ins 1 3 p 2  (Echelon Biosciences)


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    Echelon Biosciences ins 1 3 p 2
    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Ins 1 3 P 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "FYVE-Dependent Endosomal Targeting of an Arrestin-Related Protein in Amoeba"

    Article Title: FYVE-Dependent Endosomal Targeting of an Arrestin-Related Protein in Amoeba

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0015249

    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Figure Legend Snippet: ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).

    Techniques Used: Overlay Assay, Binding Assay, Fluorescence, Expressing

    ins 1 3 p 2  (Echelon Biosciences)


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    Echelon Biosciences ins 1 3 p 2
    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Ins 1 3 P 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "FYVE-Dependent Endosomal Targeting of an Arrestin-Related Protein in Amoeba"

    Article Title: FYVE-Dependent Endosomal Targeting of an Arrestin-Related Protein in Amoeba

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0015249

    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Figure Legend Snippet: ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).

    Techniques Used: Overlay Assay, Binding Assay, Fluorescence, Expressing

    inositol  (Echelon Biosciences)


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    Echelon Biosciences inositol
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    inositol 1 4 5 phosphate  (Echelon Biosciences)


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    1 3 4 5 tetraphosphate  (Echelon Biosciences)


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    Echelon Biosciences 1 3 4 5 tetraphosphate
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    inositol  (Echelon Biosciences)


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    Echelon Biosciences inositol
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    inositol 1 4 5 phosphate  (Echelon Biosciences)


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    inositol  (Echelon Biosciences)


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    1 3 4 5 tetraphosphate  (Echelon Biosciences)


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    Echelon Biosciences ins 1 3 p 2
    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Ins 1 3 P 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences inositol
    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Inositol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences inositol 1 4 5 phosphate
    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Inositol 1 4 5 Phosphate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences 1 3 4 5 tetraphosphate
    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    1 3 4 5 Tetraphosphate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences inositol 1 3 4 5 tetraphosphate
    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).
    Inositol 1 3 4 5 Tetraphosphate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).

    Journal: PLoS ONE

    Article Title: FYVE-Dependent Endosomal Targeting of an Arrestin-Related Protein in Amoeba

    doi: 10.1371/journal.pone.0015249

    Figure Lengend Snippet: ( A ) Lipid overlay assay. AdcA FYVE domain was expressed as a MBP-fusion protein and tested for its binding to lipids immobilized on a membrane. MBP alone was used as a control. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PI, phosphatidylinositol; PI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate; PI5P, phosphatidylinositol 5-phosphate; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine 1-phosphate; PI3,4P 2 , phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 , phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 , phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 , phosphatidylinositol 3,4,5-trisphosphate; PA, phosphatidic acid; PS, phosphatidylserine; no, blank. ( B ) Binding of Ins(1,3)P 2 . Variations of tryptophan intrinsic fluorescence (λ ex 290 nm) of MBP-FYVE (o) and MBP (•) were measured between 300 and 400 nm in response to addition of Ins(1,3)P 2 , a soluble analog of PI(3)P. Variations were expressed as ΔF/F 0 in percent. ( C ) AdcA membrane association is pH dependent. Cells expressing AdcA GFP were broken in different buffers at different pHs and treated as in panel ( A ).

    Article Snippet: PIP strips and Ins(1,3)P 2 were purchased from Echelon Biosciences Inc. (Tebu-bio, Le-Perray-en-Yvelines, France).

    Techniques: Overlay Assay, Binding Assay, Fluorescence, Expressing