qiagen rnase a (Qiagen)

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Name:

RNase A

Description:

For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation

Catalog Number:

19101

Price:

215

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RNase A

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Structured Review

Qiagen qiagen rnase a
RNase A

For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
https://www.bioz.com/result/qiagen rnase a/product/Qiagen
Average 99 stars, based on 6 article reviews
Price from $9.99 to $1999.99

qiagen rnase a - by Bioz Stars, 2021-01

99/100 stars

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Images

1) Product Images from "RNase A Promotes Proliferation of Neuronal Progenitor Cells via an ERK-Dependent Pathway"

Article Title: RNase A Promotes Proliferation of Neuronal Progenitor Cells via an ERK-Dependent Pathway

Journal: Frontiers in Molecular Neuroscience

doi: 10.3389/fnmol.2018.00428

Proliferation inhibitor Ara-C blocks the effect of RNase A on NPC proliferation. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml Qiagen RNase A (R) at 1 DIV. Mock control (M) represents samples to which no extra material had been added. At 2 DIV, Ara-C (final 1 μM) was added into the culture. After two more days, cultures were harvested and immunostained using MAP2 and Nestin antibodies. DAPI staining was also performed to label cell nuclei. ( A ) Representative images. ( B ) Quantification of the percentage of Nestin + NPCs in total cells (indicated by DAPI stain, upper panel) and in the sum of MAP2 + neurons and Nestin + NPCs (lower panel). Five non-overlapping images under the microscope were randomly selected to determine the averages of cell numbers. Means and SD of three experiments are shown. Scale bars, 100 μm. Statistical analyses were performed using two-way ANOVA with Bonferroni's test. *** P

Figure Legend Snippet: Proliferation inhibitor Ara-C blocks the effect of RNase A on NPC proliferation. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml Qiagen RNase A (R) at 1 DIV. Mock control (M) represents samples to which no extra material had been added. At 2 DIV, Ara-C (final 1 μM) was added into the culture. After two more days, cultures were harvested and immunostained using MAP2 and Nestin antibodies. DAPI staining was also performed to label cell nuclei. ( A ) Representative images. ( B ) Quantification of the percentage of Nestin + NPCs in total cells (indicated by DAPI stain, upper panel) and in the sum of MAP2 + neurons and Nestin + NPCs (lower panel). Five non-overlapping images under the microscope were randomly selected to determine the averages of cell numbers. Means and SD of three experiments are shown. Scale bars, 100 μm. Statistical analyses were performed using two-way ANOVA with Bonferroni's test. *** P

Techniques Used: Acetylene Reduction Assay, Staining, Microscopy

Qiagen RNase A also increases the NPC population in neuronal cultures. Qiagen RNase A (100 μg/ml) and BSA (100 μg/ml) were added into neuronal cultures at 1 DIV for 3 days. Mock control without adding any protein was also included. At 4 DIV, cells were fixed and immunostained with Nestin and MAP2 antibodies. Counter-staining with DAPI was performed to determine the total cell number. (A) Representative images. Scale bars, 50 μm. (B) Quantifications of the percentage of Nestin + cells in the total DAPI + cells (upper) and the sum of MAP2 + and Nestin + cells (bottom). Mean and SD of four experiments are shown. Statistical analyses were performed using one-way ANOVA. * P

Figure Legend Snippet: Qiagen RNase A also increases the NPC population in neuronal cultures. Qiagen RNase A (100 μg/ml) and BSA (100 μg/ml) were added into neuronal cultures at 1 DIV for 3 days. Mock control without adding any protein was also included. At 4 DIV, cells were fixed and immunostained with Nestin and MAP2 antibodies. Counter-staining with DAPI was performed to determine the total cell number. (A) Representative images. Scale bars, 50 μm. (B) Quantifications of the percentage of Nestin + cells in the total DAPI + cells (upper) and the sum of MAP2 + and Nestin + cells (bottom). Mean and SD of four experiments are shown. Statistical analyses were performed using one-way ANOVA. * P

Techniques Used: Staining

Mutagenesis:

Article Title: Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation
Article Snippet: .. This led to the identification of a positive charge patch around loop-2, loop-4, and β5 (on patch 2 surface in Figure ) that plays an important role in the RNA-dependent attenuation of deaminase activity, demonstrated by that the catalytic activity of an A3B containing mutation that reduces the surface charge within this region exhibited comparable catalytic activity in absence of RNase A when compared to treatment with RNase A (Figure ); a phenomena not observed for the wild type A3B or a mutant aimed at reducing the positively charged residues of α2-α4 (on patch 1 surface in Figure ). .. Of note, the catalytic activity of A3B-CD2 alone was also slightly increased after RNase A treatment , suggesting that RNA appears to interact CD2 domain as well to compete with the ssDNA substrate.

