qiaprep kit  (Qiagen)


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    Structured Review

    Qiagen qiaprep kit
    Qiaprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep kit/product/Qiagen
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    qiaprep kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Methylation Sequencing:

    Article Title: Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines
    Article Snippet: Paragraph title: Cloning and bisulfite sequencing of the CpG Island of the MMP2 gene ... Plasmid DNA was extracted from at least eight clones using the QiaPrep kit (Qiagen).

    Clone Assay:

    Article Title: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors
    Article Snippet: Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France). .. Pfu DNA Polymerase (Roche) was used to generate PCR products for cloning, and Taq Polymerase (New England BioLabs) was used for screening colonies by PCR with standard PCR protocols employed for all reactions.

    Article Title: Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines
    Article Snippet: .. Plasmid DNA was extracted from at least eight clones using the QiaPrep kit (Qiagen). .. Approximately 300 to 500 ng of DNA were sequenced using the BigDye terminator kit on an XL Genetic Analyzer sequencer according to the manufacturer’s instructions (Applied Biosystems).

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: Construction of hemidriver lines Lines were constructed essentially as described by using entry clones generated as described in . .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: Paragraph title: Cloning and domain-swapping ... Plasmids were extracted and purified with the Qiaprep kit (Qiagen).

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: Lines were constructed essentially as described by using entry clones generated as described in . .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
    Article Snippet: Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France). .. Pfu DNA Polymerase (Roche) was used to generate PCR products for cloning, and Taq Polymerase (New England BioLabs) was used for screening colonies by PCR with standard PCR protocols employed for all reactions.

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: Paragraph title: Cloning and Sequencing ... Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Article Title: Diverse Antibiotic Resistance Genes in Dairy Cow Manure
    Article Snippet: To better understand the genomic context of twelve distinct clones conferring AR, we sequenced the entire fosmid in each using Pacific Biosciences (PacBio) RS technology. .. DNA was prepared using the QIAprep kit (Qiagen) from 10 ml of bacterial culture in LB supplemented with chloramphenicol and 1× CopyControl induction solution (Epicentre).

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: Intron mobility was evaluated by PCR amplification of the target sites using an Escherichia coli two-plasmid assay in which the studied intron sequences had previously been cloned into a pUC18-derived vector and which is aimed to target a specific site cloned into pACYC184 ( ). .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France).

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector. .. By qPCR we analyzed the level of expression of the major variants of NKX2-1 mRNA.

    Amplification:

    Article Title: Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines
    Article Snippet: The CpG island of the MMP2 gene was amplified using the primers described previously , and the products were purified by 1% agarose gel electrophoresis using the Quick Extraction Protocols Kit (Qiagen). .. Plasmid DNA was extracted from at least eight clones using the QiaPrep kit (Qiagen).

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: Plasmids were extracted and purified with the Qiaprep kit (Qiagen). .. To replace a selected stretch of the photopigment sequence with that of another, a plasmid containing the donor sequence served as template; primers incorporating suitable unique restriction sites in their 5' end (Bmt-I and Age-I ) were used in a touch-up PCR protocol (necessary because of the presence of 12 non-complementary bases in the primers), and the resulting product was ligated into a pGemT-easy vector and amplified in transformed bacteria, followed by plasmid extraction and purification; the identity of the insert was corroborated at various stages by colony PCR, restriction analysis, and sequencing.

    Article Title: Genomics of Clostridium taeniosporum, an organism which forms endospores with ribbon-like appendages
    Article Snippet: Plasmids were purified by a QIAprep Kit [QIAGEN Inc., Valencia, CA 91355]. .. Reagents included Taq DNA polymerase from Roche (Branford, CT), deoxyribonucleotides from Sigma-Aldrich (St Louis, MO) for short sequences and the RangerMix from Bioline (Taunton, MA) for amplification of fragments longer than 6 kbp.

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: To confirm that the DNA amplification in both PCR and qPCR reactions were products of H. glycines DNA and not spurious amplification of off-target DNA, DNA amplification products were run out electrophoretically on and then isolated from the 1% agarose gels. .. Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: Intron mobility was evaluated by PCR amplification of the target sites using an Escherichia coli two-plasmid assay in which the studied intron sequences had previously been cloned into a pUC18-derived vector and which is aimed to target a specific site cloned into pACYC184 ( ). .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France).

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: To reduce the amplification of unwanted products during the sequential nested PCR with the specific NKX2-1-AS1 nested primer NGSP1 (Supplementary Table ), the annealing temperature was increased to 70 °C and the number of cycles to 30. .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector.

    Construct:

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: BPp65ADZp and BPZpGDBD, hemidriver constructs that lack an enhancer fragment, were constructed by substituting the GAL4 coding sequence in pBPGAL4U ( ) with the split-GAL4 coding sequences from pBPp65ADZpUw and pBPZpGAL4DBDUw, respectively, using Kpn I and Hin dIII sites. .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: BPp65ADZp and BPZpGDBD, hemidriver constructs that lack an enhancer fragment, were constructed by substituting the GAL4 coding sequence in pBPGAL4U ( ) with the split-GAL4 coding sequences from pBPp65ADZpUw and pBPZpGAL4DBDUw, respectively, using Kpn I and Hin dIII sites. .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: NOVEL STAT1 MUTATION DISRUPTS SMALL UBIQUITIN-RELATED MODIFIER (SUMO) CONJUGATION CAUSING GAIN OF FUNCTION
    Article Snippet: Paragraph title: Constructs ... All plasmids were isolated using the QIAprep kit (QIAGEN) according to manufacturer’s recommendations and mutations were verified by sequencing.

    Real-time Polymerase Chain Reaction:

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: To confirm that the DNA amplification in both PCR and qPCR reactions were products of H. glycines DNA and not spurious amplification of off-target DNA, DNA amplification products were run out electrophoretically on and then isolated from the 1% agarose gels. .. Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Incubation:

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France). .. Mobility was evaluated using pACYC-EcoRI-A or pACYC-EcoRI-B and intron-specific primers ( ).

    Expressing:

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France). .. Mobility was evaluated using pACYC-EcoRI-A or pACYC-EcoRI-B and intron-specific primers ( ).

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: In the rapid amplification step with the NKX2-1-AS1 specific primer GSP1 (Supplementary Table ) we increased the number of cycles to 30 to improve amplification of transcripts with low expression. .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector.

    Article Title: NOVEL STAT1 MUTATION DISRUPTS SMALL UBIQUITIN-RELATED MODIFIER (SUMO) CONJUGATION CAUSING GAIN OF FUNCTION
    Article Snippet: Mutated STAT1 sequences and GFP-tagged constructs were created (BioInnovatise Inc., Rockville, MD) using a STAT1 expression vector (Origene Technologies, Rockville, MD). pSG5-His-SUMO1 and Flag-SENP1 constructs were obtained from Addgene (Cambridge, MA) ( , ). .. All plasmids were isolated using the QIAprep kit (QIAGEN) according to manufacturer’s recommendations and mutations were verified by sequencing.

    Transformation Assay:

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: Plasmids were extracted and purified with the Qiaprep kit (Qiagen). .. To replace a selected stretch of the photopigment sequence with that of another, a plasmid containing the donor sequence served as template; primers incorporating suitable unique restriction sites in their 5' end (Bmt-I and Age-I ) were used in a touch-up PCR protocol (necessary because of the presence of 12 non-complementary bases in the primers), and the resulting product was ligated into a pGemT-easy vector and amplified in transformed bacteria, followed by plasmid extraction and purification; the identity of the insert was corroborated at various stages by colony PCR, restriction analysis, and sequencing.

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: For each mobility assay, the intron donor plasmid (Kmr ) and the recipient plasmid (Tcr and Cms ) were simultaneously transformed into E. coli TOP10 competent cells and subjected to Km and Tc selection. .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France).

    Hybridization:

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France). .. GOC) intron was determined by colony replicate hybridization or directly by PCR.

    Ligation:

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: The ligation reaction was shuttled into competent JM109 E. coli cells and selected on 50 µg/ml ampicillin on LB-agar plates. .. Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Cell Culture:

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: For Sanger sequencing, products were ligated into pGEM-T-Easy (Promega) used to tra nsform JM109 bacteria (Promega), which were then cultured in X-Gal/IPTG agar dishes, following standard procedures. .. Plasmids were extracted and purified with the Qiaprep kit (Qiagen).

    Generated:

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: Construction of hemidriver lines Lines were constructed essentially as described by using entry clones generated as described in . .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: Lines were constructed essentially as described by using entry clones generated as described in . .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Diverse Antibiotic Resistance Genes in Dairy Cow Manure
    Article Snippet: DNA was prepared using the QIAprep kit (Qiagen) from 10 ml of bacterial culture in LB supplemented with chloramphenicol and 1× CopyControl induction solution (Epicentre). .. The resulting scaffolds were assigned to the fosmids by performing a BLASTN search for the resistance genes and end sequences generated by Sanger sequencing of the fosmid vector using the primers recommended by the manufacturer.

