qiaamp dna stool mini kit  (Qiagen)


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    Name:
    QIAamp DNA Stool Mini Kit
    Description:
    The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high quality genomic DNA human and bacterial from fresh or frozen stool samples The novel InhibitEX Buffer replaces cumbersome inhibitor removal tablets to efficiently remove PCR inhibitors commonly present in stool samples Fewer protocol steps streamlines your workflow and reduces hands on time during extraction The QIAamp Fast DNA Stool Mini Kit may be used with a microcentrifuge and may be automated on the QIAcube
    Catalog Number:
    51504
    Price:
    None
    Category:
    RNA DNA purification
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    Structured Review

    Qiagen qiaamp dna stool mini kit
    QIAamp DNA Stool Mini Kit
    The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high quality genomic DNA human and bacterial from fresh or frozen stool samples The novel InhibitEX Buffer replaces cumbersome inhibitor removal tablets to efficiently remove PCR inhibitors commonly present in stool samples Fewer protocol steps streamlines your workflow and reduces hands on time during extraction The QIAamp Fast DNA Stool Mini Kit may be used with a microcentrifuge and may be automated on the QIAcube
    https://www.bioz.com/result/qiaamp dna stool mini kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna stool mini kit - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples"

    Article Title: Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples

    Journal: BMC Research Notes

    doi: 10.1186/s13104-018-3300-2

    Ct values dot plots of PCR-positive samples for the seven eukaryotic enteric pathogens: Blastocystis spp. (n = 9), Cryptosporidium parvum / hominis (n = 8), Cyclospora cayetanensis (n = 10), Dientamoeba fragilis (n = 9), Giardia intestinalis (n = 8), Cystoisospora belli (n = 10) and Enterocytozoon bieneusi (n = 7) obtained with the two extraction procedures evaluated in the present study: EZ1 ® (EZ1) and QIAamp ® DNA Stool Mini Kit ® (QA). Mean values and standard deviation ranges for each pathogen are represented by large and short horizontal bars, respectively. Statistical significance is represented as **(p
    Figure Legend Snippet: Ct values dot plots of PCR-positive samples for the seven eukaryotic enteric pathogens: Blastocystis spp. (n = 9), Cryptosporidium parvum / hominis (n = 8), Cyclospora cayetanensis (n = 10), Dientamoeba fragilis (n = 9), Giardia intestinalis (n = 8), Cystoisospora belli (n = 10) and Enterocytozoon bieneusi (n = 7) obtained with the two extraction procedures evaluated in the present study: EZ1 ® (EZ1) and QIAamp ® DNA Stool Mini Kit ® (QA). Mean values and standard deviation ranges for each pathogen are represented by large and short horizontal bars, respectively. Statistical significance is represented as **(p

    Techniques Used: Polymerase Chain Reaction, Standard Deviation

    Related Articles

    other:

    Article Title: A novel protocol to isolate, detect and differentiate taeniid eggs in leafy greens and berries using real-time PCR with melting curve analysis
    Article Snippet: In our study, the FastDNA™ SPIN Kit for Soil outperformed the QIAamp® DNA Stool Mini Kit for the detection of Taenia sp. DNA from eggs in produce wash.

    Article Title: A novel protocol to isolate, detect and differentiate taeniid eggs in leafy greens and berries using real-time PCR with melting curve analysis
    Article Snippet: While there are many advantages of commercial kits, one drawback is the potential discontinuation of a product; which happened with the QIAamp® DNA Stool Mini Kit in the course of this study.

    DNA Extraction:

    Article Title: Evaluation and optimization of microbial DNA extraction from fecal samples of wild Antarctic bird species
    Article Snippet: Therefore, this kit was included in the current study, as well as five other kits widely used for fecal DNA extraction. .. The six DNA extraction kits evaluated were the following: PowerSoil DNA Isolation Kit (MO BIO Laboratories Inc., Carlsbad, CA, USA), Maxwell 16 Tissue DNA Purification Kit (Promega Biotech AB, Stockholm, Sweden), DNeasy Blood & Tissue Kit (Qiagen), QIAamp Fast DNA Stool Mini Kit (Qiagen), QIAamp DNA Stool Mini Kit (Qiagen) and QIAamp cador Pathogen Kit (Qiagen). ..

