qiashredder spin column  (Qiagen)

 
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    Name:
    QIAshredder
    Description:
    For simple and rapid homogenization of cell and tissue lysates Kit contents Qiagen QIAshredder 50 Disposable Cell lysate Homogenizers for use in Nucleic acid preps Caps For Simple and Rapid Homogenization of Cell and Tissue Lysates Replaces Syringe and needle Homogenization Reduces Loss of Sample Material Eliminates Cross contamination Between Samples Filters out Insoluble Debris and Reduces Viscosity Spin column Format QIAshredder Homogenizer Placed in a Collection Tube and Centrifuged Benefits Replaces syringe and needle homogenization Reduces loss of sample material Eliminates cross contamination between samples Filters out insoluble debris and reduces viscosity
    Catalog Number:
    79654
    Price:
    88
    Category:
    QIAshredder
    Buy from Supplier


    Structured Review

    Qiagen qiashredder spin column
    QIAshredder
    For simple and rapid homogenization of cell and tissue lysates Kit contents Qiagen QIAshredder 50 Disposable Cell lysate Homogenizers for use in Nucleic acid preps Caps For Simple and Rapid Homogenization of Cell and Tissue Lysates Replaces Syringe and needle Homogenization Reduces Loss of Sample Material Eliminates Cross contamination Between Samples Filters out Insoluble Debris and Reduces Viscosity Spin column Format QIAshredder Homogenizer Placed in a Collection Tube and Centrifuged Benefits Replaces syringe and needle homogenization Reduces loss of sample material Eliminates cross contamination between samples Filters out insoluble debris and reduces viscosity
    https://www.bioz.com/result/qiashredder spin column/product/Qiagen
    Average 99 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    qiashredder spin column - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract"

    Article Title: Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0057011

    View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.
    Figure Legend Snippet: View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.

    Techniques Used: Derivative Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Infection

    2) Product Images from "Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract"

    Article Title: Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0057011

    View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.
    Figure Legend Snippet: View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.

    Techniques Used: Derivative Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Infection

    Related Articles

    Centrifugation:

    Article Title: Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract
    Article Snippet: .. Next, they were shredded using a QIAshredder spin column (QIAGEN) with centrifugation for 2 min at 5000×g . .. After centrifugation, 400 µL flow- through solution was collected and designated QIAshredder-flow-through .

    Homogenization:

    Article Title: New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures
    Article Snippet: .. Method A: lysis of the mononuclear cells, followed by lysate homogenization using a biopolymer shredding system in a microcentrifuge spin-column format (QIAshredder, Qiagen, Hilden, Germany), followed by total RNA purification using selective binding columns (RNeasy Mini Kit, Qiagen). .. The cell lysate homogenization phase reduces viscosity caused by high-molecular-weight cellular components and cell debris.

    Spectrophotometry:

    Article Title: Phase-I trial of survivin inhibition with EZN-3042 in dogs with spontaneous lymphoma
    Article Snippet: .. Briefly, RNA was extracted using the Qiashredder and RNeasy Kit (Qiagen, Chatsworth, CA) and quality assessed using a NanoDrop spectrophotometer (ThermoFisher, Waltham, MA). .. RNA was reverse-transcribed using the Omniscript® RT Kit (Qiagen).

    Purification:

    Article Title: New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures
    Article Snippet: .. Method A: lysis of the mononuclear cells, followed by lysate homogenization using a biopolymer shredding system in a microcentrifuge spin-column format (QIAshredder, Qiagen, Hilden, Germany), followed by total RNA purification using selective binding columns (RNeasy Mini Kit, Qiagen). .. The cell lysate homogenization phase reduces viscosity caused by high-molecular-weight cellular components and cell debris.

    Modification:

    Article Title: Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract
    Article Snippet: .. However, when the preparation using QIAshredder spin column will be modified, the sensitivity of detection may be improved. .. The preparation of templates for direct PCR using mini homogenizer tubes such as Biomasher III is very easy.

