qiaquick extration gel kit  (Qiagen)

 
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    Name:
    QIAquick Gel Extraction Kit
    Description:
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    Catalog Number:
    28704
    Price:
    118
    Category:
    QIAquick Gel Extraction Kit
    Buy from Supplier


    Structured Review

    Qiagen qiaquick extration gel kit
    QIAquick Gel Extraction Kit
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/qiaquick extration gel kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaquick extration gel kit - by Bioz Stars, 2020-09
    99/100 stars

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    Clone Assay:

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Article Title: Utility of arsenic-treated bird skins for DNA extraction
    Article Snippet: .. All 600 bp PCR fragments were purified from agarose gels using the Qiaquick Gel Extraction Kit (Qiagen) and cloned (TOPO TA Cloning Kit, Invitrogen) prior to sequencing. .. Sequencing of cloned PCR products (both strands) was performed with universal M13 primers by AGOWA.

    Amplification:

    Article Title: Genotype V Japanese Encephalitis Virus Is Emerging
    Article Snippet: .. The programme ended with an extension step at 72°C for 10 min. Amplified products were examined by agarose gel electrophoresis (1%), purified using a QIAquick Gel Extraction kit (QIAGEN, Germany), and then sequenced directly. .. Sequencing of the 5′ UTR and 3′ UTR were determined by using 5′ RACE and 3′ RACE system for Rapid Amplification of cDNA Ends (Invitrogen) respectively.

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    Agarose Gel Electrophoresis:

    Article Title: Gene Constellation of Influenza A Virus Reassortants with High Growth Phenotype Prepared as Seed Candidates for Vaccine Production
    Article Snippet: .. DNA purification PCR products were gel purified in a 2% low melt agarose gel using QIAquick® Gel Extraction Kit (Qiagen Inc., Valencia, CA) per manufacturer's recommendations. .. The extracted PCR products were visualized for purity on a 2% agarose-TAE/EtBr gel.

    Article Title: Genotype V Japanese Encephalitis Virus Is Emerging
    Article Snippet: .. The programme ended with an extension step at 72°C for 10 min. Amplified products were examined by agarose gel electrophoresis (1%), purified using a QIAquick Gel Extraction kit (QIAGEN, Germany), and then sequenced directly. .. Sequencing of the 5′ UTR and 3′ UTR were determined by using 5′ RACE and 3′ RACE system for Rapid Amplification of cDNA Ends (Invitrogen) respectively.

    Activation Assay:

    Article Title: Optimized design of antisense oligomers for targeted rRNA depletion
    Article Snippet: .. Initial samples for the X. laevis 28S qRT-PCR were column purified (Qiagen #28704) and used at full concentration for qRT-PCR; subsequent samples were used directly at 1:10 dilution for qRT-PCR based on the results of a 4-sample, 1:5 dilution calibration curve analysis. qRT-PCR was performed in triplicate using 10μl reactions (2.5μl of cDNA, 5 μM of each forward and reverse primers, and 2x SYGreen mix (Genesee #17-505B)). qPCR was performed on QuantStudio 3 (Applied Biosystems) with an initial heat activation at 50°C for 2 minutes and then 95°C for 10 minutes. ..

    TA Cloning:

    Article Title: Utility of arsenic-treated bird skins for DNA extraction
    Article Snippet: .. All 600 bp PCR fragments were purified from agarose gels using the Qiaquick Gel Extraction Kit (Qiagen) and cloned (TOPO TA Cloning Kit, Invitrogen) prior to sequencing. .. Sequencing of cloned PCR products (both strands) was performed with universal M13 primers by AGOWA.

    Quantitative RT-PCR:

    Article Title: Optimized design of antisense oligomers for targeted rRNA depletion
    Article Snippet: .. Initial samples for the X. laevis 28S qRT-PCR were column purified (Qiagen #28704) and used at full concentration for qRT-PCR; subsequent samples were used directly at 1:10 dilution for qRT-PCR based on the results of a 4-sample, 1:5 dilution calibration curve analysis. qRT-PCR was performed in triplicate using 10μl reactions (2.5μl of cDNA, 5 μM of each forward and reverse primers, and 2x SYGreen mix (Genesee #17-505B)). qPCR was performed on QuantStudio 3 (Applied Biosystems) with an initial heat activation at 50°C for 2 minutes and then 95°C for 10 minutes. ..

    Purification:

    Article Title: Gene Constellation of Influenza A Virus Reassortants with High Growth Phenotype Prepared as Seed Candidates for Vaccine Production
    Article Snippet: .. DNA purification PCR products were gel purified in a 2% low melt agarose gel using QIAquick® Gel Extraction Kit (Qiagen Inc., Valencia, CA) per manufacturer's recommendations. .. The extracted PCR products were visualized for purity on a 2% agarose-TAE/EtBr gel.

