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Graded sparse labeling of distinct neuronal types in different brain regions. (A) Images showing graded sparse labeling of granule cells in the dentate gyrus (DG). (B, C) Images showing sparse labeling of parvalbumin (PV)‐positive neurons in the visual cortex (B) and cholinergic neurons in the basal forebrain (C) with the high sparsity mode of the Tri‐M system. Labeling specificity was confirmed by immunostaining <t>with</t> <t>anti‐PV</t> and anti‐ChAT, respectively. (D) A line graph showing labeling sparsity for different neuronal types across the three functional modes of the Tri‐M system, assessed 15 days after viral injection. Data are presented as mean ± SEM (n = 5 mice per group).
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Graded sparse labeling of distinct neuronal types in different brain regions. (A) Images showing graded sparse labeling of granule cells in the dentate gyrus (DG). (B, C) Images showing sparse labeling of parvalbumin (PV)‐positive neurons in the visual cortex (B) and cholinergic neurons in the basal forebrain (C) with the high sparsity mode of the Tri‐M system. Labeling specificity was confirmed by immunostaining <t>with</t> <t>anti‐PV</t> and anti‐ChAT, respectively. (D) A line graph showing labeling sparsity for different neuronal types across the three functional modes of the Tri‐M system, assessed 15 days after viral injection. Data are presented as mean ± SEM (n = 5 mice per group).
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Graded sparse labeling of distinct neuronal types in different brain regions. (A) Images showing graded sparse labeling of granule cells in the dentate gyrus (DG). (B, C) Images showing sparse labeling of parvalbumin (PV)‐positive neurons in the visual cortex (B) and cholinergic neurons in the basal forebrain (C) with the high sparsity mode of the Tri‐M system. Labeling specificity was confirmed by immunostaining <t>with</t> <t>anti‐PV</t> and anti‐ChAT, respectively. (D) A line graph showing labeling sparsity for different neuronal types across the three functional modes of the Tri‐M system, assessed 15 days after viral injection. Data are presented as mean ± SEM (n = 5 mice per group).
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Graded sparse labeling of distinct neuronal types in different brain regions. (A) Images showing graded sparse labeling of granule cells in the dentate gyrus (DG). (B, C) Images showing sparse labeling of parvalbumin (PV)‐positive neurons in the visual cortex (B) and cholinergic neurons in the basal forebrain (C) with the high sparsity mode of the Tri‐M system. Labeling specificity was confirmed by immunostaining <t>with</t> <t>anti‐PV</t> and anti‐ChAT, respectively. (D) A line graph showing labeling sparsity for different neuronal types across the three functional modes of the Tri‐M system, assessed 15 days after viral injection. Data are presented as mean ± SEM (n = 5 mice per group).
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Graded sparse labeling of distinct neuronal types in different brain regions. (A) Images showing graded sparse labeling of granule cells in the dentate gyrus (DG). (B, C) Images showing sparse labeling of parvalbumin (PV)‐positive neurons in the visual cortex (B) and cholinergic neurons in the basal forebrain (C) with the high sparsity mode of the Tri‐M system. Labeling specificity was confirmed by immunostaining with anti‐PV and anti‐ChAT, respectively. (D) A line graph showing labeling sparsity for different neuronal types across the three functional modes of the Tri‐M system, assessed 15 days after viral injection. Data are presented as mean ± SEM (n = 5 mice per group).

Journal: Advanced Science

Article Title: A Novel Sparse Cellular Labeling System with Tunable Gradients and Long‐Term Stability

doi: 10.1002/advs.77438

Figure Lengend Snippet: Graded sparse labeling of distinct neuronal types in different brain regions. (A) Images showing graded sparse labeling of granule cells in the dentate gyrus (DG). (B, C) Images showing sparse labeling of parvalbumin (PV)‐positive neurons in the visual cortex (B) and cholinergic neurons in the basal forebrain (C) with the high sparsity mode of the Tri‐M system. Labeling specificity was confirmed by immunostaining with anti‐PV and anti‐ChAT, respectively. (D) A line graph showing labeling sparsity for different neuronal types across the three functional modes of the Tri‐M system, assessed 15 days after viral injection. Data are presented as mean ± SEM (n = 5 mice per group).

Article Snippet: The following primary antibodies were used: chicken anti‐PV (1:500, SYSY, 195006), goat anti‐ChAT (1:500, Millipore, AB144P), and chicken anti‐GFAP (1:500, Invitrogen, PA1‐10004).

Techniques: Labeling, Immunostaining, Functional Assay, Injection