Purification:

Article Title: Circ_0008532 promotes bladder cancer progression by regulation of the miR-155-5p/miR-330-5p/MTGR1 axis
Article Snippet: .. RNase R digestionTotal RNA (2 μg) was incubated for 20 min at 37 °C with or without 3 U/μg of RNase R. The resulting RNA was purified using an RNeasy MinElute Cleanup Kit (Qiagen, Now York, USA). .. RNA binding protein immunoprecipitation assay (RIP) The RNA binding protein immunoprecipitation (RIP) assay was performed using the Magna RIP Kit (Millipore, USA) and Ago2 antibody (Cell Signaling Technology, USA) in accordance with the manufacturer’ instructions.

Incubation:

Article Title: Understanding the Structure, Multimerization, Subcellular Localization and mC Selectivity of a Genomic Mutator and Anti-HIV Factor APOBEC3H
Article Snippet: .. For RNase A treatment, the clear supernatant after lysis was incubated with 100 μg ml−1 RNase A on ice for 2 h before loading onto Superdex 200 10/300 GL column. .. Analysis of subcellular distribution of various A3H mutants The subcellular distribution of various A3H mutants in HEK293T cells was analyzed by cell fractionation to separate the cytosol and nuclear fractions, followed by SDS-PAGE and Western blot analysis.

Article Title: Identification of critical amino acid residues on human dihydrofolate reductase protein that mediate RNA recognition
Article Snippet: .. After incubation at room temperature for 15 min, the reaction mixture was incubated with 300 U RNase T1 and 50 ng of RNase A for 15 min, after which heparin was added for an additional 10 min. .. The mixtures were filtered through a 0.22 µm nitrocellulose filter (Millipore).

other:

Article Title: The Drosophila Helicase MLE Targets Hairpin Structures in Genomic Transcripts
Article Snippet: The analysis was performed on 6 polytene chromosomes treated with RNase A and 8 control chromosomes. (PDF) Click here for additional data file.

Article Title: Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation
Article Snippet: Deamination assays of mutants that neutralized the positively charged regions on CD1 under the conditions with and without RNase A, allowed us to distinguish different interactions with ssDNA and RNA.

Activity Assay:

Expressing:

Article Title: Understanding the Structure, Multimerization, Subcellular Localization and mC Selectivity of a Genomic Mutator and Anti-HIV Factor APOBEC3H
Article Snippet: .. To purify dimer and monomer of MBP-fused wild-type A3H hap II, E. coli cells expressing MBP-fused A3H hap II were harvested and lysed in buffer A (25 mM HEPES pH 7.5, 500 mM NaCl, 20 mM MgCl2 and 1 mM DTT) supplemented with 1 mg RNase A (Qiagen) per liter cells. .. The clear soluble fraction obtained after centrifugation was passed through amylose resin, washed with buffer B (50 mM HEPES pH 7.5, 500 mM NaCl, and 0.5 mM TCEP) in 0.5 M, 1 M, and 0.5 M NaCl gradient supplemented with 10 μg ml−1 RNase A, and eluted with buffer B supplemented with 40 mM maltose.

Lysis:

SDS Page:

Article Title: Staufen1 promotes HCV replication by inhibiting protein kinase R and transporting viral RNA to the site of translation and replication in the cells
Article Snippet: .. The crosslinked complexes were treated with RNase A, resolved by SDS-PAGE and visualized using a phosphorImager. .. Quantification of HCV RNA in the cell by quantitative real-time RT-PCR We used 1 μg of total cellular RNA to synthesize cDNA corresponding to HCV 5′ NTR and GAPDH mRNA by reverse transcription ( ).