qiagen rnase a  (Qiagen)

 
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    Name:
    RNase A
    Description:
    For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
    Catalog Number:
    19101
    Price:
    215
    Category:
    RNase A
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    Structured Review

    Qiagen qiagen rnase a
    RNase A
    For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
    https://www.bioz.com/result/qiagen rnase a/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    qiagen rnase a - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "RNase A Promotes Proliferation of Neuronal Progenitor Cells via an ERK-Dependent Pathway"

    Article Title: RNase A Promotes Proliferation of Neuronal Progenitor Cells via an ERK-Dependent Pathway

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00428

    Proliferation inhibitor Ara-C blocks the effect of RNase A on NPC proliferation. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml Qiagen RNase A (R) at 1 DIV. Mock control (M) represents samples to which no extra material had been added. At 2 DIV, Ara-C (final 1 μM) was added into the culture. After two more days, cultures were harvested and immunostained using MAP2 and Nestin antibodies. DAPI staining was also performed to label cell nuclei. ( A ) Representative images. ( B ) Quantification of the percentage of Nestin + NPCs in total cells (indicated by DAPI stain, upper panel) and in the sum of MAP2 + neurons and Nestin + NPCs (lower panel). Five non-overlapping images under the microscope were randomly selected to determine the averages of cell numbers. Means and SD of three experiments are shown. Scale bars, 100 μm. Statistical analyses were performed using two-way ANOVA with Bonferroni's test. *** P
    Figure Legend Snippet: Proliferation inhibitor Ara-C blocks the effect of RNase A on NPC proliferation. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml Qiagen RNase A (R) at 1 DIV. Mock control (M) represents samples to which no extra material had been added. At 2 DIV, Ara-C (final 1 μM) was added into the culture. After two more days, cultures were harvested and immunostained using MAP2 and Nestin antibodies. DAPI staining was also performed to label cell nuclei. ( A ) Representative images. ( B ) Quantification of the percentage of Nestin + NPCs in total cells (indicated by DAPI stain, upper panel) and in the sum of MAP2 + neurons and Nestin + NPCs (lower panel). Five non-overlapping images under the microscope were randomly selected to determine the averages of cell numbers. Means and SD of three experiments are shown. Scale bars, 100 μm. Statistical analyses were performed using two-way ANOVA with Bonferroni's test. *** P

    Techniques Used: Acetylene Reduction Assay, Staining, Microscopy

    Qiagen RNase A also increases the NPC population in neuronal cultures. Qiagen RNase A (100 μg/ml) and BSA (100 μg/ml) were added into neuronal cultures at 1 DIV for 3 days. Mock control without adding any protein was also included. At 4 DIV, cells were fixed and immunostained with Nestin and MAP2 antibodies. Counter-staining with DAPI was performed to determine the total cell number. (A) Representative images. Scale bars, 50 μm. (B) Quantifications of the percentage of Nestin + cells in the total DAPI + cells (upper) and the sum of MAP2 + and Nestin + cells (bottom). Mean and SD of four experiments are shown. Statistical analyses were performed using one-way ANOVA. * P
    Figure Legend Snippet: Qiagen RNase A also increases the NPC population in neuronal cultures. Qiagen RNase A (100 μg/ml) and BSA (100 μg/ml) were added into neuronal cultures at 1 DIV for 3 days. Mock control without adding any protein was also included. At 4 DIV, cells were fixed and immunostained with Nestin and MAP2 antibodies. Counter-staining with DAPI was performed to determine the total cell number. (A) Representative images. Scale bars, 50 μm. (B) Quantifications of the percentage of Nestin + cells in the total DAPI + cells (upper) and the sum of MAP2 + and Nestin + cells (bottom). Mean and SD of four experiments are shown. Statistical analyses were performed using one-way ANOVA. * P

    Techniques Used: Staining

    Related Articles

    Irradiation:

    Article Title: Staufen1 promotes HCV replication by inhibiting protein kinase R and transporting viral RNA to the site of translation and replication in the cells
    Article Snippet: .. We treated the irradiated samples with RNase A (0.1 μg/μl; Qiagen, (Valencia, CA, USA) for 15 min at 37°C and resolved the crosslinked RNA–protein complexes on 8% sodium dodecyl sulfate (SDS) polyacrylamide gel. .. The labeled RNA–protein complex was visualized using a Typhoon scanner (Amersham).

    Transfection:

    Article Title: Production, Purification, and Capsid Stability of Rhinovirus C Types
    Article Snippet: .. In standard RV concentration protocols ( , ; ; ), lysates from cells transfected with A16, C2, C15, or C41 are incubated with N-lauroylsarcosine to release virus from cell membranes, RNAse A to eliminate transfection input RNA, and β-mercaptoethanol to inhibit RNAses. .. Viral particles are then pelleted by centrifugation (200,000 × g, 2h) through a 30% sucrose cushion.

    Mutagenesis:

    Article Title: Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation
    Article Snippet: .. This led to the identification of a positive charge patch around loop-2, loop-4, and β5 (on patch 2 surface in Figure ) that plays an important role in the RNA-dependent attenuation of deaminase activity, demonstrated by that the catalytic activity of an A3B containing mutation that reduces the surface charge within this region exhibited comparable catalytic activity in absence of RNase A when compared to treatment with RNase A (Figure ); a phenomena not observed for the wild type A3B or a mutant aimed at reducing the positively charged residues of α2-α4 (on patch 1 surface in Figure ). .. Of note, the catalytic activity of A3B-CD2 alone was also slightly increased after RNase A treatment , suggesting that RNA appears to interact CD2 domain as well to compete with the ssDNA substrate.

    Protease Inhibitor:

    Article Title: Oligomerization of HEXIM1 via 7SK snRNA and coiled-coil region directs the inhibition of P-TEFb
    Article Snippet: .. After 40 h, HeLa cells were harvested and lysed in 0.75 ml of lysis buffer [1% NP-40, 10 mM Tris–HCl, pH 7.4, 150 mM NaCl, 2 mM EDTA, 0.1% protease inhibitor and either 0.5% RNase inhibitor (Roche, Indianapolis, IN) or RNase A (100 µg/ml final concentration) (Qiagen, Hilden, Germany)] for 1 h at 4°C. .. The lysates were immunoprecipitated with anti-FLAG M2 beads for 2–4 h at 4°C and bound proteins were separated by SDS–PAGE electrophoresis and analyzed by immunoblotting with appropriate antibodies.

    Concentration Assay:

    Article Title: Oligomerization of HEXIM1 via 7SK snRNA and coiled-coil region directs the inhibition of P-TEFb
    Article Snippet: .. After 40 h, HeLa cells were harvested and lysed in 0.75 ml of lysis buffer [1% NP-40, 10 mM Tris–HCl, pH 7.4, 150 mM NaCl, 2 mM EDTA, 0.1% protease inhibitor and either 0.5% RNase inhibitor (Roche, Indianapolis, IN) or RNase A (100 µg/ml final concentration) (Qiagen, Hilden, Germany)] for 1 h at 4°C. .. The lysates were immunoprecipitated with anti-FLAG M2 beads for 2–4 h at 4°C and bound proteins were separated by SDS–PAGE electrophoresis and analyzed by immunoblotting with appropriate antibodies.

    Article Title: Production, Purification, and Capsid Stability of Rhinovirus C Types
    Article Snippet: .. In standard RV concentration protocols ( , ; ; ), lysates from cells transfected with A16, C2, C15, or C41 are incubated with N-lauroylsarcosine to release virus from cell membranes, RNAse A to eliminate transfection input RNA, and β-mercaptoethanol to inhibit RNAses. .. Viral particles are then pelleted by centrifugation (200,000 × g, 2h) through a 30% sucrose cushion.

    Incubation:

    Article Title: Identification of critical amino acid residues on human dihydrofolate reductase protein that mediate RNA recognition
    Article Snippet: .. After incubation at room temperature for 15 min, the reaction mixture was incubated with 300 U RNase T1 and 50 ng of RNase A for 15 min, after which heparin was added for an additional 10 min. .. The mixtures were filtered through a 0.22 µm nitrocellulose filter (Millipore).

    Article Title: Production, Purification, and Capsid Stability of Rhinovirus C Types
    Article Snippet: .. In standard RV concentration protocols ( , ; ; ), lysates from cells transfected with A16, C2, C15, or C41 are incubated with N-lauroylsarcosine to release virus from cell membranes, RNAse A to eliminate transfection input RNA, and β-mercaptoethanol to inhibit RNAses. .. Viral particles are then pelleted by centrifugation (200,000 × g, 2h) through a 30% sucrose cushion.

    other:

    Article Title: The Drosophila Helicase MLE Targets Hairpin Structures in Genomic Transcripts
    Article Snippet: The analysis was performed on 6 polytene chromosomes treated with RNase A and 8 control chromosomes. (PDF) Click here for additional data file.

    Article Title: Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation
    Article Snippet: Deamination assays of mutants that neutralized the positively charged regions on CD1 under the conditions with and without RNase A, allowed us to distinguish different interactions with ssDNA and RNA.

    Activity Assay:

    Article Title: Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation
    Article Snippet: .. This led to the identification of a positive charge patch around loop-2, loop-4, and β5 (on patch 2 surface in Figure ) that plays an important role in the RNA-dependent attenuation of deaminase activity, demonstrated by that the catalytic activity of an A3B containing mutation that reduces the surface charge within this region exhibited comparable catalytic activity in absence of RNase A when compared to treatment with RNase A (Figure ); a phenomena not observed for the wild type A3B or a mutant aimed at reducing the positively charged residues of α2-α4 (on patch 1 surface in Figure ). .. Of note, the catalytic activity of A3B-CD2 alone was also slightly increased after RNase A treatment , suggesting that RNA appears to interact CD2 domain as well to compete with the ssDNA substrate.

    Lysis:

    Article Title: Oligomerization of HEXIM1 via 7SK snRNA and coiled-coil region directs the inhibition of P-TEFb
    Article Snippet: .. After 40 h, HeLa cells were harvested and lysed in 0.75 ml of lysis buffer [1% NP-40, 10 mM Tris–HCl, pH 7.4, 150 mM NaCl, 2 mM EDTA, 0.1% protease inhibitor and either 0.5% RNase inhibitor (Roche, Indianapolis, IN) or RNase A (100 µg/ml final concentration) (Qiagen, Hilden, Germany)] for 1 h at 4°C. .. The lysates were immunoprecipitated with anti-FLAG M2 beads for 2–4 h at 4°C and bound proteins were separated by SDS–PAGE electrophoresis and analyzed by immunoblotting with appropriate antibodies.

    SDS Page:

    Article Title: Staufen1 promotes HCV replication by inhibiting protein kinase R and transporting viral RNA to the site of translation and replication in the cells
    Article Snippet: .. The crosslinked complexes were treated with RNase A, resolved by SDS-PAGE and visualized using a phosphorImager. .. Quantification of HCV RNA in the cell by quantitative real-time RT-PCR We used 1 μg of total cellular RNA to synthesize cDNA corresponding to HCV 5′ NTR and GAPDH mRNA by reverse transcription ( ).

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    Qiagen rnase inhibitor
    (a) SDS-PAGE of <t>NLP.</t> Lane 1, protein molecular mass marker; lane 2, untreated VSV NLP; lane 3, empty NLP after <t>RNase</t> A treatment; lane 4, reconstituted VSV NLP containing poly(rA) RNA. (b) Image of crystals of NLP with encapsidated poly(rG) RNA.
    Rnase Inhibitor, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor/product/Qiagen
    Average 98 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    99
    Qiagen qiagen rnase a
    Proliferation inhibitor Ara-C blocks the effect of <t>RNase</t> A on NPC proliferation. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml <t>Qiagen</t> RNase A (R) at 1 DIV. Mock control (M) represents samples to which no extra material had been added. At 2 DIV, Ara-C (final 1 μM) was added into the culture. After two more days, cultures were harvested and immunostained using MAP2 and Nestin antibodies. DAPI staining was also performed to label cell nuclei. ( A ) Representative images. ( B ) Quantification of the percentage of Nestin + NPCs in total cells (indicated by DAPI stain, upper panel) and in the sum of MAP2 + neurons and Nestin + NPCs (lower panel). Five non-overlapping images under the microscope were randomly selected to determine the averages of cell numbers. Means and SD of three experiments are shown. Scale bars, 100 μm. Statistical analyses were performed using two-way ANOVA with Bonferroni's test. *** P
    Qiagen Rnase A, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rnase a/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    qiagen rnase a - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    (a) SDS-PAGE of NLP. Lane 1, protein molecular mass marker; lane 2, untreated VSV NLP; lane 3, empty NLP after RNase A treatment; lane 4, reconstituted VSV NLP containing poly(rA) RNA. (b) Image of crystals of NLP with encapsidated poly(rG) RNA.

    Journal: Journal of Virology

    Article Title: Access to RNA Encapsidated in the Nucleocapsid of Vesicular Stomatitis Virus ▿

    doi: 10.1128/JVI.01927-10

    Figure Lengend Snippet: (a) SDS-PAGE of NLP. Lane 1, protein molecular mass marker; lane 2, untreated VSV NLP; lane 3, empty NLP after RNase A treatment; lane 4, reconstituted VSV NLP containing poly(rA) RNA. (b) Image of crystals of NLP with encapsidated poly(rG) RNA.

