qiaprep kit  (Qiagen)

 
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    Name:
    QIAprep 96 Plus BioRobot Kit
    Description:
    For purification of up to 50 μg high quality plasmid DNA in 96 well format Kit contents Qiagen 96 Plus BioRobot Kit 4 x 96 preps 1 25 to 5mL Culture Volume Nonchaotropic Binding Chemistry Technology 40 min Time Run 48 to 96 Samples Run 15 to 50g Yield Automated Processing Preparation of Short Hairpin Vectors For Purification of up to 50μg High quality Plasmid DNA in 96 well Format Includes TurboFilter 96 Plates Plasmid Plus 96 Plates Buffers Reagents Flat bottom Blocks S Blocks and Elution Microtubes Benefits Consistently high yields of up to 50 μg Pure preps suitable for a wide range of applications Vacuum centrifugation and automated processing option
    Catalog Number:
    962241
    Price:
    1302
    Category:
    QIAprep 96 Plus Kits
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    Structured Review

    Qiagen qiaprep kit
    QIAprep 96 Plus BioRobot Kit
    For purification of up to 50 μg high quality plasmid DNA in 96 well format Kit contents Qiagen 96 Plus BioRobot Kit 4 x 96 preps 1 25 to 5mL Culture Volume Nonchaotropic Binding Chemistry Technology 40 min Time Run 48 to 96 Samples Run 15 to 50g Yield Automated Processing Preparation of Short Hairpin Vectors For Purification of up to 50μg High quality Plasmid DNA in 96 well Format Includes TurboFilter 96 Plates Plasmid Plus 96 Plates Buffers Reagents Flat bottom Blocks S Blocks and Elution Microtubes Benefits Consistently high yields of up to 50 μg Pure preps suitable for a wide range of applications Vacuum centrifugation and automated processing option
    https://www.bioz.com/result/qiaprep kit/product/Qiagen
    Average 95 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    qiaprep kit - by Bioz Stars, 2020-08
    95/100 stars

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    Sequencing:

    Article Title: Design, construction, and validation of a modular library of sequence diversity standards for PCR
    Article Snippet: .. Transformants were cultured, prepped with the Qiaprep 96 kit (Qiagen), and used for Sanger sequencing. ..

    Clone Assay:

    Article Title: TonB induces conformational changes in surface-exposed loops of FhuA, outer membrane receptor of Escherichia coli
    Article Snippet: .. From clones selected after the third round of panning, phage DNA was purified using QIAprep 96 M13 Kit (Qiagen). .. If sequence diversity after three rounds of panning was apparently low, phage clones were selected from round 2 eluates.

    Cell Culture:

    Article Title: Design, construction, and validation of a modular library of sequence diversity standards for PCR
    Article Snippet: .. Transformants were cultured, prepped with the Qiaprep 96 kit (Qiagen), and used for Sanger sequencing. ..

    Purification:

    Article Title: TonB induces conformational changes in surface-exposed loops of FhuA, outer membrane receptor of Escherichia coli
    Article Snippet: .. From clones selected after the third round of panning, phage DNA was purified using QIAprep 96 M13 Kit (Qiagen). .. If sequence diversity after three rounds of panning was apparently low, phage clones were selected from round 2 eluates.

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  • 99
    Qiagen post transfection
    Analysis of NHEJ in MM cells. (A) Map of pEGFP-Pem1-Ad2 modified from reference [ 36 ]. (B) Dot plots of nontransfected LINF903 cells (panel 1), LINF903 cells transfected with 2 μg pDSRed2-N1 (panel 2), with 0.5 μg of pEGFP-Pem1 (panel 3), or with both plasmids together (panel 4). Numbers of green and red cells were determined 24h after <t>transfection</t> by FACS. (C) Dot plots of LINF903 and U266 cell lines transfected with 0.5 μg of pEGFP-Pem1 or 0.5 μg of Hind III-digested pEGFP-Pem1-Ad2 plasmid, together with 2 μg of pDSRed2-N1. Total represented events were adjusted to correct for differences in transfection efficiencies, and same numbers of cells transfected with circular and/or control pDSRed2-N1 are shown (6,000 cells). These numbers of events were then represented in panels corresponding to transfections with digested molecules. (D) Percentage of NHEJ of Hind III - or Sce I -digested plasmid in different cell lines. Mean of a minimum of three independent experiments is shown. (** p
    Post Transfection, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/post transfection/product/Qiagen
    Average 99 stars, based on 2287 article reviews
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    94
    Qiagen 96 well deep well plates
    Screening for cellular modulators of A4V SOD1: The enriched pools (1584) or controls were transfected with A4V SOD1 reporter system in <t>96-well</t> plates. The fluorescence signal was measured 48 h after transfection after the addition of the substrate. (
    96 Well Deep Well Plates, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well deep well plates/product/Qiagen
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    96 well deep well plates - by Bioz Stars, 2020-08
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    Image Search Results


