qiaamp dna ffpe tissue kit  (Qiagen)


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    QIAamp DNA FFPE Tissue Kit
    Description:
    For purification of genomic DNA from formalin fixed paraffin embedded tissues Kit contents Qiagen QIAamp DNA FFPE Tissue Kit 50 preps 20 to 100L Elution Volume Up to 8 Sections Sample Formalin Fixed Paraffin Embedded Tissue Sample Silica Technology Genomic DNA Mitochondrial DNA Purification Spin Column Format Ideal for Real time PCR STR Analysis LMD PCR For Purification of Genomic DNA From Formalin fixed Paraffin embedded Tissues Includes 50 QIAamp MinElute Columns Proteinase K Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA Consistent high yields Complete removal of contaminants and inhibitor
    Catalog Number:
    56404
    Price:
    242
    Category:
    QIAamp DNA FFPE Tissue Kit
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    Structured Review

    Qiagen qiaamp dna ffpe tissue kit
    QIAamp DNA FFPE Tissue Kit
    For purification of genomic DNA from formalin fixed paraffin embedded tissues Kit contents Qiagen QIAamp DNA FFPE Tissue Kit 50 preps 20 to 100L Elution Volume Up to 8 Sections Sample Formalin Fixed Paraffin Embedded Tissue Sample Silica Technology Genomic DNA Mitochondrial DNA Purification Spin Column Format Ideal for Real time PCR STR Analysis LMD PCR For Purification of Genomic DNA From Formalin fixed Paraffin embedded Tissues Includes 50 QIAamp MinElute Columns Proteinase K Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA Consistent high yields Complete removal of contaminants and inhibitor
    https://www.bioz.com/result/qiaamp dna ffpe tissue kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna ffpe tissue kit - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum
    Article Snippet: .. Samples of tissue (1 cm3 ) were finely minced using a scalpel blade and then subjected to DNA isolation using either the QIAamp DNA FFPE Tissue Kit or the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Inc.). .. For the FFPE procedure, extraction with xylene was omitted but incubation at 90 °C to reverse formalin crosslinking was performed.

    Article Title: Critical Issues in Mycobiota Analysis
    Article Snippet: .. To highlight the influence of pre-analytics on ITS based mycobiota investigations we assessed the performance of DNA extraction from human skin FFPE samples (see Supplementary Table for sample information) with a commercially available kit (QIAamp DNA FFPE tissue kit, Qiagen) reported to be efficient for fungal DNA extraction out of FFPE material (Muñoz-Cadavid et al., ). .. We added a mechanical cell disruption step (bead-beating) to the procedure (MagnaLyser, Roche), since this step was shown to be crucial for complete lysis of microbial cells in specimens, significantly influencing correct community representation (de Boer et al., ; Reck et al., ).

    Article Title: Enhancement of Pathologist's Routine Practice: Reuse of DNA Extracted from Immunostained Formalin-fixed Paraffin-embedded (FFPE) Slides in Downstream Molecular Analysis of Cancer
    Article Snippet: Samples from the same patients were pooled in a 2-ml microcentrifuge tube and stored at –20˚C. .. DNA extraction from the IHC stained tissues was performed using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA) according to manufacturer's guidelines ( ). .. While the lysis step of this kit helps to release DNA and prevent possible inhibitory effects induced by formalin cross-linking of nucleic acids, the QIAamp spin columns allow washing and purification of samples followed by a final elution step in a new sterile tube to collect purified DNA (Figure 1).

    Article Title: Analytic validation and real-time clinical application of an amplicon-based targeted gene panel for advanced cancer
    Article Snippet: .. DNA extraction and QC DNA was isolated from cell lines and frozen tumors using QiaAMP DNA mini kit (Qiagen, Hilden, Germany), from blood using QiaAMP blood mini kit (Qiagen), and from FFPE tumors using QiaAMP DNA FFPE Tissue kit (Qiagen) per manufacturer's instructions. .. A Qubit fluorometer (ThermoFisher, Waltham, MA) was used to quantitate DNA.

