qiaprep spin miniprep kit  (Qiagen)


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    Structured Review

    Qiagen qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Qiagen
    Average 99 stars, based on 1691 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: For an initial assessment of suitability of plasmid purification kits for separation of the Borrelia main chromosome from plasmids, three different plasmid purification kits were run in duplicate, two from Qiagen and one from Promega (QIAGEN® Plasmid Mini kit (QIAGEN®, cat.nr. .. : 12123); QIAprep® Spin Miniprep kit (QIAGEN®, cat.nr.

    DNA Purification:

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: Paragraph title: DNA purification, plasmid enrichment and library construction ... : 12123); QIAprep® Spin Miniprep kit (QIAGEN®, cat.nr.

    Sequencing:

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: To investigate whether sequence assembly would improve for plasmids if the main linear chromosome was removed prior to library construction, we used plasmid purification kits. .. : 12123); QIAprep® Spin Miniprep kit (QIAGEN®, cat.nr.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: .. : 12123); QIAprep® Spin Miniprep kit (QIAGEN®, cat.nr. .. : 27104); Wizard® Plus SV Minipreps DNA Purification System (Promega, cat.nr.

    Plasmid Preparation:

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: Paragraph title: DNA purification, plasmid enrichment and library construction ... : 12123); QIAprep® Spin Miniprep kit (QIAGEN®, cat.nr.

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  • 95
    Qiagen qiaprep spin miniprep kit
    Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the <t>Qiaprep</t> Spin <t>Miniprep</t> kit for plasmid DNA (0.9% agarose).
    Qiaprep Spin Miniprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Qiagen
    Average 95 stars, based on 1691 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    99
    Qiagen post transfection
    Analysis of NHEJ in MM cells. (A) Map of pEGFP-Pem1-Ad2 modified from reference [ 36 ]. (B) Dot plots of nontransfected LINF903 cells (panel 1), LINF903 cells transfected with 2 μg pDSRed2-N1 (panel 2), with 0.5 μg of pEGFP-Pem1 (panel 3), or with both plasmids together (panel 4). Numbers of green and red cells were determined 24h after <t>transfection</t> by FACS. (C) Dot plots of LINF903 and U266 cell lines transfected with 0.5 μg of pEGFP-Pem1 or 0.5 μg of Hind III-digested pEGFP-Pem1-Ad2 plasmid, together with 2 μg of pDSRed2-N1. Total represented events were adjusted to correct for differences in transfection efficiencies, and same numbers of cells transfected with circular and/or control pDSRed2-N1 are shown (6,000 cells). These numbers of events were then represented in panels corresponding to transfections with digested molecules. (D) Percentage of NHEJ of Hind III - or Sce I -digested plasmid in different cell lines. Mean of a minimum of three independent experiments is shown. (** p
    Post Transfection, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/post transfection/product/Qiagen
    Average 99 stars, based on 132 article reviews
    Price from $9.99 to $1999.99
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    79
    Qiagen 5 hmc test f
    Overview of Tet-assisted bisulfite sequencing (TAB-seq). <t>5-hmC</t> is protected specifically by β-GT to generate 5-gmC, followed by oxidation of 5-mC to 5-caC by mTet1. Only 5-gmC is read as C after bisulfite treatment and PCR amplification.
    5 Hmc Test F, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 hmc test f/product/Qiagen
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the Qiaprep Spin Miniprep kit for plasmid DNA (0.9% agarose).

    Journal: Applied and Environmental Microbiology

    Article Title: Biocidal Efficacy of Copper Alloys against Pathogenic Enterococci Involves Degradation of Genomic and Plasmid DNAs ▿

    doi: 10.1128/AEM.03050-09

    Figure Lengend Snippet: Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the Qiaprep Spin Miniprep kit for plasmid DNA (0.9% agarose).

