q5 hot star high fidelity dna polymerase  (New England Biolabs)


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    Name:
    Q5 Hot Start High Fidelity DNA Polymerase
    Description:
    Q5 Hot Start High Fidelity DNA Polymerase 500 units
    Catalog Number:
    m0493l
    Price:
    543
    Size:
    500 units
    Category:
    Thermostable DNA Polymerases
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    Structured Review

    New England Biolabs q5 hot star high fidelity dna polymerase
    Q5 Hot Start High Fidelity DNA Polymerase
    Q5 Hot Start High Fidelity DNA Polymerase 500 units
    https://www.bioz.com/result/q5 hot star high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    q5 hot star high fidelity dna polymerase - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins"

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    Journal: BMC Biotechnology

    doi: 10.1186/s12896-016-0316-3

    Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I
    Figure Legend Snippet: Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Techniques Used: Negative Control

    2) Product Images from "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins"

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    Journal: BMC Biotechnology

    doi: 10.1186/s12896-016-0316-3

    Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I
    Figure Legend Snippet: Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Techniques Used: Negative Control

    3) Product Images from "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins"

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    Journal: BMC Biotechnology

    doi: 10.1186/s12896-016-0316-3

    Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I
    Figure Legend Snippet: Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Techniques Used: Negative Control

    4) Product Images from "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins"

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    Journal: BMC Biotechnology

    doi: 10.1186/s12896-016-0316-3

    Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I
    Figure Legend Snippet: Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Techniques Used: Negative Control

    Related Articles

    Clone Assay:

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error
    Article Snippet: PCR products were purified by EasyPure PCR Purification Kit (Transgen Biotech, China), and then cloned using pEASY-T1 Cloning Kit (Transgen Biotech, China). .. Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ].

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Article Title: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
    Article Snippet: Amplification was made using a Q5 Hot-Start High-Fidelity DNA Polymerase (NEB ) with 35 cycles of Denaturation (98 °C, 20 s), Annealing (65 °C, 15 s) and Extension (72 °C, 30 s). .. The final PCR product was cloned into a p15 plasmid flanked by a pLacI constitutive promoter and the rrnB1 terminator.

    Amplification:

    Article Title: Genome-wide mapping of alternative splicing in Arabidopsis thaliana
    Article Snippet: .. The fractionated libraries were PCR amplified using Phusion Hot Start High-Fidelity DNA polymerase (NEB) and the following PCR protocol: 30 sec at 98°C, then 10 sec at 98°C, 30 sec at 65°C, and 30 sec at 72°C for 18 cycles, followed by a 10-min extension step at 72°C. .. Prior to cluster generation, DNA was diluted to a final concentration of 5–10 pM to generate 25,000 to 40,000 clusters per tile of the flow cell.

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ). .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Article Title: A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure
    Article Snippet: The sgRNAs were amplified, and adaptors were attached in a single PCR step. .. PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using the following forward primer: aatgatacggcgaccaccgaGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNgcacaaaaggaaactcaccct and reverse primer: caagcagaagacggcatacgaCGACTCGGTGCCACTTTTTC, which includes necessary adaptor and indexing sequences.

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error
    Article Snippet: We amplified the full length SSU rDNA of B. americanum with universal primers [ ] using PfuTurbo DNA polymerase (Agilent Technologies, USA). .. Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ].

    Article Title: Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations. Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, sequencing ... Unsuccessful PCR reactions were re‐run using the Q5 Host Start High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, Massachusetts).

    Article Title: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
    Article Snippet: .. Amplification was made using a Q5 Hot-Start High-Fidelity DNA Polymerase (NEB ) with 35 cycles of Denaturation (98 °C, 20 s), Annealing (65 °C, 15 s) and Extension (72 °C, 30 s). .. Secondly, the product band was gel purified with the MinElute Gel Extraction Kit (QIAGEN ) and an endonuclease correction step was performed with the Surveyor® Mutation Detection Kit (IDT) as previously described at [ ].

    Article Title: Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein
    Article Snippet: .. The wild-type Rep 78 (Rep 78 WT) sequence was PCR amplified using pAAV-DJ Vector DNA (Cell Biolabs, Inc., San Diego, CA) as a template and Q5 and hot start high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). .. The hCas9 plasmid (Addgene; plasmid # 41815), the hCas_D10A plasmid (Addgene; plasmid # 41816), and the gRNA_AAVS1-T2 plasmid (Addgene; plasmid # 41818) were gifts from George Church Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

    Synthesized:

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites. .. Following transformation, colonies were screened by PCR for the desired sequence, and positive clones were confirmed by sequencing by Genewiz.