    Article Title: Post-mortem testing; germline BRCA1/2 variant detection using archival FFPE non-tumor tissue. A new paradigm in genetic counseling
    Article Snippet: .. DNA extraction DNA was extracted from three tubes each containing 3 × 15 μ m FFPE tissue sections per sample using QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to QIAamp DNA FFPE Tissue Handbook with a few modifications ( www.qiagen.com ). .. The DNA was validated using 1% Tris-acetate EDTA gel electrophoresis and DNA concentrations were determined using PicoGreen (Invitrogen, Life Technologies Europe BV, Nærum, Denmark).

    Article Title: Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics
    Article Snippet: For local processing, FFPE tissue sections provided were reviewed by pathologists at the respective partner site and tumor areas were indicated for microdissection. .. DNA was extracted from microdissected tumor areas using either Maxwell® Rapid Sample Concentrator (RSC) Instrument and the Maxwell® 16 FFPE plus LEV DNA Purification Kit (Promega GmbH, Mannheim, Germany), High Pure FFPE DNA Isolation Kit (Roche Diagnostics, Mannheim, Germany), or QIAamp DNA FFPE Tissue Kit (QIAGEN, Hilden, Germany). .. DNA was quantified by Qubit dsDNA HS assay kit (QIAGEN), the NanoDrop instrument (Thermo Fisher Scientific, Dreieich, Germany), or the TaqMan® RNase P Detection Reagents Kit (Thermo Fisher Scientific).

    Formalin-fixed Paraffin-Embedded:

    Article Title: The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum
    Article Snippet: .. Samples of tissue (1 cm3 ) were finely minced using a scalpel blade and then subjected to DNA isolation using either the QIAamp DNA FFPE Tissue Kit or the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Inc.). .. For the FFPE procedure, extraction with xylene was omitted but incubation at 90 °C to reverse formalin crosslinking was performed.

    Article Title: Critical Issues in Mycobiota Analysis
    Article Snippet: .. To highlight the influence of pre-analytics on ITS based mycobiota investigations we assessed the performance of DNA extraction from human skin FFPE samples (see Supplementary Table for sample information) with a commercially available kit (QIAamp DNA FFPE tissue kit, Qiagen) reported to be efficient for fungal DNA extraction out of FFPE material (Muñoz-Cadavid et al., ). .. We added a mechanical cell disruption step (bead-beating) to the procedure (MagnaLyser, Roche), since this step was shown to be crucial for complete lysis of microbial cells in specimens, significantly influencing correct community representation (de Boer et al., ; Reck et al., ).

    Article Title: Enhancement of Pathologist's Routine Practice: Reuse of DNA Extracted from Immunostained Formalin-fixed Paraffin-embedded (FFPE) Slides in Downstream Molecular Analysis of Cancer
    Article Snippet: Samples from the same patients were pooled in a 2-ml microcentrifuge tube and stored at –20˚C. .. DNA extraction from the IHC stained tissues was performed using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA) according to manufacturer's guidelines ( ). .. While the lysis step of this kit helps to release DNA and prevent possible inhibitory effects induced by formalin cross-linking of nucleic acids, the QIAamp spin columns allow washing and purification of samples followed by a final elution step in a new sterile tube to collect purified DNA (Figure 1).

    Article Title: Analytic validation and real-time clinical application of an amplicon-based targeted gene panel for advanced cancer
    Article Snippet: .. DNA extraction and QC DNA was isolated from cell lines and frozen tumors using QiaAMP DNA mini kit (Qiagen, Hilden, Germany), from blood using QiaAMP blood mini kit (Qiagen), and from FFPE tumors using QiaAMP DNA FFPE Tissue kit (Qiagen) per manufacturer's instructions. .. A Qubit fluorometer (ThermoFisher, Waltham, MA) was used to quantitate DNA.

    Article Title: FGFR2 gene amplification and clinicopathological features in gastric cancer
    Article Snippet: .. Isolation of genomic DNA Genomic DNA samples were extracted from surgical specimens preserved as FFPE tissue using a QIAamp DNA Micro kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. Macro-dissection of the FFPE samples was performed to select a cancer region, which was marked by a pathologist after deparaffinisation.

    Article Title: Post-mortem testing; germline BRCA1/2 variant detection using archival FFPE non-tumor tissue. A new paradigm in genetic counseling
    Article Snippet: .. DNA extraction DNA was extracted from three tubes each containing 3 × 15 μ m FFPE tissue sections per sample using QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to QIAamp DNA FFPE Tissue Handbook with a few modifications ( www.qiagen.com ). .. The DNA was validated using 1% Tris-acetate EDTA gel electrophoresis and DNA concentrations were determined using PicoGreen (Invitrogen, Life Technologies Europe BV, Nærum, Denmark).

