purified anti sox2  (BioLegend)

 
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    Name:
    Purified anti SOX2
    Description:
    Purified anti SOX2 Poly6519 Isotype Rabbit Polyclonal IgG Reactivity Human Apps WB IF Size 50 μl
    Catalog Number:
    651901
    Price:
    125
    Applications:
    WB, IF
    Conjugate:
    PURE
    Immunogen:
    Full length recombinant SOX2 protein
    Size:
    50 μl
    Category:
    Cell Biology
    Source:
    Rabbit
    Quantity:
    1
    Buy from Supplier


    Structured Review

    BioLegend purified anti sox2
    Purified anti SOX2
    Purified anti SOX2 Poly6519 Isotype Rabbit Polyclonal IgG Reactivity Human Apps WB IF Size 50 μl
    https://www.bioz.com/result/purified anti sox2/product/BioLegend
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    purified anti sox2 - by Bioz Stars, 2020-07
    86/100 stars

    Images

    Related Articles

    Electron Microscopy:

    Article Title: Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes
    Article Snippet: .. Cells were fixed with 4% PFA (Electron Microscopy Services) for 15 min at RT, permeabilized with 0.1% Triton-X (Sigma-Aldrich) for 10 min at RT, blocked in 10% goat serum (Sigma-Aldrich) and 0.1% Triton-X for 15 min at RT and stained with 1:200 mouse IgM TRA-1–60 (09–0010), 1:200 mouse IgMk TRA-1–80 (09–0012), 1:200 mouse IgG3 SSEA4 (09-0006, all Stemgent), 1:200 rabbit IgG POU5F1 (sc-9081), 1:200 rabbit IgG NANOG (sc-33759, both Santa Cruz Biotechnology), 1:200 rabbit IgG SOX2 (651901, Biolegend), 1:200 mouse monoclonal IgG1 TNNT2 (13-11, Thermo Scientific), 1:200 rabbit monoclonal IgG α-actinin (H-300, Santa Cruz), 1:400 mouse monoclonal IgG2b MLC2A (MYL7, Synaptic Systems), 1:200 rabbit polyclonal IgG MLC2V (MYL2, ProteinTech), 1:50 mouse monoclonal IgG1 P4HB (Abcam), 1:400 mouse monoclonal IgG2a SMA (Sigma-Aldrich), 1:400 rabbit polyclonal IgG vWF (Abcam), or 1:500 rabbit polyclonal IgG Ki67 (Thermo Scientific) overnight at 4 °C in 1% BSA in D-PBS. .. Cells were washed 4 times, for 10 min, with 1% BSA in D-PBS-T and then incubated for 1 hour at RT in the dark with secondary antibodies 1:1000 Alexa Fluor 488 goat anti-mouse IgM (µ chain), 1:1000 Alexa Fluor 488 goat anti-mouse IgG (H+L), or 1:1000 Alexa Fluor 594 goat anti-rabbit IgG (H+L) (all Life Technologies) in 1% BSA in D-PBS.

    Staining:

    Article Title: Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes
    Article Snippet: .. Cells were fixed with 4% PFA (Electron Microscopy Services) for 15 min at RT, permeabilized with 0.1% Triton-X (Sigma-Aldrich) for 10 min at RT, blocked in 10% goat serum (Sigma-Aldrich) and 0.1% Triton-X for 15 min at RT and stained with 1:200 mouse IgM TRA-1–60 (09–0010), 1:200 mouse IgMk TRA-1–80 (09–0012), 1:200 mouse IgG3 SSEA4 (09-0006, all Stemgent), 1:200 rabbit IgG POU5F1 (sc-9081), 1:200 rabbit IgG NANOG (sc-33759, both Santa Cruz Biotechnology), 1:200 rabbit IgG SOX2 (651901, Biolegend), 1:200 mouse monoclonal IgG1 TNNT2 (13-11, Thermo Scientific), 1:200 rabbit monoclonal IgG α-actinin (H-300, Santa Cruz), 1:400 mouse monoclonal IgG2b MLC2A (MYL7, Synaptic Systems), 1:200 rabbit polyclonal IgG MLC2V (MYL2, ProteinTech), 1:50 mouse monoclonal IgG1 P4HB (Abcam), 1:400 mouse monoclonal IgG2a SMA (Sigma-Aldrich), 1:400 rabbit polyclonal IgG vWF (Abcam), or 1:500 rabbit polyclonal IgG Ki67 (Thermo Scientific) overnight at 4 °C in 1% BSA in D-PBS. .. Cells were washed 4 times, for 10 min, with 1% BSA in D-PBS-T and then incubated for 1 hour at RT in the dark with secondary antibodies 1:1000 Alexa Fluor 488 goat anti-mouse IgM (µ chain), 1:1000 Alexa Fluor 488 goat anti-mouse IgG (H+L), or 1:1000 Alexa Fluor 594 goat anti-rabbit IgG (H+L) (all Life Technologies) in 1% BSA in D-PBS.

