qiagen puregene rnase  (Qiagen)

 
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    Name:
    RNase A Solution
    Description:
    For purification of archive quality DNA from a wide range of sample types Kit contents 650 l RNase A Solution Benefits Archive quality DNA for long term storage Reproducible DNA purification Convenient scalable purification proced
    Catalog Number:
    158922
    Price:
    83.5
    Category:
    Puregene Accessories
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    Structured Review

    Qiagen qiagen puregene rnase
    RNase A Solution
    For purification of archive quality DNA from a wide range of sample types Kit contents 650 l RNase A Solution Benefits Archive quality DNA for long term storage Reproducible DNA purification Convenient scalable purification proced
    https://www.bioz.com/result/qiagen puregene rnase/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiagen puregene rnase - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Fluorescence In Situ Hybridization:

    Article Title: In situ visualization of telomere elongation patterns in human cells
    Article Snippet: .. For FISH, the cells were rehydrated in 1× phosphate buffered saline (PBS) for 15 min, followed by fixation with 4% paraformaldehyde (in 1× PBS) for 2 min. After a wash in 1× PBS, cells were treated with RNase A (Qiagen catalogue no. 158922, diluted 1:100 in PBS) for approximately 2 h at room temperature. .. After three 1× PBS washes, cells were treated with 0.1 mg/mL pepsin (Sigma-Aldrich catalogue no. P7012, prepared fresh in 0.01 M HCl) at 37°C for 10 min.

    Immunoprecipitation:

    Article Title: Identification of Estrogen Receptor-Related Receptor Gamma as a Direct Transcriptional Target of Angiogenin
    Article Snippet: .. After RNase digestion and proteinase digestion, immunoprecipitated DNA was extracted with a QIAquick spin kit (Qiagen, Valencia, CA). .. The purified DNA was amplified by real-time PCR with the ABI7900 (Applied Biosystems, Foster City, CA, United States) and SYBR GREEN PCR Master Mix (Applied Biosystems).

    Incubation:

    Article Title: UGGT1 enhances enterovirus 71 pathogenicity by promoting viral RNA synthesis and viral replication
    Article Snippet: .. Proteins bound to the beads were eluted into a 1× sodium dodecyl sulfate (SDS) running buffer by heating at 95°C for 5 min. For RNase A treatment, 100 μL of RNase A in an RNase A working buffer (0.5 U) was added before any antibodies, and the samples were incubated at 37°C for 25 min. Total degradation RNA was extracted using an RNeasy kit (Qiagen, Chatsworth, CA), according to the manufacturer's recommendations, and gel analysis was conducted. .. For the JEV immunoprecipitation assay, BHK-21 cells were infected with JEV (strain T1P1) and incubated for 24 h, prior to conducting the immunoprecipitation assay.

    Article Title: Infrared Microspectroscopy Detects Protein Misfolding Cyclic Amplification (PMCA)-induced Conformational Alterations in Hamster Scrapie Progeny Seeds *
    Article Snippet: .. 0.6 μl of 0.5 m MgCl2 , 0.5 μl of RNase A solution (100 mg/ml; Qiagen), and 0.5 μl of Benzonase (Fluka) containing 2.5 units were added, and the sample was incubated overnight at room temperature on a shaking device. .. The following day, 2 μl of PK of a stock solution (1 mg/ml in ddH2 O) were added, and the sample was incubated for 1 h at room temperature on a shaking device.

    Isolation:

    Article Title: The helicase senataxin suppresses the antiviral transcriptional response and controls viral biogenesis
    Article Snippet: .. ChIP DNA was then isolated after RNase digestion and proteinase K digestion, using the QIAGEN MinElute kit (QIAGEN, 28004) and used for downstream applications. .. Statistical significance of ChIP qPCR analysis was determined using two-tailed Student’s paired t-test.