    Article Title: TMPRSS2, a SARS-CoV-2 internalization protease is downregulated in head and neck cancer patients
    Article Snippet: This kit includes also an internal control in order to check RNA extraction, PCR inhibition and application errors. .. Targeted DNA NGS Genomic DNA was extracted on the QIAcube® platform using the QIAamp DNA FFPE tissue kit (Qiagen) according to the manufacturer’s instructions. ..

    Article Title: Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics
    Article Snippet: For local processing, FFPE tissue sections provided were reviewed by pathologists at the respective partner site and tumor areas were indicated for microdissection. .. DNA was extracted from microdissected tumor areas using either Maxwell® Rapid Sample Concentrator (RSC) Instrument and the Maxwell® 16 FFPE plus LEV DNA Purification Kit (Promega GmbH, Mannheim, Germany), High Pure FFPE DNA Isolation Kit (Roche Diagnostics, Mannheim, Germany), or QIAamp DNA FFPE Tissue Kit (QIAGEN, Hilden, Germany). .. DNA was quantified by Qubit dsDNA HS assay kit (QIAGEN), the NanoDrop instrument (Thermo Fisher Scientific, Dreieich, Germany), or the TaqMan® RNase P Detection Reagents Kit (Thermo Fisher Scientific).

    Immunohistochemistry:

    Article Title: Enhancement of Pathologist's Routine Practice: Reuse of DNA Extracted from Immunostained Formalin-fixed Paraffin-embedded (FFPE) Slides in Downstream Molecular Analysis of Cancer
    Article Snippet: Samples from the same patients were pooled in a 2-ml microcentrifuge tube and stored at –20˚C. .. DNA extraction from the IHC stained tissues was performed using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA) according to manufacturer's guidelines ( ). .. While the lysis step of this kit helps to release DNA and prevent possible inhibitory effects induced by formalin cross-linking of nucleic acids, the QIAamp spin columns allow washing and purification of samples followed by a final elution step in a new sterile tube to collect purified DNA (Figure 1).

    Staining:

    Article Title: Enhancement of Pathologist's Routine Practice: Reuse of DNA Extracted from Immunostained Formalin-fixed Paraffin-embedded (FFPE) Slides in Downstream Molecular Analysis of Cancer
    Article Snippet: Samples from the same patients were pooled in a 2-ml microcentrifuge tube and stored at –20˚C. .. DNA extraction from the IHC stained tissues was performed using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA) according to manufacturer's guidelines ( ). .. While the lysis step of this kit helps to release DNA and prevent possible inhibitory effects induced by formalin cross-linking of nucleic acids, the QIAamp spin columns allow washing and purification of samples followed by a final elution step in a new sterile tube to collect purified DNA (Figure 1).

    Isolation:

    Article Title: Analytic validation and real-time clinical application of an amplicon-based targeted gene panel for advanced cancer
    Article Snippet: .. DNA extraction and QC DNA was isolated from cell lines and frozen tumors using QiaAMP DNA mini kit (Qiagen, Hilden, Germany), from blood using QiaAMP blood mini kit (Qiagen), and from FFPE tumors using QiaAMP DNA FFPE Tissue kit (Qiagen) per manufacturer's instructions. .. A Qubit fluorometer (ThermoFisher, Waltham, MA) was used to quantitate DNA.

    Article Title: FGFR2 gene amplification and clinicopathological features in gastric cancer
    Article Snippet: .. Isolation of genomic DNA Genomic DNA samples were extracted from surgical specimens preserved as FFPE tissue using a QIAamp DNA Micro kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. Macro-dissection of the FFPE samples was performed to select a cancer region, which was marked by a pathologist after deparaffinisation.

    Next-Generation Sequencing:

    Article Title: TMPRSS2, a SARS-CoV-2 internalization protease is downregulated in head and neck cancer patients
    Article Snippet: This kit includes also an internal control in order to check RNA extraction, PCR inhibition and application errors. .. Targeted DNA NGS Genomic DNA was extracted on the QIAcube® platform using the QIAamp DNA FFPE tissue kit (Qiagen) according to the manufacturer’s instructions. ..

