Structured Review

Boehringer Mannheim pwo polymerase
) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type <t>HBV</t> DNA with <t>Taq-Pwo</t> ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.
Pwo Polymerase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pwo polymerase/product/Boehringer Mannheim
Average 92 stars, based on 47 article reviews
Price from $9.99 to $1999.99
pwo polymerase - by Bioz Stars, 2020-12
92/100 stars

Images

1) Product Images from "Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations"

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations

Journal: Journal of Clinical Microbiology

doi:

) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.
Figure Legend Snippet: ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.

Techniques Used: Plasmid Preparation, Clone Assay, Amplification, Transfection, Marker

2) Product Images from "Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations"

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations

Journal: Journal of Clinical Microbiology

doi:

) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.
Figure Legend Snippet: ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.

Techniques Used: Plasmid Preparation, Clone Assay, Amplification, Transfection, Marker

Related Articles

Clone Assay:

Article Title: Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli
Article Snippet: .. Operon fusions with lacZ were constructed by amplifying the regulatory region of b3243 with Pwo polymerase, using primers K2180 and K2179, and cloning into plasmid pRS551, which contains a promoterless lac operon , thereby generating plasmid pVS160. .. This fusion was transformed into host strains 86-24 (wild-type EHEC), VS94 ( luxS mutant of 86-24), and VS95 (VS94 complemented with luxS on a plasmid) (Tables and ).

Article Title: Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli
Article Snippet: .. Plasmid pVS130 was constructed by amplifying the b3243 gene from E. coli K-12 strain MG1655 with Pwo polymerase (Boehringer Mannheim), using primers K1977 and K1978, and cloning into ZERO BLUNT TOPO PCR vector (Invitrogen). .. Plasmid pVS150 was constructed by cloning b3243 digested with Xho I and Hin dIII from pVS130 into vector pACYC177 ( Xho I/ Hin dIII).

Amplification:

Article Title: Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2
Article Snippet: .. We amplified DIG3-3 DNA by PCR with Pwo polymerase (Boehringer Mannheim) by using the following primers: DT-3, 5′-ATAG CCATGG TCGATTATGCCTCTCCC GAATTC -3′ (forward) and DT-4, 5′-ACTG CCATGG CATACCAAAGCTT CTCGAG (reverse). .. Amplified DIG3 with the in-frame start codon ATG was digested with Nco I and cloned into the Nco I site of pYT-6 in the correct orientation to form pYT-7.

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. To determine which PCR system can be used for amplification of HBV genomes in sera from patients with low levels of viremia, defined copy numbers of plasmid-integrated HBV genomes were amplified with Pwo polymerase, which possesses proofreading activity , Taq polymerase, which lacks proofreading activity , and a Taq-Pwo polymerase mixture. .. The Pwo polymerase did not amplify less than 105 template molecules to a level detectable in an ethidium bromide-stained gel (Fig. A).

Article Title: Accelerated regulatory gene evolution in an adaptive radiation
Article Snippet: .. The primers were used in PCR amplification reactions using the error-correcting rTth polymerase formulation (Perkin–Elmer) or Pwo polymerase (Boehringer Mannheim) in standard buffer with cycling conditions recommended by the manufacturer. .. The nucleotide error rate for these error-correcting polymerases is less than 1 bp in 7 kb of sequence (J. I. Suddith and M.D.P., unpublished observations).

Construct:

Article Title: Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli
Article Snippet: .. Operon fusions with lacZ were constructed by amplifying the regulatory region of b3243 with Pwo polymerase, using primers K2180 and K2179, and cloning into plasmid pRS551, which contains a promoterless lac operon , thereby generating plasmid pVS160. .. This fusion was transformed into host strains 86-24 (wild-type EHEC), VS94 ( luxS mutant of 86-24), and VS95 (VS94 complemented with luxS on a plasmid) (Tables and ).

