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    Thermo Fisher qrt pcr single stranded cdna
    Effects of HWB treatment on the expression of flavonoid biosynthesis-related genes and the occurrence of red lenticel discoloration on mango fruit cv. Shelly. (A) <t>qRT-PCR</t> profile of differentially expressed genes Ugft3, PAL, CFIL and CHSï , which are related to the flavonoid biosynthesis process, naringenin-chalcone synthase activity, and the phenylpropanoid biosynthesis pathway. (B) level of lenticel discoloration of HWB-treated and control fruits, and (C) lenticel discoloration symptoms on mango fruits, cv. Shelly following HWB treatment. qRT-PCR values were normalized to the values obtained in samples from untreated mango fruits at 0 h. Expression data are means of two replicates. Lenticel discoloration was evaluated following 2 weeks of storage at 12°C [ 9 ]. Average values followed by different letters differ significantly at P
    Qrt Pcr Single Stranded Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr single stranded cdna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qrt pcr single stranded cdna - by Bioz Stars, 2020-08
    86/100 stars

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    1) Product Images from "De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment"

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-15-957

    Effects of HWB treatment on the expression of flavonoid biosynthesis-related genes and the occurrence of red lenticel discoloration on mango fruit cv. Shelly. (A) qRT-PCR profile of differentially expressed genes Ugft3, PAL, CFIL and CHSï , which are related to the flavonoid biosynthesis process, naringenin-chalcone synthase activity, and the phenylpropanoid biosynthesis pathway. (B) level of lenticel discoloration of HWB-treated and control fruits, and (C) lenticel discoloration symptoms on mango fruits, cv. Shelly following HWB treatment. qRT-PCR values were normalized to the values obtained in samples from untreated mango fruits at 0 h. Expression data are means of two replicates. Lenticel discoloration was evaluated following 2 weeks of storage at 12°C [ 9 ]. Average values followed by different letters differ significantly at P
    Figure Legend Snippet: Effects of HWB treatment on the expression of flavonoid biosynthesis-related genes and the occurrence of red lenticel discoloration on mango fruit cv. Shelly. (A) qRT-PCR profile of differentially expressed genes Ugft3, PAL, CFIL and CHSï , which are related to the flavonoid biosynthesis process, naringenin-chalcone synthase activity, and the phenylpropanoid biosynthesis pathway. (B) level of lenticel discoloration of HWB-treated and control fruits, and (C) lenticel discoloration symptoms on mango fruits, cv. Shelly following HWB treatment. qRT-PCR values were normalized to the values obtained in samples from untreated mango fruits at 0 h. Expression data are means of two replicates. Lenticel discoloration was evaluated following 2 weeks of storage at 12°C [ 9 ]. Average values followed by different letters differ significantly at P

    Techniques Used: Expressing, Quantitative RT-PCR, Activity Assay

    Shelly. Effect of HWB on differential expression of chlorophyll and anthocyanin accumulation-related genes and color development in mango cv. Shelly. (A, B) qRT-PCR gene-expression profiles of genes related to (A) chlorophyll accumulation ( Thl1ch , LHCIIb, Oxepch and PIRC ) and (B) anthocyanin synthesis ( 85A2 and Anthocyanin5 ). The expression profile comprises data taken from samples of mango tissues sampled from cv. Shelly at four different time points after HWB treatment. (C) Changes in color index after 16 days of storage at 12°C followed by 8 days at 20°C. Vertical bars indicate SD of five replicates. qRT-PCR values were normalized to the values obtained with untreated mango fruit samples at 0 h. Expression data are the means of two replicates.
    Figure Legend Snippet: Shelly. Effect of HWB on differential expression of chlorophyll and anthocyanin accumulation-related genes and color development in mango cv. Shelly. (A, B) qRT-PCR gene-expression profiles of genes related to (A) chlorophyll accumulation ( Thl1ch , LHCIIb, Oxepch and PIRC ) and (B) anthocyanin synthesis ( 85A2 and Anthocyanin5 ). The expression profile comprises data taken from samples of mango tissues sampled from cv. Shelly at four different time points after HWB treatment. (C) Changes in color index after 16 days of storage at 12°C followed by 8 days at 20°C. Vertical bars indicate SD of five replicates. qRT-PCR values were normalized to the values obtained with untreated mango fruit samples at 0 h. Expression data are the means of two replicates.

    Techniques Used: Expressing, Quantitative RT-PCR

    Validation of RNA-seq results by means of qRT-PCR. Ten differentially expressed genes (two from each of clusters 1 to 5) were examined by RNA-seq and qRT-PCR at four different time points after HWB treatment: A , B (cluster 1); C , D (cluster 2); E , F (cluster 3); G , H (cluster 4); and I , J (cluster 5). Values were normalized to the values obtained with untreated mango fruit samples at 0 h and the proportional fold-change (FC) was calculated. Expression data are means of two replicates.
    Figure Legend Snippet: Validation of RNA-seq results by means of qRT-PCR. Ten differentially expressed genes (two from each of clusters 1 to 5) were examined by RNA-seq and qRT-PCR at four different time points after HWB treatment: A , B (cluster 1); C , D (cluster 2); E , F (cluster 3); G , H (cluster 4); and I , J (cluster 5). Values were normalized to the values obtained with untreated mango fruit samples at 0 h and the proportional fold-change (FC) was calculated. Expression data are means of two replicates.

    Techniques Used: RNA Sequencing Assay, Quantitative RT-PCR, Expressing

    Differential expressions of genes modulating the mechanism of resistance to A. alternata in naturally infected mango fruits. (A) Effect of HWB on alternaria black spot (ABS) symptom development on mango cvs. Palmer, Kent, Tommy Atkins, Keitt, Lily and Shelly. (B) ABS symptom development on naturally infected fruits cv. Keitt following HWB treatment. (C) qRT-PCR differential expression profiling of genes Syn121 , glutardoxin , IT1K2 and AOS of cv. Shelly. Fruit peel tissues were sampled at four different time points after HWB treatment. RNA was extracted and served as a template for cDNA followed by qRT-PCR analysis of the genes of interest. Proportional increases in relative expression values were normalized against the samples of untreated mango fruits at 0 h. Expression data are means of two replicates. ABS-covered area was evaluated after 4 weeks of storage at 12°C. Average values followed by different letters differ significantly at P
    Figure Legend Snippet: Differential expressions of genes modulating the mechanism of resistance to A. alternata in naturally infected mango fruits. (A) Effect of HWB on alternaria black spot (ABS) symptom development on mango cvs. Palmer, Kent, Tommy Atkins, Keitt, Lily and Shelly. (B) ABS symptom development on naturally infected fruits cv. Keitt following HWB treatment. (C) qRT-PCR differential expression profiling of genes Syn121 , glutardoxin , IT1K2 and AOS of cv. Shelly. Fruit peel tissues were sampled at four different time points after HWB treatment. RNA was extracted and served as a template for cDNA followed by qRT-PCR analysis of the genes of interest. Proportional increases in relative expression values were normalized against the samples of untreated mango fruits at 0 h. Expression data are means of two replicates. ABS-covered area was evaluated after 4 weeks of storage at 12°C. Average values followed by different letters differ significantly at P

    Techniques Used: Infection, Quantitative RT-PCR, Expressing

    Related Articles

    Quantitative RT-PCR:

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment
    Article Snippet: .. Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The synthesized cDNA was used as a template for qRT-PCR analysis, to estimate the expression level of the selected genes.

    Synthesized:

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment
    Article Snippet: .. Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The synthesized cDNA was used as a template for qRT-PCR analysis, to estimate the expression level of the selected genes.

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    Thermo Fisher qrt pcr single stranded cdna
    Effects of HWB treatment on the expression of flavonoid biosynthesis-related genes and the occurrence of red lenticel discoloration on mango fruit cv. Shelly. (A) <t>qRT-PCR</t> profile of differentially expressed genes Ugft3, PAL, CFIL and CHSï , which are related to the flavonoid biosynthesis process, naringenin-chalcone synthase activity, and the phenylpropanoid biosynthesis pathway. (B) level of lenticel discoloration of HWB-treated and control fruits, and (C) lenticel discoloration symptoms on mango fruits, cv. Shelly following HWB treatment. qRT-PCR values were normalized to the values obtained in samples from untreated mango fruits at 0 h. Expression data are means of two replicates. Lenticel discoloration was evaluated following 2 weeks of storage at 12°C [ 9 ]. Average values followed by different letters differ significantly at P
    Qrt Pcr Single Stranded Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr single stranded cdna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qrt pcr single stranded cdna - by Bioz Stars, 2020-08
    86/100 stars
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    Effects of HWB treatment on the expression of flavonoid biosynthesis-related genes and the occurrence of red lenticel discoloration on mango fruit cv. Shelly. (A) qRT-PCR profile of differentially expressed genes Ugft3, PAL, CFIL and CHSï , which are related to the flavonoid biosynthesis process, naringenin-chalcone synthase activity, and the phenylpropanoid biosynthesis pathway. (B) level of lenticel discoloration of HWB-treated and control fruits, and (C) lenticel discoloration symptoms on mango fruits, cv. Shelly following HWB treatment. qRT-PCR values were normalized to the values obtained in samples from untreated mango fruits at 0 h. Expression data are means of two replicates. Lenticel discoloration was evaluated following 2 weeks of storage at 12°C [ 9 ]. Average values followed by different letters differ significantly at P

    Journal: BMC Genomics

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment

    doi: 10.1186/1471-2164-15-957

    Figure Lengend Snippet: Effects of HWB treatment on the expression of flavonoid biosynthesis-related genes and the occurrence of red lenticel discoloration on mango fruit cv. Shelly. (A) qRT-PCR profile of differentially expressed genes Ugft3, PAL, CFIL and CHSï , which are related to the flavonoid biosynthesis process, naringenin-chalcone synthase activity, and the phenylpropanoid biosynthesis pathway. (B) level of lenticel discoloration of HWB-treated and control fruits, and (C) lenticel discoloration symptoms on mango fruits, cv. Shelly following HWB treatment. qRT-PCR values were normalized to the values obtained in samples from untreated mango fruits at 0 h. Expression data are means of two replicates. Lenticel discoloration was evaluated following 2 weeks of storage at 12°C [ 9 ]. Average values followed by different letters differ significantly at P

