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Marker Gene Technologies pyrf marker gene
Pyrf Marker Gene, supplied by Marker Gene Technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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pyrf marker gene - by Bioz Stars, 2020-08
91/100 stars

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pop-in/pop-out strategy
single crossover integration

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Plasmid Preparation:

Article Title: The Cdc45/RecJ-like protein forms a complex with GINS and MCM, and is important for DNA replication in Thermococcus kodakarensis
Article Snippet: .. We employed the pop-in/pop-out strategy that consists of a single crossover integration of a disruption plasmid into the chromosome followed by a second pop-out recombination that removes the pyrF marker gene ( ). ..

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    Marker Gene Technologies pyrf dpl200 marker gene
    Characterization of spontaneous <t>pyrF</t> - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the <t>pyrF-dpl200</t> control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.
    Pyrf Dpl200 Marker Gene, supplied by Marker Gene Technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyrf dpl200 marker gene/product/Marker Gene Technologies
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pyrf dpl200 marker gene - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Marker Gene Technologies pyrf marker gene
    Characterization of spontaneous <t>pyrF</t> - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the <t>pyrF-dpl200</t> control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.
    Pyrf Marker Gene, supplied by Marker Gene Technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyrf marker gene/product/Marker Gene Technologies
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pyrf marker gene - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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    Characterization of spontaneous pyrF - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the pyrF-dpl200 control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.

    Journal: Fungal genetics and biology : FG & B

    Article Title: CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar

    doi: 10.1016/j.fgb.2018.12.002

    Figure Lengend Snippet: Characterization of spontaneous pyrF - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the pyrF-dpl200 control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.

    Article Snippet: A 200-bp fragment from the 5′ UTR of the R. delemar pyrF gene was tandemly incorporated into the downstream, generating the pyrF-dpl200 marker gene ( ).

    Techniques: Incubation, Mutagenesis, DNA Sequencing, Amplification, Polymerase Chain Reaction, Clone Assay, Marker