pyromark pcr kit  (Qiagen)


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    Structured Review

    Qiagen pyromark pcr kit
    Pyromark Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyromark pcr kit/product/Qiagen
    Average 99 stars, based on 480 article reviews
    Price from $9.99 to $1999.99
    pyromark pcr kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Positive Control:

    Article Title: Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons.
    Article Snippet: Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways. .. Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways.

    Methylation:

    Article Title: Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons.
    Article Snippet: Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways. .. Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways.

    Isolation:

    Article Title: Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons.
    Article Snippet: Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways. .. Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways.

    DNA Methylation Assay:

    Article Title: Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons.
    Article Snippet: Paragraph title: Allele-specific DNA methylation ... Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways.

    Polymerase Chain Reaction:

    Article Title: Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons.
    Article Snippet: .. Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways. .. Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways.

    Sequencing:

    Article Title: Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons.
    Article Snippet: Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways. .. Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways.

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    Qiagen pyromark gold q96 reagents kit
    Calibration curves generated from the predictions of the AQ module of the <t>PyroMark</t> <t>Q96</t> 2.5.8 software
    Pyromark Gold Q96 Reagents Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyromark gold q96 reagents kit/product/Qiagen
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pyromark gold q96 reagents kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Qiagen bisul methylation universal kit
    Outline of the procedure for measuring Htr2c RNA editing. Total RNA was extracted from the homogenized amygdala of the mice brain using the TRI zol method. Both RNA extraction and c DNA synthesis were performed using PrimeScript Hi‐Fide RT ‐ RCR Kit. Then, the prepared <t>cDNA</t> samples ( n = 5 c DNA samples/group) were methylated by bisulfite treatment using <t>Bisul‐Methylation</t> Universal Kit. Then, PCR amplification of Htr2c was performed, with the used primers designed by PyroMark Assay Design 2.0 and synthesized by Hua Da Gene Company, as shown in Table 1 . Subsequently, serial pyrosequencing measures were performed with the substrate mixture, enzyme mixture, and four types of d NTP added in the reaction system. Finally, pyrosequencing detector and Pyro Q‐CpG software were used to measure the frequency for A, B, D, and C/E editing sites
    Bisul Methylation Universal Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisul methylation universal kit/product/Qiagen
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bisul methylation universal kit - by Bioz Stars, 2020-04
    94/100 stars
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    99
    Qiagen pyromark pcr kit
    Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the <t>PyroMark</t> <t>PCR</t> Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed
    Pyromark Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyromark pcr kit/product/Qiagen
    Average 99 stars, based on 480 article reviews
    Price from $9.99 to $1999.99
    pyromark pcr kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Calibration curves generated from the predictions of the AQ module of the PyroMark Q96 2.5.8 software

    Journal: Algorithms for Molecular Biology : AMB

    Article Title: Using a constraint-based regression method for relative quantification of somatic mutations in pyrosequencing signals: a case for NRAS analysis

    doi: 10.1186/s13015-016-0086-4

    Figure Lengend Snippet: Calibration curves generated from the predictions of the AQ module of the PyroMark Q96 2.5.8 software

    Article Snippet: Amplification was performed in a 2720 Thermal Cycler (Applied Biosystems) using the following conditions: 95 °C for 5 min, followed by 40 cycles with denaturation at 95 °C for 40 s, annealing at 59 °C for 40 s, and extension at 72 °C for 80 s, with a final elongation step at 72 °C for 7 min. Pyrosequencing was then carried out with a PyroMark Q 96 ID Sequencer from Qiagen (Hilden, Germany) on PCR products, using the pyrosequencing primer, enzymes and substrate (PyroMark Gold®Q96 Reagents kit, Qiagen) according to the manufacturer’s protocol.

    Techniques: Generated, Software

    Outline of the procedure for measuring Htr2c RNA editing. Total RNA was extracted from the homogenized amygdala of the mice brain using the TRI zol method. Both RNA extraction and c DNA synthesis were performed using PrimeScript Hi‐Fide RT ‐ RCR Kit. Then, the prepared cDNA samples ( n = 5 c DNA samples/group) were methylated by bisulfite treatment using Bisul‐Methylation Universal Kit. Then, PCR amplification of Htr2c was performed, with the used primers designed by PyroMark Assay Design 2.0 and synthesized by Hua Da Gene Company, as shown in Table 1 . Subsequently, serial pyrosequencing measures were performed with the substrate mixture, enzyme mixture, and four types of d NTP added in the reaction system. Finally, pyrosequencing detector and Pyro Q‐CpG software were used to measure the frequency for A, B, D, and C/E editing sites

    Journal: Brain and Behavior

    Article Title: 5‐ HT2CR antagonist/5‐ HT2CR inverse agonist recovered the increased isolation‐induced aggressive behavior of BALB/c mice mediated by ADAR1 (p110) expression and Htr2c RNA editing, et al. 5‐HT2CR antagonist/5‐HT2CR inverse agonist recovered the increased isolation‐induced aggressive behavior of BALB/c mice mediated by ADAR1 (p110) expression and Htr2c RNA editing

    doi: 10.1002/brb3.929

    Figure Lengend Snippet: Outline of the procedure for measuring Htr2c RNA editing. Total RNA was extracted from the homogenized amygdala of the mice brain using the TRI zol method. Both RNA extraction and c DNA synthesis were performed using PrimeScript Hi‐Fide RT ‐ RCR Kit. Then, the prepared cDNA samples ( n = 5 c DNA samples/group) were methylated by bisulfite treatment using Bisul‐Methylation Universal Kit. Then, PCR amplification of Htr2c was performed, with the used primers designed by PyroMark Assay Design 2.0 and synthesized by Hua Da Gene Company, as shown in Table 1 . Subsequently, serial pyrosequencing measures were performed with the substrate mixture, enzyme mixture, and four types of d NTP added in the reaction system. Finally, pyrosequencing detector and Pyro Q‐CpG software were used to measure the frequency for A, B, D, and C/E editing sites

    Article Snippet: Then, the prepared cDNA samples (n = 5 cDNA samples/group) were methylated by bisulfite treatment using the Bisul‐Methylation Universal Kit (Qiagen, Germany).

    Techniques: Mouse Assay, RNA Extraction, DNA Synthesis, Methylation, Polymerase Chain Reaction, Amplification, Pyromark Assay, Synthesized, Software

    Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the PyroMark PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed

    Journal: BMC Neuroscience

    Article Title: Epigenetic evidence for involvement of the oxytocin receptor gene in obsessive–compulsive disorder

    doi: 10.1186/s12868-016-0313-4

    Figure Lengend Snippet: Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the PyroMark PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed

    Article Snippet: PM00016828, QIAGEN), using the PyroMark PCR Kit (QIAGEN).

    Techniques: Sequencing, Amplification, Polymerase Chain Reaction