Article Title: Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli
Article Snippet: .. Plasmid pVS130 was constructed by amplifying the b3243 gene from E. coli K-12 strain MG1655 with Pwo polymerase (Boehringer Mannheim), using primers K1977 and K1978, and cloning into ZERO BLUNT TOPO PCR vector (Invitrogen). .. Plasmid pVS150 was constructed by cloning b3243 digested with Xho I and Hin dIII from pVS130 into vector pACYC177 ( Xho I/ Hin dIII).

Generated:

Article Title: Sequence-Specific Transcriptional Repression by KS1, a Multiple-Zinc-Finger-Kr?ppel-Associated Box Protein
Article Snippet: .. The double-stranded oligonucleotides used in the first round of DNA binding were generated by one cycle of PCR (3 min at 94°C, 2 min at 55°C, and 10 min at 72°C) using Pwo polymerase (Boehringer Mannheim, Indianapolis, Ind.) with a 10 M excess of reverse primer to random oligonucleotide. .. The PCR product was purified on a 3% low-melting-point agarose gel and end labeled with [γ-32 P]ATP by using T4 polynucleotide kinase according to the manufacturer's suggestions (Promega).

other:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: The combination of Pwo polymerase with Taq polymerase improved the fidelity of polymerization twofold, which is close to the threefold increase previously determined with a lacI -based assay ( ).

Activity Assay:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. To determine which PCR system can be used for amplification of HBV genomes in sera from patients with low levels of viremia, defined copy numbers of plasmid-integrated HBV genomes were amplified with Pwo polymerase, which possesses proofreading activity , Taq polymerase, which lacks proofreading activity , and a Taq-Pwo polymerase mixture. .. The Pwo polymerase did not amplify less than 105 template molecules to a level detectable in an ethidium bromide-stained gel (Fig. A).

Plasmid Preparation:

Article Title: Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli
Article Snippet: .. Operon fusions with lacZ were constructed by amplifying the regulatory region of b3243 with Pwo polymerase, using primers K2180 and K2179, and cloning into plasmid pRS551, which contains a promoterless lac operon , thereby generating plasmid pVS160. .. This fusion was transformed into host strains 86-24 (wild-type EHEC), VS94 ( luxS mutant of 86-24), and VS95 (VS94 complemented with luxS on a plasmid) (Tables and ).

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. To determine which PCR system can be used for amplification of HBV genomes in sera from patients with low levels of viremia, defined copy numbers of plasmid-integrated HBV genomes were amplified with Pwo polymerase, which possesses proofreading activity , Taq polymerase, which lacks proofreading activity , and a Taq-Pwo polymerase mixture. .. The Pwo polymerase did not amplify less than 105 template molecules to a level detectable in an ethidium bromide-stained gel (Fig. A).

Article Title: Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli
Article Snippet: .. Plasmid pVS130 was constructed by amplifying the b3243 gene from E. coli K-12 strain MG1655 with Pwo polymerase (Boehringer Mannheim), using primers K1977 and K1978, and cloning into ZERO BLUNT TOPO PCR vector (Invitrogen). .. Plasmid pVS150 was constructed by cloning b3243 digested with Xho I and Hin dIII from pVS130 into vector pACYC177 ( Xho I/ Hin dIII).

Polymerase Chain Reaction:

Article Title: Sequence-Specific Transcriptional Repression by KS1, a Multiple-Zinc-Finger-Kr?ppel-Associated Box Protein
Article Snippet: .. The double-stranded oligonucleotides used in the first round of DNA binding were generated by one cycle of PCR (3 min at 94°C, 2 min at 55°C, and 10 min at 72°C) using Pwo polymerase (Boehringer Mannheim, Indianapolis, Ind.) with a 10 M excess of reverse primer to random oligonucleotide. .. The PCR product was purified on a 3% low-melting-point agarose gel and end labeled with [γ-32 P]ATP by using T4 polynucleotide kinase according to the manufacturer's suggestions (Promega).