    Article Snippet: Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Activity Assay

    Shelly. Effect of HWB on differential expression of chlorophyll and anthocyanin accumulation-related genes and color development in mango cv. Shelly. (A, B) qRT-PCR gene-expression profiles of genes related to (A) chlorophyll accumulation ( Thl1ch , LHCIIb, Oxepch and PIRC ) and (B) anthocyanin synthesis ( 85A2 and Anthocyanin5 ). The expression profile comprises data taken from samples of mango tissues sampled from cv. Shelly at four different time points after HWB treatment. (C) Changes in color index after 16 days of storage at 12°C followed by 8 days at 20°C. Vertical bars indicate SD of five replicates. qRT-PCR values were normalized to the values obtained with untreated mango fruit samples at 0 h. Expression data are the means of two replicates.

    Journal: BMC Genomics

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment

    doi: 10.1186/1471-2164-15-957

    Figure Lengend Snippet: Shelly. Effect of HWB on differential expression of chlorophyll and anthocyanin accumulation-related genes and color development in mango cv. Shelly. (A, B) qRT-PCR gene-expression profiles of genes related to (A) chlorophyll accumulation ( Thl1ch , LHCIIb, Oxepch and PIRC ) and (B) anthocyanin synthesis ( 85A2 and Anthocyanin5 ). The expression profile comprises data taken from samples of mango tissues sampled from cv. Shelly at four different time points after HWB treatment. (C) Changes in color index after 16 days of storage at 12°C followed by 8 days at 20°C. Vertical bars indicate SD of five replicates. qRT-PCR values were normalized to the values obtained with untreated mango fruit samples at 0 h. Expression data are the means of two replicates.

    Article Snippet: Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Quantitative RT-PCR

    Validation of RNA-seq results by means of qRT-PCR. Ten differentially expressed genes (two from each of clusters 1 to 5) were examined by RNA-seq and qRT-PCR at four different time points after HWB treatment: A , B (cluster 1); C , D (cluster 2); E , F (cluster 3); G , H (cluster 4); and I , J (cluster 5). Values were normalized to the values obtained with untreated mango fruit samples at 0 h and the proportional fold-change (FC) was calculated. Expression data are means of two replicates.

    Journal: BMC Genomics

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment

    doi: 10.1186/1471-2164-15-957

    Figure Lengend Snippet: Validation of RNA-seq results by means of qRT-PCR. Ten differentially expressed genes (two from each of clusters 1 to 5) were examined by RNA-seq and qRT-PCR at four different time points after HWB treatment: A , B (cluster 1); C , D (cluster 2); E , F (cluster 3); G , H (cluster 4); and I , J (cluster 5). Values were normalized to the values obtained with untreated mango fruit samples at 0 h and the proportional fold-change (FC) was calculated. Expression data are means of two replicates.

    Article Snippet: Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Expressing

    Differential expressions of genes modulating the mechanism of resistance to A. alternata in naturally infected mango fruits. (A) Effect of HWB on alternaria black spot (ABS) symptom development on mango cvs. Palmer, Kent, Tommy Atkins, Keitt, Lily and Shelly. (B) ABS symptom development on naturally infected fruits cv. Keitt following HWB treatment. (C) qRT-PCR differential expression profiling of genes Syn121 , glutardoxin , IT1K2 and AOS of cv. Shelly. Fruit peel tissues were sampled at four different time points after HWB treatment. RNA was extracted and served as a template for cDNA followed by qRT-PCR analysis of the genes of interest. Proportional increases in relative expression values were normalized against the samples of untreated mango fruits at 0 h. Expression data are means of two replicates. ABS-covered area was evaluated after 4 weeks of storage at 12°C. Average values followed by different letters differ significantly at P

    Journal: BMC Genomics

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment

    doi: 10.1186/1471-2164-15-957

    Figure Lengend Snippet: Differential expressions of genes modulating the mechanism of resistance to A. alternata in naturally infected mango fruits. (A) Effect of HWB on alternaria black spot (ABS) symptom development on mango cvs. Palmer, Kent, Tommy Atkins, Keitt, Lily and Shelly. (B) ABS symptom development on naturally infected fruits cv. Keitt following HWB treatment. (C) qRT-PCR differential expression profiling of genes Syn121 , glutardoxin , IT1K2 and AOS of cv. Shelly. Fruit peel tissues were sampled at four different time points after HWB treatment. RNA was extracted and served as a template for cDNA followed by qRT-PCR analysis of the genes of interest. Proportional increases in relative expression values were normalized against the samples of untreated mango fruits at 0 h. Expression data are means of two replicates. ABS-covered area was evaluated after 4 weeks of storage at 12°C. Average values followed by different letters differ significantly at P

    Article Snippet: Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Infection, Quantitative RT-PCR, Expressing