quant it picogreen assay kit  (Thermo Fisher)


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    Name:
    Quant iT PicoGreen dsDNA Assay Kit
    Description:
    Using the PicoGreen dsDNA quantitation assay you can selectively detect as little as 25 pg ml of dsDNA in the presence of ssDNA RNA and free nucleotides The assay is linear over three orders of magnitude and has little sequence dependence allowing you to accurately measure DNA from many sources including genomic DNA viral DNA miniprep DNA or PCR amplification products PicoGreen dsDNA Quantitation Reagents are • Orders of magnitude more sensitive than UV absorbance readings saving on precious sample Figure 1 • Specific for dsDNA in the presence of equimolar amounts of RNA Figure 2 • Easy to use just add the dye to sample wait 5 minutes and read• Suitable for use in 96 and 384 well plate formats• Compatible with most fluorescence based microplate readers and fluorometers and FluorescenceApplications The PicoGreen dsDNA Quantitation Reagent and Kits are ideal for PCR based assays microarray samples DNA damage assays enzyme activity assays genomic DNA quantitation measuring dsDNA in complex mixtures and viral DNA quantitation
    Catalog Number:
    p11496
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Quantitation|Nucleic Acid Quantitation
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher quant it picogreen assay kit
    DNA quantity per scaffold at 3 and 7 days, assessed by <t>PicoGreen</t> assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points
    Using the PicoGreen dsDNA quantitation assay you can selectively detect as little as 25 pg ml of dsDNA in the presence of ssDNA RNA and free nucleotides The assay is linear over three orders of magnitude and has little sequence dependence allowing you to accurately measure DNA from many sources including genomic DNA viral DNA miniprep DNA or PCR amplification products PicoGreen dsDNA Quantitation Reagents are • Orders of magnitude more sensitive than UV absorbance readings saving on precious sample Figure 1 • Specific for dsDNA in the presence of equimolar amounts of RNA Figure 2 • Easy to use just add the dye to sample wait 5 minutes and read• Suitable for use in 96 and 384 well plate formats• Compatible with most fluorescence based microplate readers and fluorometers and FluorescenceApplications The PicoGreen dsDNA Quantitation Reagent and Kits are ideal for PCR based assays microarray samples DNA damage assays enzyme activity assays genomic DNA quantitation measuring dsDNA in complex mixtures and viral DNA quantitation
    https://www.bioz.com/result/quant it picogreen assay kit/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    quant it picogreen assay kit - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering"

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering

    Journal: Tissue Engineering and Regenerative Medicine

    doi: 10.1007/s13770-017-0107-5

    DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points
    Figure Legend Snippet: DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points

    Techniques Used: Picogreen Assay

    2) Product Images from "Osteogenic Responses to Zirconia with Hydroxyapatite Coating by Aerosol Deposition"

    Article Title: Osteogenic Responses to Zirconia with Hydroxyapatite Coating by Aerosol Deposition

    Journal: Journal of Dental Research

    doi: 10.1177/0022034514566432

    Cellular proliferation and viability on the discs. ( A ) Picogreen assay. Proliferation of MC3T3-E1 cells on titanium (Ti) or zirconia (Zr) discs on days 1, 4, and 7. ( B ) CCK-8 assay. Cell viability was tested on days 1, 4, and 7 under the same conditions
    Figure Legend Snippet: Cellular proliferation and viability on the discs. ( A ) Picogreen assay. Proliferation of MC3T3-E1 cells on titanium (Ti) or zirconia (Zr) discs on days 1, 4, and 7. ( B ) CCK-8 assay. Cell viability was tested on days 1, 4, and 7 under the same conditions

    Techniques Used: Picogreen Assay, CCK-8 Assay

    Related Articles

    Picogreen Assay:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: .. Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions. ..

