Structured Review

Roche qrt pcr lightcycler 96 instrument
Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for <t>qRT-PCR</t> assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.
Qrt Pcr Lightcycler 96 Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qrt pcr lightcycler 96 instrument/product/Roche
Average 86 stars, based on 3 article reviews
Price from $9.99 to $1999.99
qrt pcr lightcycler 96 instrument - by Bioz Stars, 2020-02
86/100 stars

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1) Product Images from "Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering"

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

Journal: Scientific Reports

doi: 10.1038/s41598-018-33242-z

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) untreated rat rectus abdominis muscle tissue, ( B ) rat rectus abdominis muscle tissue treated with osteogenic medium, and ( C ) both normal and treated rat muscle tissue.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) untreated rat rectus abdominis muscle tissue, ( B ) rat rectus abdominis muscle tissue treated with osteogenic medium, and ( C ) both normal and treated rat muscle tissue.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

2) Product Images from "Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering"

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

Journal: Scientific Reports

doi: 10.1038/s41598-018-33242-z

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

3) Product Images from "Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering"

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

Journal: Scientific Reports

doi: 10.1038/s41598-018-33242-z

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) untreated rat rectus abdominis muscle tissue, ( B ) rat rectus abdominis muscle tissue treated with osteogenic medium, and ( C ) both normal and treated rat muscle tissue.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) untreated rat rectus abdominis muscle tissue, ( B ) rat rectus abdominis muscle tissue treated with osteogenic medium, and ( C ) both normal and treated rat muscle tissue.

Techniques Used: Expressing, Quantitative RT-PCR

Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.
Figure Legend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.

Techniques Used: Expressing, Quantitative RT-PCR

Related Articles

Polymerase Chain Reaction:

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: .. The PCR reactions were performed using a qRT-PCR LightCycler® 96 Instrument (Roche, Basel, Swiss), where the total volume per reaction was 10 μl, containing 10 μM of each reference primer (Table ), the corresponding diluted cDNA and 2x FastStart Essential DNA Green Master (Roche). .. Standardisation runs were performed using a LightCycler® 96 thermocycler (Roche, Basel, Swiss), with thermocycling parameters possessing a pre-incubation of 3 min at 95 °C, followed by a three step amplification programme of 40 cycles consisting of a denaturation, annealing and extension step set at 95 °C for 10 s, 60 °C for 15 s and 72 °C for 30 s, respectively.

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: .. PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche). .. Thermocycling parameters had a pre-incubation step of 3 min at 95 °C, followed by a three step amplification programme of 40 cycles consisting of a denaturation, annealing and extension step set at 95 °C for 10 s, 60 °C for 15 s and 72 °C for 30 s, respectively.

Generated:

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche). .. Generated data was then inputted into GeNorm ( http://medgen.ugent.be/wjvdesomp/genorm/ ) using the relative quantities based on comparative Cq method .

Amplification:

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: The PCR reactions were performed using a qRT-PCR LightCycler® 96 Instrument (Roche, Basel, Swiss), where the total volume per reaction was 10 μl, containing 10 μM of each reference primer (Table ), the corresponding diluted cDNA and 2x FastStart Essential DNA Green Master (Roche). .. Standardisation runs were performed using a LightCycler® 96 thermocycler (Roche, Basel, Swiss), with thermocycling parameters possessing a pre-incubation of 3 min at 95 °C, followed by a three step amplification programme of 40 cycles consisting of a denaturation, annealing and extension step set at 95 °C for 10 s, 60 °C for 15 s and 72 °C for 30 s, respectively.

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche). .. Thermocycling parameters had a pre-incubation step of 3 min at 95 °C, followed by a three step amplification programme of 40 cycles consisting of a denaturation, annealing and extension step set at 95 °C for 10 s, 60 °C for 15 s and 72 °C for 30 s, respectively.

Quantitative RT-PCR:

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: .. The PCR reactions were performed using a qRT-PCR LightCycler® 96 Instrument (Roche, Basel, Swiss), where the total volume per reaction was 10 μl, containing 10 μM of each reference primer (Table ), the corresponding diluted cDNA and 2x FastStart Essential DNA Green Master (Roche). .. Standardisation runs were performed using a LightCycler® 96 thermocycler (Roche, Basel, Swiss), with thermocycling parameters possessing a pre-incubation of 3 min at 95 °C, followed by a three step amplification programme of 40 cycles consisting of a denaturation, annealing and extension step set at 95 °C for 10 s, 60 °C for 15 s and 72 °C for 30 s, respectively.

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: .. PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche). .. Thermocycling parameters had a pre-incubation step of 3 min at 95 °C, followed by a three step amplification programme of 40 cycles consisting of a denaturation, annealing and extension step set at 95 °C for 10 s, 60 °C for 15 s and 72 °C for 30 s, respectively.

Expressing:

Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering
Article Snippet: Paragraph title: Reference primer expression stability and quantity ... PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche).

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    Roche qrt pcr lightcycler 96 instrument
    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for <t>qRT-PCR</t> assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.
    Qrt Pcr Lightcycler 96 Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr lightcycler 96 instrument/product/Roche
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    qrt pcr lightcycler 96 instrument - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    80
    Roche lightcycler 96 instrument qrt pcr system
    Quantitative real-time <t>PCR</t> validation of differentially expressed miRNAs and their corresponding target mRNAs. The red dots indicate three biological repetition of gene in <t>qRT-PCR</t> result. The blue dots indicate three biological repetition of miRNA in qRT-PCR result. (A) The qRT-PCR validation of miR-30a-3p and its target gene FOXO3 ; (B) The qRT-PCR validation of miR-30a-3p and its target gene DYNLL2; (C) The qRT-PCR validation of miR-148a-3p and its target gene DYNLL2.
    Lightcycler 96 Instrument Qrt Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 96 instrument qrt pcr system/product/Roche
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lightcycler 96 instrument qrt pcr system - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    Image Search Results


    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.

    Article Snippet: PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.

    Article Snippet: PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.

    Article Snippet: PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) untreated rat rectus abdominis muscle tissue, ( B ) rat rectus abdominis muscle tissue treated with osteogenic medium, and ( C ) both normal and treated rat muscle tissue.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) untreated rat rectus abdominis muscle tissue, ( B ) rat rectus abdominis muscle tissue treated with osteogenic medium, and ( C ) both normal and treated rat muscle tissue.

    Article Snippet: PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.

    Article Snippet: PCR reactions were carried out in 96-well plates in duplicate utilising a qRT-PCR LightCycler® 96 Instrument (Roche) with a total reaction volume of 10 μl, that contained 10 μM of each reference primer (Table ), 10 ng of cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm. ( A ) GeNorm results between Control cell groups with normal hChondrocytes, hBMSCs and hADSCs. ( B ) GeNorm results between treated cell groups, i.e. hChondrocytes undergoing apoptosis, chondrogenic differentiated hBMSCs and hADSCs. ( C ) GeNorm results between all untreated and treated cell lines and types.

    Article Snippet: The PCR reactions were performed using a qRT-PCR LightCycler® 96 Instrument (Roche, Basel, Swiss), where the total volume per reaction was 10 μl, containing 10 μM of each reference primer (Table ), the corresponding diluted cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hADSCs and ( B ) chondrogenic differentiated hADSCs separately or ( C ) both untreated and treated hADSCs cell lines combined.

    Article Snippet: The PCR reactions were performed using a qRT-PCR LightCycler® 96 Instrument (Roche, Basel, Swiss), where the total volume per reaction was 10 μl, containing 10 μM of each reference primer (Table ), the corresponding diluted cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal human Chondrocytes (hChondrocytes) and ( B ) hChondrocytes undergoing apoptosis or ( C ) both normal and apoptotic chondrocyte cell lines combined.

    Article Snippet: The PCR reactions were performed using a qRT-PCR LightCycler® 96 Instrument (Roche, Basel, Swiss), where the total volume per reaction was 10 μl, containing 10 μM of each reference primer (Table ), the corresponding diluted cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.