    Article Snippet: In the presence of RNase inhibitor (Qiagen), the empty NLP was incubated with poly(rA) (Midland) at a molar ratio of 1:5 for 15 min at 42°C.

    Techniques: SDS Page, Marker

    RNA analysis and electron microscopy of VSV nucleocapsid-like particles (NLP) and viral nucleocapsids digested with RNase A. (a) RNA electrophoresis. Purified VSV NLP treated with RNase A (1 mg/ml, final concentration) at room temperature (lane 3), 37°C

    Journal: Journal of Virology

    Article Title: Access to RNA Encapsidated in the Nucleocapsid of Vesicular Stomatitis Virus ▿

    doi: 10.1128/JVI.01927-10

    Figure Lengend Snippet: RNA analysis and electron microscopy of VSV nucleocapsid-like particles (NLP) and viral nucleocapsids digested with RNase A. (a) RNA electrophoresis. Purified VSV NLP treated with RNase A (1 mg/ml, final concentration) at room temperature (lane 3), 37°C

    Article Snippet: In the presence of RNase inhibitor (Qiagen), the empty NLP was incubated with poly(rA) (Midland) at a molar ratio of 1:5 for 15 min at 42°C.

    Techniques: Electron Microscopy, Electrophoresis, Purification, Concentration Assay

    Quantitative RT-PCR analysis of two miRNAs (miR-223-3p and let-7g-5p) identified by sequencing. The treatment of intact EVs with RNase before RNA extraction minimally altered Ct values of both miRNAs, strongly suggesting that these miRNAs were derived from the EVs-cargo and thereby protected from digestion by the membrane bilayer, whereas there was a large increase in Ct values when lysed EVs were similarly treated. Undetermined values (no detection by qRT-PCR) were assigned as Ct = 35. ( A , B ) Bar-graphs for miR-223-3p and let-7g-5p, showing Ct values for each treatment per sample; ( C , D ) Box-plots for miR-223-3p and let-7g-5p, showing Ct values per treatment (dots are results from each one of the five samples).

    Journal: Scientific Reports

    Article Title: A total transcriptome profiling method for plasma-derived extracellular vesicles: applications for liquid biopsies

    doi: 10.1038/s41598-017-14264-5

    Figure Lengend Snippet: Quantitative RT-PCR analysis of two miRNAs (miR-223-3p and let-7g-5p) identified by sequencing. The treatment of intact EVs with RNase before RNA extraction minimally altered Ct values of both miRNAs, strongly suggesting that these miRNAs were derived from the EVs-cargo and thereby protected from digestion by the membrane bilayer, whereas there was a large increase in Ct values when lysed EVs were similarly treated. Undetermined values (no detection by qRT-PCR) were assigned as Ct = 35. ( A , B ) Bar-graphs for miR-223-3p and let-7g-5p, showing Ct values for each treatment per sample; ( C , D ) Box-plots for miR-223-3p and let-7g-5p, showing Ct values per treatment (dots are results from each one of the five samples).

    Article Snippet: To verify the RNase-A activity, a parallel preparation was performed with the same samples wherein the EVs pellet was resuspended in lysis buffer (100 mM Tris, 5 mM EDTA, 0.2% SDS, 0.2 M NaCl, 0.1 ml/ml proteinase K), followed by proteinase-K inactivation at 90 °C for 5 min and RNase-A incubation and treatment with RNase inhibitor and RNA extraction as above. cDNAs were synthesized with miScript II RT Kit (Qiagen, USA) in 20 μl reactions containing 12 μl of RNA, 4 μl of 5X miScript HiSpec Buffer, 2 μl of 10X miScript Nucleics Mix and 2 μl of miScript Reverse Transcriptase Mix, which was incubated at 37 °C for 60 min and 95 °C for 5 min. cDNAs were pre-amplified with miScript PreAMP PCR Kit (Qiagen, USA) in 25 μl reaction containing: 5 μl of cDNA diluted 1:5, 5 μl of miScript PreAMP Buffer, 2 μl of HotStarTaq DNA Polymerase, 5 μl of pool of miRNA assays of interest, 7 μl of nuclease-free water, and 1 μl of miScript PreAMP Universal Primer.

    Techniques: Quantitative RT-PCR, Sequencing, RNA Extraction, Derivative Assay

    Size selection of ribosome footprints from RNase I treated monosome fractions. Representative gel images before and after gel cutting are shown. Brackets indicate the location of excised gel bands

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome

    doi: 10.1007/978-1-4939-7514-3_1

    Figure Lengend Snippet: Size selection of ribosome footprints from RNase I treated monosome fractions. Representative gel images before and after gel cutting are shown. Brackets indicate the location of excised gel bands

    Article Snippet: RNase I. RNase inhibitor. miRNeasy RNA purification kit (Qiagen, Valencia, CA, USA) ( see ).

    Techniques: Selection

    Proliferation inhibitor Ara-C blocks the effect of RNase A on NPC proliferation. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml Qiagen RNase A (R) at 1 DIV. Mock control (M) represents samples to which no extra material had been added. At 2 DIV, Ara-C (final 1 μM) was added into the culture. After two more days, cultures were harvested and immunostained using MAP2 and Nestin antibodies. DAPI staining was also performed to label cell nuclei. ( A ) Representative images. ( B ) Quantification of the percentage of Nestin + NPCs in total cells (indicated by DAPI stain, upper panel) and in the sum of MAP2 + neurons and Nestin + NPCs (lower panel). Five non-overlapping images under the microscope were randomly selected to determine the averages of cell numbers. Means and SD of three experiments are shown. Scale bars, 100 μm. Statistical analyses were performed using two-way ANOVA with Bonferroni's test. *** P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: RNase A Promotes Proliferation of Neuronal Progenitor Cells via an ERK-Dependent Pathway

    doi: 10.3389/fnmol.2018.00428

    Figure Lengend Snippet: Proliferation inhibitor Ara-C blocks the effect of RNase A on NPC proliferation. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml Qiagen RNase A (R) at 1 DIV. Mock control (M) represents samples to which no extra material had been added. At 2 DIV, Ara-C (final 1 μM) was added into the culture. After two more days, cultures were harvested and immunostained using MAP2 and Nestin antibodies. DAPI staining was also performed to label cell nuclei. ( A ) Representative images. ( B ) Quantification of the percentage of Nestin + NPCs in total cells (indicated by DAPI stain, upper panel) and in the sum of MAP2 + neurons and Nestin + NPCs (lower panel). Five non-overlapping images under the microscope were randomly selected to determine the averages of cell numbers. Means and SD of three experiments are shown. Scale bars, 100 μm. Statistical analyses were performed using two-way ANOVA with Bonferroni's test. *** P

    Article Snippet: Similar to RNase A from Invitrogen (Figure ), Qiagen RNase A also increased NPC population in neuronal cultures, as the percentage of Nestin+ NPCs in total cells was increased by RNase A compared to BSA control (Figures upper).

    Techniques: Acetylene Reduction Assay, Staining, Microscopy

    Qiagen RNase A also increases the NPC population in neuronal cultures. Qiagen RNase A (100 μg/ml) and BSA (100 μg/ml) were added into neuronal cultures at 1 DIV for 3 days. Mock control without adding any protein was also included. At 4 DIV, cells were fixed and immunostained with Nestin and MAP2 antibodies. Counter-staining with DAPI was performed to determine the total cell number. (A) Representative images. Scale bars, 50 μm. (B) Quantifications of the percentage of Nestin + cells in the total DAPI + cells (upper) and the sum of MAP2 + and Nestin + cells (bottom). Mean and SD of four experiments are shown. Statistical analyses were performed using one-way ANOVA. * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: RNase A Promotes Proliferation of Neuronal Progenitor Cells via an ERK-Dependent Pathway

    doi: 10.3389/fnmol.2018.00428

    Figure Lengend Snippet: Qiagen RNase A also increases the NPC population in neuronal cultures. Qiagen RNase A (100 μg/ml) and BSA (100 μg/ml) were added into neuronal cultures at 1 DIV for 3 days. Mock control without adding any protein was also included. At 4 DIV, cells were fixed and immunostained with Nestin and MAP2 antibodies. Counter-staining with DAPI was performed to determine the total cell number. (A) Representative images. Scale bars, 50 μm. (B) Quantifications of the percentage of Nestin + cells in the total DAPI + cells (upper) and the sum of MAP2 + and Nestin + cells (bottom). Mean and SD of four experiments are shown. Statistical analyses were performed using one-way ANOVA. * P

    Article Snippet: Similar to RNase A from Invitrogen (Figure ), Qiagen RNase A also increased NPC population in neuronal cultures, as the percentage of Nestin+ NPCs in total cells was increased by RNase A compared to BSA control (Figures upper).

    Techniques: Staining