    Article Snippet: Enterococcal DNA (50-kb fragments) were purified using the Qiagen DNeasy Blood and Tissue kit (following pretreatment with lysozyme), and preparations were separated on a 1, 2, or 3% (wt/vol) agarose gel containing the DNA stain SYBRsafe (Invitrogen) exposed to a current of 300 mA for 90 min. Plasmid DNA was extracted using the QIAprep Spin Miniprep kit (Qiagen), and preparations were separated on 0.9% agarose gels.

    Techniques: Agarose Gel Electrophoresis, Purification, Plasmid Preparation

    DNA sequencing of a site-directed mutagenesis library. DNA was isolated from the library using a QIAGEN QIAprep Spin Miniprep Kit (Valencia, CA), and sequenced using an infrared dye–labeled primer on a LI-COR Model 4200 Automated Sequencer (Lincoln,

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Efficient Site-Directed Saturation Mutagenesis Using Degenerate Oligonucleotides

    doi:

    Figure Lengend Snippet: DNA sequencing of a site-directed mutagenesis library. DNA was isolated from the library using a QIAGEN QIAprep Spin Miniprep Kit (Valencia, CA), and sequenced using an infrared dye–labeled primer on a LI-COR Model 4200 Automated Sequencer (Lincoln,

    Article Snippet: For starting template, we utilized a recombinant plasmid (approximately 6.5 kb) isolated using a QIAGEN QIAprep Spin Miniprep Kit (Valencia, CA).

    Techniques: DNA Sequencing, Mutagenesis, Isolation, Labeling

    Analysis of NHEJ in MM cells. (A) Map of pEGFP-Pem1-Ad2 modified from reference [ 36 ]. (B) Dot plots of nontransfected LINF903 cells (panel 1), LINF903 cells transfected with 2 μg pDSRed2-N1 (panel 2), with 0.5 μg of pEGFP-Pem1 (panel 3), or with both plasmids together (panel 4). Numbers of green and red cells were determined 24h after transfection by FACS. (C) Dot plots of LINF903 and U266 cell lines transfected with 0.5 μg of pEGFP-Pem1 or 0.5 μg of Hind III-digested pEGFP-Pem1-Ad2 plasmid, together with 2 μg of pDSRed2-N1. Total represented events were adjusted to correct for differences in transfection efficiencies, and same numbers of cells transfected with circular and/or control pDSRed2-N1 are shown (6,000 cells). These numbers of events were then represented in panels corresponding to transfections with digested molecules. (D) Percentage of NHEJ of Hind III - or Sce I -digested plasmid in different cell lines. Mean of a minimum of three independent experiments is shown. (** p

    Journal: PLoS ONE

    Article Title: Deregulation of DNA Double-Strand Break Repair in Multiple Myeloma: Implications for Genome Stability

    doi: 10.1371/journal.pone.0121581

    Figure Lengend Snippet: Analysis of NHEJ in MM cells. (A) Map of pEGFP-Pem1-Ad2 modified from reference [ 36 ]. (B) Dot plots of nontransfected LINF903 cells (panel 1), LINF903 cells transfected with 2 μg pDSRed2-N1 (panel 2), with 0.5 μg of pEGFP-Pem1 (panel 3), or with both plasmids together (panel 4). Numbers of green and red cells were determined 24h after transfection by FACS. (C) Dot plots of LINF903 and U266 cell lines transfected with 0.5 μg of pEGFP-Pem1 or 0.5 μg of Hind III-digested pEGFP-Pem1-Ad2 plasmid, together with 2 μg of pDSRed2-N1. Total represented events were adjusted to correct for differences in transfection efficiencies, and same numbers of cells transfected with circular and/or control pDSRed2-N1 are shown (6,000 cells). These numbers of events were then represented in panels corresponding to transfections with digested molecules. (D) Percentage of NHEJ of Hind III - or Sce I -digested plasmid in different cell lines. Mean of a minimum of three independent experiments is shown. (** p

    Article Snippet: Plasmid DNA was extracted from the cells 24h post-transfection [QIAprep spin miniprep kit (Qiagen, Germany)], and transformed into E . coli DH5α cells.