    Analysis of NHEJ in MM cells. (A) Map of pEGFP-Pem1-Ad2 modified from reference [ 36 ]. (B) Dot plots of nontransfected LINF903 cells (panel 1), LINF903 cells transfected with 2 μg pDSRed2-N1 (panel 2), with 0.5 μg of pEGFP-Pem1 (panel 3), or with both plasmids together (panel 4). Numbers of green and red cells were determined 24h after transfection by FACS. (C) Dot plots of LINF903 and U266 cell lines transfected with 0.5 μg of pEGFP-Pem1 or 0.5 μg of Hind III-digested pEGFP-Pem1-Ad2 plasmid, together with 2 μg of pDSRed2-N1. Total represented events were adjusted to correct for differences in transfection efficiencies, and same numbers of cells transfected with circular and/or control pDSRed2-N1 are shown (6,000 cells). These numbers of events were then represented in panels corresponding to transfections with digested molecules. (D) Percentage of NHEJ of Hind III - or Sce I -digested plasmid in different cell lines. Mean of a minimum of three independent experiments is shown. (** p

    Journal: PLoS ONE

    Article Title: Deregulation of DNA Double-Strand Break Repair in Multiple Myeloma: Implications for Genome Stability

    doi: 10.1371/journal.pone.0121581

    Figure Lengend Snippet: Analysis of NHEJ in MM cells. (A) Map of pEGFP-Pem1-Ad2 modified from reference [ 36 ]. (B) Dot plots of nontransfected LINF903 cells (panel 1), LINF903 cells transfected with 2 μg pDSRed2-N1 (panel 2), with 0.5 μg of pEGFP-Pem1 (panel 3), or with both plasmids together (panel 4). Numbers of green and red cells were determined 24h after transfection by FACS. (C) Dot plots of LINF903 and U266 cell lines transfected with 0.5 μg of pEGFP-Pem1 or 0.5 μg of Hind III-digested pEGFP-Pem1-Ad2 plasmid, together with 2 μg of pDSRed2-N1. Total represented events were adjusted to correct for differences in transfection efficiencies, and same numbers of cells transfected with circular and/or control pDSRed2-N1 are shown (6,000 cells). These numbers of events were then represented in panels corresponding to transfections with digested molecules. (D) Percentage of NHEJ of Hind III - or Sce I -digested plasmid in different cell lines. Mean of a minimum of three independent experiments is shown. (** p

    Article Snippet: Plasmid DNA was extracted from the cells 24h post-transfection [QIAprep spin miniprep kit (Qiagen, Germany)], and transformed into E . coli DH5α cells.

    Techniques: Non-Homologous End Joining, Modification, Transfection, FACS, Plasmid Preparation

    Analysis of HR in normal LINF and MM cell lines. (A) Reporter plasmid for detection of HR [ 22 ]. (B) Cells were transfected with 2 μg of Sce I-digested HR plasmid together with 2 μg of pDSRed2-N1 to normalize for the differences in transfection efficiency. Numbers of green and red cells were determined 48h after transfection by FACS. The ratio of GFP+ cells to DsRed+ cells was used as a measure of repair efficiency. Data are means ± SD of three independent experiments. (C) Representative images showing dot plots corresponding to the indicated cell lines. A total of 6,000 GFP+ and/or DsRed+ cells are shown. (** p

    Journal: PLoS ONE

    Article Title: Deregulation of DNA Double-Strand Break Repair in Multiple Myeloma: Implications for Genome Stability

    doi: 10.1371/journal.pone.0121581

    Figure Lengend Snippet: Analysis of HR in normal LINF and MM cell lines. (A) Reporter plasmid for detection of HR [ 22 ]. (B) Cells were transfected with 2 μg of Sce I-digested HR plasmid together with 2 μg of pDSRed2-N1 to normalize for the differences in transfection efficiency. Numbers of green and red cells were determined 48h after transfection by FACS. The ratio of GFP+ cells to DsRed+ cells was used as a measure of repair efficiency. Data are means ± SD of three independent experiments. (C) Representative images showing dot plots corresponding to the indicated cell lines. A total of 6,000 GFP+ and/or DsRed+ cells are shown. (** p

    Article Snippet: Plasmid DNA was extracted from the cells 24h post-transfection [QIAprep spin miniprep kit (Qiagen, Germany)], and transformed into E . coli DH5α cells.