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: .. DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. PCR was performed with 5 ng of isolated DNA, AmpliTaq Gold 360 (Invitrogen) and 0.5 µM of the following primers: mtDNA encoded NADH dehydrogenase subunit 1 (ND1; 5′-GCATTCCTAATGCTTACCGAAC-3′ and 5′-AAGGGTGGAGAGGTTAAAGGAG-3′); genomic DNA encoded Glyceraldehyde 3- phosphate dehydrogenase (GAPDH; 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-TTGATTTTGGAGGGATCTCG-3′).

    Article Title: Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell
    Article Snippet: .. DNA Quantification Double-stranded DNA contamination was assessed in purified vectors using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen). .. The manufacturers’ protocol was followed using a Thermo Scientific Nunc F96 MicroWell black polystyrene plate.

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions. ..

    Article Title: Kidney Decellularized Extracellular Matrix Hydrogels: Rheological Characterization and Human Glomerular Endothelial Cell Response to Encapsulation
    Article Snippet: .. DNA was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, #P7589) following the manufacturer’s protocol and a dsDNA standard included in the kit to generate a standard curve. .. Fluorescence was measured on a BioTek Cytation 3 Multi-Mode Reader with an excitation wavelength of 480 nm and emission wavelength of 520 nm.

    Article Title: Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality
    Article Snippet: .. For quantification, DNA was extracted using the PureLink™ genomic DNA mini kit (Thermo Fisher, Ghent, Belgium, K1820-01) and quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher, P7589) following the manufacturer’s instructions. .. Preservation of fibronectin, collagen IV and laminin were evaluated by immunohistochemistry.

    Article Title: Collagen and Hydroxyapatite Scaffolds Activate Distinct Osteogenesis Signaling Pathways in Adult Adipose-Derived Multipotent Stromal Cells
    Article Snippet: .. Total dsDNA was quantified using a commercially available kit (Quant-iT PicoGreen dsDNA Assay Kit; Thermo Fisher Scientific). .. Fluorescence was measured at 480 and 520 nm and dsDNA quantified on a lambda DNA standard curve.

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: .. Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions. .. Fluorescence was read using a microplate reader at Ex 490 nm Em 510–570 nm, N = 4 independent replicates.

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds
    Article Snippet: .. The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific. .. RNase-free DNase was obtained from Qiagen.

    Article Title: Histologic Characterization of Acellular Dermal Matrices in a Porcine Model of Tissue Expander Breast Reconstruction
    Article Snippet: .. The degree of decellularization was assessed for three lots of AlloDerm Regenerative Tissue Matrix (AlloDerm; LifeCell Corp., Branchburg, NJ) and three lots of AlloMax Surgical Graft [AlloMax; C. R. Bard, Inc. (Davol), Warwick, RI] by three previously established criteria : (i) the presence or absence of intact cells and nuclei on hematoxylin and eosin (H & E)-stained sections and 4′,6-diamidino-2-phenylindole (DAPI)-stained sections, respectively; (ii) the Quant-iT PicoGreen assay (Invitrogen, Carlsbad, CA) for quantification of double-stranded DNA (dsDNA); and (iii) evaluation of samples by agarose gel (2%) electrophoresis to determine the size of remaining DNA fragments. ..

    Article Title: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)
    Article Snippet: .. Therefore extracellular DNA was quantified in the supernatant using a Quant-iT™ Picogreen® dsDNA Assay Kit (Invitrogen) according to the manufacturer's instructions and was analyzed with a SpectraFluor Plus absorbance reader (Tecan Group, Männedorf, Switzerland). ..

    Lambda DNA Preparation:

    Article Title: Collagen and Hydroxyapatite Scaffolds Activate Distinct Osteogenesis Signaling Pathways in Adult Adipose-Derived Multipotent Stromal Cells
    Article Snippet: Total dsDNA was quantified using a commercially available kit (Quant-iT PicoGreen dsDNA Assay Kit; Thermo Fisher Scientific). .. Fluorescence was measured at 480 and 520 nm and dsDNA quantified on a lambda DNA standard curve.

    Electrophoresis:

    Article Title: Histologic Characterization of Acellular Dermal Matrices in a Porcine Model of Tissue Expander Breast Reconstruction
    Article Snippet: .. The degree of decellularization was assessed for three lots of AlloDerm Regenerative Tissue Matrix (AlloDerm; LifeCell Corp., Branchburg, NJ) and three lots of AlloMax Surgical Graft [AlloMax; C. R. Bard, Inc. (Davol), Warwick, RI] by three previously established criteria : (i) the presence or absence of intact cells and nuclei on hematoxylin and eosin (H & E)-stained sections and 4′,6-diamidino-2-phenylindole (DAPI)-stained sections, respectively; (ii) the Quant-iT PicoGreen assay (Invitrogen, Carlsbad, CA) for quantification of double-stranded DNA (dsDNA); and (iii) evaluation of samples by agarose gel (2%) electrophoresis to determine the size of remaining DNA fragments. ..

    Incubation:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: Upon completion of the incubation, scaffolds were spun down, and supernatants were collected and subjected to DNA, GAG, and Hydroxyproline content measurements. .. Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions.

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: DNA quantification Scaffolds were washed 3 times in PBS and freeze dried for 24 h. Each scaffold was incubated within an ultrasonic bath (6 litre Cavitek Digital, The Allendale Group, UK) in 300 µl of a 2.5 units/ml papain digest solution, 5 mM cysteine HCL and 5 mM EDTA in PBS (all reagents from Sigma Aldrich, UK) at 60 °C for 24 h with periodic sonication. .. Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions.

    Article Title: Pullulan: a new cytoadhesive for cell-mediated cartilage repair
    Article Snippet: MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), was applied according to the manufacturer’s instructions (Promega, Southampton, UK) and the culture incubated for two hours at 37°C. .. The Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Molecular Probes, Life Technologies, Paisley, UK) was utilised according to the manufacturer’s protocol.

    Colorimetric Assay:

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: For quantitative comparison, collagen and proteoglycan content were measured using the hydroxyproline assay [ ] for collagen content and the 1,9-dimethylmethylene blue (DMMB) colorimetric assay for proteoglycan content ( n = 6 pellets/group) [ ]. .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. PCR was performed with 5 ng of isolated DNA, AmpliTaq Gold 360 (Invitrogen) and 0.5 µM of the following primers: mtDNA encoded NADH dehydrogenase subunit 1 (ND1; 5′-GCATTCCTAATGCTTACCGAAC-3′ and 5′-AAGGGTGGAGAGGTTAAAGGAG-3′); genomic DNA encoded Glyceraldehyde 3- phosphate dehydrogenase (GAPDH; 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-TTGATTTTGGAGGGATCTCG-3′).

    Microplate Reader Absorbance Measurement:

    Article Title: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)
    Article Snippet: .. Therefore extracellular DNA was quantified in the supernatant using a Quant-iT™ Picogreen® dsDNA Assay Kit (Invitrogen) according to the manufacturer's instructions and was analyzed with a SpectraFluor Plus absorbance reader (Tecan Group, Männedorf, Switzerland). ..

    Hydroxyproline Assay:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions. .. Hydroxyproline content was measured on the papain-digested supernatant accordingly using Hydroxyproline Assay Kit (Sigma) according to manufacturer’s instructions.

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: For quantitative comparison, collagen and proteoglycan content were measured using the hydroxyproline assay [ ] for collagen content and the 1,9-dimethylmethylene blue (DMMB) colorimetric assay for proteoglycan content ( n = 6 pellets/group) [ ]. .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions.

    Transmission Electron Microscopy:

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds
    Article Snippet: The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific. .. All reagents were assessed by TEM to ensure that they were free of contaminating EVs.

    Fluorescence:

    Article Title: Kidney Decellularized Extracellular Matrix Hydrogels: Rheological Characterization and Human Glomerular Endothelial Cell Response to Encapsulation
    Article Snippet: DNA was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, #P7589) following the manufacturer’s protocol and a dsDNA standard included in the kit to generate a standard curve. .. Fluorescence was measured on a BioTek Cytation 3 Multi-Mode Reader with an excitation wavelength of 480 nm and emission wavelength of 520 nm.

    Article Title: Collagen and Hydroxyapatite Scaffolds Activate Distinct Osteogenesis Signaling Pathways in Adult Adipose-Derived Multipotent Stromal Cells
    Article Snippet: Total dsDNA was quantified using a commercially available kit (Quant-iT PicoGreen dsDNA Assay Kit; Thermo Fisher Scientific). .. Fluorescence was measured at 480 and 520 nm and dsDNA quantified on a lambda DNA standard curve.

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions. .. Fluorescence was read using a microplate reader at Ex 490 nm Em 510–570 nm, N = 4 independent replicates.

    Irradiation:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. For dot blot assay, 5 ng of DNA was blotted on a positively charged nylon membrane using the Bio-Dot Microfiltration System (Bio-Rad Laboratories), and then cross-linked by UV irradiation for 3 min.

    Isolation:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: Paragraph title: Isolation of DNA from supernatants and analysis ... DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen).

    Microscopy:

    Article Title: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)
    Article Snippet: NETs were stained with Sytox Green 5 mM (Molecular Probes, Eugene, OR, USA) and then visualized using a confocal microscope (Olympus IX81, Olympus Corporation, Tokyo, Japan). .. Therefore extracellular DNA was quantified in the supernatant using a Quant-iT™ Picogreen® dsDNA Assay Kit (Invitrogen) according to the manufacturer's instructions and was analyzed with a SpectraFluor Plus absorbance reader (Tecan Group, Männedorf, Switzerland).

    Purification:

    Article Title: Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell
    Article Snippet: .. DNA Quantification Double-stranded DNA contamination was assessed in purified vectors using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen). .. The manufacturers’ protocol was followed using a Thermo Scientific Nunc F96 MicroWell black polystyrene plate.

    Dot Blot:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. For dot blot assay, 5 ng of DNA was blotted on a positively charged nylon membrane using the Bio-Dot Microfiltration System (Bio-Rad Laboratories), and then cross-linked by UV irradiation for 3 min.

    Sonication:

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: DNA quantification Scaffolds were washed 3 times in PBS and freeze dried for 24 h. Each scaffold was incubated within an ultrasonic bath (6 litre Cavitek Digital, The Allendale Group, UK) in 300 µl of a 2.5 units/ml papain digest solution, 5 mM cysteine HCL and 5 mM EDTA in PBS (all reagents from Sigma Aldrich, UK) at 60 °C for 24 h with periodic sonication. .. Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions.

    Software:

    Article Title: Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell
    Article Snippet: DNA Quantification Double-stranded DNA contamination was assessed in purified vectors using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen). .. Absorbance was measured using the Varioskan LUX multimode microplate reader and analyzed using the SkanIt software.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)
    Article Snippet: Since NET formation is quantified by the measurement of DNA release after DNase treatment, and DNA will degrade the epitope of the anti-nucleosome antibody, measurement of nucleosomes by this ELISA is not suitable [ ]. .. Therefore extracellular DNA was quantified in the supernatant using a Quant-iT™ Picogreen® dsDNA Assay Kit (Invitrogen) according to the manufacturer's instructions and was analyzed with a SpectraFluor Plus absorbance reader (Tecan Group, Männedorf, Switzerland).

    Agarose Gel Electrophoresis:

    Article Title: Histologic Characterization of Acellular Dermal Matrices in a Porcine Model of Tissue Expander Breast Reconstruction
    Article Snippet: .. The degree of decellularization was assessed for three lots of AlloDerm Regenerative Tissue Matrix (AlloDerm; LifeCell Corp., Branchburg, NJ) and three lots of AlloMax Surgical Graft [AlloMax; C. R. Bard, Inc. (Davol), Warwick, RI] by three previously established criteria : (i) the presence or absence of intact cells and nuclei on hematoxylin and eosin (H & E)-stained sections and 4′,6-diamidino-2-phenylindole (DAPI)-stained sections, respectively; (ii) the Quant-iT PicoGreen assay (Invitrogen, Carlsbad, CA) for quantification of double-stranded DNA (dsDNA); and (iii) evaluation of samples by agarose gel (2%) electrophoresis to determine the size of remaining DNA fragments. ..