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  • 91
    BioLegend sox2
    Revertant iPSCs from RDEB skin (a) Persistent mRNA expression of OCT4, <t>SOX2</t> , NANOG , LIN28 , and DNMT3b , and transient mRNA expression of c-MYC and KLF4 , both consistent with fully reprogrammed mutant and revertant iPSCs. (b) Protein expression in mutant iPSCs of embryonic stem cell surface markers TRA-1-60, TRA-1-81, SSEA3, SSEA4, alkaline phosphatase (AF), and transcription factors Nanog and OCT3/4. (c) Same embryonic stem cell protein expression panel in revertant iPSCs. Nuclei are stained with DAPI (blue). Lower panels show corresponding isotype controls. Scale bar = 50 μm.
    Sox2, supplied by BioLegend, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sox2/product/BioLegend
    Average 91 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    sox2 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    86
    BioLegend purified anti sox2
    Revertant iPSCs from RDEB skin (a) Persistent mRNA expression of OCT4, <t>SOX2</t> , NANOG , LIN28 , and DNMT3b , and transient mRNA expression of c-MYC and KLF4 , both consistent with fully reprogrammed mutant and revertant iPSCs. (b) Protein expression in mutant iPSCs of embryonic stem cell surface markers TRA-1-60, TRA-1-81, SSEA3, SSEA4, alkaline phosphatase (AF), and transcription factors Nanog and OCT3/4. (c) Same embryonic stem cell protein expression panel in revertant iPSCs. Nuclei are stained with DAPI (blue). Lower panels show corresponding isotype controls. Scale bar = 50 μm.
    Purified Anti Sox2, supplied by BioLegend, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/purified anti sox2/product/BioLegend
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    purified anti sox2 - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Revertant iPSCs from RDEB skin (a) Persistent mRNA expression of OCT4, SOX2 , NANOG , LIN28 , and DNMT3b , and transient mRNA expression of c-MYC and KLF4 , both consistent with fully reprogrammed mutant and revertant iPSCs. (b) Protein expression in mutant iPSCs of embryonic stem cell surface markers TRA-1-60, TRA-1-81, SSEA3, SSEA4, alkaline phosphatase (AF), and transcription factors Nanog and OCT3/4. (c) Same embryonic stem cell protein expression panel in revertant iPSCs. Nuclei are stained with DAPI (blue). Lower panels show corresponding isotype controls. Scale bar = 50 μm.

    Journal: The Journal of investigative dermatology

    Article Title: Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa

    doi: 10.1038/jid.2013.523

    Figure Lengend Snippet: Revertant iPSCs from RDEB skin (a) Persistent mRNA expression of OCT4, SOX2 , NANOG , LIN28 , and DNMT3b , and transient mRNA expression of c-MYC and KLF4 , both consistent with fully reprogrammed mutant and revertant iPSCs. (b) Protein expression in mutant iPSCs of embryonic stem cell surface markers TRA-1-60, TRA-1-81, SSEA3, SSEA4, alkaline phosphatase (AF), and transcription factors Nanog and OCT3/4. (c) Same embryonic stem cell protein expression panel in revertant iPSCs. Nuclei are stained with DAPI (blue). Lower panels show corresponding isotype controls. Scale bar = 50 μm.

    Article Snippet: The following antibodies were used: TRA1-60 (clone MAB4360, 1:400), TRA1-81 (clone MAB4381, 1:400), SSEA4 (clone MAB4304, 1:100) and SSEA3 (clone MAB-4303, 1:100) from Millipore; NANOG (clone EB06860, 1:100) from Everest Biotech, Upper Heyford, Oxfordshire, UK; OCT3/4 (clone AB27985, 1:200) from ABCAM, Cambridge, MA; and SOX2 (clone 630802, 1:500) from Biolegend, San Diego, CA.

    Techniques: Expressing, Mutagenesis, Staining