    Techniques: Non-Homologous End Joining, Modification, Transfection, FACS, Plasmid Preparation

    Analysis of HR in normal LINF and MM cell lines. (A) Reporter plasmid for detection of HR [ 22 ]. (B) Cells were transfected with 2 μg of Sce I-digested HR plasmid together with 2 μg of pDSRed2-N1 to normalize for the differences in transfection efficiency. Numbers of green and red cells were determined 48h after transfection by FACS. The ratio of GFP+ cells to DsRed+ cells was used as a measure of repair efficiency. Data are means ± SD of three independent experiments. (C) Representative images showing dot plots corresponding to the indicated cell lines. A total of 6,000 GFP+ and/or DsRed+ cells are shown. (** p

    Journal: PLoS ONE

    Article Title: Deregulation of DNA Double-Strand Break Repair in Multiple Myeloma: Implications for Genome Stability

    doi: 10.1371/journal.pone.0121581

    Figure Lengend Snippet: Analysis of HR in normal LINF and MM cell lines. (A) Reporter plasmid for detection of HR [ 22 ]. (B) Cells were transfected with 2 μg of Sce I-digested HR plasmid together with 2 μg of pDSRed2-N1 to normalize for the differences in transfection efficiency. Numbers of green and red cells were determined 48h after transfection by FACS. The ratio of GFP+ cells to DsRed+ cells was used as a measure of repair efficiency. Data are means ± SD of three independent experiments. (C) Representative images showing dot plots corresponding to the indicated cell lines. A total of 6,000 GFP+ and/or DsRed+ cells are shown. (** p

    Article Snippet: Plasmid DNA was extracted from the cells 24h post-transfection [QIAprep spin miniprep kit (Qiagen, Germany)], and transformed into E . coli DH5α cells.

    Techniques: Plasmid Preparation, Transfection, FACS

    Overview of Tet-assisted bisulfite sequencing (TAB-seq). 5-hmC is protected specifically by β-GT to generate 5-gmC, followed by oxidation of 5-mC to 5-caC by mTet1. Only 5-gmC is read as C after bisulfite treatment and PCR amplification.

    Journal: Nature protocols

    Article Title: Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine

    doi: 10.1038/nprot.2012.137

    Figure Lengend Snippet: Overview of Tet-assisted bisulfite sequencing (TAB-seq). 5-hmC is protected specifically by β-GT to generate 5-gmC, followed by oxidation of 5-mC to 5-caC by mTet1. Only 5-gmC is read as C after bisulfite treatment and PCR amplification.

    Article Snippet: NaCl (Fisher BioReagents, cat. no. BP358-10; dissolved in H2 O at 2 M) l -Ascorbic acid (Sigma-Aldrich, cat. no. 255564; dissolved in H2 O at 40 mM) DTT (Roche Diagnostics, cat. no. 93889320; dissolved in H2 O at 50 mM) Disodium salt ATP (Fisher BioReagents, cat. no. BP413-25; dissolved in H2 O at 24 mM) α-Ketoglutaric acid disodium salt hydrate (α-KG; Sigma-Aldrich, cat. no. K3752; dissolved in H2 O at 20 mM) Tet oxidation reagent 1 (Reagent Setup) Tet oxidation reagent 2 (Reagent Setup) Micro Bio-Spin 30 columns (Bio-Rad, cat. no. 7326203) Proteinase K (Fermentas, cat. no. EO0491) QIAquick gel extraction kit (Qiagen, cat. no. 28704) QIAquick nucleotide removal kit (Qiagen, cat. no. 28304) 5-mC_test_F, 5-mC_test_R, 5-hmC_test_F, 5-hmC_test_F (synthesized by Operon; ) QIAprep spin miniprep kit (Qiagen, cat. no. 27104) Zero Blunt TOPO PCR cloning kit (Invitrogen, cat. no. K2800-20) End-It DNA end-repair kit (Epicentre, cat. no. ER81050).

    Techniques: Methylation Sequencing, Polymerase Chain Reaction, Amplification