    Polymerase Chain Reaction:

    Article Title: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors
    Article Snippet: Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France). .. Pfu DNA Polymerase (Roche) was used to generate PCR products for cloning, and Taq Polymerase (New England BioLabs) was used for screening colonies by PCR with standard PCR protocols employed for all reactions.

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: Cloning and domain-swapping RACE-ready cDNA from Pecten retinae was prepared using the Clontech SMART RACE kit; PCR amplifications and RACE extensions were conducted as previously described [ ]. .. Plasmids were extracted and purified with the Qiaprep kit (Qiagen).

    Article Title: Genomics of Clostridium taeniosporum, an organism which forms endospores with ribbon-like appendages
    Article Snippet: Plasmids were purified by a QIAprep Kit [QIAGEN Inc., Valencia, CA 91355]. .. PCR reactions were conducted in a MasterCycler Personal (Eppendorf AG, Hamburg, Germany).

    Article Title: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
    Article Snippet: Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France). .. Pfu DNA Polymerase (Roche) was used to generate PCR products for cloning, and Taq Polymerase (New England BioLabs) was used for screening colonies by PCR with standard PCR protocols employed for all reactions.

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: To confirm that the DNA amplification in both PCR and qPCR reactions were products of H. glycines DNA and not spurious amplification of off-target DNA, DNA amplification products were run out electrophoretically on and then isolated from the 1% agarose gels. .. Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: Intron mobility was evaluated by PCR amplification of the target sites using an Escherichia coli two-plasmid assay in which the studied intron sequences had previously been cloned into a pUC18-derived vector and which is aimed to target a specific site cloned into pACYC184 ( ). .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France).

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: PCR products were separated by gel electrophoresis and the fragments purified using a gel extraction kit (Qiagen). .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector.

    Recombinant:

    Article Title: Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines
    Article Snippet: Recombinant clones were selected by α-complementation. .. Plasmid DNA was extracted from at least eight clones using the QiaPrep kit (Qiagen).

    Nucleic Acid Electrophoresis:

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: PCR products were separated by gel electrophoresis and the fragments purified using a gel extraction kit (Qiagen). .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector.

    Isolation:

    Article Title: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors
    Article Snippet: DNA manipulation techniques Total genomic DNA from C. acetobutylicum and C. saccharobutylicum were isolated as previously described [ ]. .. Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France).

    Article Title: Giardia Duodenalis 14-3-3 Protein Is Polyglycylated by a Tubulin Tyrosine Ligase-like Member and Deglycylated by Two Metallocarboxypeptidases *
    Article Snippet: .. Plasmid DNA was isolated from bacteria using the QIAPrep kit (Qiagen). .. Escherichia coli JM109 competent cells were used for vector manipulation and propagation.

    Article Title: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
    Article Snippet: DNA manipulation techniques Total genomic DNA from C. acetobutylicum was isolated as previously described [ ]. .. Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France).

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: .. Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen). ..

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector. .. By qPCR we analyzed the level of expression of the major variants of NKX2-1 mRNA.

    Article Title: NOVEL STAT1 MUTATION DISRUPTS SMALL UBIQUITIN-RELATED MODIFIER (SUMO) CONJUGATION CAUSING GAIN OF FUNCTION
    Article Snippet: .. All plasmids were isolated using the QIAprep kit (QIAGEN) according to manufacturer’s recommendations and mutations were verified by sequencing. .. Tyrosine (Tyr701, Y701) STAT1 phosphorylation (pSTAT1) was assayed in transfected cells (WT, STAT1 mutants and SENP-1 constructs) stimulated with IFN-γ (1,000 IU/ml).

    Purification:

    Article Title: Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines
    Article Snippet: The purified products were then cloned into the pGEMT Easy vector (Promega) and electroporated into the Escherichia coli strain DH10B. .. Plasmid DNA was extracted from at least eight clones using the QiaPrep kit (Qiagen).

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: .. Plasmids were extracted and purified with the Qiaprep kit (Qiagen). .. To replace a selected stretch of the photopigment sequence with that of another, a plasmid containing the donor sequence served as template; primers incorporating suitable unique restriction sites in their 5' end (Bmt-I and Age-I ) were used in a touch-up PCR protocol (necessary because of the presence of 12 non-complementary bases in the primers), and the resulting product was ligated into a pGemT-easy vector and amplified in transformed bacteria, followed by plasmid extraction and purification; the identity of the insert was corroborated at various stages by colony PCR, restriction analysis, and sequencing.

    Article Title: Genomics of Clostridium taeniosporum, an organism which forms endospores with ribbon-like appendages
    Article Snippet: .. Plasmids were purified by a QIAprep Kit [QIAGEN Inc., Valencia, CA 91355]. .. PCR reactions were conducted in a MasterCycler Personal (Eppendorf AG, Hamburg, Germany).

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: The DNA was purified using the Qiaquick Gel Extraction Kit (Qiagen; Valencia, CA) as according to the manufacturer’s specifications. .. Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector. .. By qPCR we analyzed the level of expression of the major variants of NKX2-1 mRNA.

    Sequencing:

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: BPp65ADZp and BPZpGDBD, hemidriver constructs that lack an enhancer fragment, were constructed by substituting the GAL4 coding sequence in pBPGAL4U ( ) with the split-GAL4 coding sequences from pBPp65ADZpUw and pBPZpGAL4DBDUw, respectively, using Kpn I and Hin dIII sites. .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: For Sanger sequencing, products were ligated into pGEM-T-Easy (Promega) used to tra nsform JM109 bacteria (Promega), which were then cultured in X-Gal/IPTG agar dishes, following standard procedures. .. Plasmids were extracted and purified with the Qiaprep kit (Qiagen).

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: BPp65ADZp and BPZpGDBD, hemidriver constructs that lack an enhancer fragment, were constructed by substituting the GAL4 coding sequence in pBPGAL4U ( ) with the split-GAL4 coding sequences from pBPp65ADZpUw and pBPZpGAL4DBDUw, respectively, using Kpn I and Hin dIII sites. .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: Paragraph title: Cloning and Sequencing ... Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Article Title: Diverse Antibiotic Resistance Genes in Dairy Cow Manure
    Article Snippet: Paragraph title: Sequencing of fosmids carrying AR genes. ... DNA was prepared using the QIAprep kit (Qiagen) from 10 ml of bacterial culture in LB supplemented with chloramphenicol and 1× CopyControl induction solution (Epicentre).

    Article Title: NOVEL STAT1 MUTATION DISRUPTS SMALL UBIQUITIN-RELATED MODIFIER (SUMO) CONJUGATION CAUSING GAIN OF FUNCTION
    Article Snippet: .. All plasmids were isolated using the QIAprep kit (QIAGEN) according to manufacturer’s recommendations and mutations were verified by sequencing. .. Tyrosine (Tyr701, Y701) STAT1 phosphorylation (pSTAT1) was assayed in transfected cells (WT, STAT1 mutants and SENP-1 constructs) stimulated with IFN-γ (1,000 IU/ml).

    Nested PCR:

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: To reduce the amplification of unwanted products during the sequential nested PCR with the specific NKX2-1-AS1 nested primer NGSP1 (Supplementary Table ), the annealing temperature was increased to 70 °C and the number of cycles to 30. .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector.

    Plasmid Preparation:

    Article Title: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors
    Article Snippet: .. Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France). .. Pfu DNA Polymerase (Roche) was used to generate PCR products for cloning, and Taq Polymerase (New England BioLabs) was used for screening colonies by PCR with standard PCR protocols employed for all reactions.

    Article Title: Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines
    Article Snippet: .. Plasmid DNA was extracted from at least eight clones using the QiaPrep kit (Qiagen). .. Approximately 300 to 500 ng of DNA were sequenced using the BigDye terminator kit on an XL Genetic Analyzer sequencer according to the manufacturer’s instructions (Applied Biosystems).

    Article Title: Giardia Duodenalis 14-3-3 Protein Is Polyglycylated by a Tubulin Tyrosine Ligase-like Member and Deglycylated by Two Metallocarboxypeptidases *
    Article Snippet: .. Plasmid DNA was isolated from bacteria using the QIAPrep kit (Qiagen). .. Escherichia coli JM109 competent cells were used for vector manipulation and propagation.

    Article Title: Light control of G protein signaling pathways by a novel photopigment
    Article Snippet: Plasmids were extracted and purified with the Qiaprep kit (Qiagen). .. To replace a selected stretch of the photopigment sequence with that of another, a plasmid containing the donor sequence served as template; primers incorporating suitable unique restriction sites in their 5' end (Bmt-I and Age-I ) were used in a touch-up PCR protocol (necessary because of the presence of 12 non-complementary bases in the primers), and the resulting product was ligated into a pGemT-easy vector and amplified in transformed bacteria, followed by plasmid extraction and purification; the identity of the insert was corroborated at various stages by colony PCR, restriction analysis, and sequencing.

    Article Title: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
    Article Snippet: .. Plasmid DNA was extracted from E. coli with the QIAprep kit (Qiagen, France). .. Pfu DNA Polymerase (Roche) was used to generate PCR products for cloning, and Taq Polymerase (New England BioLabs) was used for screening colonies by PCR with standard PCR protocols employed for all reactions.

    Article Title: Diverse Antibiotic Resistance Genes in Dairy Cow Manure
    Article Snippet: DNA was prepared using the QIAprep kit (Qiagen) from 10 ml of bacterial culture in LB supplemented with chloramphenicol and 1× CopyControl induction solution (Epicentre). .. Assembly was performed using Celera ( ) and trimmed for fosmid vector sequence using the ContaminationTrimmer.py command ( https://github.com/PacificBiosciences/HBAR-DTK) .

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France). .. Mobility was evaluated using pACYC-EcoRI-A or pACYC-EcoRI-B and intron-specific primers ( ).

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector. .. By qPCR we analyzed the level of expression of the major variants of NKX2-1 mRNA.

    Article Title: NOVEL STAT1 MUTATION DISRUPTS SMALL UBIQUITIN-RELATED MODIFIER (SUMO) CONJUGATION CAUSING GAIN OF FUNCTION
    Article Snippet: Mutated STAT1 sequences and GFP-tagged constructs were created (BioInnovatise Inc., Rockville, MD) using a STAT1 expression vector (Origene Technologies, Rockville, MD). pSG5-His-SUMO1 and Flag-SENP1 constructs were obtained from Addgene (Cambridge, MA) ( , ). .. All plasmids were isolated using the QIAprep kit (QIAGEN) according to manufacturer’s recommendations and mutations were verified by sequencing.

    Selection:

    Article Title: Group IIC Intron with an Unusual Target of Integration in Enterobacter cloacae
    Article Snippet: For each mobility assay, the intron donor plasmid (Kmr ) and the recipient plasmid (Tcr and Cms ) were simultaneously transformed into E. coli TOP10 competent cells and subjected to Km and Tc selection. .. Expression of the intron was then induced by addition of 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG), followed by incubation at 37°C for 3 h. Plasmid DNAs were extracted using a QIAprep kit (Qiagen, Courtaboeuf, France).

    Agarose Gel Electrophoresis:

    Article Title: Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines
    Article Snippet: The CpG island of the MMP2 gene was amplified using the primers described previously , and the products were purified by 1% agarose gel electrophoresis using the Quick Extraction Protocols Kit (Qiagen). .. Plasmid DNA was extracted from at least eight clones using the QiaPrep kit (Qiagen).

    Activation Assay:

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: The p65 AD replaces the native GAL4 AD in pBPp65ADZpUw, which results in stronger transcriptional activation and insensitivity to inactivation by GAL80. .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    Article Title: Genetic Reagents for Making Split-GAL4 Lines in Drosophila
    Article Snippet: The p65 AD replaces the native GAL4 AD in pBPp65ADZpUw, which results in stronger transcriptional activation and insensitivity to inactivation by GAL80. .. DNA for injections was prepared from 5-ml overnight cultures using a QIAprep kit (QIAGEN, Valencia, CA) and verified by Eco RI restriction digestion.

    DNA Purification:

    Article Title: Genomics of Clostridium taeniosporum, an organism which forms endospores with ribbon-like appendages
    Article Snippet: Chromosomal DNA was extracted from cells growing exponentially by the Puregene Genomic DNA Purification kit (Gentra Systems, Minneapolis, MN). .. Plasmids were purified by a QIAprep Kit [QIAGEN Inc., Valencia, CA 91355].

    High Throughput Screening Assay:

    Article Title: Diverse Antibiotic Resistance Genes in Dairy Cow Manure
    Article Snippet: DNA was prepared using the QIAprep kit (Qiagen) from 10 ml of bacterial culture in LB supplemented with chloramphenicol and 1× CopyControl induction solution (Epicentre). .. From each fosmid clone, 300 ng of DNA was pooled into a single reaction mixture and submitted to the Yale high-throughput sequencing center ( http://medicine.yale.edu/keck/ycga ).

    Gel Extraction:

    Article Title: Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
    Article Snippet: The DNA was purified using the Qiaquick Gel Extraction Kit (Qiagen; Valencia, CA) as according to the manufacturer’s specifications. .. Plasmid DNA was isolated from the bacteria using the Qiaprep kit (Qiagen).

    Article Title: NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration
    Article Snippet: PCR products were separated by gel electrophoresis and the fragments purified using a gel extraction kit (Qiagen). .. DNA from isolated clones was purified using the QIAprep kit (Qiagen) and sequenced using NGSP1 primer, M13F, and M13R primers from the vector.

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    Qiagen qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 3423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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