qiaquick pcr purification kit (Qiagen)

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Qiagen qiaquick pcr purification kit
Qiaquick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 13923 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qiaquick pcr purification kit/product/Qiagen
Average 99 stars, based on 13923 article reviews
Price from $9.99 to $1999.99

qiaquick pcr purification kit - by Bioz Stars, 2020-01

99/100 stars

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t4 dna ligase

Images

Clone Assay:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: Together with the subtype AG clone, this panel of clones represents 94% of worldwide HIV‐1 subtypes . .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen).

Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
Article Snippet: Some clones were further propagated on plates with 8 mM 2-FA and their APT locus was subsequently PCR amplified and sequenced to identify the mutation(s) responsible for the resistance. .. 71086), purified with the QIAquick PCR Purification Kit (Qiagen, cat. Nr.

Article Title: Expanded CTG repeat demarcates a boundary for abnormal CpG methylation in myotonic dystrophy patient tissues
Article Snippet: Detection of methylation was performed by direct PCR product sequencing as follows: first, if necessary (i.e. non-specific PCR products appearance), a step of gel extraction (QIAquick PCR purification kit, Qiagen) was performed; secondly, PCR product was cleaned (QIAquick PCR purification kit, Qiagen) and thirdly, samples were sent routinely to the sequencing facility of The Hospital for Sick Children (The Centre for Applied Genomics). .. Cloning of PCR products (TOPO-TA 4.0 cloning kit, Stratagene, USA) and at least 10 clones sequencing were performed in some mouse samples in order to validate the direct PCR sequencing methylation results ( ).

Amplification:

Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
Article Snippet: APT locus was amplified from isolated genomic DNA with KOD Hot Start DNA Polymerase (Millipore/Novagen, cat. Nr. .. 71086), purified with the QIAquick PCR Purification Kit (Qiagen, cat. Nr.

Article Title: Expanded CTG repeat demarcates a boundary for abnormal CpG methylation in myotonic dystrophy patient tissues
Article Snippet: An aliquot of 1–5 μl of bisulphate-treated DNAs was amplified in 25 or 50 μl PCR reactions with the corresponding primers. .. Detection of methylation was performed by direct PCR product sequencing as follows: first, if necessary (i.e. non-specific PCR products appearance), a step of gel extraction (QIAquick PCR purification kit, Qiagen) was performed; secondly, PCR product was cleaned (QIAquick PCR purification kit, Qiagen) and thirdly, samples were sent routinely to the sequencing facility of The Hospital for Sick Children (The Centre for Applied Genomics).

Polymerase Chain Reaction:

Article Title: Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis
Article Snippet: .. The resulting cDNA was purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany). cDNA was then subjected to end-repair and phosphorylation using T4 DNA polymerase, Klenow DNA polymerase and T4 PNK, and subsequent purification was performed using QIAquick PCR Purification Kit (Qiagen). .. These repaired cDNA fragments were 30-adenylated using Klenow Exo- (Illumina) and purified using MinElute PCR Purification Kit (Qiagen), producing cDNA fragments with a single ‘A’ base overhung at their 30-ends for subsequent adapter-ligation.

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
Article Snippet: .. 71086), purified with the QIAquick PCR Purification Kit (Qiagen, cat. Nr. .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

Article Title: Expanded CTG repeat demarcates a boundary for abnormal CpG methylation in myotonic dystrophy patient tissues
Article Snippet: .. Detection of methylation was performed by direct PCR product sequencing as follows: first, if necessary (i.e. non-specific PCR products appearance), a step of gel extraction (QIAquick PCR purification kit, Qiagen) was performed; secondly, PCR product was cleaned (QIAquick PCR purification kit, Qiagen) and thirdly, samples were sent routinely to the sequencing facility of The Hospital for Sick Children (The Centre for Applied Genomics). .. Additional quantification of the methylation profiles was performed in samples with only one DM1 allele in the genome (mouse model), by measuring in each CpG site the cytosine height peaks in the chromatogram traces (4Peaks software) ( ).

Construct:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: In order to construct the subtype reference plasmids, RNA was isolated from the viral reference culture supernatants with the Boom extraction method and used for RT‐PCR with Superscript‐III One‐Step Platinum Taq (Invitrogen, Carlsbad, CA, USA) and nested PCR with Platinum Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) using isolate‐specific primers (Tables ). .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen).

Transformation Assay:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

Bisulfite Sequencing:

Article Title: Expanded CTG repeat demarcates a boundary for abnormal CpG methylation in myotonic dystrophy patient tissues
Article Snippet: Paragraph title: CpG methylation analysis by bisulphite-sequencing PCR ... Detection of methylation was performed by direct PCR product sequencing as follows: first, if necessary (i.e. non-specific PCR products appearance), a step of gel extraction (QIAquick PCR purification kit, Qiagen) was performed; secondly, PCR product was cleaned (QIAquick PCR purification kit, Qiagen) and thirdly, samples were sent routinely to the sequencing facility of The Hospital for Sick Children (The Centre for Applied Genomics).

Ligation:

Article Title: Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis
Article Snippet: The resulting cDNA was purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany). cDNA was then subjected to end-repair and phosphorylation using T4 DNA polymerase, Klenow DNA polymerase and T4 PNK, and subsequent purification was performed using QIAquick PCR Purification Kit (Qiagen). .. To select a size range of templates for downstream enrichment, the products from ligation reactions were purified on a 2% TAE-agarose gel (Certified Low-Range Ultra Agarose, Biorad).

Methylation:

Cell Culture:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

CpG Methylation Assay:

Sequencing:

Article Title: Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis
Article Snippet: Paragraph title: Transcriptome sequencing and analysis ... The resulting cDNA was purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany). cDNA was then subjected to end-repair and phosphorylation using T4 DNA polymerase, Klenow DNA polymerase and T4 PNK, and subsequent purification was performed using QIAquick PCR Purification Kit (Qiagen).

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: The results were summarized by subtype or CRF and for each plasmid the sequence variants of the corresponding subtype or CRF were extracted. .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen).

Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
Article Snippet: 71086), purified with the QIAquick PCR Purification Kit (Qiagen, cat. Nr. .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

Magnetic Beads:

Article Title: Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis
Article Snippet: 10 mM Tris–HCl was used to elute the mRNA from the magnetic beads. .. The resulting cDNA was purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany). cDNA was then subjected to end-repair and phosphorylation using T4 DNA polymerase, Klenow DNA polymerase and T4 PNK, and subsequent purification was performed using QIAquick PCR Purification Kit (Qiagen).

Mutagenesis:

Isolation:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: In order to construct the subtype reference plasmids, RNA was isolated from the viral reference culture supernatants with the Boom extraction method and used for RT‐PCR with Superscript‐III One‐Step Platinum Taq (Invitrogen, Carlsbad, CA, USA) and nested PCR with Platinum Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) using isolate‐specific primers (Tables ). .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen).

Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
Article Snippet: Paragraph title: 2.5. Isolation and Analysis of apt Mutants after Bleomycin Treatment ... 71086), purified with the QIAquick PCR Purification Kit (Qiagen, cat. Nr.

Purification:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: In order to construct the subtype reference plasmids, RNA was isolated from the viral reference culture supernatants with the Boom extraction method and used for RT‐PCR with Superscript‐III One‐Step Platinum Taq (Invitrogen, Carlsbad, CA, USA) and nested PCR with Platinum Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) using isolate‐specific primers (Tables ). .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen).

Gel Extraction:

Nested PCR:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: In order to construct the subtype reference plasmids, RNA was isolated from the viral reference culture supernatants with the Boom extraction method and used for RT‐PCR with Superscript‐III One‐Step Platinum Taq (Invitrogen, Carlsbad, CA, USA) and nested PCR with Platinum Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) using isolate‐specific primers (Tables ). .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen).

Plasmid Preparation:

Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Article Snippet: .. Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

Software:

Article Title: Expanded CTG repeat demarcates a boundary for abnormal CpG methylation in myotonic dystrophy patient tissues
Article Snippet: Primers for bisulphate-modified DNA were designed using Methyl Primer Express Software v1.0 (Applied Biosystems, USA). .. Detection of methylation was performed by direct PCR product sequencing as follows: first, if necessary (i.e. non-specific PCR products appearance), a step of gel extraction (QIAquick PCR purification kit, Qiagen) was performed; secondly, PCR product was cleaned (QIAquick PCR purification kit, Qiagen) and thirdly, samples were sent routinely to the sequencing facility of The Hospital for Sick Children (The Centre for Applied Genomics).

Agarose Gel Electrophoresis:

Article Title: Expanded CTG repeat demarcates a boundary for abnormal CpG methylation in myotonic dystrophy patient tissues
Article Snippet: Products were resolved by running 2% agarose gel electrophoresis and visualized by ethidium bromide staining. .. Detection of methylation was performed by direct PCR product sequencing as follows: first, if necessary (i.e. non-specific PCR products appearance), a step of gel extraction (QIAquick PCR purification kit, Qiagen) was performed; secondly, PCR product was cleaned (QIAquick PCR purification kit, Qiagen) and thirdly, samples were sent routinely to the sequencing facility of The Hospital for Sick Children (The Centre for Applied Genomics).