Article Title: Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2
Article Snippet: .. We amplified DIG3-3 DNA by PCR with Pwo polymerase (Boehringer Mannheim) by using the following primers: DT-3, 5′-ATAG CCATGG TCGATTATGCCTCTCCC GAATTC -3′ (forward) and DT-4, 5′-ACTG CCATGG CATACCAAAGCTT CTCGAG (reverse). .. Amplified DIG3 with the in-frame start codon ATG was digested with Nco I and cloned into the Nco I site of pYT-6 in the correct orientation to form pYT-7.

Article Title: Accelerated regulatory gene evolution in an adaptive radiation
Article Snippet: .. The primers were used in PCR amplification reactions using the error-correcting rTth polymerase formulation (Perkin–Elmer) or Pwo polymerase (Boehringer Mannheim) in standard buffer with cycling conditions recommended by the manufacturer. .. The nucleotide error rate for these error-correcting polymerases is less than 1 bp in 7 kb of sequence (J. I. Suddith and M.D.P., unpublished observations).

Article Title: Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli
Article Snippet: .. Plasmid pVS130 was constructed by amplifying the b3243 gene from E. coli K-12 strain MG1655 with Pwo polymerase (Boehringer Mannheim), using primers K1977 and K1978, and cloning into ZERO BLUNT TOPO PCR vector (Invitrogen). .. Plasmid pVS150 was constructed by cloning b3243 digested with Xho I and Hin dIII from pVS130 into vector pACYC177 ( Xho I/ Hin dIII).

Binding Assay:

Article Title: Sequence-Specific Transcriptional Repression by KS1, a Multiple-Zinc-Finger-Kr?ppel-Associated Box Protein
Article Snippet: .. The double-stranded oligonucleotides used in the first round of DNA binding were generated by one cycle of PCR (3 min at 94°C, 2 min at 55°C, and 10 min at 72°C) using Pwo polymerase (Boehringer Mannheim, Indianapolis, Ind.) with a 10 M excess of reverse primer to random oligonucleotide. .. The PCR product was purified on a 3% low-melting-point agarose gel and end labeled with [γ-32 P]ATP by using T4 polynucleotide kinase according to the manufacturer's suggestions (Promega).

Molecular Weight:

Article Title: SmcR-Dependent Regulation of Adaptive Phenotypes in Vibrio vulnificus
Article Snippet: .. Restriction enzymes, molecular weight markers, shrimp alkaline phosphatase, ligase, Pwo polymerase, and T4 DNA ligase were purchased from Boehringer Mannheim (Indianapolis, Ind.). ..

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  • 92
    Boehringer Mannheim pwo polymerase
    ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type <t>HBV</t> DNA with <t>Taq-Pwo</t> ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.
    Pwo Polymerase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pwo polymerase/product/Boehringer Mannheim
    Average 92 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    pwo polymerase - by Bioz Stars, 2020-12
    92/100 stars
      Buy from Supplier

    85
    Boehringer Mannheim expand high fidelity taq pwo dna polymerase mixture
    ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type <t>HBV</t> DNA with <t>Taq-Pwo</t> ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.
    Expand High Fidelity Taq Pwo Dna Polymerase Mixture, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity taq pwo dna polymerase mixture/product/Boehringer Mannheim
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity taq pwo dna polymerase mixture - by Bioz Stars, 2020-12
    85/100 stars
      Buy from Supplier

    Image Search Results


    ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.

    Journal: Journal of Clinical Microbiology

    Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations

    doi:

    Figure Lengend Snippet: ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.

    Article Snippet: To determine which PCR system can be used for amplification of HBV genomes in sera from patients with low levels of viremia, defined copy numbers of plasmid-integrated HBV genomes were amplified with Pwo polymerase, which possesses proofreading activity , Taq polymerase, which lacks proofreading activity , and a Taq-Pwo polymerase mixture.

    Techniques: Plasmid Preparation, Clone Assay, Amplification, Transfection, Marker