    Lysis:

    Article Title: Mass Spectrometric Quantitation of Pyridyloxobutyl DNA Phosphate Adducts in Rats Chronically Treated with N´-Nitrosonornicotine
    Article Snippet: .. The isotopically labelled internal standards [15 N3 ]Cp(POB)C and [13 C10 15 N2 ]Tp(POB)T were available from our previous studies., Cell lysis buffer (catalog number 158908), protein precipitation solution (catalog number 158912), proteinase K solution (catalog number 158920) and RNase A solution (catalog number 158924) were purchased from Qiagen. .. Ribonuclease T solution (catalog number R1003–500KU) was purchased from Sigma-Aldrich.

    Chromatin Immunoprecipitation:

    Article Title: The helicase senataxin suppresses the antiviral transcriptional response and controls viral biogenesis
    Article Snippet: .. ChIP DNA was then isolated after RNase digestion and proteinase K digestion, using the QIAGEN MinElute kit (QIAGEN, 28004) and used for downstream applications. .. Statistical significance of ChIP qPCR analysis was determined using two-tailed Student’s paired t-test.

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  • 99
    Qiagen rnase a treatment
    Co-precipitation of UGGT1 and the EVA71 3D viral polymerase. (A) At 6 hours post-infection, lysates from EVA71-infected or mock-infected cells were immunoprecipitated with anti-3D monoclonal antibody, and the precipitates were separated using SDS-PAGE, after which silver staining was applied for visualization. The seven labeled bands were excised, digested with trypsin, and analyzed by MALDI-TOF MS. (B) EVA71-infected and mock-infected cells were harvested and subjected to co-IP assays with anti-3D antibody (lanes 3 and 4) or mouse IgG (lanes 5 and 6); or anti-UGGT1 antibody (lanes 7 and 8) or rabbit IgG (lanes 9 and 10). The precipitates were analyzed using Western blotting with anti-UGGT1, anti-3D, anti-VP2, and anti-actin antibodies. (C) Cells were harvested at 6 h post-transfection, and lysates were treated with <t>RNase</t> A prior to being used in co-IP assays with an anti-3D antibody. Actin served as a loading control. Degradation of RNA was confirmed by RNA gel analysis. The precipitates were analyzed using Western blotting with anti-UGGT1, anti-ILF3, and anti-actin antibodies. (D) Membrane protein fractions were purified from EVA71-infected and mock-infected cells, and immunoprecipitation results with anti-3D antibody were analyzed by Western blotting with anti-3D, anti-3A, anti-VP2, and anti-UGGT1 antibodies. Expression of UGGT1, 3D, 3CD, 3AB, and 3A in the input lysate are shown. (E) EVA71-infected and mock-infected cells were fixed and stained with anti-UGGT1 and anti-3D antibodies at 6 h post-infection. An anti-UGGT1 antibody was used in panels 1 and 5, which were examined using a FITC filter. An anti-3D antibody was used in panels 2 and 6, which were examined using a rhodamine filter. Panels 3 and 7 display Hoechst 33258 staining results, and were examined using a 4’,6-diamidino-2-phenylindole (DAPI) filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images. (F) EVA71-infected or mock-infected cells were fixed and stained with antibodies against UGGT1 and double strand RNA. Results with the anti-double strand RNA antibody are shown in panels 1 and 5, which were examined using a rhodamine filter. Anti-UGGT1 antibody was used for panels 2 and 6, which were examined using an FITC filter. Panels 3 and 7 display Hoechst 33258 staining results, which were examined using a DAPI filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images.
    Rnase A Treatment, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase a treatment/product/Qiagen
    Average 99 stars, based on 1148 article reviews
    Price from $9.99 to $1999.99
    rnase a treatment - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Qiagen rnasea
