qiaamp dna blood mini kit  (Qiagen)


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    Name:
    QIAamp DNA Blood Mini Kit
    Description:
    For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
    Catalog Number:
    51104
    Price:
    157
    Category:
    QIAamp DNA Blood Mini Kit
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    Structured Review

    Qiagen qiaamp dna blood mini kit
    QIAamp DNA Blood Mini Kit
    For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
    https://www.bioz.com/result/qiaamp dna blood mini kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna blood mini kit - by Bioz Stars, 2021-03
    97/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification
    Article Snippet: Equal volumes of these dilution series were further used for genomic DNA extraction by microwave irradiation and, subsequently, for standard PCR and tailored LAMP assays. .. DNA extraction: microwave irradiation First, DNA was extracted from whole blood samples as well as from the cultured parasites of the dilution series using the conventional QIAamp DNA mini blood kit based extraction procedure (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Next, three standard operation procedures (SOPs) were established for microwave irradiation based DNA extraction (MDA oven, model number: MW17M70G-AU, 230 V, 50 HZ).

    Irradiation:

    Article Title: A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification
    Article Snippet: Equal volumes of these dilution series were further used for genomic DNA extraction by microwave irradiation and, subsequently, for standard PCR and tailored LAMP assays. .. DNA extraction: microwave irradiation First, DNA was extracted from whole blood samples as well as from the cultured parasites of the dilution series using the conventional QIAamp DNA mini blood kit based extraction procedure (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Next, three standard operation procedures (SOPs) were established for microwave irradiation based DNA extraction (MDA oven, model number: MW17M70G-AU, 230 V, 50 HZ).

    Cell Culture:

    Article Title: A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification
    Article Snippet: Equal volumes of these dilution series were further used for genomic DNA extraction by microwave irradiation and, subsequently, for standard PCR and tailored LAMP assays. .. DNA extraction: microwave irradiation First, DNA was extracted from whole blood samples as well as from the cultured parasites of the dilution series using the conventional QIAamp DNA mini blood kit based extraction procedure (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Next, three standard operation procedures (SOPs) were established for microwave irradiation based DNA extraction (MDA oven, model number: MW17M70G-AU, 230 V, 50 HZ).

    other:

    Article Title: Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots
    Article Snippet: The control Chelex® protocol yielded 590% more DNA than the QIAamp® DNA Blood Mini Kit .

    Isolation:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: Two hundred microliters of Tris-EDTA (10 mM Tris-Cl and 1 mM EDTA, pH 8.0) was added to the tube containing the membrane and incubated at room temperature for 2 h with occasional finger tapping ( , Step 13) to elute the membrane-bound gDNA. .. Among the various commonly available commercial kits for gDNA isolation, the kit from Qiagen has been shown to have columns with superior binding capability., Hence, the QIAamp DNA Blood Mini Kit (Qiagen) was chosen for comparison of the efficiency of the gDNA isolation with the described method here. ..

    Article Title: Aberrant ZNF423 impedes B cell differentiation and is linked to adverse outcome of ETV6-RUNX1 negative B precursor acute lymphoblastic leukemia
    Article Snippet: .. Genomic DNA was isolated from initial ALL samples and matched MNC from remission BM using the QIAGEN DNA Blood Mini kit following the manufacturer’s instructions. .. Sample preparation, hybridization, and staining were performed according to the manufacturer’s standard protocol for Affymetrix GenomeWide Human SNP 6.0 microarrays.

    Binding Assay:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: Two hundred microliters of Tris-EDTA (10 mM Tris-Cl and 1 mM EDTA, pH 8.0) was added to the tube containing the membrane and incubated at room temperature for 2 h with occasional finger tapping ( , Step 13) to elute the membrane-bound gDNA. .. Among the various commonly available commercial kits for gDNA isolation, the kit from Qiagen has been shown to have columns with superior binding capability., Hence, the QIAamp DNA Blood Mini Kit (Qiagen) was chosen for comparison of the efficiency of the gDNA isolation with the described method here. ..

    Amplification:

    Article Title: Inflammatory Genital Infections Mitigate a Severe Genetic Bottleneck in Heterosexual Transmission of Subtype A and C HIV-1
    Article Snippet: For individuals identified as antibody negative and p24 antigen positive, the estimated date of infection was determined as 22 days prior to that date (Fiebig stage II), and finally for individuals identified as viral RNA positive and antibody/p24 antigen negative, the estimated date of infection was determined as 17 days prior to this sample date (Fiebig stage I). .. End-Point Dilution, Single Genome Amplification of HIV-1 env Genes For amplification of proviral HIV-1 env genes, genomic DNA was extracted from uncultured peripheral blood mononuclear cells (PBMC) using the Qiagen DNA Blood Mini Kit. .. For amplification of plasma HIV-1 env genes, RNA was purified from plasma samples using the Qiagen Viral Isolation Kit and cDNA prepared using the SuperScript III reverse transcriptase according to the manufacturer's instructions.