    DNA Purification:

    Article Title: Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics
    Article Snippet: For local processing, FFPE tissue sections provided were reviewed by pathologists at the respective partner site and tumor areas were indicated for microdissection. .. DNA was extracted from microdissected tumor areas using either Maxwell® Rapid Sample Concentrator (RSC) Instrument and the Maxwell® 16 FFPE plus LEV DNA Purification Kit (Promega GmbH, Mannheim, Germany), High Pure FFPE DNA Isolation Kit (Roche Diagnostics, Mannheim, Germany), or QIAamp DNA FFPE Tissue Kit (QIAGEN, Hilden, Germany). .. DNA was quantified by Qubit dsDNA HS assay kit (QIAGEN), the NanoDrop instrument (Thermo Fisher Scientific, Dreieich, Germany), or the TaqMan® RNase P Detection Reagents Kit (Thermo Fisher Scientific).

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    • qiaamp dna ffpe tissue kit  (Qiagen)
    99
    Qiagen qiaamp dna ffpe tissue kit
    Analysis of 15 <t>FFPE</t> cancer samples with commercial cancer panels. Centrally as well as locally extracted <t>DNA</t> of molecularly pre-characterized cancer samples was sequenced by commercial cancer panels (CHPv2 and TSACP) at five different sequencing sites. Mutations ascertained by conventional Sanger or pyro-sequencing and reproduced by NGS are listed in supplementary Table 2. a Analysis of five Colon cancer samples. b Analysis of five Breast cancer samples. c Analysis of five Lung cancer samples, (#1–#5, respectively). Variant allelic frequencies, detected at different partner sites, are illustrated by bars as indicated. Samples not analyzed are indicated by “X”; variants not detected are indicated by open circles “ ○ .” WT wild type; a, b, and c PGM™ sequencing sites; d and e MiSeq™ sequencing sites
    Qiaamp Dna Ffpe Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp dna ffpe tissue kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna ffpe tissue kit - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier

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    Analysis of 15 FFPE cancer samples with commercial cancer panels. Centrally as well as locally extracted DNA of molecularly pre-characterized cancer samples was sequenced by commercial cancer panels (CHPv2 and TSACP) at five different sequencing sites. Mutations ascertained by conventional Sanger or pyro-sequencing and reproduced by NGS are listed in supplementary Table 2. a Analysis of five Colon cancer samples. b Analysis of five Breast cancer samples. c Analysis of five Lung cancer samples, (#1–#5, respectively). Variant allelic frequencies, detected at different partner sites, are illustrated by bars as indicated. Samples not analyzed are indicated by “X”; variants not detected are indicated by open circles “ ○ .” WT wild type; a, b, and c PGM™ sequencing sites; d and e MiSeq™ sequencing sites

    Journal: Virchows Archiv

    Article Title: Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics

    doi: 10.1007/s00428-017-2288-7

    Figure Lengend Snippet: Analysis of 15 FFPE cancer samples with commercial cancer panels. Centrally as well as locally extracted DNA of molecularly pre-characterized cancer samples was sequenced by commercial cancer panels (CHPv2 and TSACP) at five different sequencing sites. Mutations ascertained by conventional Sanger or pyro-sequencing and reproduced by NGS are listed in supplementary Table 2. a Analysis of five Colon cancer samples. b Analysis of five Breast cancer samples. c Analysis of five Lung cancer samples, (#1–#5, respectively). Variant allelic frequencies, detected at different partner sites, are illustrated by bars as indicated. Samples not analyzed are indicated by “X”; variants not detected are indicated by open circles “ ○ .” WT wild type; a, b, and c PGM™ sequencing sites; d and e MiSeq™ sequencing sites

    Article Snippet: DNA was extracted from microdissected tumor areas using either Maxwell® Rapid Sample Concentrator (RSC) Instrument and the Maxwell® 16 FFPE plus LEV DNA Purification Kit (Promega GmbH, Mannheim, Germany), High Pure FFPE DNA Isolation Kit (Roche Diagnostics, Mannheim, Germany), or QIAamp DNA FFPE Tissue Kit (QIAGEN, Hilden, Germany).

    Techniques: Formalin-fixed Paraffin-Embedded, Sequencing, Next-Generation Sequencing, Variant Assay

    Multicenter study design for targeted NGS. a A commercial gene panel (Cancer Hotspot panel 2, CHPv2, Thermo Fisher Scientific) was applied to DNA from the LoVo cell line at four distinct dilutions at three PGM™ sequencing sites (a, b, and c) to demonstrate exemplarily sensitivity. Bioinformatics was performed locally. b Genomic DNA from 15 molecularly pre-characterized tumor samples (five breast, five lung, and five colon cancer cases) was analyzed with commercially available and custom-designed cancer gene panels on PGM™ and MiSeq™ benchtop sequencers at seven sequencing sites (a, b, c, d, e, f, and g). FFPE tissue sections of the very same tumor samples were delivered to the sites a, b, d, and e for local microdissection, DNA Isolation, QC/quantification, and commercial panel sequencing. Partner site c did not receive tissue sections for local DNA extraction and applied only centrally extracted DNA to commercial (c*) and cancer-specific gene panel sequencing (c). Bioinformatics of commercial cancer panel-based data was performed individually whereas cancer-specific panel-based data were collected centrally and compiled

    Journal: Virchows Archiv

    Article Title: Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics

    doi: 10.1007/s00428-017-2288-7

    Figure Lengend Snippet: Multicenter study design for targeted NGS. a A commercial gene panel (Cancer Hotspot panel 2, CHPv2, Thermo Fisher Scientific) was applied to DNA from the LoVo cell line at four distinct dilutions at three PGM™ sequencing sites (a, b, and c) to demonstrate exemplarily sensitivity. Bioinformatics was performed locally. b Genomic DNA from 15 molecularly pre-characterized tumor samples (five breast, five lung, and five colon cancer cases) was analyzed with commercially available and custom-designed cancer gene panels on PGM™ and MiSeq™ benchtop sequencers at seven sequencing sites (a, b, c, d, e, f, and g). FFPE tissue sections of the very same tumor samples were delivered to the sites a, b, d, and e for local microdissection, DNA Isolation, QC/quantification, and commercial panel sequencing. Partner site c did not receive tissue sections for local DNA extraction and applied only centrally extracted DNA to commercial (c*) and cancer-specific gene panel sequencing (c). Bioinformatics of commercial cancer panel-based data was performed individually whereas cancer-specific panel-based data were collected centrally and compiled

    Article Snippet: DNA was extracted from microdissected tumor areas using either Maxwell® Rapid Sample Concentrator (RSC) Instrument and the Maxwell® 16 FFPE plus LEV DNA Purification Kit (Promega GmbH, Mannheim, Germany), High Pure FFPE DNA Isolation Kit (Roche Diagnostics, Mannheim, Germany), or QIAamp DNA FFPE Tissue Kit (QIAGEN, Hilden, Germany).

    Techniques: Next-Generation Sequencing, Sequencing, Formalin-fixed Paraffin-Embedded, Laser Capture Microdissection, DNA Extraction

    Gel electrophoresis of CTNNB1 gene (190 bp) from DNA retrieved from already immunostained FFPE IHC slides. L, Gel ladder as a control; lanes 1 to 11, numbers' of assessed samples; IHC, immunohisochemistry, FFPE, formalin-fixed, paraffin-embedded

    Journal: Cancer Genomics & Proteomics

    Article Title: Enhancement of Pathologist's Routine Practice: Reuse of DNA Extracted from Immunostained Formalin-fixed Paraffin-embedded (FFPE) Slides in Downstream Molecular Analysis of Cancer

    doi:

    Figure Lengend Snippet: Gel electrophoresis of CTNNB1 gene (190 bp) from DNA retrieved from already immunostained FFPE IHC slides. L, Gel ladder as a control; lanes 1 to 11, numbers' of assessed samples; IHC, immunohisochemistry, FFPE, formalin-fixed, paraffin-embedded

    Article Snippet: DNA extraction from the IHC stained tissues was performed using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA) according to manufacturer's guidelines ( ).