    Co-precipitation of UGGT1 and the EVA71 3D viral polymerase. (A) At 6 hours post-infection, lysates from EVA71-infected or mock-infected cells were immunoprecipitated with anti-3D monoclonal antibody, and the precipitates were separated using SDS-PAGE, after which silver staining was applied for visualization. The seven labeled bands were excised, digested with trypsin, and analyzed by MALDI-TOF MS. (B) EVA71-infected and mock-infected cells were harvested and subjected to co-IP assays with anti-3D antibody (lanes 3 and 4) or mouse IgG (lanes 5 and 6); or anti-UGGT1 antibody (lanes 7 and 8) or rabbit IgG (lanes 9 and 10). The precipitates were analyzed using Western blotting with anti-UGGT1, anti-3D, anti-VP2, and anti-actin antibodies. (C) Cells were harvested at 6 h post-transfection, and lysates were treated with <t>RNase</t> A prior to being used in co-IP assays with an anti-3D antibody. Actin served as a loading control. Degradation of RNA was confirmed by RNA gel analysis. The precipitates were analyzed using Western blotting with anti-UGGT1, anti-ILF3, and anti-actin antibodies. (D) Membrane protein fractions were purified from EVA71-infected and mock-infected cells, and immunoprecipitation results with anti-3D antibody were analyzed by Western blotting with anti-3D, anti-3A, anti-VP2, and anti-UGGT1 antibodies. Expression of UGGT1, 3D, 3CD, 3AB, and 3A in the input lysate are shown. (E) EVA71-infected and mock-infected cells were fixed and stained with anti-UGGT1 and anti-3D antibodies at 6 h post-infection. An anti-UGGT1 antibody was used in panels 1 and 5, which were examined using a FITC filter. An anti-3D antibody was used in panels 2 and 6, which were examined using a rhodamine filter. Panels 3 and 7 display Hoechst 33258 staining results, and were examined using a 4’,6-diamidino-2-phenylindole (DAPI) filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images. (F) EVA71-infected or mock-infected cells were fixed and stained with antibodies against UGGT1 and double strand RNA. Results with the anti-double strand RNA antibody are shown in panels 1 and 5, which were examined using a rhodamine filter. Anti-UGGT1 antibody was used for panels 2 and 6, which were examined using an FITC filter. Panels 3 and 7 display Hoechst 33258 staining results, which were examined using a DAPI filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images.
    Rnasea, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnasea/product/Qiagen
    Average 99 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    rnasea - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Co-precipitation of UGGT1 and the EVA71 3D viral polymerase. (A) At 6 hours post-infection, lysates from EVA71-infected or mock-infected cells were immunoprecipitated with anti-3D monoclonal antibody, and the precipitates were separated using SDS-PAGE, after which silver staining was applied for visualization. The seven labeled bands were excised, digested with trypsin, and analyzed by MALDI-TOF MS. (B) EVA71-infected and mock-infected cells were harvested and subjected to co-IP assays with anti-3D antibody (lanes 3 and 4) or mouse IgG (lanes 5 and 6); or anti-UGGT1 antibody (lanes 7 and 8) or rabbit IgG (lanes 9 and 10). The precipitates were analyzed using Western blotting with anti-UGGT1, anti-3D, anti-VP2, and anti-actin antibodies. (C) Cells were harvested at 6 h post-transfection, and lysates were treated with RNase A prior to being used in co-IP assays with an anti-3D antibody. Actin served as a loading control. Degradation of RNA was confirmed by RNA gel analysis. The precipitates were analyzed using Western blotting with anti-UGGT1, anti-ILF3, and anti-actin antibodies. (D) Membrane protein fractions were purified from EVA71-infected and mock-infected cells, and immunoprecipitation results with anti-3D antibody were analyzed by Western blotting with anti-3D, anti-3A, anti-VP2, and anti-UGGT1 antibodies. Expression of UGGT1, 3D, 3CD, 3AB, and 3A in the input lysate are shown. (E) EVA71-infected and mock-infected cells were fixed and stained with anti-UGGT1 and anti-3D antibodies at 6 h post-infection. An anti-UGGT1 antibody was used in panels 1 and 5, which were examined using a FITC filter. An anti-3D antibody was used in panels 2 and 6, which were examined using a rhodamine filter. Panels 3 and 7 display Hoechst 33258 staining results, and were examined using a 4’,6-diamidino-2-phenylindole (DAPI) filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images. (F) EVA71-infected or mock-infected cells were fixed and stained with antibodies against UGGT1 and double strand RNA. Results with the anti-double strand RNA antibody are shown in panels 1 and 5, which were examined using a rhodamine filter. Anti-UGGT1 antibody was used for panels 2 and 6, which were examined using an FITC filter. Panels 3 and 7 display Hoechst 33258 staining results, which were examined using a DAPI filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images.

