qiaquick gel extraction kit  (Qiagen)


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    Name:
    QIAquick Gel Extraction Kit
    Description:
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    Catalog Number:
    28704
    Price:
    118
    Category:
    QIAquick Gel Extraction Kit
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    Structured Review

    Qiagen qiaquick gel extraction kit
    QIAquick Gel Extraction Kit
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/qiaquick gel extraction kit/product/Qiagen
    Average 90 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    qiaquick gel extraction kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Paragraph title: Cloning and sequence analysis of the pah1 gene. ... Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada).

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR amplifications of the ITS regions were performed using the forward primer 16S3′F ( ) and reverse primer 18m ( ).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: .. PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations. .. PCR products of Apobec-1 hepatic and small intestine RNA targets were concatemerized as follows.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing. .. Purified PCR products and plasmid DNA were sequenced using the BigDye Terminator v1.0 kit and an ABI PRISM 377 DNA sequencer (both from Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: For the Ddc probe, a 1.2-kb cDNA fragment spanning exons 6 to 15 was generated by PCR with the primers DDC-F8 (5′-CTGGGTTAATTGGTGGAATAAAGC-3′) and DDC-R8 (5′-TCTGAAGGTAAGACCAAAGACTGC-3′) and cloned into the pGEM-T vector (Promega). .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol.

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR products of about 600 bp were purified with a Qiaquick gel extraction kit (MinElute gel extraction kit; Qiagen) and commercially sequenced independently on both strands using primers 16S3′F, ALAF (5′-GGTTTAGCTCAGTTGGT-3′), and 18m.

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: Paragraph title: RNA Isolation and cDNA Cloning ... The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions.

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN). .. Size-purified PCR products were digested with EagI and cloned into the pZErO-2 vector (Invitrogen) at the NotI site.

    Amplification:

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: Paragraph title: Amplification of the 16S rRNA gene and ITS. ... After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′).

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: The ligation products were amplified by PCR using J-V-specific primers, nested with respect to the cDNA primer, and a 27-nt primer complementary in sequence to the anchor. .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing.

    Article Title: The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice
    Article Snippet: A complete list of amplification and sequencing primers is available from the authors upon request. .. DNA was recovered using the QiaQuick Gel Extraction Kit (Qiagen) as specified by the manufacturer.

    Article Title: Racial or ethnic differences in allele frequencies of single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase gene and their influence on response to methotrexate in rheumatoid arthritis
    Article Snippet: To allow simultaneous determination of genotypes at the rs1801131 A/C (1298) and rs2274976 T/C SNPs, we amplified by PCR a 152‐bp fragment from exon 7 of MTHFR . .. PCR products were analysed by electrophoresis on 2% agarose gel, followed by excision of bands from the gel and purification using the Qiaquick gel extraction kit (Qiagen, Valencia, California, USA).

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: IPMS2 was amplified from the first-strand cDNA product using the primer pair 1ipms2m/2ipms2n (for all primers used, see Supplemental Table S1), resulting in a truncated ORF lacking 138 nucleotides corresponding to a putative chloroplast transit peptide (ChloroP; ). .. The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions.

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: PCR amplification using GP5+/6+ biotinylated primers was carried out on plasmid dilutions and β globin positive clinical samples as follows. .. PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol.

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Two hybridizations were performed, after which tester-specific cDNA fragments were amplified by primary and nested PCR. .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN).

    Synthesized:

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: First-strand cDNA was synthesized with 2 μ g of total RNA, 200 units of MMLV reverse transcriptase (Promega), and 0.5 μ g of gene-specific oligonucleotide primer using the reagents and instructions provided. .. The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions.

    Nick Translation:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. Ampicillin-resistant transformants were screened by colony hybridization with a 0.46-kb Sal I fragment from the original pah gene which was 32 P labeled by nick translation for use as a probe.

