qiaspin pcr purification kit  (Qiagen)

 
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    Name:
    QIAquick PCR Purification Kit
    Description:
    For purification of up to 10 μg PCR products 100 bp to 10 kb Kit contents Qiagen QIAquick PCR Purification Kit 50 rxns 30L Elution Volume 10g Binding Capacity Tube Format Manual Processing Silica Technology 100 bp to 10 kb Fragment 40mers Fragments Removed Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and in vitro Transcription For Purification of up to 10μg PCR Products Includes 50 QIAquick Spin Columns Buffers 2mL Collection Tubes Benefits Up to 95 recovery of ready to use DNA Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    Catalog Number:
    28104
    Price:
    117
    Category:
    QIAquick PCR Purification Kit
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    Structured Review

    Qiagen qiaspin pcr purification kit
    QIAquick PCR Purification Kit
    For purification of up to 10 μg PCR products 100 bp to 10 kb Kit contents Qiagen QIAquick PCR Purification Kit 50 rxns 30L Elution Volume 10g Binding Capacity Tube Format Manual Processing Silica Technology 100 bp to 10 kb Fragment 40mers Fragments Removed Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and in vitro Transcription For Purification of up to 10μg PCR Products Includes 50 QIAquick Spin Columns Buffers 2mL Collection Tubes Benefits Up to 95 recovery of ready to use DNA Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/qiaspin pcr purification kit/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiaspin pcr purification kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Transfection:

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Purification:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick). ..

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: .. In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104). ..

    Article Title: Usp9x regulates Ets-1 ubiquitination and stability to control NRAS expression and tumorigenicity in melanoma
    Article Snippet: .. ChIP DNA was purified using a Quick PCR Purification Kit (Qiagen). ..

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Article Title: Use of a restriction enzyme-digested PCR product as substrate for helicase assays
    Article Snippet: .. Following PCR, the product was purified using QIAquick PCR purification kit (Qiagen) and digested with restriction enzymes that generate either a 3′ or a 5′ 4-base ssDNA overhang. ..

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: .. Kit extractions We tested three different silica-column kits: Zymo ZR Viral DNA/RNA Kit (outdated protocol, D7021), Zymo Quick-DNA/RNA Kit (updated protocol, D7021), and the QIAquick PCR Purification Kit (28104, Qiagen). .. For all silica-column kits, fresh collection tubes were used after each spin and centrifugation speeds were set to 16,000 × g. Centrifugation was performed on either an Eppendorf 5415D centrifuge (Eppendorf, Hauppauge, NY, USA) or a Thermo Fisher Scientific AccuSpin Micro 17 R centrifuge (13–100–676).

    Article Title: Normal histone modifications on the inactive X chromosome in ICF and Rett syndrome cells: implications for methyl-CpG binding proteins
    Article Snippet: .. After elution of immune complexes, they were heated at 65°C for 4 h to reverse crosslinks, and the DNA was recovered with a "QIAquick" PCR purification kit from Qiagen Inc. (Valencia, CA). .. Primary amplification of AR DNA was across the polymorphic 5' CAG repeat region as previously described [ ], using 27–35 cycles of PCR amplification with 10% of the immunoprecipitated material or 50 ng of input DNA in a 50 μl reaction volume.

    Electrophoresis:

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Immunoprecipitation:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick). ..

    Incubation:

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: .. In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104). ..

    Polymerase Chain Reaction:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick). ..

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: .. In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104). ..

    Article Title: Usp9x regulates Ets-1 ubiquitination and stability to control NRAS expression and tumorigenicity in melanoma
    Article Snippet: .. ChIP DNA was purified using a Quick PCR Purification Kit (Qiagen). ..

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Article Title: Use of a restriction enzyme-digested PCR product as substrate for helicase assays
    Article Snippet: .. Following PCR, the product was purified using QIAquick PCR purification kit (Qiagen) and digested with restriction enzymes that generate either a 3′ or a 5′ 4-base ssDNA overhang. ..

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: .. Kit extractions We tested three different silica-column kits: Zymo ZR Viral DNA/RNA Kit (outdated protocol, D7021), Zymo Quick-DNA/RNA Kit (updated protocol, D7021), and the QIAquick PCR Purification Kit (28104, Qiagen). .. For all silica-column kits, fresh collection tubes were used after each spin and centrifugation speeds were set to 16,000 × g. Centrifugation was performed on either an Eppendorf 5415D centrifuge (Eppendorf, Hauppauge, NY, USA) or a Thermo Fisher Scientific AccuSpin Micro 17 R centrifuge (13–100–676).

    Article Title: Normal histone modifications on the inactive X chromosome in ICF and Rett syndrome cells: implications for methyl-CpG binding proteins
    Article Snippet: .. After elution of immune complexes, they were heated at 65°C for 4 h to reverse crosslinks, and the DNA was recovered with a "QIAquick" PCR purification kit from Qiagen Inc. (Valencia, CA). .. Primary amplification of AR DNA was across the polymorphic 5' CAG repeat region as previously described [ ], using 27–35 cycles of PCR amplification with 10% of the immunoprecipitated material or 50 ng of input DNA in a 50 μl reaction volume.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Chromatin Immunoprecipitation:

    Article Title: Usp9x regulates Ets-1 ubiquitination and stability to control NRAS expression and tumorigenicity in melanoma
    Article Snippet: .. ChIP DNA was purified using a Quick PCR Purification Kit (Qiagen). ..

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  • News
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  • 99
    Qiagen qiaspin pcr purification kit
    Qiaspin Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaspin pcr purification kit/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiaspin pcr purification kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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