quant it ribogreen assay kit  (Thermo Fisher)


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    Thermo Fisher quant it ribogreen assay kit
    Quant It Ribogreen Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it ribogreen assay kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quant it ribogreen assay kit - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Contrasting diurnal patterns in antioxidant capacities, but not in expression of stress protein genes among copepod populations from clear versus glacially fed alpine and subalpine lakes
    Article Snippet: Extraction of RNA and cDNA synthesis RNA extraction and cDNA synthesis were done as described in detail previously ( ). .. We quantified the RNA concentration in triplicate with a plate reader (2030 Multilabel Plate Reader Victor X4, Perkin Elmer) and the Quant-iT RiboGreen Assay Kit (Life Technologies).

    Agarose Gel Electrophoresis:

    Article Title: Contrasting diurnal patterns in antioxidant capacities, but not in expression of stress protein genes among copepod populations from clear versus glacially fed alpine and subalpine lakes
    Article Snippet: Total RNA was measured (NanoDrop ND1000, Thermo Fisher Scientific) to assess RNA quality (mean A260/280 ratio: 2.10 ± 0.04) and run on a 1.2% agarose gel to assess RNA integrity. .. We quantified the RNA concentration in triplicate with a plate reader (2030 Multilabel Plate Reader Victor X4, Perkin Elmer) and the Quant-iT RiboGreen Assay Kit (Life Technologies).

    Synthesized:

    Article Title: Contrasting diurnal patterns in antioxidant capacities, but not in expression of stress protein genes among copepod populations from clear versus glacially fed alpine and subalpine lakes
    Article Snippet: We quantified the RNA concentration in triplicate with a plate reader (2030 Multilabel Plate Reader Victor X4, Perkin Elmer) and the Quant-iT RiboGreen Assay Kit (Life Technologies). .. Copepod RNA (450 ng) and random hexamer primers (#S0142, Thermo Fisher Scientific) were used for first-strand cDNA synthesis. cDNA was synthesized using MMLV H minus reverse transcriptase (#EP0452, Thermo Fisher Scientific) following the manufacturer’s protocol with slight modifications by using less reverse transcriptase (100 U in 50 μL reaction volume).

    Purification:

    Article Title: Contrasting diurnal patterns in antioxidant capacities, but not in expression of stress protein genes among copepod populations from clear versus glacially fed alpine and subalpine lakes
    Article Snippet: The RNA extract was further purified (RNeasy Mini Kit, Qiagen; according the manufacturer’s protocol). .. We quantified the RNA concentration in triplicate with a plate reader (2030 Multilabel Plate Reader Victor X4, Perkin Elmer) and the Quant-iT RiboGreen Assay Kit (Life Technologies).

    Real-time Polymerase Chain Reaction:

    Article Title: Contrasting diurnal patterns in antioxidant capacities, but not in expression of stress protein genes among copepod populations from clear versus glacially fed alpine and subalpine lakes
    Article Snippet: We quantified the RNA concentration in triplicate with a plate reader (2030 Multilabel Plate Reader Victor X4, Perkin Elmer) and the Quant-iT RiboGreen Assay Kit (Life Technologies). .. Before quantitative polymerase chain reaction (qPCR) analysis, cDNA was frozen at −20°C.

    Concentration Assay:

    Article Title: Contrasting diurnal patterns in antioxidant capacities, but not in expression of stress protein genes among copepod populations from clear versus glacially fed alpine and subalpine lakes
    Article Snippet: .. We quantified the RNA concentration in triplicate with a plate reader (2030 Multilabel Plate Reader Victor X4, Perkin Elmer) and the Quant-iT RiboGreen Assay Kit (Life Technologies). .. Copepod RNA (450 ng) and random hexamer primers (#S0142, Thermo Fisher Scientific) were used for first-strand cDNA synthesis. cDNA was synthesized using MMLV H minus reverse transcriptase (#EP0452, Thermo Fisher Scientific) following the manufacturer’s protocol with slight modifications by using less reverse transcriptase (100 U in 50 μL reaction volume).