    Article Title: Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein
    Article Snippet: DNA sequences encoding Rep 78-III and the ZFNSh-L and ZFNSh-R proteins were synthesized by GenScript (Piscataway, NJ) based on the sequences described by Sitaraman et al. , Hockemeyer et al. , and Guo et al. , and were subcloned into pENTR2B (Invitrogen, Carlsbad, CA). .. The wild-type Rep 78 (Rep 78 WT) sequence was PCR amplified using pAAV-DJ Vector DNA (Cell Biolabs, Inc., San Diego, CA) as a template and Q5 and hot start high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA).

    Construct:

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: Point mutations were introduced into the Envs by site-directed mutagenesis with a QuikChange site-directed mutagenesis kit (Stratagene, Inc.), and chimeric Envs were generated by blunt end ligation or Gibson assembly (New England BioLabs, Inc. [NEB]), which utilizes overlapping regions to ligate various DNA fragments into singular constructs. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Article Title: Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein
    Article Snippet: Paragraph title: Plasmid constructs ... The wild-type Rep 78 (Rep 78 WT) sequence was PCR amplified using pAAV-DJ Vector DNA (Cell Biolabs, Inc., San Diego, CA) as a template and Q5 and hot start high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA).

    Incubation:

    Article Title: Genome-wide mapping of alternative splicing in Arabidopsis thaliana
    Article Snippet: To ligate Illumina adapters, 10 μL of cDNA from the prior step was mixed with 5 μL of 5× T4 DNA ligase buffer, 6 μL of adapter oligo mix, 4 μL of T4 DNA ligase (NEB), and incubated for 15 min at 25°C. .. The fractionated libraries were PCR amplified using Phusion Hot Start High-Fidelity DNA polymerase (NEB) and the following PCR protocol: 30 sec at 98°C, then 10 sec at 98°C, 30 sec at 65°C, and 30 sec at 72°C for 18 cycles, followed by a 10-min extension step at 72°C.

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Pre-selection libraries were also separately incubated with 2 U of BspMI (NEB) in NEBuffer 3.1 for 1 h at 37 °C. .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Expressing:

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: The MW965.26 and codon-optimized consensus C HIV-1 Envs were obtained from the NIH AIDS Reagent Program and expressed in the pcDNA3.1 mammalian expression vector (Thermo Fisher Scientific); for the MW965.26 Env, rev was coexpressed in the plasmid. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Transformation Assay:

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Flow Cytometry:

    Article Title: Genome-wide mapping of alternative splicing in Arabidopsis thaliana
    Article Snippet: The fractionated libraries were PCR amplified using Phusion Hot Start High-Fidelity DNA polymerase (NEB) and the following PCR protocol: 30 sec at 98°C, then 10 sec at 98°C, 30 sec at 65°C, and 30 sec at 72°C for 18 cycles, followed by a 10-min extension step at 72°C. .. Prior to cluster generation, DNA was diluted to a final concentration of 5–10 pM to generate 25,000 to 40,000 clusters per tile of the flow cell.

    Ligation:

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: Point mutations were introduced into the Envs by site-directed mutagenesis with a QuikChange site-directed mutagenesis kit (Stratagene, Inc.), and chimeric Envs were generated by blunt end ligation or Gibson assembly (New England BioLabs, Inc. [NEB]), which utilizes overlapping regions to ligate various DNA fragments into singular constructs. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Cell Culture:

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error
    Article Snippet: One clone was picked randomly and cultured in LB broth medium for 15 h to extract the plasmid using Sanprep Plasmid Miniprep Kit (Sangon Biotech, Shanghai). .. Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ].

    Generated:

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites. .. Following transformation, colonies were screened by PCR for the desired sequence, and positive clones were confirmed by sequencing by Genewiz.

    other:

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins
    Article Snippet: Finally, extension was performed at 70 °C for 3 s for KOD Hot Start DNA polymerase, 72 °C for 30 s for Pfu Turbo and Taq, and 72 °C for 15 s for Q5® Hot Star High Fidelity DNA polymerase.

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins
    Article Snippet: Annealing occurred at 60 °C for 10 s for KOD Hot Start DNA polymerase and 60 °C for 30 s for Q5® Hot Star High Fidelity DNA polymerase, Pfu Turbo and Taq.

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins
    Article Snippet: Denaturation was performed at 95 °C for 30 s for Pfu Turbo and Taq, 95 °C for 16 s for KOD Hot Start DNA polymerase and 98 °C for 10 s for Q5® Hot Star High Fidelity DNA polymerase.

    Polymerase Chain Reaction:

    Article Title: Genome-wide mapping of alternative splicing in Arabidopsis thaliana
    Article Snippet: .. The fractionated libraries were PCR amplified using Phusion Hot Start High-Fidelity DNA polymerase (NEB) and the following PCR protocol: 30 sec at 98°C, then 10 sec at 98°C, 30 sec at 65°C, and 30 sec at 72°C for 18 cycles, followed by a 10-min extension step at 72°C. .. Prior to cluster generation, DNA was diluted to a final concentration of 5–10 pM to generate 25,000 to 40,000 clusters per tile of the flow cell.

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ). .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Article Title: A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure
    Article Snippet: .. PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using the following forward primer: aatgatacggcgaccaccgaGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNgcacaaaaggaaactcaccct and reverse primer: caagcagaagacggcatacgaCGACTCGGTGCCACTTTTTC, which includes necessary adaptor and indexing sequences. .. “N” refers to the variable index sequence.

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error
    Article Snippet: PCR products were purified by EasyPure PCR Purification Kit (Transgen Biotech, China), and then cloned using pEASY-T1 Cloning Kit (Transgen Biotech, China). .. Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ].

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins
    Article Snippet: Paragraph title: Optimization of PCR conditions for successful gene synthesis protocol ... Four DNA polymerases were selected for these studies: KOD Hot Start DNA polymerase (EMD-Millipore), Q5® Hot Star High Fidelity DNA polymerase (New England Biolabs), Pfu Turbo DNA polymerase (Agilent Technologies) and Taq DNA polymerase (Sigma-Aldrich).

    Article Title: Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations. Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations
    Article Snippet: .. Unsuccessful PCR reactions were re‐run using the Q5 Host Start High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, Massachusetts). .. PCR was done in 25 μl consisting of 5.0 μl of 5× Q5 Reaction Buffer, 0.5 μl of 10 mM dNTP Mix (Quantabio, Beverly, Massachusetts), 1.25 μl of each 10 μM primer, 0.25 μl of Q5 High‐Fidelity DNA Polymerase, 12.75 μl of H2 O and 4.0 μl of template DNA.

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Article Title: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
    Article Snippet: Amplification was made using a Q5 Hot-Start High-Fidelity DNA Polymerase (NEB ) with 35 cycles of Denaturation (98 °C, 20 s), Annealing (65 °C, 15 s) and Extension (72 °C, 30 s). .. Finally, a second PCR step was performed to amplify the Surveyor treated template with the gene’s end primers (PcaV DE WT 1F and PcaV DE WT 10R).

    Article Title: Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein
    Article Snippet: .. The wild-type Rep 78 (Rep 78 WT) sequence was PCR amplified using pAAV-DJ Vector DNA (Cell Biolabs, Inc., San Diego, CA) as a template and Q5 and hot start high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). .. The hCas9 plasmid (Addgene; plasmid # 41815), the hCas_D10A plasmid (Addgene; plasmid # 41816), and the gRNA_AAVS1-T2 plasmid (Addgene; plasmid # 41818) were gifts from George Church Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

    DNA Extraction:

    Article Title: Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations. Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, sequencing ... Unsuccessful PCR reactions were re‐run using the Q5 Host Start High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, Massachusetts).

    Mutagenesis:

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: Paragraph title: Generation of chimeric and mutant Envs. ... In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Article Title: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
    Article Snippet: Amplification was made using a Q5 Hot-Start High-Fidelity DNA Polymerase (NEB ) with 35 cycles of Denaturation (98 °C, 20 s), Annealing (65 °C, 15 s) and Extension (72 °C, 30 s). .. Secondly, the product band was gel purified with the MinElute Gel Extraction Kit (QIAGEN ) and an endonuclease correction step was performed with the Surveyor® Mutation Detection Kit (IDT) as previously described at [ ].

    Isolation:

    Article Title: A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure
    Article Snippet: The amount of 1.1 μg of undigested genomic DNA was used per 50 μL PCR reaction, and sufficient reactions were performed to include all isolated genomic DNA. .. PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using the following forward primer: aatgatacggcgaccaccgaGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNgcacaaaaggaaactcaccct and reverse primer: caagcagaagacggcatacgaCGACTCGGTGCCACTTTTTC, which includes necessary adaptor and indexing sequences.

    Size-exclusion Chromatography:

    Article Title: Genome-wide mapping of alternative splicing in Arabidopsis thaliana
    Article Snippet: .. The fractionated libraries were PCR amplified using Phusion Hot Start High-Fidelity DNA polymerase (NEB) and the following PCR protocol: 30 sec at 98°C, then 10 sec at 98°C, 30 sec at 65°C, and 30 sec at 72°C for 18 cycles, followed by a 10-min extension step at 72°C. .. Prior to cluster generation, DNA was diluted to a final concentration of 5–10 pM to generate 25,000 to 40,000 clusters per tile of the flow cell.

    Purification:

    Article Title: Genome-wide mapping of alternative splicing in Arabidopsis thaliana
    Article Snippet: DNA was purified using a QIAquick MinElute PCR Purification kit. cDNA was size-fractionated (typically with average fragment length of 170 base pairs [bp]) on 3.5% (w/v) NuSieve GTG agarose. .. The fractionated libraries were PCR amplified using Phusion Hot Start High-Fidelity DNA polymerase (NEB) and the following PCR protocol: 30 sec at 98°C, then 10 sec at 98°C, 30 sec at 65°C, and 30 sec at 72°C for 18 cycles, followed by a 10-min extension step at 72°C.

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ). .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error
    Article Snippet: PCR products were purified by EasyPure PCR Purification Kit (Transgen Biotech, China), and then cloned using pEASY-T1 Cloning Kit (Transgen Biotech, China). .. Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ].

    Article Title: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
    Article Snippet: Amplification was made using a Q5 Hot-Start High-Fidelity DNA Polymerase (NEB ) with 35 cycles of Denaturation (98 °C, 20 s), Annealing (65 °C, 15 s) and Extension (72 °C, 30 s). .. Secondly, the product band was gel purified with the MinElute Gel Extraction Kit (QIAGEN ) and an endonuclease correction step was performed with the Surveyor® Mutation Detection Kit (IDT) as previously described at [ ].

    Sequencing:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ). .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Article Title: A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure
    Article Snippet: Paragraph title: DNA preparation and sequencing ... PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using the following forward primer: aatgatacggcgaccaccgaGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNgcacaaaaggaaactcaccct and reverse primer: caagcagaagacggcatacgaCGACTCGGTGCCACTTTTTC, which includes necessary adaptor and indexing sequences.

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error
    Article Snippet: Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ]. .. The SSU rDNA in the plasmid was sequenced bidirectionally both in GENEWIZ Incorporated Company (Beijing, China) and Shanghai Sunny Biotechnology Company (Shanghai, China) to reduce the impact of errors caused by sequencing.

    Article Title: Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations. Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, sequencing ... Unsuccessful PCR reactions were re‐run using the Q5 Host Start High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, Massachusetts).

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Article Title: Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein
    Article Snippet: .. The wild-type Rep 78 (Rep 78 WT) sequence was PCR amplified using pAAV-DJ Vector DNA (Cell Biolabs, Inc., San Diego, CA) as a template and Q5 and hot start high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). .. The hCas9 plasmid (Addgene; plasmid # 41815), the hCas_D10A plasmid (Addgene; plasmid # 41816), and the gRNA_AAVS1-T2 plasmid (Addgene; plasmid # 41818) were gifts from George Church Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

    FACS:

    Article Title: A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure
    Article Snippet: DNA preparation and sequencing Cells collected by FACS were centrifuged, and pellets were frozen at −20 °C until processing. .. PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using the following forward primer: aatgatacggcgaccaccgaGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNgcacaaaaggaaactcaccct and reverse primer: caagcagaagacggcatacgaCGACTCGGTGCCACTTTTTC, which includes necessary adaptor and indexing sequences.

    Plasmid Preparation:

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error
    Article Snippet: .. Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ]. ..

    Article Title: Identification of Novel Structural Determinants in MW965 Env That Regulate the Neutralization Phenotype and Conformational Masking Potential of Primary HIV-1 Isolates
    Article Snippet: The MW965.26 and codon-optimized consensus C HIV-1 Envs were obtained from the NIH AIDS Reagent Program and expressed in the pcDNA3.1 mammalian expression vector (Thermo Fisher Scientific); for the MW965.26 Env, rev was coexpressed in the plasmid. .. In some experiments, DNA fragments were generated by Phusion Hot Start High-Fidelity DNA polymerase (NEB) or synthesized as gBlock gene fragments (Integrated DNA Technologies, Inc. [IDT]) and exchanged into Env at specific restriction enzyme sites.

    Article Title: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
    Article Snippet: Amplification was made using a Q5 Hot-Start High-Fidelity DNA Polymerase (NEB ) with 35 cycles of Denaturation (98 °C, 20 s), Annealing (65 °C, 15 s) and Extension (72 °C, 30 s). .. The final PCR product was cloned into a p15 plasmid flanked by a pLacI constitutive promoter and the rrnB1 terminator.

    Article Title: Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein
    Article Snippet: .. The wild-type Rep 78 (Rep 78 WT) sequence was PCR amplified using pAAV-DJ Vector DNA (Cell Biolabs, Inc., San Diego, CA) as a template and Q5 and hot start high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). .. The hCas9 plasmid (Addgene; plasmid # 41815), the hCas_D10A plasmid (Addgene; plasmid # 41816), and the gRNA_AAVS1-T2 plasmid (Addgene; plasmid # 41818) were gifts from George Church Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

    Selection:

    Article Title: A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure
    Article Snippet: PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using the following forward primer: aatgatacggcgaccaccgaGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNgcacaaaaggaaactcaccct and reverse primer: caagcagaagacggcatacgaCGACTCGGTGCCACTTTTTC, which includes necessary adaptor and indexing sequences. .. The resulting PCR products from multiple reactions were pooled, and unincorporated primers were removed using the GeneRead Size Selection Kit (Qiagen).

    In Vitro:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Paragraph title: In vitro high-throughput specificity profiling ... Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Concentration Assay:

    Article Title: Genome-wide mapping of alternative splicing in Arabidopsis thaliana
    Article Snippet: The fractionated libraries were PCR amplified using Phusion Hot Start High-Fidelity DNA polymerase (NEB) and the following PCR protocol: 30 sec at 98°C, then 10 sec at 98°C, 30 sec at 65°C, and 30 sec at 72°C for 18 cycles, followed by a 10-min extension step at 72°C. .. Prior to cluster generation, DNA was diluted to a final concentration of 5–10 pM to generate 25,000 to 40,000 clusters per tile of the flow cell.

    Article Title: Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations. Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations
    Article Snippet: Unsuccessful PCR reactions were re‐run using the Q5 Host Start High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, Massachusetts). .. The optimal annealing temperature (Ta) was calculated for every primer combination using the New England BioLabs online Tm Calculator tool (tmcalculator.neb.com/) selecting “Q5” as product group and “Q5 Hot Start High‐Fidelity DNA Polymerase” as polymerase/kit, and with 500 mM for primer concentration.

    High Throughput Screening Assay:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Paragraph title: In vitro high-throughput specificity profiling ... Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Gel Extraction:

    Article Title: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
    Article Snippet: Amplification was made using a Q5 Hot-Start High-Fidelity DNA Polymerase (NEB ) with 35 cycles of Denaturation (98 °C, 20 s), Annealing (65 °C, 15 s) and Extension (72 °C, 30 s). .. Secondly, the product band was gel purified with the MinElute Gel Extraction Kit (QIAGEN ) and an endonuclease correction step was performed with the Surveyor® Mutation Detection Kit (IDT) as previously described at [ ].

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    New England Biolabs q5 hot star high fidelity dna polymerase
    Performance of four thermostable <t>DNA</t> polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: <t>Q5</t> Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I
    Q5 Hot Star High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 hot star high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    q5 hot star high fidelity dna polymerase - by Bioz Stars, 2020-04
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    Q5U Hot Start High Fidelity DNA Polymerase is a modified version of Q5 High Fidelity DNA Polymerase which efficiently incorporates dUTP and amplifies uracil containing DNA templates This feature is
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    Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Journal: BMC Biotechnology

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    doi: 10.1186/s12896-016-0316-3

    Figure Lengend Snippet: Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Article Snippet: Finally, extension was performed at 70 °C for 3 s for KOD Hot Start DNA polymerase, 72 °C for 30 s for Pfu Turbo and Taq, and 72 °C for 15 s for Q5® Hot Star High Fidelity DNA polymerase.

    Techniques: Negative Control