qiamp dna blood mini kit  (Qiagen)

 
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    Name:
    QIAamp DNA Blood Mini Kit
    Description:
    For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
    Catalog Number:
    51104
    Price:
    157
    Category:
    QIAamp DNA Blood Mini Kit
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    Structured Review

    Qiagen qiamp dna blood mini kit
    QIAamp DNA Blood Mini Kit
    For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
    https://www.bioz.com/result/qiamp dna blood mini kit/product/Qiagen
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    qiamp dna blood mini kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood
    Article Snippet: .. The white blood cell pellets were resuspended in 200 μ l PBS and subsequently spiked with 4·4 × 103 CFU, 4·4 × 104 CFU, 4·4 × 105 CFU or 4·4 × 106 CFU of CVD 909 and DNA extraction was performed using the QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer's instructions. .. The purified DNA was eluted in 100 μ l of nuclease-free water.

    Article Title: Assessing the Performance of Dried-Blood-Spot DNA Extraction Methods in Next Generation Sequencing
    Article Snippet: .. DNA Extraction and Quantitation Genomic DNA used for Sanger sequence analysis was extracted from 200–250 μL whole blood (EDTA anticoagulant) using the Qiagen QIAamp® DNA blood mini spin columns and re-suspended in 100 μL of Tris-buffered EDTA (TE) (Qiagen, Valencia, CA, USA). .. The DNA was quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and diluted to 5–10 ng/μL for direct use for Sanger sequencing.

    Article Title: Isolation of Chlamydia Pneumoniae from Serum Samples of the Patients with Acute Coronary Syndrome
    Article Snippet: .. DNA isolation from infected HL cells Cells were harvested from 24 well plates and resuspended in 200 µl of lysis buffer and DNA was extracted using QIAamp DNA Blood Mini Kit (QIAGEN INC., Valencia, Calif.) according to the manual. .. DNA isolation from C.pneumoniae reference strain DNA was extracted from 100 µl of C. pneumoniae purified EB using reagents and protocol from QIAmp Blood Mini Kit (QIAGEN Inc., Valencia, Calif.).

    Article Title: Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology
    Article Snippet: .. These kits included the BiOstic bacteremia DNA isolation kit (MOBIO, Carlsbad, CA, USA), QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany), QIAamp UCP Pathogen Mini Kit (Qiagen, Hilden, Germany), TIANamp Blood DNA Kit (Tiangen, Beijing, China) and QIAamp cador Pathogen Mini Kit (Qiagen, Hilden, Germany). .. The benzyl alcohol-guanidine hydrochloride (Sigma-Aldrich, Saint Louis, USA) method was also tested .

    Isolation:

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids
    Article Snippet: .. In contrast, both Boman et al. ( ) and Layh-Schmitt et al. ( ) utilized a commercial kit (QIAmp Blood Mini Kit) for isolation of C. pneumoniae DNA, but they failed to detect bacterial DNA in CSFs of MS patients or found only a low positive rate. .. As shown in this study, the QIAmp Blood Mini Kit is not designed for isolation of bacterial DNA and was less effective for extracting bacterial DNA from CSFs, particularly when specimens contained few bacteria.

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: .. Among the various commonly available commercial kits for gDNA isolation, the kit from Qiagen has been shown to have columns with superior binding capability., Hence, the QIAamp DNA Blood Mini Kit (Qiagen) was chosen for comparison of the efficiency of the gDNA isolation with the described method here. ..

    Infection:

    Article Title: Isolation of Chlamydia Pneumoniae from Serum Samples of the Patients with Acute Coronary Syndrome
    Article Snippet: .. DNA isolation from infected HL cells Cells were harvested from 24 well plates and resuspended in 200 µl of lysis buffer and DNA was extracted using QIAamp DNA Blood Mini Kit (QIAGEN INC., Valencia, Calif.) according to the manual. .. DNA isolation from C.pneumoniae reference strain DNA was extracted from 100 µl of C. pneumoniae purified EB using reagents and protocol from QIAmp Blood Mini Kit (QIAGEN Inc., Valencia, Calif.).

    Quantitation Assay:

    Article Title: Assessing the Performance of Dried-Blood-Spot DNA Extraction Methods in Next Generation Sequencing
    Article Snippet: .. DNA Extraction and Quantitation Genomic DNA used for Sanger sequence analysis was extracted from 200–250 μL whole blood (EDTA anticoagulant) using the Qiagen QIAamp® DNA blood mini spin columns and re-suspended in 100 μL of Tris-buffered EDTA (TE) (Qiagen, Valencia, CA, USA). .. The DNA was quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and diluted to 5–10 ng/μL for direct use for Sanger sequencing.

    other:

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids
    Article Snippet: The QIAmp Blood Mini Kit is designed to extract mammalian DNA from biological specimens, including whole blood and body fluids, and has been utilized for extraction of C. pneumoniae DNA from CSF ( , ).

    Article Title: Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood
    Article Snippet: The increased sensitivity is most likely due to the fact that we could elute the DNA in a smaller volume using the QIAamp DNA Blood Mini kit than the QIAamp DNA Blood Midi kit.

    Mass Spectrometry:

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids
    Article Snippet: .. In contrast, both Boman et al. ( ) and Layh-Schmitt et al. ( ) utilized a commercial kit (QIAmp Blood Mini Kit) for isolation of C. pneumoniae DNA, but they failed to detect bacterial DNA in CSFs of MS patients or found only a low positive rate. .. As shown in this study, the QIAmp Blood Mini Kit is not designed for isolation of bacterial DNA and was less effective for extracting bacterial DNA from CSFs, particularly when specimens contained few bacteria.

    Sequencing:

    Article Title: Assessing the Performance of Dried-Blood-Spot DNA Extraction Methods in Next Generation Sequencing
    Article Snippet: .. DNA Extraction and Quantitation Genomic DNA used for Sanger sequence analysis was extracted from 200–250 μL whole blood (EDTA anticoagulant) using the Qiagen QIAamp® DNA blood mini spin columns and re-suspended in 100 μL of Tris-buffered EDTA (TE) (Qiagen, Valencia, CA, USA). .. The DNA was quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and diluted to 5–10 ng/μL for direct use for Sanger sequencing.

    Lysis:

    Article Title: Isolation of Chlamydia Pneumoniae from Serum Samples of the Patients with Acute Coronary Syndrome
    Article Snippet: .. DNA isolation from infected HL cells Cells were harvested from 24 well plates and resuspended in 200 µl of lysis buffer and DNA was extracted using QIAamp DNA Blood Mini Kit (QIAGEN INC., Valencia, Calif.) according to the manual. .. DNA isolation from C.pneumoniae reference strain DNA was extracted from 100 µl of C. pneumoniae purified EB using reagents and protocol from QIAmp Blood Mini Kit (QIAGEN Inc., Valencia, Calif.).

    Binding Assay:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: .. Among the various commonly available commercial kits for gDNA isolation, the kit from Qiagen has been shown to have columns with superior binding capability., Hence, the QIAamp DNA Blood Mini Kit (Qiagen) was chosen for comparison of the efficiency of the gDNA isolation with the described method here. ..

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  • 99
    Qiagen qiamp blood mini kit
    Optimization of annealing temperature and Mg 2+ concentration for PCR with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target <t>DNA</t> for PCR was extracted from C. pneumoniae with the <t>QIAmp</t> DNA Mini Kit.
    Qiamp Blood Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiamp blood mini kit/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    qiamp blood mini kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Optimization of annealing temperature and Mg 2+ concentration for PCR with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target DNA for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Optimization of annealing temperature and Mg 2+ concentration for PCR with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target DNA for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.

    Article Snippet: The QIAmp Blood Mini Kit is designed to extract mammalian DNA from biological specimens, including whole blood and body fluids, and has been utilized for extraction of C. pneumoniae DNA from CSF ( , ).

    Techniques: Concentration Assay, Polymerase Chain Reaction

    Detection sensitivity of PCR for omp1 versus the 16S rRNA gene. Two hundred microliters of the mock CSF containing a specific number of C. pneumoniae organisms (as indicated), was used for DNA extraction with the QIAmp DNA Mini Kit. A 2-μl portion of the 50-μl volume of DNA extracts was subjected to PCR. The optimized PCRs for omp1 and 16S rRNA gene were conducted (see Materials and Methods).

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Detection sensitivity of PCR for omp1 versus the 16S rRNA gene. Two hundred microliters of the mock CSF containing a specific number of C. pneumoniae organisms (as indicated), was used for DNA extraction with the QIAmp DNA Mini Kit. A 2-μl portion of the 50-μl volume of DNA extracts was subjected to PCR. The optimized PCRs for omp1 and 16S rRNA gene were conducted (see Materials and Methods).

    Article Snippet: The QIAmp Blood Mini Kit is designed to extract mammalian DNA from biological specimens, including whole blood and body fluids, and has been utilized for extraction of C. pneumoniae DNA from CSF ( , ).

    Techniques: Polymerase Chain Reaction, DNA Extraction

    Comparison of extraction efficacy of C. pneumoniae DNA from mock CSFs using two DNA extraction kits. The mock CSFs (200 μl) spiked with serially diluted bacteria were extracted either with the QIAmp DNA Mini Kit with a bacterial DNA extraction protocol or with the QIAmp DNA Blood Mini Kit. Two microliters of the extracted DNA (50 μl) was subjected to PCR with primers for omp1 . Results are representative of three experiments.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Comparison of extraction efficacy of C. pneumoniae DNA from mock CSFs using two DNA extraction kits. The mock CSFs (200 μl) spiked with serially diluted bacteria were extracted either with the QIAmp DNA Mini Kit with a bacterial DNA extraction protocol or with the QIAmp DNA Blood Mini Kit. Two microliters of the extracted DNA (50 μl) was subjected to PCR with primers for omp1 . Results are representative of three experiments.

    Article Snippet: The QIAmp Blood Mini Kit is designed to extract mammalian DNA from biological specimens, including whole blood and body fluids, and has been utilized for extraction of C. pneumoniae DNA from CSF ( , ).

    Techniques: DNA Extraction, Polymerase Chain Reaction

    Detection of C. pneumoniae DNA by PCR in CSFs obtained from patients with MS. Two hundred microliters of CSFs obtained from MS patients was processed for extraction of DNA utilizing the QIAmp DNA Mini Kit, and 2 μl of the resulting 50-μl DNA solution was subjected to PCR with primers for either omp1 or the 16S rRNA gene. Data are representative of three PCR experiments. Boxed numbers indicate CSFs obtained from the same patient at different time points. Each number is the sample number. M, molecular marker; PC, positive control for PCR; NC, negative control for PCR; IC, negative control for DNA extraction.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Detection of C. pneumoniae DNA by PCR in CSFs obtained from patients with MS. Two hundred microliters of CSFs obtained from MS patients was processed for extraction of DNA utilizing the QIAmp DNA Mini Kit, and 2 μl of the resulting 50-μl DNA solution was subjected to PCR with primers for either omp1 or the 16S rRNA gene. Data are representative of three PCR experiments. Boxed numbers indicate CSFs obtained from the same patient at different time points. Each number is the sample number. M, molecular marker; PC, positive control for PCR; NC, negative control for PCR; IC, negative control for DNA extraction.

    Article Snippet: The QIAmp Blood Mini Kit is designed to extract mammalian DNA from biological specimens, including whole blood and body fluids, and has been utilized for extraction of C. pneumoniae DNA from CSF ( , ).

    Techniques: Polymerase Chain Reaction, Mass Spectrometry, Marker, Positive Control, Negative Control, DNA Extraction