    Journal: Scientific Reports

    Article Title: Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering

    doi: 10.1038/s41598-018-33242-z

    Figure Lengend Snippet: Average expression stability ( A1 , B1 , C1 ) and optimal number of reference genes for normalization ( A2 , B2 , C2 ) for qRT-PCR assays, utilizing the GeNorm algorithm, for ( A ) normal hBMSCs and ( B ) chondrogenic differentiated hBMSCs separately or ( C ) both cell lines combined.

    Article Snippet: The PCR reactions were performed using a qRT-PCR LightCycler® 96 Instrument (Roche, Basel, Swiss), where the total volume per reaction was 10 μl, containing 10 μM of each reference primer (Table ), the corresponding diluted cDNA and 2x FastStart Essential DNA Green Master (Roche).

    Techniques: Expressing, Quantitative RT-PCR

    Quantitative real-time PCR validation of differentially expressed miRNAs and their corresponding target mRNAs. The red dots indicate three biological repetition of gene in qRT-PCR result. The blue dots indicate three biological repetition of miRNA in qRT-PCR result. (A) The qRT-PCR validation of miR-30a-3p and its target gene FOXO3 ; (B) The qRT-PCR validation of miR-30a-3p and its target gene DYNLL2; (C) The qRT-PCR validation of miR-148a-3p and its target gene DYNLL2.

    Journal: Frontiers in Genetics

    Article Title: Analyses of MicroRNA and mRNA Expression Profiles Reveal the Crucial Interaction Networks and Pathways for Regulation of Chicken Breast Muscle Development

    doi: 10.3389/fgene.2019.00197

    Figure Lengend Snippet: Quantitative real-time PCR validation of differentially expressed miRNAs and their corresponding target mRNAs. The red dots indicate three biological repetition of gene in qRT-PCR result. The blue dots indicate three biological repetition of miRNA in qRT-PCR result. (A) The qRT-PCR validation of miR-30a-3p and its target gene FOXO3 ; (B) The qRT-PCR validation of miR-30a-3p and its target gene DYNLL2; (C) The qRT-PCR validation of miR-148a-3p and its target gene DYNLL2.

    Article Snippet: Quantitative Real-Time PCR (qRT-PCR) Analysis For the qRT-PCR analysis of genes, reverse transcription was performed using a PrimerScriptTM RT reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions. qRT-PCR with iTaqTM Universal SYBR® Green Supermix Kit (Bio-Rad Laboratories Inc., Waltham, MA, United States) was performed with a LightCycler® 96 instrument qRT-PCR system (Roche, Basel, Switzerland) as follows: 95°C for 3 min; 40 cycles of 95°C for 10 s, annealing at 60°C for 30 s, and 72°C for 30 s, and 72°C for 1 min.

    Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Quantitative real-time PCR (qRT-PCR) validation of differentially expressed mRNAs. The red dots indicate the biological repetition in qRT-PCR result. The blue dots indicate the biological repetition from RNA-seq.

    Journal: Frontiers in Genetics

    Article Title: Analyses of MicroRNA and mRNA Expression Profiles Reveal the Crucial Interaction Networks and Pathways for Regulation of Chicken Breast Muscle Development

    doi: 10.3389/fgene.2019.00197

    Figure Lengend Snippet: Quantitative real-time PCR (qRT-PCR) validation of differentially expressed mRNAs. The red dots indicate the biological repetition in qRT-PCR result. The blue dots indicate the biological repetition from RNA-seq.

    Article Snippet: Quantitative Real-Time PCR (qRT-PCR) Analysis For the qRT-PCR analysis of genes, reverse transcription was performed using a PrimerScriptTM RT reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions. qRT-PCR with iTaqTM Universal SYBR® Green Supermix Kit (Bio-Rad Laboratories Inc., Waltham, MA, United States) was performed with a LightCycler® 96 instrument qRT-PCR system (Roche, Basel, Switzerland) as follows: 95°C for 3 min; 40 cycles of 95°C for 10 s, annealing at 60°C for 30 s, and 72°C for 30 s, and 72°C for 1 min.

    Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, RNA Sequencing Assay