    In Vitro:

    Article Title: Histologic Characterization of Acellular Dermal Matrices in a Porcine Model of Tissue Expander Breast Reconstruction
    Article Snippet: Paragraph title: In vitro composition analysis ... The degree of decellularization was assessed for three lots of AlloDerm Regenerative Tissue Matrix (AlloDerm; LifeCell Corp., Branchburg, NJ) and three lots of AlloMax Surgical Graft [AlloMax; C. R. Bard, Inc. (Davol), Warwick, RI] by three previously established criteria : (i) the presence or absence of intact cells and nuclei on hematoxylin and eosin (H & E)-stained sections and 4′,6-diamidino-2-phenylindole (DAPI)-stained sections, respectively; (ii) the Quant-iT PicoGreen assay (Invitrogen, Carlsbad, CA) for quantification of double-stranded DNA (dsDNA); and (iii) evaluation of samples by agarose gel (2%) electrophoresis to determine the size of remaining DNA fragments.

    dsDNA Assay:

    Article Title: Pullulan: a new cytoadhesive for cell-mediated cartilage repair
    Article Snippet: .. The Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Molecular Probes, Life Technologies, Paisley, UK) was utilised according to the manufacturer’s protocol. .. Samples were evaluated on a Wallac Victor™ 1420 Multilabel Counter spectrophotometer.

    Spectrophotometry:

    Article Title: Pullulan: a new cytoadhesive for cell-mediated cartilage repair
    Article Snippet: Culture media absorbance was evaluated at 490 nm on a Wallac Victor™ 1420 Multilabel Counter spectrophotometer. .. The Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Molecular Probes, Life Technologies, Paisley, UK) was utilised according to the manufacturer’s protocol.

    Concentration Assay:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: .. DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. PCR was performed with 5 ng of isolated DNA, AmpliTaq Gold 360 (Invitrogen) and 0.5 µM of the following primers: mtDNA encoded NADH dehydrogenase subunit 1 (ND1; 5′-GCATTCCTAATGCTTACCGAAC-3′ and 5′-AAGGGTGGAGAGGTTAAAGGAG-3′); genomic DNA encoded Glyceraldehyde 3- phosphate dehydrogenase (GAPDH; 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-TTGATTTTGGAGGGATCTCG-3′).

    Staining:

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: The pellets were subjected to macrophotography and wet weight measurement ( n = 12 pellets/group).The pellets were then used for picrosirius red staining to visualize collagen, and for safranin-O staining to assess proteoglycan deposition ( n = 6 pellets/group). .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions.

    Article Title: Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality
    Article Snippet: Sections of native tissue and DT were stained with H and E or Masson’s trichrome to evaluate the presence of nuclear residues and general tissue morphology. .. For quantification, DNA was extracted using the PureLink™ genomic DNA mini kit (Thermo Fisher, Ghent, Belgium, K1820-01) and quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher, P7589) following the manufacturer’s instructions.

    Article Title: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)
    Article Snippet: NETs were stained with Sytox Green 5 mM (Molecular Probes, Eugene, OR, USA) and then visualized using a confocal microscope (Olympus IX81, Olympus Corporation, Tokyo, Japan). .. Therefore extracellular DNA was quantified in the supernatant using a Quant-iT™ Picogreen® dsDNA Assay Kit (Invitrogen) according to the manufacturer's instructions and was analyzed with a SpectraFluor Plus absorbance reader (Tecan Group, Männedorf, Switzerland).

    Dimethylmethylene Blue Assay:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions. .. Total GAG content was determined with DMMB assay on papain-digested supernatant according to the protocol described elsewhere and quantified based on shark chondroitin sulfate standard.

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  • 99
    Thermo Fisher quant it picogreen dsdna assay kit
    DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ <t>PicoGreen</t> ® <t>dsDNA</t> assay. The results are presented as means ± SD. *** p
    Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it picogreen dsdna assay kit/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    quant it picogreen dsdna assay kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    80
    Thermo Fisher quant itã cents ⠄ â cents picogreenã cents ⠄ â cents dsdna assay kit
    Antiproliferative activity of barbatolic acid on HUVEC, HCC1428 and T-47D cell lines. a AlamarBlue™ cell viability assay, b lactate dehydrogenase (LDH) cellular membrane degradation assay and c <t>PicoGreen™</t> <t>dsDNA</t> quantitation assay. Data
    Quant Itã Cents ⠄ â Cents Picogreenã Cents ⠄ â Cents Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant itã cents ⠄ â cents picogreenã cents ⠄ â cents dsdna assay kit/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quant itã cents ⠄ â cents picogreenã cents ⠄ â cents dsdna assay kit - by Bioz Stars, 2020-04
    80/100 stars
      Buy from Supplier

    Image Search Results


    DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ PicoGreen ® dsDNA assay. The results are presented as means ± SD. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality

    doi: 10.3390/ijms19010227

    Figure Lengend Snippet: DNA quantification in DT. ( A ) Nuclei were counted on H and E-stained slides of native tissue and DT and represented as the number of nuclei per µm 2 ; ( B ) Following tissue digestion, DNA was extracted and quantified using the Quant-iT™ PicoGreen ® dsDNA assay. The results are presented as means ± SD. *** p

    Article Snippet: For quantification, DNA was extracted using the PureLink™ genomic DNA mini kit (Thermo Fisher, Ghent, Belgium, K1820-01) and quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher, P7589) following the manufacturer’s instructions.

    Techniques: Staining, Picogreen Assay

    DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points

    Journal: Tissue Engineering and Regenerative Medicine

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering

    doi: 10.1007/s13770-017-0107-5

    Figure Lengend Snippet: DNA quantity per scaffold at 3 and 7 days, assessed by PicoGreen assay. This confirms the ability of all scaffold architectures to support primary kidney cell life. Analysis using a one-way ANOVA showed significant differences F(7,23) = 4.79, p = 0.002, post hoc Tukey analysis shows that was in regards to cryogenic scaffolds. Data presented as mean ± 95% confidence intervals, circles denote individual data points

    Article Snippet: Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions.

    Techniques: Picogreen Assay

    Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A to C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD; n = 3 isolations per sample.

    Journal: Science Advances

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    doi: 10.1126/sciadv.1600502

    Figure Lengend Snippet: Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A to C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD; n = 3 isolations per sample.

    Article Snippet: The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific.

    Techniques: Nucleic Acid Concentration, Concentration Assay, Isolation

    Antiproliferative activity of barbatolic acid on HUVEC, HCC1428 and T-47D cell lines. a AlamarBlue™ cell viability assay, b lactate dehydrogenase (LDH) cellular membrane degradation assay and c PicoGreen™ dsDNA quantitation assay. Data

    Journal: Cytotechnology

    Article Title: Anti-breast cancer and anti-angiogenic potential of a lichen-derived small-molecule: barbatolic acid

    doi: 10.1007/s10616-018-0249-x

    Figure Lengend Snippet: Antiproliferative activity of barbatolic acid on HUVEC, HCC1428 and T-47D cell lines. a AlamarBlue™ cell viability assay, b lactate dehydrogenase (LDH) cellular membrane degradation assay and c PicoGreen™ dsDNA quantitation assay. Data

    Article Snippet: AlamarBlue™ cell viability kit and Quant-iT™ PicoGreen™ dsDNA assay kit were obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA).

    Techniques: Activity Assay, Viability Assay, Degradation Assay, Quantitation Assay