    Journal: PLoS Pathogens

    Article Title: UGGT1 enhances enterovirus 71 pathogenicity by promoting viral RNA synthesis and viral replication

    doi: 10.1371/journal.ppat.1006375

    Figure Lengend Snippet: Co-precipitation of UGGT1 and the EVA71 3D viral polymerase. (A) At 6 hours post-infection, lysates from EVA71-infected or mock-infected cells were immunoprecipitated with anti-3D monoclonal antibody, and the precipitates were separated using SDS-PAGE, after which silver staining was applied for visualization. The seven labeled bands were excised, digested with trypsin, and analyzed by MALDI-TOF MS. (B) EVA71-infected and mock-infected cells were harvested and subjected to co-IP assays with anti-3D antibody (lanes 3 and 4) or mouse IgG (lanes 5 and 6); or anti-UGGT1 antibody (lanes 7 and 8) or rabbit IgG (lanes 9 and 10). The precipitates were analyzed using Western blotting with anti-UGGT1, anti-3D, anti-VP2, and anti-actin antibodies. (C) Cells were harvested at 6 h post-transfection, and lysates were treated with RNase A prior to being used in co-IP assays with an anti-3D antibody. Actin served as a loading control. Degradation of RNA was confirmed by RNA gel analysis. The precipitates were analyzed using Western blotting with anti-UGGT1, anti-ILF3, and anti-actin antibodies. (D) Membrane protein fractions were purified from EVA71-infected and mock-infected cells, and immunoprecipitation results with anti-3D antibody were analyzed by Western blotting with anti-3D, anti-3A, anti-VP2, and anti-UGGT1 antibodies. Expression of UGGT1, 3D, 3CD, 3AB, and 3A in the input lysate are shown. (E) EVA71-infected and mock-infected cells were fixed and stained with anti-UGGT1 and anti-3D antibodies at 6 h post-infection. An anti-UGGT1 antibody was used in panels 1 and 5, which were examined using a FITC filter. An anti-3D antibody was used in panels 2 and 6, which were examined using a rhodamine filter. Panels 3 and 7 display Hoechst 33258 staining results, and were examined using a 4’,6-diamidino-2-phenylindole (DAPI) filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images. (F) EVA71-infected or mock-infected cells were fixed and stained with antibodies against UGGT1 and double strand RNA. Results with the anti-double strand RNA antibody are shown in panels 1 and 5, which were examined using a rhodamine filter. Anti-UGGT1 antibody was used for panels 2 and 6, which were examined using an FITC filter. Panels 3 and 7 display Hoechst 33258 staining results, which were examined using a DAPI filter. Panels 4 and 8 display merged rhodamine, FITC, and DAPI images.

    Article Snippet: Proteins bound to the beads were eluted into a 1× sodium dodecyl sulfate (SDS) running buffer by heating at 95°C for 5 min. For RNase A treatment, 100 μL of RNase A in an RNase A working buffer (0.5 U) was added before any antibodies, and the samples were incubated at 37°C for 25 min. Total degradation RNA was extracted using an RNeasy kit (Qiagen, Chatsworth, CA), according to the manufacturer's recommendations, and gel analysis was conducted.

    Techniques: Infection, Immunoprecipitation, SDS Page, Silver Staining, Labeling, Mass Spectrometry, Co-Immunoprecipitation Assay, Western Blot, Transfection, Purification, Expressing, Staining