    Lysis:

    Article Title: New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures
    Article Snippet: .. Method A: lysis of the mononuclear cells, followed by lysate homogenization using a biopolymer shredding system in a microcentrifuge spin-column format (QIAshredder, Qiagen, Hilden, Germany), followed by total RNA purification using selective binding columns (RNeasy Mini Kit, Qiagen). .. The cell lysate homogenization phase reduces viscosity caused by high-molecular-weight cellular components and cell debris.

    Binding Assay:

    Article Title: New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures
    Article Snippet: .. Method A: lysis of the mononuclear cells, followed by lysate homogenization using a biopolymer shredding system in a microcentrifuge spin-column format (QIAshredder, Qiagen, Hilden, Germany), followed by total RNA purification using selective binding columns (RNeasy Mini Kit, Qiagen). .. The cell lysate homogenization phase reduces viscosity caused by high-molecular-weight cellular components and cell debris.

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    Qiagen qiashredder spin column
    View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and <t>QIAshredder-pellet</t> was performed to derive each Ct value. The numbers in each graph are Ct values.
    Qiashredder Spin Column, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiashredder spin column/product/Qiagen
    Average 99 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    qiashredder spin column - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.

    Journal: PLoS ONE

    Article Title: Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract

    doi: 10.1371/journal.pone.0057011

    Figure Lengend Snippet: View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.

    Article Snippet: However, when the preparation using QIAshredder spin column will be modified, the sensitivity of detection may be improved.

    Techniques: Derivative Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Infection

    Study concept . (A) Total RNA of each of the first 24 samples had been extracted following three different total RNA purification methods A, B, and C. Method A: lysis of the mononuclear cells, followed by lysate homogenization (to reduce viscosity caused by high-molecular-weight cellular components and cell debris) using a biopolymer shredding system in a microcentrifuge spin-column format (QIAshredder, Qiagen) followed by total RNA purification (RNeasy Mini Kit, Qiagen). Method B: TRIzol RNA isolation (Invitrogen). Method C: TRIzol RNA isolation (Invitrogen) followed by an RNeasy purification step (RNeasy Mini Kit, Qiagen). The RNA purification step combines the selective binding properties of a silica-based membrane with the speed of microspin technology. It allows only RNA longer than 200 bases to bind to the silica membrane, providing an enriching for mRNA since nucleotides shorter than 200 nucleotides are selectively excluded. (B) For each of three additional samples, nine aliquots of mononuclear cells had been collected. Total RNA has been processed for each aliquot following one of the three methods and for each method three independent technical replicates were performed (A,A,A, B,B,B, C,C,C).

    Journal: BMC Genomics

    Article Title: New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures

    doi: 10.1186/1471-2164-8-188

    Figure Lengend Snippet: Study concept . (A) Total RNA of each of the first 24 samples had been extracted following three different total RNA purification methods A, B, and C. Method A: lysis of the mononuclear cells, followed by lysate homogenization (to reduce viscosity caused by high-molecular-weight cellular components and cell debris) using a biopolymer shredding system in a microcentrifuge spin-column format (QIAshredder, Qiagen) followed by total RNA purification (RNeasy Mini Kit, Qiagen). Method B: TRIzol RNA isolation (Invitrogen). Method C: TRIzol RNA isolation (Invitrogen) followed by an RNeasy purification step (RNeasy Mini Kit, Qiagen). The RNA purification step combines the selective binding properties of a silica-based membrane with the speed of microspin technology. It allows only RNA longer than 200 bases to bind to the silica membrane, providing an enriching for mRNA since nucleotides shorter than 200 nucleotides are selectively excluded. (B) For each of three additional samples, nine aliquots of mononuclear cells had been collected. Total RNA has been processed for each aliquot following one of the three methods and for each method three independent technical replicates were performed (A,A,A, B,B,B, C,C,C).

    Article Snippet: Method A: lysis of the mononuclear cells, followed by lysate homogenization using a biopolymer shredding system in a microcentrifuge spin-column format (QIAshredder, Qiagen, Hilden, Germany), followed by total RNA purification using selective binding columns (RNeasy Mini Kit, Qiagen).

    Techniques: Purification, Lysis, Homogenization, Molecular Weight, Isolation, Binding Assay