    Article Title: Genotype V Japanese Encephalitis Virus Is Emerging
    Article Snippet: .. The programme ended with an extension step at 72°C for 10 min. Amplified products were examined by agarose gel electrophoresis (1%), purified using a QIAquick Gel Extraction kit (QIAGEN, Germany), and then sequenced directly. .. Sequencing of the 5′ UTR and 3′ UTR were determined by using 5′ RACE and 3′ RACE system for Rapid Amplification of cDNA Ends (Invitrogen) respectively.

    Article Title: Recombining overlapping BACs into a single larger BAC
    Article Snippet: .. For recombination with pBACLinkSpAB and pBACLinkGmBC, Not I linearized DNA was gel purified using the Qiaquick Gel Extraction Kit (Qiagen). .. After electroporation as described above, the bacteria were spread on plates containing 50 μg/ml of spectinomycin or 5 μg/ml gentamycin respectively.

    Article Title: Utility of arsenic-treated bird skins for DNA extraction
    Article Snippet: .. All 600 bp PCR fragments were purified from agarose gels using the Qiaquick Gel Extraction Kit (Qiagen) and cloned (TOPO TA Cloning Kit, Invitrogen) prior to sequencing. .. Sequencing of cloned PCR products (both strands) was performed with universal M13 primers by AGOWA.

    Article Title: Optimized design of antisense oligomers for targeted rRNA depletion
    Article Snippet: .. Initial samples for the X. laevis 28S qRT-PCR were column purified (Qiagen #28704) and used at full concentration for qRT-PCR; subsequent samples were used directly at 1:10 dilution for qRT-PCR based on the results of a 4-sample, 1:5 dilution calibration curve analysis. qRT-PCR was performed in triplicate using 10μl reactions (2.5μl of cDNA, 5 μM of each forward and reverse primers, and 2x SYGreen mix (Genesee #17-505B)). qPCR was performed on QuantStudio 3 (Applied Biosystems) with an initial heat activation at 50°C for 2 minutes and then 95°C for 10 minutes. ..

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    Article Title: A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
    Article Snippet: .. The P1316 plasmid was also NotI/AscI digested and gel purified (Qiagen/QiaQuick Gel Extraction Cat# 28706). .. P1316 is a derivative of the pTT3 expression vector ( ) and consists of (5’ to 3’): a CMV promoter, the mouse V kappa leader sequence, a NotI restriction site, an insert consisting of VL /joining fragment/VH , and the mouse IgG1 CH sequence amplified from mouse genomic DNA, flanked by AscI and XbaI restriction sites ( ; ).

    Real-time Polymerase Chain Reaction:

    Article Title: Optimized design of antisense oligomers for targeted rRNA depletion
    Article Snippet: .. Initial samples for the X. laevis 28S qRT-PCR were column purified (Qiagen #28704) and used at full concentration for qRT-PCR; subsequent samples were used directly at 1:10 dilution for qRT-PCR based on the results of a 4-sample, 1:5 dilution calibration curve analysis. qRT-PCR was performed in triplicate using 10μl reactions (2.5μl of cDNA, 5 μM of each forward and reverse primers, and 2x SYGreen mix (Genesee #17-505B)). qPCR was performed on QuantStudio 3 (Applied Biosystems) with an initial heat activation at 50°C for 2 minutes and then 95°C for 10 minutes. ..

    Concentration Assay:

    Article Title: Optimized design of antisense oligomers for targeted rRNA depletion
    Article Snippet: .. Initial samples for the X. laevis 28S qRT-PCR were column purified (Qiagen #28704) and used at full concentration for qRT-PCR; subsequent samples were used directly at 1:10 dilution for qRT-PCR based on the results of a 4-sample, 1:5 dilution calibration curve analysis. qRT-PCR was performed in triplicate using 10μl reactions (2.5μl of cDNA, 5 μM of each forward and reverse primers, and 2x SYGreen mix (Genesee #17-505B)). qPCR was performed on QuantStudio 3 (Applied Biosystems) with an initial heat activation at 50°C for 2 minutes and then 95°C for 10 minutes. ..

    Polymerase Chain Reaction:

    Article Title: Gene Constellation of Influenza A Virus Reassortants with High Growth Phenotype Prepared as Seed Candidates for Vaccine Production
    Article Snippet: .. DNA purification PCR products were gel purified in a 2% low melt agarose gel using QIAquick® Gel Extraction Kit (Qiagen Inc., Valencia, CA) per manufacturer's recommendations. .. The extracted PCR products were visualized for purity on a 2% agarose-TAE/EtBr gel.

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Article Title: Utility of arsenic-treated bird skins for DNA extraction
    Article Snippet: .. All 600 bp PCR fragments were purified from agarose gels using the Qiaquick Gel Extraction Kit (Qiagen) and cloned (TOPO TA Cloning Kit, Invitrogen) prior to sequencing. .. Sequencing of cloned PCR products (both strands) was performed with universal M13 primers by AGOWA.