    Techniques: Plasmid Preparation, Transfection, FACS

    Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the Qiaprep Spin Miniprep kit for plasmid DNA (0.9% agarose).

    Journal: Applied and Environmental Microbiology

    Article Title: Biocidal Efficacy of Copper Alloys against Pathogenic Enterococci Involves Degradation of Genomic and Plasmid DNAs ▿

    doi: 10.1128/AEM.03050-09

    Figure Lengend Snippet: Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the Qiaprep Spin Miniprep kit for plasmid DNA (0.9% agarose).

    Article Snippet: Enterococcal DNA (50-kb fragments) were purified using the Qiagen DNeasy Blood and Tissue kit (following pretreatment with lysozyme), and preparations were separated on a 1, 2, or 3% (wt/vol) agarose gel containing the DNA stain SYBRsafe (Invitrogen) exposed to a current of 300 mA for 90 min. Plasmid DNA was extracted using the QIAprep Spin Miniprep kit (Qiagen), and preparations were separated on 0.9% agarose gels.

    Techniques: Agarose Gel Electrophoresis, Purification, Plasmid Preparation

    Overview of Tet-assisted bisulfite sequencing (TAB-seq). 5-hmC is protected specifically by β-GT to generate 5-gmC, followed by oxidation of 5-mC to 5-caC by mTet1. Only 5-gmC is read as C after bisulfite treatment and PCR amplification.

    Journal: Nature protocols

    Article Title: Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine

    doi: 10.1038/nprot.2012.137

    Figure Lengend Snippet: Overview of Tet-assisted bisulfite sequencing (TAB-seq). 5-hmC is protected specifically by β-GT to generate 5-gmC, followed by oxidation of 5-mC to 5-caC by mTet1. Only 5-gmC is read as C after bisulfite treatment and PCR amplification.

    Article Snippet: NaCl (Fisher BioReagents, cat. no. BP358-10; dissolved in H2 O at 2 M) l -Ascorbic acid (Sigma-Aldrich, cat. no. 255564; dissolved in H2 O at 40 mM) DTT (Roche Diagnostics, cat. no. 93889320; dissolved in H2 O at 50 mM) Disodium salt ATP (Fisher BioReagents, cat. no. BP413-25; dissolved in H2 O at 24 mM) α-Ketoglutaric acid disodium salt hydrate (α-KG; Sigma-Aldrich, cat. no. K3752; dissolved in H2 O at 20 mM) Tet oxidation reagent 1 (Reagent Setup) Tet oxidation reagent 2 (Reagent Setup) Micro Bio-Spin 30 columns (Bio-Rad, cat. no. 7326203) Proteinase K (Fermentas, cat. no. EO0491) QIAquick gel extraction kit (Qiagen, cat. no. 28704) QIAquick nucleotide removal kit (Qiagen, cat. no. 28304) 5-mC_test_F, 5-mC_test_R, 5-hmC_test_F, 5-hmC_test_F (synthesized by Operon; ) QIAprep spin miniprep kit (Qiagen, cat. no. 27104) Zero Blunt TOPO PCR cloning kit (Invitrogen, cat. no. K2800-20) End-It DNA end-repair kit (Epicentre, cat. no. ER81050).

    Techniques: Methylation Sequencing, Polymerase Chain Reaction, Amplification

    Screening for cellular modulators of A4V SOD1: The enriched pools (1584) or controls were transfected with A4V SOD1 reporter system in 96-well plates. The fluorescence signal was measured 48 h after transfection after the addition of the substrate. (

    Journal: Journal of biomolecular screening

    Article Title: A Screen to Identify cellular Modulators of Soluble Levels of an Amyotrophic Lateral Sclerosis (ALS)-causing Mutant SOD1

    doi: 10.1177/1087057111418505

    Figure Lengend Snippet: Screening for cellular modulators of A4V SOD1: The enriched pools (1584) or controls were transfected with A4V SOD1 reporter system in 96-well plates. The fluorescence signal was measured 48 h after transfection after the addition of the substrate. (

    Article Snippet: The cells were grown in 1.25 mL/well TB-Amp medium in 96-well deep-well plates (QIAPrep 96 Turbo Miniprep Kit; QIAGEN, Valencia, CA).

    Techniques: Transfection, Fluorescence