    Construct:

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. As protein expression of the IPMS2/ pBAD-TOPO construct in BL21-CodonPlus-RIL cells (Stratagene) was very poor, IPMS2 was subcloned into the pCR-T7/CT-TOPO vector (Invitrogen).

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Electrophoresis:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Total genomic DNA from S. clavuligerus was digested to completion with Nco I, and the resulting fragments were separated by electrophoresis on a 0.8% agarose gel. .. Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada).

    Article Title: Racial or ethnic differences in allele frequencies of single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase gene and their influence on response to methotrexate in rheumatoid arthritis
    Article Snippet: .. PCR products were analysed by electrophoresis on 2% agarose gel, followed by excision of bands from the gel and purification using the Qiaquick gel extraction kit (Qiagen, Valencia, California, USA). .. Direct cycle sequencing of the amplified product was performed on an ABI 377 automated sequencer, using the Applied Biosystem BigDye™ Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems, Foster City, California, USA).

    Random Hexamer Labeling:

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Double-stranded cDNA was made using random hexamer oligonucleotide primers and total RNA from pelleted J-V (tester cDNA) and MoV (driver cDNA), respectively. .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN).

    Expressing:

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: .. The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. The DNA of transformed colonies was purified by a miniprep (Invisorb spin plasmid mini kit; Invitek) and screened by restriction analyses and DNA sequencing on an ABI 3700 DNA sequencer with Big Dye terminators (PE Applied Biosystems).

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Modification:

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: A slightly modified cDNA subtraction strategy was used for genomic subtraction. .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN).

    Transformation Assay:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. The purified DNA fragments were inserted into pUC120 digested with Nco I, and the ligation mixture was transformed into E. coli XL-1 Blue.

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: .. The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. The DNA of transformed colonies was purified by a miniprep (Invisorb spin plasmid mini kit; Invitek) and screened by restriction analyses and DNA sequencing on an ABI 3700 DNA sequencer with Big Dye terminators (PE Applied Biosystems).

    Derivative Assay:

    Article Title: The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice
    Article Snippet: Paragraph title: Consensus Sequencing of passage derived WNV ... DNA was recovered using the QiaQuick Gel Extraction Kit (Qiagen) as specified by the manufacturer.

    Hybridization:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. Ampicillin-resistant transformants were screened by colony hybridization with a 0.46-kb Sal I fragment from the original pah gene which was 32 P labeled by nick translation for use as a probe.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Digestion, adaptor ligation, hybridization, and PCRs were then carried out as described above. .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN).

    Southern Blot:

    Article Title: Evolution of mammalian CD1: marsupial CD1 is not orthologous to the eutherian isoforms and is a pseudogene in the opossum Monodelphis domestica
    Article Snippet: .. The 180-bp PCR product was excised from an agarose gel, gel-purified (QIAQuick Gel Extraction Kit, Qiagen, Valencia, CA) and used to probe the I. macrourus Southern blot. .. The probe was labelled with [32 P]dCTP by the random-prime-It RmT labelling kit (Stratagene, La Jolla, CA).

    Ligation:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. The purified DNA fragments were inserted into pUC120 digested with Nco I, and the ligation mixture was transformed into E. coli XL-1 Blue.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: The ligation products were amplified by PCR using J-V-specific primers, nested with respect to the cDNA primer, and a 27-nt primer complementary in sequence to the anchor. .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Digestion, adaptor ligation, hybridization, and PCRs were then carried out as described above. .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN).

    Northern Blot:

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: Paragraph title: Northern blotting. ... Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol.

    Introduce:

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Generated:

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: For the Ddc probe, a 1.2-kb cDNA fragment spanning exons 6 to 15 was generated by PCR with the primers DDC-F8 (5′-CTGGGTTAATTGGTGGAATAAAGC-3′) and DDC-R8 (5′-TCTGAAGGTAAGACCAAAGACTGC-3′) and cloned into the pGEM-T vector (Promega). .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol.

    DNA Sequencing:

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. The DNA of transformed colonies was purified by a miniprep (Invisorb spin plasmid mini kit; Invitek) and screened by restriction analyses and DNA sequencing on an ABI 3700 DNA sequencer with Big Dye terminators (PE Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes
    Article Snippet: .. B. burgdorferi ospC PCR products (25 μl) were electrophoresed in 2% agarose gels, stained with ethidium bromide, and excised for purification using a QIAquick gel extraction kit (QIAGEN Inc.). .. Sequencing was performed using the forward and reverse PCR primers ( ) and a fluorescent automated method (ABI 3100 genetic analyzer).

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR amplifications of the ITS regions were performed using the forward primer 16S3′F ( ) and reverse primer 18m ( ).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: .. PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations. .. PCR products of Apobec-1 hepatic and small intestine RNA targets were concatemerized as follows.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing. .. Purified PCR products and plasmid DNA were sequenced using the BigDye Terminator v1.0 kit and an ABI PRISM 377 DNA sequencer (both from Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: For the Ddc probe, a 1.2-kb cDNA fragment spanning exons 6 to 15 was generated by PCR with the primers DDC-F8 (5′-CTGGGTTAATTGGTGGAATAAAGC-3′) and DDC-R8 (5′-TCTGAAGGTAAGACCAAAGACTGC-3′) and cloned into the pGEM-T vector (Promega). .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol.

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. PCR products of about 600 bp were purified with a Qiaquick gel extraction kit (MinElute gel extraction kit; Qiagen) and commercially sequenced independently on both strands using primers 16S3′F, ALAF (5′-GGTTTAGCTCAGTTGGT-3′), and 18m. ..

    Article Title: Racial or ethnic differences in allele frequencies of single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase gene and their influence on response to methotrexate in rheumatoid arthritis
    Article Snippet: .. PCR products were analysed by electrophoresis on 2% agarose gel, followed by excision of bands from the gel and purification using the Qiaquick gel extraction kit (Qiagen, Valencia, California, USA). .. Direct cycle sequencing of the amplified product was performed on an ABI 377 automated sequencer, using the Applied Biosystem BigDye™ Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems, Foster City, California, USA).

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. As protein expression of the IPMS2/ pBAD-TOPO construct in BL21-CodonPlus-RIL cells (Stratagene) was very poor, IPMS2 was subcloned into the pCR-T7/CT-TOPO vector (Invitrogen).

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: .. PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol. .. Products were analysed on a Perkin Elmer 377 ABI Prism automated sequencer and BioEdit Sequence Alignment software was used to align the forward and reverse complement sequences.

    Article Title: Epigenetic Effects of the Continuous Exposure to Peroxisome Proliferator WY-14,643 in Mouse Liver are Dependent upon Peroxisome Proliferator Activated Receptor ?
    Article Snippet: Paragraph title: Methylation-sensitive arbitrarily primed PCR ... McrBC-digested DNA fragments were separated on a 1% agarose gel, and DNA fragments larger than 1 kb were excised from the gel and purified by using a QIAquick Gel Extraction kit according to manufacturer's protocol (Qiagen, Valencia, CA).

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Article Title: Evolution of mammalian CD1: marsupial CD1 is not orthologous to the eutherian isoforms and is a pseudogene in the opossum Monodelphis domestica
    Article Snippet: .. The 180-bp PCR product was excised from an agarose gel, gel-purified (QIAQuick Gel Extraction Kit, Qiagen, Valencia, CA) and used to probe the I. macrourus Southern blot. .. The probe was labelled with [32 P]dCTP by the random-prime-It RmT labelling kit (Stratagene, La Jolla, CA).

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN). .. Size-purified PCR products were digested with EagI and cloned into the pZErO-2 vector (Invitrogen) at the NotI site.

    Methylation:

    Article Title: Epigenetic Effects of the Continuous Exposure to Peroxisome Proliferator WY-14,643 in Mouse Liver are Dependent upon Peroxisome Proliferator Activated Receptor ?
    Article Snippet: Paragraph title: Methylation-sensitive arbitrarily primed PCR ... McrBC-digested DNA fragments were separated on a 1% agarose gel, and DNA fragments larger than 1 kb were excised from the gel and purified by using a QIAquick Gel Extraction kit according to manufacturer's protocol (Qiagen, Valencia, CA).

    Mutagenesis:

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: The vpu mutant clone pMJ4-Vpu-S58,62N was constructed using QuikChange. .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB).

    Isolation:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. Plasmid DNA was isolated from hybridizing transformants and was confirmed to carry a 4.3-kb Nco I fragment.

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol. .. Hybridization intensity signals were quantified with a FLA-3000 PhosphorImager system (Fujifilm).

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: Paragraph title: RNA Isolation and cDNA Cloning ... The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Paragraph title: Isolation and characterization of viral cDNA using cDNA subtraction. ... Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN).

    Labeling:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. Ampicillin-resistant transformants were screened by colony hybridization with a 0.46-kb Sal I fragment from the original pah gene which was 32 P labeled by nick translation for use as a probe.

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol. .. Hybridization intensity signals were quantified with a FLA-3000 PhosphorImager system (Fujifilm).

    Purification:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. The purified DNA fragments were inserted into pUC120 digested with Nco I, and the ligation mixture was transformed into E. coli XL-1 Blue.

    Article Title: Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes
    Article Snippet: .. B. burgdorferi ospC PCR products (25 μl) were electrophoresed in 2% agarose gels, stained with ethidium bromide, and excised for purification using a QIAquick gel extraction kit (QIAGEN Inc.). .. Sequencing was performed using the forward and reverse PCR primers ( ) and a fluorescent automated method (ABI 3100 genetic analyzer).

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR amplifications of the ITS regions were performed using the forward primer 16S3′F ( ) and reverse primer 18m ( ).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: .. PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations. .. PCR products of Apobec-1 hepatic and small intestine RNA targets were concatemerized as follows.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing. .. Purified PCR products and plasmid DNA were sequenced using the BigDye Terminator v1.0 kit and an ABI PRISM 377 DNA sequencer (both from Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol. .. Hybridization intensity signals were quantified with a FLA-3000 PhosphorImager system (Fujifilm).

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. PCR products of about 600 bp were purified with a Qiaquick gel extraction kit (MinElute gel extraction kit; Qiagen) and commercially sequenced independently on both strands using primers 16S3′F, ALAF (5′-GGTTTAGCTCAGTTGGT-3′), and 18m. ..

    Article Title: Racial or ethnic differences in allele frequencies of single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase gene and their influence on response to methotrexate in rheumatoid arthritis
    Article Snippet: .. PCR products were analysed by electrophoresis on 2% agarose gel, followed by excision of bands from the gel and purification using the Qiaquick gel extraction kit (Qiagen, Valencia, California, USA). .. Direct cycle sequencing of the amplified product was performed on an ABI 377 automated sequencer, using the Applied Biosystem BigDye™ Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems, Foster City, California, USA).

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: .. The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. The DNA of transformed colonies was purified by a miniprep (Invisorb spin plasmid mini kit; Invitek) and screened by restriction analyses and DNA sequencing on an ABI 3700 DNA sequencer with Big Dye terminators (PE Applied Biosystems).

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: .. PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol. .. Products were analysed on a Perkin Elmer 377 ABI Prism automated sequencer and BioEdit Sequence Alignment software was used to align the forward and reverse complement sequences.

    Article Title: Epigenetic Effects of the Continuous Exposure to Peroxisome Proliferator WY-14,643 in Mouse Liver are Dependent upon Peroxisome Proliferator Activated Receptor ?
    Article Snippet: .. McrBC-digested DNA fragments were separated on a 1% agarose gel, and DNA fragments larger than 1 kb were excised from the gel and purified by using a QIAquick Gel Extraction kit according to manufacturer's protocol (Qiagen, Valencia, CA). .. The fragments, which were enriched for unmethylated DNA, were then digested overnight with 20 U/μg DNA of methylation-sensitive restriction endonuclease SmaI (New England Biolabs), followed by digestion with 20 U/μg DNA of HpaII (New England Biolabs).

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN). .. Size-purified PCR products were digested with EagI and cloned into the pZErO-2 vector (Invitrogen) at the NotI site.

    Sequencing:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Paragraph title: Cloning and sequence analysis of the pah1 gene. ... Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada).

    Article Title: Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes
    Article Snippet: Paragraph title: Sequencing and phylogenetic analysis. ... B. burgdorferi ospC PCR products (25 μl) were electrophoresed in 2% agarose gels, stained with ethidium bromide, and excised for purification using a QIAquick gel extraction kit (QIAGEN Inc.).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: Paragraph title: RNA editing analysis by Sanger sequencing ... PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing. .. Purified PCR products and plasmid DNA were sequenced using the BigDye Terminator v1.0 kit and an ABI PRISM 377 DNA sequencer (both from Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice
    Article Snippet: Paragraph title: Consensus Sequencing of passage derived WNV ... DNA was recovered using the QiaQuick Gel Extraction Kit (Qiagen) as specified by the manufacturer.

    Article Title: Racial or ethnic differences in allele frequencies of single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase gene and their influence on response to methotrexate in rheumatoid arthritis
    Article Snippet: PCR products were analysed by electrophoresis on 2% agarose gel, followed by excision of bands from the gel and purification using the Qiaquick gel extraction kit (Qiagen, Valencia, California, USA). .. Direct cycle sequencing of the amplified product was performed on an ABI 377 automated sequencer, using the Applied Biosystem BigDye™ Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems, Foster City, California, USA).

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: .. PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol. .. Products were analysed on a Perkin Elmer 377 ABI Prism automated sequencer and BioEdit Sequence Alignment software was used to align the forward and reverse complement sequences.

    Staining:

    Article Title: Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes
    Article Snippet: .. B. burgdorferi ospC PCR products (25 μl) were electrophoresed in 2% agarose gels, stained with ethidium bromide, and excised for purification using a QIAquick gel extraction kit (QIAGEN Inc.). .. Sequencing was performed using the forward and reverse PCR primers ( ) and a fluorescent automated method (ABI 3100 genetic analyzer).

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: Aliquots (5 μl) from each PCR reaction were run on a 2% agarose gel, stained with 0.3 μg/ml ethidium bromide, and visualised on an ultraviolet imager. .. PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol.

    Nested PCR:

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN). .. Size-purified PCR products were digested with EagI and cloned into the pZErO-2 vector (Invitrogen) at the NotI site.

    Activated Clotting Time Assay:

    Article Title: Epigenetic Effects of the Continuous Exposure to Peroxisome Proliferator WY-14,643 in Mouse Liver are Dependent upon Peroxisome Proliferator Activated Receptor ?
    Article Snippet: McrBC is a methylation-specific endonuclease, which, as opposed to methylation-sensitive restriction endonucleases, cleaves DNA containing 5-methylcytosine on one or both strands but does not act on unmethylated DNA. .. McrBC-digested DNA fragments were separated on a 1% agarose gel, and DNA fragments larger than 1 kb were excised from the gel and purified by using a QIAquick Gel Extraction kit according to manufacturer's protocol (Qiagen, Valencia, CA).

    Rapid Amplification of cDNA Ends:

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: A protocol adapted from a published 5′ rapid amplification of cDNA ends (5′ RACE) method ( ) was used to obtain the sequence for 117 nt at the 3′ end of the J-V genome and the exact sequence of the 13 nt at the 5′ genome terminus. .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing.

    Plasmid Preparation:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. Plasmid DNA was isolated from hybridizing transformants and was confirmed to carry a 4.3-kb Nco I fragment.

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR amplifications of the ITS regions were performed using the forward primer 16S3′F ( ) and reverse primer 18m ( ).

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing. .. Purified PCR products and plasmid DNA were sequenced using the BigDye Terminator v1.0 kit and an ABI PRISM 377 DNA sequencer (both from Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: For the Ddc probe, a 1.2-kb cDNA fragment spanning exons 6 to 15 was generated by PCR with the primers DDC-F8 (5′-CTGGGTTAATTGGTGGAATAAAGC-3′) and DDC-R8 (5′-TCTGAAGGTAAGACCAAAGACTGC-3′) and cloned into the pGEM-T vector (Promega). .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol.

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR products of about 600 bp were purified with a Qiaquick gel extraction kit (MinElute gel extraction kit; Qiagen) and commercially sequenced independently on both strands using primers 16S3′F, ALAF (5′-GGTTTAGCTCAGTTGGT-3′), and 18m.

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: .. The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. The DNA of transformed colonies was purified by a miniprep (Invisorb spin plasmid mini kit; Invitek) and screened by restriction analyses and DNA sequencing on an ABI 3700 DNA sequencer with Big Dye terminators (PE Applied Biosystems).

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: Briefly, PCR was performed in a final reaction volume of 50 μl containing either 10 μl of diluted plasmid DNA with 200 ng human placental DNA or 200 ng genomic DNA, 5 μl buffer II 10× (supplied with AmpliTaq Gold; Applied Biosystems, Foster City, California, USA), 3.5mM MgCl2 , 50 μM of each deoxynucleoside triphosphate, 50 pmole of each forward and reverse primer, and 1 U of AmpliTaq Gold. .. PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol.

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN). .. Size-purified PCR products were digested with EagI and cloned into the pZErO-2 vector (Invitrogen) at the NotI site.

    Software:

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol. .. Products were analysed on a Perkin Elmer 377 ABI Prism automated sequencer and BioEdit Sequence Alignment software was used to align the forward and reverse complement sequences.

    Agarose Gel Electrophoresis:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: Total genomic DNA from S. clavuligerus was digested to completion with Nco I, and the resulting fragments were separated by electrophoresis on a 0.8% agarose gel. .. Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada).

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR amplifications of the ITS regions were performed using the forward primer 16S3′F ( ) and reverse primer 18m ( ).

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR products of about 600 bp were purified with a Qiaquick gel extraction kit (MinElute gel extraction kit; Qiagen) and commercially sequenced independently on both strands using primers 16S3′F, ALAF (5′-GGTTTAGCTCAGTTGGT-3′), and 18m.

    Article Title: Racial or ethnic differences in allele frequencies of single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase gene and their influence on response to methotrexate in rheumatoid arthritis
    Article Snippet: .. PCR products were analysed by electrophoresis on 2% agarose gel, followed by excision of bands from the gel and purification using the Qiaquick gel extraction kit (Qiagen, Valencia, California, USA). .. Direct cycle sequencing of the amplified product was performed on an ABI 377 automated sequencer, using the Applied Biosystem BigDye™ Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems, Foster City, California, USA).

    Article Title: Epigenetic Effects of the Continuous Exposure to Peroxisome Proliferator WY-14,643 in Mouse Liver are Dependent upon Peroxisome Proliferator Activated Receptor ?
    Article Snippet: .. McrBC-digested DNA fragments were separated on a 1% agarose gel, and DNA fragments larger than 1 kb were excised from the gel and purified by using a QIAquick Gel Extraction kit according to manufacturer's protocol (Qiagen, Valencia, CA). .. The fragments, which were enriched for unmethylated DNA, were then digested overnight with 20 U/μg DNA of methylation-sensitive restriction endonuclease SmaI (New England Biolabs), followed by digestion with 20 U/μg DNA of HpaII (New England Biolabs).

    Article Title: Evolution of mammalian CD1: marsupial CD1 is not orthologous to the eutherian isoforms and is a pseudogene in the opossum Monodelphis domestica
    Article Snippet: .. The 180-bp PCR product was excised from an agarose gel, gel-purified (QIAQuick Gel Extraction Kit, Qiagen, Valencia, CA) and used to probe the I. macrourus Southern blot. .. The probe was labelled with [32 P]dCTP by the random-prime-It RmT labelling kit (Stratagene, La Jolla, CA).

    Concentration Assay:

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: PCRs were carried out in 25-μl aliquots containing approximately 100 ng DNA, a deoxynucleoside triphosphate mixture (0.2 mM each), buffer (1/10 volume of the supplied 10× buffer) supplemented to give a final concentration of 3 mM MgCl2 , 1 U of Taq polymerase (Amersham, Pharmacia), and 0.5 pmol of each primer. .. After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′).

    FLAG-tag:

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Gel Extraction:

    Article Title: Streptomyces clavuligerus Has a Second Copy of the Proclavaminate Amidinohydrolase Gene
    Article Snippet: .. Regions of the gel corresponding to DNA fragments of 4 to 5 kb were excised with a sharp blade, and the DNA was recovered by using a QIAquick gel extraction kit (Qiagen, Mississauga, Ontario, Canada). .. The purified DNA fragments were inserted into pUC120 digested with Nco I, and the ligation mixture was transformed into E. coli XL-1 Blue.

    Article Title: Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes
    Article Snippet: .. B. burgdorferi ospC PCR products (25 μl) were electrophoresed in 2% agarose gels, stained with ethidium bromide, and excised for purification using a QIAquick gel extraction kit (QIAGEN Inc.). .. Sequencing was performed using the forward and reverse PCR primers ( ) and a fluorescent automated method (ABI 3100 genetic analyzer).

    Article Title: Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea ▿
    Article Snippet: .. After purification from the agarose gel using a Qiaquick gel extraction kit (Qiagen), the PCR products (∼1,100 bp) were cloned into pGEM-T Easy vector (Promega) and sequenced using primers CYA359, C, AC552F (5′-CAGCCGCGGTAATAC-3′), and AC552R (5′-GTATTACCGCGGCTG-3′). .. PCR amplifications of the ITS regions were performed using the forward primer 16S3′F ( ) and reverse primer 18m ( ).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: .. PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations. .. PCR products of Apobec-1 hepatic and small intestine RNA targets were concatemerized as follows.

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. PCR products obtained were gel purified using the QIAquick Gel Extraction kit (QIAGEN) and were either sequenced directly or cloned into the pDrive vector (QIAGEN) for sequencing. .. Purified PCR products and plasmid DNA were sequenced using the BigDye Terminator v1.0 kit and an ABI PRISM 377 DNA sequencer (both from Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice
    Article Snippet: .. DNA was recovered using the QiaQuick Gel Extraction Kit (Qiagen) as specified by the manufacturer. .. Full length WNV consensus sequencing was carried out using multiple pairs of overlapping primers and was performed at the WCMGC as previously described ( ).

    Article Title: Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10 ▿
    Article Snippet: .. Probe template DNA was then isolated from pGEM-T by digestion with NotI, purified with the QiaQuick gel extraction kit (Qiagen), and used to generate [α-32 P]dCTP-labeled probe with the Hi-prime random prime labeling kit (Roche) according to the manufacturer's protocol. .. Hybridization intensity signals were quantified with a FLA-3000 PhosphorImager system (Fujifilm).

    Article Title: Racial or ethnic differences in allele frequencies of single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase gene and their influence on response to methotrexate in rheumatoid arthritis
    Article Snippet: .. PCR products were analysed by electrophoresis on 2% agarose gel, followed by excision of bands from the gel and purification using the Qiaquick gel extraction kit (Qiagen, Valencia, California, USA). .. Direct cycle sequencing of the amplified product was performed on an ABI 377 automated sequencer, using the Applied Biosystem BigDye™ Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems, Foster City, California, USA).

    Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
    Article Snippet: .. The reaction product was gel purified using a QiaQuick gel extraction kit (Qiagen), cloned directly into the pBAD-TOPO (Invitrogen) expression vector, and transformed into TOP10 cells (Invitrogen) according to the manufacturer's instructions. .. The DNA of transformed colonies was purified by a miniprep (Invisorb spin plasmid mini kit; Invitek) and screened by restriction analyses and DNA sequencing on an ABI 3700 DNA sequencer with Big Dye terminators (PE Applied Biosystems).

    Article Title: Does human papillomavirus play a role in the development of bladder transitional cell carcinoma? A comparison of PCR and immunohistochemical analysis
    Article Snippet: .. PCR products of positive samples were gel purified using a QIAquick gel extraction kit (Qiagen) and sequenced using an ABI Prism dRhodamine terminator cycle sequencing kit (Applied Biosystems), according to the manufacturer’s protocol. .. Products were analysed on a Perkin Elmer 377 ABI Prism automated sequencer and BioEdit Sequence Alignment software was used to align the forward and reverse complement sequences.

    Article Title: Epigenetic Effects of the Continuous Exposure to Peroxisome Proliferator WY-14,643 in Mouse Liver are Dependent upon Peroxisome Proliferator Activated Receptor ?
    Article Snippet: .. McrBC-digested DNA fragments were separated on a 1% agarose gel, and DNA fragments larger than 1 kb were excised from the gel and purified by using a QIAquick Gel Extraction kit according to manufacturer's protocol (Qiagen, Valencia, CA). .. The fragments, which were enriched for unmethylated DNA, were then digested overnight with 20 U/μg DNA of methylation-sensitive restriction endonuclease SmaI (New England Biolabs), followed by digestion with 20 U/μg DNA of HpaII (New England Biolabs).

    Article Title: The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release
    Article Snippet: .. For the Rev-dependent FLAG-tagged MJ4-Vpu expression construct, the vpu coding region from pMJ4 was amplified by PCR with primers designed to introduce a C-terminal FLAG tag, gel purified using a QIAquick gel extraction kit (Qiagen), and then cloned into the pcDNA-RRE expression vector (described in reference ) after digestion of the purified PCR products and backbone with NheI and XhoI (NEB). .. Expression plasmids encoding Vpu-L78A, Vpu-R79A, Vpu-L80A, Vpu-L81A, Vpu L78A,R79A (LR/AA), Vpu-L80A,L81A (LL/AA), Vpu-L78A,R79A,L80A,L81A (LRLL/AAAA), and Vpu-L78H,R79A,L80P,L81W (LRLL/HAPW) with a C-terminal FLAG tag were made by QuikChange site-directed mutagenesis (Agilent) of the MJ4-Vpu-FLAG expression construct.

    Article Title: Evolution of mammalian CD1: marsupial CD1 is not orthologous to the eutherian isoforms and is a pseudogene in the opossum Monodelphis domestica
    Article Snippet: .. The 180-bp PCR product was excised from an agarose gel, gel-purified (QIAQuick Gel Extraction Kit, Qiagen, Valencia, CA) and used to probe the I. macrourus Southern blot. .. The probe was labelled with [32 P]dCTP by the random-prime-It RmT labelling kit (Stratagene, La Jolla, CA).

    Article Title: The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
    Article Snippet: .. Nested PCR products from both subtractions were electrophoresed on 1% agarose gels and subsequently size purified in three fractions (0.2 to 0.6 kb, 0.6 to 1.0 kb, and 1.0 to 2.0 kb) using the QIAquick Gel Extraction kit (QIAGEN). .. Size-purified PCR products were digested with EagI and cloned into the pZErO-2 vector (Invitrogen) at the NotI site.

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    Qiagen qiaquick gel extractio n kit
    Qiaquick Gel Extractio N Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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