    Article Title: JAK2V617F Allele Burden Measurement in Peripheral Blood of Iranian Patients with Myeloproliferative Neoplasms and Effect of Hydroxyurea on JAK2V617F Allele Burden
    Article Snippet: The patients were selected from Hematology-Oncology and BMT Research Center at Shariati and Imam Khomeini Hospital affiliated with Tehran University of Medical Science. .. The study was approved by our institutional review board and written informed consent was obtained from all patients. (Ethical code: ir.tums.horcsct.1394.103.7) JAK2 V617F screening by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) Genomic DNA was prepared from leukocytes using the DNA blood mini kit (Qiagen, Germany). .. Mutation analysis of the JAK2 V617F was initially performed using ARMS-PCR.

    Two Tailed Test:

    Article Title: Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots
    Article Snippet: Total gEq recovered for all conditions was calculated as the concentration of the sample by beta-globin qPCR multiplied by the final elution volume. .. We compared the mean efficiency of the Chelex® control method to the QIAamp® DNA Blood Mini Kit using a two-tailed Student’s t -test. ..

    Mutagenesis:

    Article Title: JAK2V617F Allele Burden Measurement in Peripheral Blood of Iranian Patients with Myeloproliferative Neoplasms and Effect of Hydroxyurea on JAK2V617F Allele Burden
    Article Snippet: The patients were selected from Hematology-Oncology and BMT Research Center at Shariati and Imam Khomeini Hospital affiliated with Tehran University of Medical Science. .. The study was approved by our institutional review board and written informed consent was obtained from all patients. (Ethical code: ir.tums.horcsct.1394.103.7) JAK2 V617F screening by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) Genomic DNA was prepared from leukocytes using the DNA blood mini kit (Qiagen, Germany). .. Mutation analysis of the JAK2 V617F was initially performed using ARMS-PCR.

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    Qiagen dna blood mini kit
    Transmission of multiple variants. Aligned nucleotide sequences for linked donors (green circles) and linked recipients (blue circles) were used to generate neighbor-joining trees for individual transmission pairs (A) RW57 and (B) ZM229. Horizontal branch lengths are drawn to scale with scale bar representing 1% divergence. Open circles represent sequences derived from uncultured <t>PBMC</t> <t>DNA</t> and closed circles represent sequences derived from plasma RNA. PBMC samples were unavailable for ZM229; therefore, only plasma sequences were derived for this transmission pair. Asterisks indicate branches with bootstrap values greater than 0.99.
    Dna Blood Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna blood mini kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna blood mini kit - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

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    Transmission of multiple variants. Aligned nucleotide sequences for linked donors (green circles) and linked recipients (blue circles) were used to generate neighbor-joining trees for individual transmission pairs (A) RW57 and (B) ZM229. Horizontal branch lengths are drawn to scale with scale bar representing 1% divergence. Open circles represent sequences derived from uncultured PBMC DNA and closed circles represent sequences derived from plasma RNA. PBMC samples were unavailable for ZM229; therefore, only plasma sequences were derived for this transmission pair. Asterisks indicate branches with bootstrap values greater than 0.99.

    Journal: PLoS Pathogens

    Article Title: Inflammatory Genital Infections Mitigate a Severe Genetic Bottleneck in Heterosexual Transmission of Subtype A and C HIV-1

    doi: 10.1371/journal.ppat.1000274

    Figure Lengend Snippet: Transmission of multiple variants. Aligned nucleotide sequences for linked donors (green circles) and linked recipients (blue circles) were used to generate neighbor-joining trees for individual transmission pairs (A) RW57 and (B) ZM229. Horizontal branch lengths are drawn to scale with scale bar representing 1% divergence. Open circles represent sequences derived from uncultured PBMC DNA and closed circles represent sequences derived from plasma RNA. PBMC samples were unavailable for ZM229; therefore, only plasma sequences were derived for this transmission pair. Asterisks indicate branches with bootstrap values greater than 0.99.

    Article Snippet: End-Point Dilution, Single Genome Amplification of HIV-1 env Genes For amplification of proviral HIV-1 env genes, genomic DNA was extracted from uncultured peripheral blood mononuclear cells (PBMC) using the Qiagen DNA Blood Mini Kit.

    Techniques: Transmission Assay, Derivative Assay

    ZNF423 expression is regulated by DNA methylation. (A) Transactivation of ZNF423 isoform-specific promoters in dependence of central CGI. Reporter gene assay using ZNF423 promoters (α and β) with or without CGI after transfection in 293T cells. Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars represent SD from three technical replicates. Significance is calculated by Student’s t test, comparing indicated samples (***, P ≤ 0.001). Data were reproduced in three independent experiments. (B) Methylation map of CpGs in the central CGI at the ZNF423 locus. Genomic DNA from primary ALL ( n = 58), matched BM MNCs in complete continuous remission (MNC [CCR]; n = 58), H1, HES2 ESC lines, and normal hematopoietic cells at various stages of differentiation ( n = 42) from healthy donors were sequenced after bisulfite conversion. Each row represents one cytosine in a CG dinucleotide of the analyzed sequence. For cell lineage abbreviations, refer to Figure 1 . Significance is calculated by Student’s t test, comparing primary ALL to immunologically characterized control samples (in brackets; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001). Color code represents degree of methylation in percent. White boxes indicate no sequencing data available. (C) ZNF423 promoter activity in dependence of CPG mutational status. Disruption of CpGs was performed by site-directed mutagenesis as indicated by red marks. Combined deletion mutant (pCpGL-CGI-del_comb_α-prom) represents deletions at CGI positions 102, 183, 220, 250. Wild-type and mutated plasmids were treated with DNA-methylase M.SssI and transfected into 293T. Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars represent SD from three technical replicates. Significance is calculated by Student’s t test, comparing M.SssI-treated wild-type and mutated samples (***, P ≤ 0.001). Data were reproduced in three independent experiments. (D) DNA methylation pattern of central CGI. Diagram was created with BiQ Analyzer software from Max-Planck-Institute of Informatics. Drawing is to scale. White lollipop, unmethylated; black lollipop, methylated. Marked CpGs refer to heatmap in Fig. 4 B . (E and F) Expression of ZNF423 transcripts upon DNA demethylation and BMP2 stimulation. SEM cells (E) and CD34 + cord blood cells (F) were treated with 3 µM 5A2D for 24, 48, and 72 h. In SEM cells, additional BMP2 treatment was performed 6 h before lysis. Relative fold induction was measured by qPCR (2 −ΔΔCt ) using a ZNF423 isoform-specific primer design in SEM cells. mRNA levels were normalized to B2M and solvent control. Error bars represent SD out of three technical replicates. Significance is calculated using 2 −ΔCt values by Student’s t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; n.s., not significant). Data were reproduced in three independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: Aberrant ZNF423 impedes B cell differentiation and is linked to adverse outcome of ETV6-RUNX1 negative B precursor acute lymphoblastic leukemia

    doi: 10.1084/jem.20130497

    Figure Lengend Snippet: ZNF423 expression is regulated by DNA methylation. (A) Transactivation of ZNF423 isoform-specific promoters in dependence of central CGI. Reporter gene assay using ZNF423 promoters (α and β) with or without CGI after transfection in 293T cells. Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars represent SD from three technical replicates. Significance is calculated by Student’s t test, comparing indicated samples (***, P ≤ 0.001). Data were reproduced in three independent experiments. (B) Methylation map of CpGs in the central CGI at the ZNF423 locus. Genomic DNA from primary ALL ( n = 58), matched BM MNCs in complete continuous remission (MNC [CCR]; n = 58), H1, HES2 ESC lines, and normal hematopoietic cells at various stages of differentiation ( n = 42) from healthy donors were sequenced after bisulfite conversion. Each row represents one cytosine in a CG dinucleotide of the analyzed sequence. For cell lineage abbreviations, refer to Figure 1 . Significance is calculated by Student’s t test, comparing primary ALL to immunologically characterized control samples (in brackets; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001). Color code represents degree of methylation in percent. White boxes indicate no sequencing data available. (C) ZNF423 promoter activity in dependence of CPG mutational status. Disruption of CpGs was performed by site-directed mutagenesis as indicated by red marks. Combined deletion mutant (pCpGL-CGI-del_comb_α-prom) represents deletions at CGI positions 102, 183, 220, 250. Wild-type and mutated plasmids were treated with DNA-methylase M.SssI and transfected into 293T. Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars represent SD from three technical replicates. Significance is calculated by Student’s t test, comparing M.SssI-treated wild-type and mutated samples (***, P ≤ 0.001). Data were reproduced in three independent experiments. (D) DNA methylation pattern of central CGI. Diagram was created with BiQ Analyzer software from Max-Planck-Institute of Informatics. Drawing is to scale. White lollipop, unmethylated; black lollipop, methylated. Marked CpGs refer to heatmap in Fig. 4 B . (E and F) Expression of ZNF423 transcripts upon DNA demethylation and BMP2 stimulation. SEM cells (E) and CD34 + cord blood cells (F) were treated with 3 µM 5A2D for 24, 48, and 72 h. In SEM cells, additional BMP2 treatment was performed 6 h before lysis. Relative fold induction was measured by qPCR (2 −ΔΔCt ) using a ZNF423 isoform-specific primer design in SEM cells. mRNA levels were normalized to B2M and solvent control. Error bars represent SD out of three technical replicates. Significance is calculated using 2 −ΔCt values by Student’s t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; n.s., not significant). Data were reproduced in three independent experiments.

    Article Snippet: Genomic DNA was isolated from initial ALL samples and matched MNC from remission BM using the QIAGEN DNA Blood Mini kit following the manufacturer’s instructions.

    Techniques: Expressing, DNA Methylation Assay, Reporter Gene Assay, Transfection, Luciferase, Activity Assay, Methylation, Sequencing, Mutagenesis, Software, Lysis, Real-time Polymerase Chain Reaction

    Amplified pfmdr1 gene products after nested PCR. Standard DNA extraction was carried out using the QIAamp DNA mini blood kit (Qiagen, Hilden, Germany). DNA extraction by microwave irradiation was performed using a microwave oven (MDA, model number: MW17M70G-AU, 230 V, 50 HZ, operated at 800 W). 1 μl of condensed droplets after microwave treatment were utilized for the PCR procedures. First lane: DNA ladder; NC: Negative Control; PC1 and PC2: Standard extraction from archived blood sample and pfmdr1 amplicons at expected sizes; PC3 and PC4: Standard extraction from 3D7 P. falciparum parasites in culture and pfmdr1 amplicons at expected sizes; ME1 and ME2: Microwave based extraction from archived blood sample and pfmdr1 amplicons at expected sizes; ME3 and ME4: Microwave based extraction in 3D7 culture parasites and pfmdr1 amplicons at expected sizes; ME5: Microwave based DNA extraction from fresh blood sample and pfmdr1 amplicons at expected sizes.

    Journal: Malaria Journal

    Article Title: A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification

    doi: 10.1186/1475-2875-13-454

    Figure Lengend Snippet: Amplified pfmdr1 gene products after nested PCR. Standard DNA extraction was carried out using the QIAamp DNA mini blood kit (Qiagen, Hilden, Germany). DNA extraction by microwave irradiation was performed using a microwave oven (MDA, model number: MW17M70G-AU, 230 V, 50 HZ, operated at 800 W). 1 μl of condensed droplets after microwave treatment were utilized for the PCR procedures. First lane: DNA ladder; NC: Negative Control; PC1 and PC2: Standard extraction from archived blood sample and pfmdr1 amplicons at expected sizes; PC3 and PC4: Standard extraction from 3D7 P. falciparum parasites in culture and pfmdr1 amplicons at expected sizes; ME1 and ME2: Microwave based extraction from archived blood sample and pfmdr1 amplicons at expected sizes; ME3 and ME4: Microwave based extraction in 3D7 culture parasites and pfmdr1 amplicons at expected sizes; ME5: Microwave based DNA extraction from fresh blood sample and pfmdr1 amplicons at expected sizes.

    Article Snippet: DNA extraction: microwave irradiation First, DNA was extracted from whole blood samples as well as from the cultured parasites of the dilution series using the conventional QIAamp DNA mini blood kit based extraction procedure (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Techniques: Amplification, Nested PCR, DNA Extraction, Irradiation, Multiple Displacement Amplification, Polymerase Chain Reaction, Negative Control

    Control Chelex ® 100 resin-based protocol versus QIAamp DNA Blood Mini Kit for the extraction of gDNA from DBS. QIAamp DNA Blood Mini Kit was performed with a first (Ex 1, El 1) and second elution (Ex 1, El 2). Following this, the DBS was processed through the kit a second time (Ex 2, El 1 and Ex 2, El 2). The total Qiagen yield was calculated by adding together the DNA yield from both extractions and both elutions. Replicates of five were conducted for each condition in a single experiment. Data are represented as mean efficiency ± standard error.

    Journal: Biology Methods & Protocols

    Article Title: Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots

    doi: 10.1093/biomethods/bpaa009

    Figure Lengend Snippet: Control Chelex ® 100 resin-based protocol versus QIAamp DNA Blood Mini Kit for the extraction of gDNA from DBS. QIAamp DNA Blood Mini Kit was performed with a first (Ex 1, El 1) and second elution (Ex 1, El 2). Following this, the DBS was processed through the kit a second time (Ex 2, El 1 and Ex 2, El 2). The total Qiagen yield was calculated by adding together the DNA yield from both extractions and both elutions. Replicates of five were conducted for each condition in a single experiment. Data are represented as mean efficiency ± standard error.

    Article Snippet: The control Chelex® protocol yielded 590% more DNA than the QIAamp® DNA Blood Mini Kit .

    Techniques: