qiaamp dna blood mini kit (Qiagen)
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QIAamp DNA Blood Mini Kit
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For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
Catalog Number:
51104
Price:
157
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QIAamp DNA Blood Mini Kit
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Qiagen
qiaamp dna blood mini kit
For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
https://www.bioz.com/result/qiaamp dna blood mini kit/product/Qiagen
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
https://www.bioz.com/result/qiaamp dna blood mini kit/product/Qiagen
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
qiaamp dna blood mini kit - by Bioz Stars,
2021-03
97/100 stars
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DNA Extraction:Article Title: A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification Article Snippet: Equal volumes of these dilution series were further used for genomic DNA extraction by microwave irradiation and, subsequently, for standard PCR and tailored LAMP assays. .. DNA extraction: microwave irradiation First, DNA was extracted from whole blood samples as well as from the cultured parasites of the dilution series using the Irradiation:Article Title: A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification Article Snippet: Equal volumes of these dilution series were further used for genomic DNA extraction by microwave irradiation and, subsequently, for standard PCR and tailored LAMP assays. .. DNA extraction: microwave irradiation First, DNA was extracted from whole blood samples as well as from the cultured parasites of the dilution series using the Cell Culture:Article Title: A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification Article Snippet: Equal volumes of these dilution series were further used for genomic DNA extraction by microwave irradiation and, subsequently, for standard PCR and tailored LAMP assays. .. DNA extraction: microwave irradiation First, DNA was extracted from whole blood samples as well as from the cultured parasites of the dilution series using the other:Article Title: Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots Article Snippet: The control Chelex® protocol yielded 590% more DNA than the QIAamp® DNA Blood Mini Kit . Isolation:Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood Article Snippet: Two hundred microliters of Tris-EDTA (10 mM Tris-Cl and 1 mM EDTA, pH 8.0) was added to the tube containing the membrane and incubated at room temperature for 2 h with occasional finger tapping ( , Step 13) to elute the membrane-bound gDNA. .. Among the various commonly available commercial kits for gDNA isolation, the kit from Qiagen has been shown to have columns with superior binding capability., Hence, the Article Title: Aberrant ZNF423 impedes B cell differentiation and is linked to adverse outcome of ETV6-RUNX1 negative B precursor acute lymphoblastic leukemia Article Snippet: .. Genomic DNA was isolated from initial ALL samples and matched MNC from remission BM using the QIAGEN Binding Assay:Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood Article Snippet: Two hundred microliters of Tris-EDTA (10 mM Tris-Cl and 1 mM EDTA, pH 8.0) was added to the tube containing the membrane and incubated at room temperature for 2 h with occasional finger tapping ( , Step 13) to elute the membrane-bound gDNA. .. Among the various commonly available commercial kits for gDNA isolation, the kit from Qiagen has been shown to have columns with superior binding capability., Hence, the Amplification:Article Title: Inflammatory Genital Infections Mitigate a Severe Genetic Bottleneck in Heterosexual Transmission of Subtype A and C HIV-1 Article Snippet: For individuals identified as antibody negative and p24 antigen positive, the estimated date of infection was determined as 22 days prior to that date (Fiebig stage II), and finally for individuals identified as viral RNA positive and antibody/p24 antigen negative, the estimated date of infection was determined as 17 days prior to this sample date (Fiebig stage I). .. End-Point Dilution, Single Genome Amplification of HIV-1 env Genes For amplification of proviral HIV-1 env genes, genomic DNA was extracted from uncultured peripheral blood mononuclear cells (PBMC) using the Qiagen Article Title: JAK2V617F Allele Burden Measurement in Peripheral Blood of Iranian Patients with Myeloproliferative Neoplasms and Effect of Hydroxyurea on JAK2V617F Allele Burden Article Snippet: The patients were selected from Hematology-Oncology and BMT Research Center at Shariati and Imam Khomeini Hospital affiliated with Tehran University of Medical Science. .. The study was approved by our institutional review board and written informed consent was obtained from all patients. (Ethical code: ir.tums.horcsct.1394.103.7) JAK2 V617F screening by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) Genomic DNA was prepared from leukocytes using the Two Tailed Test:Article Title: Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots Article Snippet: Total gEq recovered for all conditions was calculated as the concentration of the sample by beta-globin qPCR multiplied by the final elution volume. .. We compared the mean efficiency of the Chelex® control method to the Mutagenesis:Article Title: JAK2V617F Allele Burden Measurement in Peripheral Blood of Iranian Patients with Myeloproliferative Neoplasms and Effect of Hydroxyurea on JAK2V617F Allele Burden Article Snippet: The patients were selected from Hematology-Oncology and BMT Research Center at Shariati and Imam Khomeini Hospital affiliated with Tehran University of Medical Science. .. The study was approved by our institutional review board and written informed consent was obtained from all patients. (Ethical code: ir.tums.horcsct.1394.103.7) JAK2 V617F screening by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) Genomic DNA was prepared from leukocytes using the |
![ZNF423 expression is regulated by DNA methylation. (A) Transactivation of ZNF423 isoform-specific promoters in dependence of central CGI. Reporter gene assay using ZNF423 promoters (α and β) with or without CGI after transfection in 293T cells. Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars represent SD from three technical replicates. Significance is calculated by Student’s t test, comparing indicated samples (***, P ≤ 0.001). Data were reproduced in three independent experiments. (B) Methylation map of CpGs in the central CGI at the ZNF423 locus. Genomic DNA from primary ALL ( n = 58), matched BM MNCs in complete continuous remission (MNC [CCR]; n = 58), H1, HES2 ESC lines, and normal hematopoietic cells at various stages of differentiation ( n = 42) from healthy donors were sequenced after bisulfite conversion. Each row represents one cytosine in a CG dinucleotide of the analyzed sequence. For cell lineage abbreviations, refer to Figure 1 . Significance is calculated by Student’s t test, comparing primary ALL to immunologically characterized control samples (in brackets; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001). Color code represents degree of methylation in percent. White boxes indicate no sequencing data available. (C) ZNF423 promoter activity in dependence of CPG mutational status. Disruption of CpGs was performed by site-directed mutagenesis as indicated by red marks. Combined deletion mutant (pCpGL-CGI-del_comb_α-prom) represents deletions at CGI positions 102, 183, 220, 250. Wild-type and mutated plasmids were treated with DNA-methylase M.SssI and transfected into 293T. Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars represent SD from three technical replicates. Significance is calculated by Student’s t test, comparing M.SssI-treated wild-type and mutated samples (***, P ≤ 0.001). Data were reproduced in three independent experiments. (D) DNA methylation pattern of central CGI. Diagram was created with BiQ Analyzer software from Max-Planck-Institute of Informatics. Drawing is to scale. White lollipop, unmethylated; black lollipop, methylated. Marked CpGs refer to heatmap in Fig. 4 B . (E and F) Expression of ZNF423 transcripts upon DNA demethylation and BMP2 stimulation. SEM cells (E) and CD34 + cord blood cells (F) were treated with 3 µM 5A2D for 24, 48, and 72 h. In SEM cells, additional BMP2 treatment was performed 6 h before lysis. Relative fold induction was measured by qPCR (2 −ΔΔCt ) using a ZNF423 isoform-specific primer design in SEM cells. mRNA levels were normalized to B2M and solvent control. Error bars represent SD out of three technical replicates. Significance is calculated using 2 −ΔCt values by Student’s t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; n.s., not significant). Data were reproduced in three independent experiments.](https://storage.googleapis.com/bioz_article_images/PMC3804944/JEM_20130497_Fig4.jpg)