    Random Hexamer Labeling:

    Article Title: Contrasting diurnal patterns in antioxidant capacities, but not in expression of stress protein genes among copepod populations from clear versus glacially fed alpine and subalpine lakes
    Article Snippet: We quantified the RNA concentration in triplicate with a plate reader (2030 Multilabel Plate Reader Victor X4, Perkin Elmer) and the Quant-iT RiboGreen Assay Kit (Life Technologies). .. Copepod RNA (450 ng) and random hexamer primers (#S0142, Thermo Fisher Scientific) were used for first-strand cDNA synthesis. cDNA was synthesized using MMLV H minus reverse transcriptase (#EP0452, Thermo Fisher Scientific) following the manufacturer’s protocol with slight modifications by using less reverse transcriptase (100 U in 50 μL reaction volume).

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    Thermo Fisher quant it ribogreen assay kit
    Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) <t>Ribogreen</t> assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p
    Quant It Ribogreen Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it ribogreen assay kit/product/Thermo Fisher
    Average 99 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    quant it ribogreen assay kit - by Bioz Stars, 2020-03
    99/100 stars
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    Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) Ribogreen assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p

    Journal: ACS nano

    Article Title: Zwitterionic Nanocarrier Surface Chemistry Improves siRNA Tumor Delivery and Silencing Activity Relative to Polyethylene Glycol

    doi: 10.1021/acsnano.7b01110

    Figure Lengend Snippet: Polyplexes with different corona chemistries have similar size, zeta potential, and cargo loading but varied stability against high salt concentrations. (A, B) Polyplex siRNA encapsulation efficiency and stability is highest at N + :P − 20. (A) Ribogreen assay reveals polyplex encapsulation plateaus by N + :P − ratio of 10. (B) Polyplexes retain higher stability after a 30 min incubation in 30% FBS at N + :P − 20 compared to N + :P − 10 ( p

    Article Snippet: Polyplex encapsulation efficiency at various N+ :P− ratios was evaluated using a Quant-iT Ribogreen assay kit (ThermoFisher, USA).

    Techniques: Incubation

    Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Journal: PLoS ONE

    Article Title: Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes

    doi: 10.1371/journal.pone.0195969

    Figure Lengend Snippet: Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Article Snippet: RNA was quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific).

    Techniques: Transfection, Expressing, Negative Control, Western Blot, Plasmid Preparation, Isolation, Purification, SDS Page, Staining, Pull Down Assay, Incubation

    Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Journal: PLoS ONE

    Article Title: Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes

    doi: 10.1371/journal.pone.0195969

    Figure Lengend Snippet: Validation of MVP. Transfected cells are expressing MVP-biotin and un-transfected cells were used as negative control. (A) Western blot analysis for the detection of MVP in HEK293F cell lysate and exosomal protein extracts shows that plasmid (MVP-biotin) was successfully expressed in HEK293F cells and was partitioned into exosomes isolated from these transfected cells. ( B ) Protein samples from different stages of MVP purification were separated by SDS-PAGE and the protein bands were detected by Comassie staining. After the pull down assay, the presence of MVP within exosomes was confirmed, i.e. MVP in exosomes from biotinylated MVP-transfected cells PD (pull-down lane), whereas it was absent in exosomes from untransfected cells. The position of the MVP-biotin band is indicated by squares. A representative experiment out of 3 is shown. ( C ) Total amount of RNAs coupled to MVP were extracted and quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific). Captured RNA was expressed as a percentage of RNA eluted from the beads after the pull-down respect to the total RNA incubated with the beads. Exosomal-MVP was coupled with RNAs and the quantification of RNAs that had co-eluted with MVP showed that the amount of RNA present in the MVP elute (i.e. from MVP-RNA complex) was significantly higher than that from the untransfected control. The experiment was performed in 3-replicates and the graph shows the range of values and median (grey). Average and standard error of three independent experiments are shown. Samples: W = proteins from different wash steps during the MVP purification, and PD = pull-down proteins (MVP) after elution, I = input, and UB = unbound after RBP capture.

    Article Snippet: RNA was quantified by Quant-iT™ RiboGreen® RNA Assay Kit (ThermoFisher Scientific).

    Techniques: Transfection, Expressing, Negative Control, Western Blot, Plasmid Preparation, Isolation, Purification, SDS Page, Staining, Pull Down Assay, Incubation