture with a 10-fold molar excess of folates for 3 h at 22 °C. β-Mercaptoethanol was added for deactivation of unreacted EDC.

    Article Title: Exosomal microRNAs in seminal plasma are markers of the origin of azoospermia and can predict the presence of sperm in testicular tissue
    Article Snippet: Total RNA was obtained from exosomes using the miRCURY RNA Isolation Kit-Cell and Plant (Exiqon; Denmark). .. RNA concentration was calculated by using the QUBIT fluorometer and the Quant-iT RNA Assay kit (Invitrogen; CA, USA). .. All RNA samples presented a OD 260/280 nm ratio ≥1.7 when using a Nanodrop UV–Vis spectrophotometer (Thermo Fisher Scientific; MA, USA).

    Article Title: A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA
    Article Snippet: The plasmid was linearized with Xba I and subsequently transcribed into RNA and the DNA degraded using the Riboprobe® Combination System, T3/T7 RNA Polymerase (Promega Corporation's, Madison, WI, USA), and the QIAamp Viral RNA Mini Kit (Qiagen) was used for RNA isolation (without carrier RNA). .. The RNA concentration was estimated with the Quant-It™ RNA Assay Kit, Broad-Range (Invitrogen). .. The copy number of the synthetic RNA was calculated from the RNA concentration and the molecular mass of the RNA transcript.

    Article Title: A Novel Mouse Model of Schistosoma haematobium Egg-Induced Immunopathology
    Article Snippet: RNA was isolated and purified using RNAqueous -Micro kits (Ambion, Austin, TX) according to the manufacturer's instructions. .. The concentration of RNA was determined by Quant-iT RNA assay kit (Invitrogen, Eugene, OR) with the Qubit fluorimeter. .. The ribosomal RNA band integrity of each RNA sample was run on an Agilent Bioanalyzer using an RNA 6000 Nano Labchip.

    Article Title: Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana
    Article Snippet: The synthesized products were suspended at a final concentration of 0.1 µg µL−1 in RNase-free water supplemented with Recombinant RNasin (ribonuclease inhibitor; Promega) and stored at −20°C until used ( ). .. The cRNA was quantified by fluorescence using a Quant-iT RNA assay kit (Invitrogen).

    Article Title: The gene expression profile of a drug metabolism system and signal transduction pathways in the liver of mice treated with tert-butylhydroquinone or 3-(3'-tert-butyl-4'-hydroxyphenyl)propylthiosulfonate of sodium
    Article Snippet: The A260 /A280 ratio of RNA was 1.8–2.0. .. Concentration of RNA was measured using the Quant-iT RNA Assay Kit and a Qubit Fluorometer (Invitrogen). .. Next, 1 μg of RNA was reverse-transcribed using the ABI High Capacity RNA to cDNA kit (Applied Biosystems) according to the manufacturer's instructions.

    Article Title: Imatinib Affects the Expression of SLC22A1 in a Non-Linear Concentration-Dependent Manner Within 24 Hours
    Article Snippet: Total RNA was extracted from K562 cells using TRI reagent (Sigma-Aldrich) according to the manufacturer’s instructions. .. The concentration of extracted RNA was determined using the Quant-iT RNA Assay Kit according to manufacturer’s instructions (Invitrogen). .. A standard amount of 2 μg of total RNA was reverse-transcribed using the High Capacity RNA-to-cDNA Kit (Applied Biosystems) in a final volume of 20 μL according to the manufacturer’s instructions.

    Article Title: Elevation of MiR-9–3p suppresses the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via down-regulating FN1, ITGB1 and ITGAV
    Article Snippet: As described previously Total RNA was extracted from frozen NPC tissues or cells by using RNA extraction Kit (Invitrogen, CA, USA). .. The Quant-iT RNA Assay Kit (Molecular Probes, Eugene, OR, USA) was used to detect the RNA concentration. .. Taqman Human MicroRNA Arrays V2.0 (Life Technology, Waltham, MA, USA) was used to measure the expression of microRNAs and U6 was regarded as internal control.

    Article Title: Absolute measurement of gene transcripts with Selfie-digital PCR
    Article Snippet: The concentration of DNA was converted to copy number by taking into account the length of the construct (4321 base pairs) and the 650-Da mean molecular weight of the deoxyribonucleotide monophosphate pair. .. The full-length transcript was gel-purified and quantified using a Quant-iT™ RNA Assay Kit (Q33140 Thermo-Fisher Scientific).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher quant it ribogreen rna assay kit
    Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ <t>RiboGreen®</t> <t>RNA</t> Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.
    Quant It Ribogreen Rna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it ribogreen rna assay kit/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    quant it ribogreen rna assay kit - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Journal: PLoS ONE

    Article Title: Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes

    doi: 10.1371/journal.pone.0195969

    Figure Lengend Snippet: Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Article Snippet: RNA was quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific).

    Techniques: Transfection, Expressing, Negative Control, Western Blot, Plasmid Preparation, Isolation, Purification, SDS Page, Staining, Pull Down Assay, Incubation