    Article Title: Evaluation and optimization of microbial DNA extraction from fecal samples of wild Antarctic bird species
    Article Snippet: .. The QIAamp DNA Stool Mini kit was included as reference, since this kit earlier has been used for avian fecal DNA extraction [ ]. .. However, the yield from the QIAamp DNA Stool Mini kit was low, even in combination with heat shock and bead beating ( ).

    Article Title: Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition
    Article Snippet: .. In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) ( and details below). .. In a second step, a variety of modifications to two Qiagen kits were examined, namely, the QIAamp DNA stool minikit (QIAStool) and the QIAamp Fast DNA stool minikit (QIAFast).

    DNA Purification:

    Article Title: Evaluation and optimization of microbial DNA extraction from fecal samples of wild Antarctic bird species
    Article Snippet: Therefore, this kit was included in the current study, as well as five other kits widely used for fecal DNA extraction. .. The six DNA extraction kits evaluated were the following: PowerSoil DNA Isolation Kit (MO BIO Laboratories Inc., Carlsbad, CA, USA), Maxwell 16 Tissue DNA Purification Kit (Promega Biotech AB, Stockholm, Sweden), DNeasy Blood & Tissue Kit (Qiagen), QIAamp Fast DNA Stool Mini Kit (Qiagen), QIAamp DNA Stool Mini Kit (Qiagen) and QIAamp cador Pathogen Kit (Qiagen). ..

    Purification:

    Article Title: Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition
    Article Snippet: .. In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) ( and details below). .. In a second step, a variety of modifications to two Qiagen kits were examined, namely, the QIAamp DNA stool minikit (QIAStool) and the QIAamp Fast DNA stool minikit (QIAFast).

    Article Title: Interleukin-23 drives innate and T cell-mediated intestinal inflammation
    Article Snippet: Quantitation of H. hepaticus using real-time PCR. .. DNA was purified from cecal contents taken from H. hepaticus –infected mice using the DNA Stool kit (QIAGEN). ..

    Incubation:

    Article Title: Multiplex Real-Time PCR Assay Using Scorpion Probes and DNA Capture for Genotype-Specific Detection of Giardia lamblia on Fecal Samples
    Article Snippet: .. DNA was then extracted using a QIAamp DNA Stool Mini kit (QIAGEN, Valencia, Calif.) per the manufacturer's instructions except that the suspension was incubated in the kit's stool lysis buffer at 95°C and a 3-min incubation with InhibitEx tablets was performed. ..

    Lysis:

    Article Title: Multiplex Real-Time PCR Assay Using Scorpion Probes and DNA Capture for Genotype-Specific Detection of Giardia lamblia on Fecal Samples
    Article Snippet: .. DNA was then extracted using a QIAamp DNA Stool Mini kit (QIAGEN, Valencia, Calif.) per the manufacturer's instructions except that the suspension was incubated in the kit's stool lysis buffer at 95°C and a 3-min incubation with InhibitEx tablets was performed. ..

    Infection:

    Article Title: Interleukin-23 drives innate and T cell-mediated intestinal inflammation
    Article Snippet: Quantitation of H. hepaticus using real-time PCR. .. DNA was purified from cecal contents taken from H. hepaticus –infected mice using the DNA Stool kit (QIAGEN). ..

    Mouse Assay:

    Article Title: Interleukin-23 drives innate and T cell-mediated intestinal inflammation
    Article Snippet: Quantitation of H. hepaticus using real-time PCR. .. DNA was purified from cecal contents taken from H. hepaticus –infected mice using the DNA Stool kit (QIAGEN). ..

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  • News
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  • 99
    Qiagen qiaamp dna stool mini kit
    Ct values dot plots of PCR-positive samples for the seven eukaryotic enteric pathogens: Blastocystis spp. (n = 9), Cryptosporidium parvum / hominis (n = 8), Cyclospora cayetanensis (n = 10), Dientamoeba fragilis (n = 9), Giardia intestinalis (n = 8), Cystoisospora belli (n = 10) and Enterocytozoon bieneusi (n = 7) obtained with the two extraction procedures evaluated in the present study: EZ1 ® (EZ1) and <t>QIAamp</t> ® <t>DNA</t> Stool Mini Kit ® (QA). Mean values and standard deviation ranges for each pathogen are represented by large and short horizontal bars, respectively. Statistical significance is represented as **(p
    Qiaamp Dna Stool Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp dna stool mini kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna stool mini kit - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Ct values dot plots of PCR-positive samples for the seven eukaryotic enteric pathogens: Blastocystis spp. (n = 9), Cryptosporidium parvum / hominis (n = 8), Cyclospora cayetanensis (n = 10), Dientamoeba fragilis (n = 9), Giardia intestinalis (n = 8), Cystoisospora belli (n = 10) and Enterocytozoon bieneusi (n = 7) obtained with the two extraction procedures evaluated in the present study: EZ1 ® (EZ1) and QIAamp ® DNA Stool Mini Kit ® (QA). Mean values and standard deviation ranges for each pathogen are represented by large and short horizontal bars, respectively. Statistical significance is represented as **(p

    Journal: BMC Research Notes

    Article Title: Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples

    doi: 10.1186/s13104-018-3300-2

    Figure Lengend Snippet: Ct values dot plots of PCR-positive samples for the seven eukaryotic enteric pathogens: Blastocystis spp. (n = 9), Cryptosporidium parvum / hominis (n = 8), Cyclospora cayetanensis (n = 10), Dientamoeba fragilis (n = 9), Giardia intestinalis (n = 8), Cystoisospora belli (n = 10) and Enterocytozoon bieneusi (n = 7) obtained with the two extraction procedures evaluated in the present study: EZ1 ® (EZ1) and QIAamp ® DNA Stool Mini Kit ® (QA). Mean values and standard deviation ranges for each pathogen are represented by large and short horizontal bars, respectively. Statistical significance is represented as **(p

    Article Snippet: We considered the EZ1® (Qiagen) procedure to be superior because of its higher throughput, shorter hands-on time, and lower contamination risk, associated with fewer manual preparation steps, than with the QIAamp® DNA Stool Mini Kit (Qiagen).

    Techniques: Polymerase Chain Reaction, Standard Deviation

    Effect of protocol modifications. (A) Pig feces was extracted using standard as well as modified protocols based on the QIAamp DNA stool minikit and QIAamp Fast DNA stool minikit. The modifications included bead beating, pretreatment of the sample, and transfer of the double amount of volume after cell lysis. In the bead beating step, different bead types were examined (for details, see Materials and Methods; Table 1 ). The alpha diversity (Chao 1 and Shannon index) was determined at OTU level, and the microbial community composition was examined at family level based on 16S rRNA gene profiling. (B) Selected standard and modified DNA extraction protocols were employed to extract DNA from human feces, pig feces, and sewage, and their DNA concentration was displayed in a star plot. The values indicate the averages from duplicate extractions.

    Journal: mSystems

    Article Title: Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

    doi: 10.1128/mSystems.00095-16

    Figure Lengend Snippet: Effect of protocol modifications. (A) Pig feces was extracted using standard as well as modified protocols based on the QIAamp DNA stool minikit and QIAamp Fast DNA stool minikit. The modifications included bead beating, pretreatment of the sample, and transfer of the double amount of volume after cell lysis. In the bead beating step, different bead types were examined (for details, see Materials and Methods; Table 1 ). The alpha diversity (Chao 1 and Shannon index) was determined at OTU level, and the microbial community composition was examined at family level based on 16S rRNA gene profiling. (B) Selected standard and modified DNA extraction protocols were employed to extract DNA from human feces, pig feces, and sewage, and their DNA concentration was displayed in a star plot. The values indicate the averages from duplicate extractions.