    Techniques: Nucleic Acid Electrophoresis, Formalin-fixed Paraffin-Embedded, Immunohistochemistry

    Agarose gel electrophoresis of DNA isolated from cadaver tissues. MW = GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific). Lane 1: Heart DNA extracted with DNeasy Blood Tissue Kit. Lane 2: Liver DNA extracted with DNeasy Blood Tissue Kit. Lane 3: Heart DNA extracted with FFPE kit. Lane 4: Liver DNA extracted with FFPE kit

    Journal: BMC Medical Genomics

    Article Title: The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum

    doi: 10.1186/s12920-016-0223-4

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA isolated from cadaver tissues. MW = GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific). Lane 1: Heart DNA extracted with DNeasy Blood Tissue Kit. Lane 2: Liver DNA extracted with DNeasy Blood Tissue Kit. Lane 3: Heart DNA extracted with FFPE kit. Lane 4: Liver DNA extracted with FFPE kit

    Article Snippet: Samples of tissue (1 cm3 ) were finely minced using a scalpel blade and then subjected to DNA isolation using either the QIAamp DNA FFPE Tissue Kit or the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Inc.).

    Techniques: Agarose Gel Electrophoresis, Isolation, Formalin-fixed Paraffin-Embedded

    Case report: COVID-19 positive HNSCC patient. a Clinical history of COVID-19 positive HNSCC patient: at day 0 patient underwent surgical resection of the tumor that was formalin fixed and paraffin embedded. 2 days after surgery the patient developed pneumonia symptoms and nasopharyngeal swab SARS-CoV-2 test at day 6 was positive. SARS-CoV-2 test was also performed in FFPE tumoral tissue and resulted to be negative. b Clinical characteristics of COVID-19 positive HNSCC patient. c Well differentiated squamous cell carcinoma (G1) showing areas of keratinization with infiltration of muscular layer. d DNA sequencing results. e Immunostaining for p53 protein expression in COVID-19 positive HNSCC patient. f Real-time PCR curves from SARS-CoV-2 test run of RNA extracted from FFPE tumor tissue of HNSCC patient and from FFPE non-tumorous and tumor lung tissues of a lung cancer patient enrolled in 2011. The internal control was indicated by the red arrow. g qRT-PCR analysis of TMPRSS2 in 5 normal lung tissues (N) and 5 matched lung cancer tissues plus COVID-19 positive HNSCC tissue (T). h qRT-PCR analysis of miR-503-5p in 9 non-tumorous lung tissues (N) and 9 matched lung cancer tissues plus COVID-19 positive HNSCC tissue (T)

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: TMPRSS2, a SARS-CoV-2 internalization protease is downregulated in head and neck cancer patients

    doi: 10.1186/s13046-020-01708-6

    Figure Lengend Snippet: Case report: COVID-19 positive HNSCC patient. a Clinical history of COVID-19 positive HNSCC patient: at day 0 patient underwent surgical resection of the tumor that was formalin fixed and paraffin embedded. 2 days after surgery the patient developed pneumonia symptoms and nasopharyngeal swab SARS-CoV-2 test at day 6 was positive. SARS-CoV-2 test was also performed in FFPE tumoral tissue and resulted to be negative. b Clinical characteristics of COVID-19 positive HNSCC patient. c Well differentiated squamous cell carcinoma (G1) showing areas of keratinization with infiltration of muscular layer. d DNA sequencing results. e Immunostaining for p53 protein expression in COVID-19 positive HNSCC patient. f Real-time PCR curves from SARS-CoV-2 test run of RNA extracted from FFPE tumor tissue of HNSCC patient and from FFPE non-tumorous and tumor lung tissues of a lung cancer patient enrolled in 2011. The internal control was indicated by the red arrow. g qRT-PCR analysis of TMPRSS2 in 5 normal lung tissues (N) and 5 matched lung cancer tissues plus COVID-19 positive HNSCC tissue (T). h qRT-PCR analysis of miR-503-5p in 9 non-tumorous lung tissues (N) and 9 matched lung cancer tissues plus COVID-19 positive HNSCC tissue (T)

    Article Snippet: Targeted DNA NGS Genomic DNA was extracted on the QIAcube® platform using the QIAamp DNA FFPE tissue kit (Qiagen) according to the manufacturer’s instructions.

    Techniques: Formalin-fixed Paraffin-Embedded, DNA Sequencing, Immunostaining, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR