q5 high fidelity dna polymerase  (New England Biolabs)


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    Name:
    Q5 High Fidelity DNA Polymerase
    Description:
    Q5 High Fidelity DNA Polymerase 500 units
    Catalog Number:
    m0491l
    Price:
    441
    Size:
    500 units
    Category:
    Thermostable DNA Polymerases
    Buy from Supplier


    Structured Review

    New England Biolabs q5 high fidelity dna polymerase
    Q5 High Fidelity DNA Polymerase
    Q5 High Fidelity DNA Polymerase 500 units
    https://www.bioz.com/result/q5 high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 1208 article reviews
    Price from $9.99 to $1999.99
    q5 high fidelity dna polymerase - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer"

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.12305

    Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.
    Figure Legend Snippet: Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Techniques Used: Mutagenesis, Amplification

    2) Product Images from "Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer"

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.12305

    Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.
    Figure Legend Snippet: Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Techniques Used: Mutagenesis, Amplification

    3) Product Images from "Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer"

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.12305

    Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.
    Figure Legend Snippet: Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Techniques Used: Mutagenesis, Amplification

    Related Articles

    Multiplex Assay:

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer
    Article Snippet: .. To increase the sensitivity of the analysis, the remaining 75 μL serum DNA was subjected to 12 cycles of PCR pre‐amplification with Q5 High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) using a multiplex PIK3CA primer mix. ..

    Amplification:

    Article Title: In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR
    Article Snippet: .. Certain DNA sequences, such as the origin of replication, promoters, and polyadenylation sequences can be amplified extensively error-free with Q5 DNA polymerase. .. Considering that all the DNA fragments in pDSADE were PCR amplified and in vivo assembled through multiple rounds of cloning following the paths: pcDNA3.1/Hygro (+) → pcDNA3 Kan → pDcEG → pDSADE and pET28a-Ec.coaA + pET28a-Ec.coaD + pETite N-His SUMO → pDSA → pDSADE, it is remarkable that a randomly selected 16.1 kb target pDSADE has the exact pre-defined sequence without a single mutation.

    Mutagenesis:

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer
    Article Snippet: .. From the nonmutated alleles, the level of blank (LoB) for each of the four PIK3CA mutation assays was determined using pre‐amplified cell‐free plasma DNA and the Q5 High‐Fidelity DNA Polymerase (New England BioLabs). ..

    Generated:

    Article Title: CRISPR/Cas9 Editing of the Bacillus subtilis Genome
    Article Snippet: .. The PCR program used to linearize pPB41 is: PCR amplify CRISPR/Cas9 using plasmid generated in step B1 ( ) Amplify plasmid generated in step B1 (pPB43 in the example) via PCR using Q5 DNA polymerase (NEB) and primers oPEB232 and oPEB234. .. Perform 1% agarose gel electrophoresis in 1x TAE buffer (see Recipes) followed by gel extraction.

    other:

    Article Title: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
    Article Snippet: We then added 6 μL of 1:10 diluted ERCC spike-in RNA per 10 μg of total RNA.

    CRISPR:

    Article Title: CRISPR/Cas9 Editing of the Bacillus subtilis Genome
    Article Snippet: .. The PCR program used to linearize pPB41 is: PCR amplify CRISPR/Cas9 using plasmid generated in step B1 ( ) Amplify plasmid generated in step B1 (pPB43 in the example) via PCR using Q5 DNA polymerase (NEB) and primers oPEB232 and oPEB234. .. Perform 1% agarose gel electrophoresis in 1x TAE buffer (see Recipes) followed by gel extraction.

    Polymerase Chain Reaction:

    Article Title: In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR
    Article Snippet: .. Conclusions A 2-consecutive PCR procedure using Q5 DNA polymerase has been developed to prepare high quality DNA fragments up to 12 kb and reduce template plasmid concentrations below 10 fg/μL. .. Directtransformation of a mixture of PCR DNA fragments containing overlapping ends into DH5α cells yields correctly assembled plasmids with a zero background from template plasmids.

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer
    Article Snippet: .. To increase the sensitivity of the analysis, the remaining 75 μL serum DNA was subjected to 12 cycles of PCR pre‐amplification with Q5 High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) using a multiplex PIK3CA primer mix. ..

    Article Title: CRISPR/Cas9 Editing of the Bacillus subtilis Genome
    Article Snippet: .. The PCR program used to linearize pPB41 is: PCR amplify CRISPR/Cas9 using plasmid generated in step B1 ( ) Amplify plasmid generated in step B1 (pPB43 in the example) via PCR using Q5 DNA polymerase (NEB) and primers oPEB232 and oPEB234. .. Perform 1% agarose gel electrophoresis in 1x TAE buffer (see Recipes) followed by gel extraction.

    Article Title: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye, and inhibitor-resistant high-fidelity DNA polymerase
    Article Snippet: .. Based on the lowest background signal, and reported ( ) inhibitor resistance in DNA extraction-free PCR, we chose to continue the optimization with Q5 DNA polymerase. ..

    Plasmid Preparation:

    Article Title: In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR
    Article Snippet: .. Conclusions A 2-consecutive PCR procedure using Q5 DNA polymerase has been developed to prepare high quality DNA fragments up to 12 kb and reduce template plasmid concentrations below 10 fg/μL. .. Directtransformation of a mixture of PCR DNA fragments containing overlapping ends into DH5α cells yields correctly assembled plasmids with a zero background from template plasmids.

    Article Title: CRISPR/Cas9 Editing of the Bacillus subtilis Genome
    Article Snippet: .. The PCR program used to linearize pPB41 is: PCR amplify CRISPR/Cas9 using plasmid generated in step B1 ( ) Amplify plasmid generated in step B1 (pPB43 in the example) via PCR using Q5 DNA polymerase (NEB) and primers oPEB232 and oPEB234. .. Perform 1% agarose gel electrophoresis in 1x TAE buffer (see Recipes) followed by gel extraction.

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  • 99
    New England Biolabs q5 dna high fidelity polymerase
    Deoxynucleoside diphosphate utilization by DNA polymerases. ( A ) A standard PCR with a monophosphate substrate is inhibited, whereas the presence of all four di- or triphosphate nucleotides supports DNA amplification. Thermophilic polymerases Taq, Vent (exo−), and Pfu , as well as B , Deep Vent, and <t>Q5</t> DNA polymerases, also utilize the diphosphorylated substrates. ( C and D ) Primer-extension reactions on short templates sampled at indicated time points. All reactions were performed with 100 µM dNDPs at 60 °C, except the Bsu reactions, which were performed at 37 °C. Extended sequences are shown alongside of gels and were distinct in C and D . Primer band is indicated with “–P.” Pausing appears mainly before incorporation of dA and dC and is variable among the polymerases. ( E ).
    Q5 Dna High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 dna high fidelity polymerase/product/New England Biolabs
    Average 99 stars, based on 1215 article reviews
    Price from $9.99 to $1999.99
    q5 dna high fidelity polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Deoxynucleoside diphosphate utilization by DNA polymerases. ( A ) A standard PCR with a monophosphate substrate is inhibited, whereas the presence of all four di- or triphosphate nucleotides supports DNA amplification. Thermophilic polymerases Taq, Vent (exo−), and Pfu , as well as B , Deep Vent, and Q5 DNA polymerases, also utilize the diphosphorylated substrates. ( C and D ) Primer-extension reactions on short templates sampled at indicated time points. All reactions were performed with 100 µM dNDPs at 60 °C, except the Bsu reactions, which were performed at 37 °C. Extended sequences are shown alongside of gels and were distinct in C and D . Primer band is indicated with “–P.” Pausing appears mainly before incorporation of dA and dC and is variable among the polymerases. ( E ).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: DNA synthesis from diphosphate substrates by DNA polymerases

    doi: 10.1073/pnas.1712193115

    Figure Lengend Snippet: Deoxynucleoside diphosphate utilization by DNA polymerases. ( A ) A standard PCR with a monophosphate substrate is inhibited, whereas the presence of all four di- or triphosphate nucleotides supports DNA amplification. Thermophilic polymerases Taq, Vent (exo−), and Pfu , as well as B , Deep Vent, and Q5 DNA polymerases, also utilize the diphosphorylated substrates. ( C and D ) Primer-extension reactions on short templates sampled at indicated time points. All reactions were performed with 100 µM dNDPs at 60 °C, except the Bsu reactions, which were performed at 37 °C. Extended sequences are shown alongside of gels and were distinct in C and D . Primer band is indicated with “–P.” Pausing appears mainly before incorporation of dA and dC and is variable among the polymerases. ( E ).

    Article Snippet: Taq DNA polymerase, Phusion High-Fidelity DNA polymerase, DeepVent DNA polymerase, Vent DNA polymerase, Q5 DNA High-Fidelity polymerase, Bst polymerase, Bsu DNA polymerase (large fragment), and ThermoPol Reaction Buffer [1×: 20 mM Tris⋅HCl, 10 mM (NH4 )2 SO4 , 10 mM KCl, 2 mM MgSO4 , 0.1% Triton–X–100, pH 8.8 at 25 °C] were purchased from New England Biolabs.

    Techniques: Polymerase Chain Reaction, Amplification

    Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Journal: Molecular Oncology

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer

    doi: 10.1002/1878-0261.12305

    Figure Lengend Snippet: Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Article Snippet: To increase the sensitivity of the analysis, the remaining 75 μL serum DNA was subjected to 12 cycles of PCR pre‐amplification with Q5 High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) using a multiplex PIK3CA primer mix.

    Techniques: Mutagenesis, Amplification