    Article Snippet: In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) ( and details below).

    Techniques: Modification, Lysis, DNA Extraction, Concentration Assay

    Comparison of DNA extraction methods. (A) Experimental design. Human feces, pig feces, and hospital sewage were extracted using seven different DNA extraction methods ( Table 1 ): InnuPure C16, MagNA Pure LC DNA isolation kit III, Easy-DNA gDNA purification kit, MP FastDNA Spin kit, PowerSoil DNA isolation kit, QIAamp DNA stool minikit, and QIAamp DNA stool minikit plus bead beating (for details, see Materials and Methods). DNA concentration, purity, and stability were examined, and microbial community composition was determined using 16S rRNA gene profiling and metagenomics (selected samples). (B) DNA from each method was dissolved in 100 µl solution, and DNA concentrations were determined using Qubit dsDNA BR assay kit measurements. Values represent averages from duplicate or triplicate DNA extractions (see also Table S1A in the supplemental material). (C) Ecological richness (Chao 1) and diversity (Shannon index) were determined based on contingency tables from 16S rRNA gene profiling and metagenomic sequencing data at OTU and species levels, respectively (see also Table S1B ).

    Journal: mSystems

    Article Title: Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

    doi: 10.1128/mSystems.00095-16

    Figure Lengend Snippet: Comparison of DNA extraction methods. (A) Experimental design. Human feces, pig feces, and hospital sewage were extracted using seven different DNA extraction methods ( Table 1 ): InnuPure C16, MagNA Pure LC DNA isolation kit III, Easy-DNA gDNA purification kit, MP FastDNA Spin kit, PowerSoil DNA isolation kit, QIAamp DNA stool minikit, and QIAamp DNA stool minikit plus bead beating (for details, see Materials and Methods). DNA concentration, purity, and stability were examined, and microbial community composition was determined using 16S rRNA gene profiling and metagenomics (selected samples). (B) DNA from each method was dissolved in 100 µl solution, and DNA concentrations were determined using Qubit dsDNA BR assay kit measurements. Values represent averages from duplicate or triplicate DNA extractions (see also Table S1A in the supplemental material). (C) Ecological richness (Chao 1) and diversity (Shannon index) were determined based on contingency tables from 16S rRNA gene profiling and metagenomic sequencing data at OTU and species levels, respectively (see also Table S1B ).

    Article Snippet: In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) ( and details below).

    Techniques: DNA Extraction, Purification, Concentration Assay, Sequencing

    Comparison of two DNA extraction kits to extract DNA from Taenia pisiformis eggs suspended in negative produce wash. Quantification cycle (Cq) values for each PCR are depicted as individual dots and a regression line is shown for each extraction kit. Cq values for the FastDNA™ SPIN Kit for Soil were significantly lower than for the QIAamp® DNA Stool Mini Kit ( P

    Journal: Parasites & Vectors

    Article Title: A novel protocol to isolate, detect and differentiate taeniid eggs in leafy greens and berries using real-time PCR with melting curve analysis

    doi: 10.1186/s13071-019-3834-8

    Figure Lengend Snippet: Comparison of two DNA extraction kits to extract DNA from Taenia pisiformis eggs suspended in negative produce wash. Quantification cycle (Cq) values for each PCR are depicted as individual dots and a regression line is shown for each extraction kit. Cq values for the FastDNA™ SPIN Kit for Soil were significantly lower than for the QIAamp® DNA Stool Mini Kit ( P

    Article Snippet: In our study, the FastDNA™ SPIN Kit for Soil outperformed the QIAamp® DNA Stool Mini Kit for the detection of Taenia sp. DNA from eggs in produce wash.

    Techniques: DNA Extraction, Polymerase Chain Reaction