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    DNA Purification:

    Article Title: Gene Constellation of Influenza A Virus Reassortants with High Growth Phenotype Prepared as Seed Candidates for Vaccine Production
    Article Snippet: .. DNA purification PCR products were gel purified in a 2% low melt agarose gel using QIAquick® Gel Extraction Kit (Qiagen Inc., Valencia, CA) per manufacturer's recommendations. .. The extracted PCR products were visualized for purity on a 2% agarose-TAE/EtBr gel.

    CRISPR:

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Sequencing:

    Article Title: Utility of arsenic-treated bird skins for DNA extraction
    Article Snippet: .. All 600 bp PCR fragments were purified from agarose gels using the Qiaquick Gel Extraction Kit (Qiagen) and cloned (TOPO TA Cloning Kit, Invitrogen) prior to sequencing. .. Sequencing of cloned PCR products (both strands) was performed with universal M13 primers by AGOWA.

    Gel Extraction:

    Article Title: Gene Constellation of Influenza A Virus Reassortants with High Growth Phenotype Prepared as Seed Candidates for Vaccine Production
    Article Snippet: .. DNA purification PCR products were gel purified in a 2% low melt agarose gel using QIAquick® Gel Extraction Kit (Qiagen Inc., Valencia, CA) per manufacturer's recommendations. .. The extracted PCR products were visualized for purity on a 2% agarose-TAE/EtBr gel.

    Article Title: Genotype V Japanese Encephalitis Virus Is Emerging
    Article Snippet: .. The programme ended with an extension step at 72°C for 10 min. Amplified products were examined by agarose gel electrophoresis (1%), purified using a QIAquick Gel Extraction kit (QIAGEN, Germany), and then sequenced directly. .. Sequencing of the 5′ UTR and 3′ UTR were determined by using 5′ RACE and 3′ RACE system for Rapid Amplification of cDNA Ends (Invitrogen) respectively.

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

    Article Title: Recombining overlapping BACs into a single larger BAC
    Article Snippet: .. For recombination with pBACLinkSpAB and pBACLinkGmBC, Not I linearized DNA was gel purified using the Qiaquick Gel Extraction Kit (Qiagen). .. After electroporation as described above, the bacteria were spread on plates containing 50 μg/ml of spectinomycin or 5 μg/ml gentamycin respectively.

    Article Title: Utility of arsenic-treated bird skins for DNA extraction
    Article Snippet: .. All 600 bp PCR fragments were purified from agarose gels using the Qiaquick Gel Extraction Kit (Qiagen) and cloned (TOPO TA Cloning Kit, Invitrogen) prior to sequencing. .. Sequencing of cloned PCR products (both strands) was performed with universal M13 primers by AGOWA.

    Article Title: High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
    Article Snippet: .. The amplified DNAs were purified from agarose gels using a commercial kit (QIAquik Gel Extraction Kit protocol, QIAGEN) and the amplicons were sequenced in both senses using the internal PCR primers. ..

    Article Title: A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
    Article Snippet: .. The P1316 plasmid was also NotI/AscI digested and gel purified (Qiagen/QiaQuick Gel Extraction Cat# 28706). .. P1316 is a derivative of the pTT3 expression vector ( ) and consists of (5’ to 3’): a CMV promoter, the mouse V kappa leader sequence, a NotI restriction site, an insert consisting of VL /joining fragment/VH , and the mouse IgG1 CH sequence amplified from mouse genomic DNA, flanked by AscI and XbaI restriction sites ( ; ).

    Plasmid Preparation:

    Article Title: A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
    Article Snippet: .. The P1316 plasmid was also NotI/AscI digested and gel purified (Qiagen/QiaQuick Gel Extraction Cat# 28706). .. P1316 is a derivative of the pTT3 expression vector ( ) and consists of (5’ to 3’): a CMV promoter, the mouse V kappa leader sequence, a NotI restriction site, an insert consisting of VL /joining fragment/VH , and the mouse IgG1 CH sequence amplified from mouse genomic DNA, flanked by AscI and XbaI restriction sites ( ; ).

    Gel Purification:

    Article Title: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
    Article Snippet: .. T4 polynucleotide kinase (PNK), NotI, NcoI, T4 DNA ligase and accompanying buffers purchased from New England Biolabs 100 mM ATP 100 µM CRISPR target oligonucleotides (designed as in ) and pACYC-GFP Gel purification kit (e.g. Qiagen QIAquick Gel Extraction kit or Promega Wizard SV Gel and PCR Clean-Up System) One Shot TOP10 Competent Cells (Thermo-Fisher) or similar cloning E. coli strain Miniprep kit (Qiagen or Promega) ..

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  • News
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  • Team
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  • 99
    Qiagen qiaquick gel extractin kit
    Qiaquick Gel Extractin Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaquick gel extractin kit/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    qiaquick gel extractin kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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