pulsed field certified agarose gels  (Bio-Rad)

 
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    Name:
    Pulsed Field Certified Agarose
    Description:
    100 g agarose for separation of large DNA in pulsed field gel applications certified free of inhibitors DNases RNases
    Catalog Number:
    1620137
    Price:
    None
    Category:
    CHEF Mapper XA System
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    Bio-Rad pulsed field certified agarose gels
    Pulsed Field Certified Agarose
    100 g agarose for separation of large DNA in pulsed field gel applications certified free of inhibitors DNases RNases
    https://www.bioz.com/result/pulsed field certified agarose gels/product/Bio-Rad
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pulsed field certified agarose gels - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Assessment of Strain Relatedness among Salmonella Serotypes Salinatis, Duisburg, and Sandiego by Biotyping, Ribotyping, IS200 Fingerprinting, and Pulsed-Field Gel Electrophoresis
    Article Snippet: .. Electrophoresis of digestion products was carried out in 1% (wt/vol) pulsed-field-certified agarose gels in a contour-clamped homogeneous electric field DRII system (Bio-Rad) with 0.5× TBE buffer (pH 8.3) at 14°C. .. Run conditions were 6 V/cm for 23 h with an initial switch time of 5 s and a final switch time of 60 s. Gels were stained in ethidium bromide (0.5 μg/ml in 0.5× TBE) and photographed by UV transillumination (312 nm) on Polaroid 667 film.

    Staining:

    Article Title: Forward Genetics Approach Reveals Host Genotype-Dependent Importance of Accessory Chromosomes in the Fungal Wheat Pathogen Zymoseptoria tritici
    Article Snippet: .. Small chromosomes were separated in 1.2% pulsed-field agarose (Bio-Rad, Munich, Germany)–0.5× TBE buffer and were processed for 48 h at 14°C using an angle of 120°, 5 V/cm, and a switching time of 50 to 150 s. Midsize chromosomes were separated in 1% pulsed-field agarose–1× TBE buffer and were processed for 72 h at 14°C using an angle of 106°, 3 V/cm, and a switching time of 250 to 1,000 s. Large chromosomes were separated in 0.8% pulsed-field agarose–1× TRIS-acetate-EDTA (TAE) buffer and were processed for 92 h at 13°C using an angle of 106°, 2 V/cm, and a switching time of 1,000 to 2,000 s. Gels were stained for 30 min in a 0.5 µg/ml ethidium bromide solution followed by 10 min of destaining in H2 O. ..

    Agarose Gel Electrophoresis:

    Article Title: Repeat-Associated Fission Yeast-Like Regional Centromeres in the Ascomycetous Budding Yeast Candida tropicalis
    Article Snippet: .. A 0.6% pulsed field certified agarose gel was prepared using 0.5X TBE buffer (0.1 M Tris, 0.09 M boric acid, 0.01 M EDTA, pH 8) and the PFGE was performed on a CHEF-DR II (Bio-Rad) for 72 h (24 h at 4.5 V/cm/106° with an initial and final switch times 200 s; 48 h at 3 V/cm/106° with an initial and final switch time 700 s). .. The gel was stained with ethidium bromide (EtBr) and analyzed by using the Quantity One software (Bio-Rad).

    Article Title: Analysis of the Genome of a Korean Isolate of the Pieris rapae Granulovirus Enabled by Its Separation from Total Host Genomic DNA by Pulse-Field Electrophoresis
    Article Snippet: .. Subsequently, they were inserted into the preparative slot of 1% pulse- field certified agarose gel, and PFGE was conducted using 0.5× TBE buffer and the CHEF DR-II apparatus (Bio-Rad, Hercules, CA) with a pulse time of 5 s for 10 hr at 12o C and a voltage of 4V/cm. .. After the electrophoresis, the plugs were removed from the slot, stored in 50 ml of 0.5 M EDTA buffer, and dialyzed overnight at 4o C.

    Article Title: Necrotoxigenic Escherichia coli type-2 invade and cause diarrhoea during experimental infection in colostrum-restricted newborn calves.
    Article Snippet: .. After 5 h, the supernatant was removed and replaced by 250 ml of EDTA 0.5 M. The plugs were then loaded into the wells of a 1% pulsed-field certified agarose gel (BioRad) in 0.5X TBE buffer (BioRad). .. The gel was processed by using the CHEF MAPPER apparatus (BioRad).

    Article Title: Dynamics of Bacillus thuringiensis var. israelensis and Lysinibacillus sphaericus Spores in Urban Catch Basins after Simultaneous Application against Mosquito Larvae
    Article Snippet: .. Restriction fragments were separated in a 1% Pulsed-Field Certified agarose gel (Bio-Rad) in 0.5 Tris-borate-EDTA (TBE) buffer containing 50 µM Thiourea (Sigma), using CHEF-DR III system (Bio-Rad Laboratories). .. The following conditions were used for the separation of the digested fragments: pulse time: 3 to 35.4 sec., linear ramping, 6 V cm−1 , 22 h at 14°C, 120° included angle.

    Article Title: Role of integrons, plasmids and SXT elements in multidrug resistance of Vibrio cholerae and Providencia vermicola obtained from a clinical isolate of diarrhea
    Article Snippet: .. For PFGE, 1% Bio-Rad pulsed field certified agarose gel (Bio-Rad Laboratories, Richmond, CA, USA) was prepared in 0.5X TBE and run in CHEF MAPPER (Bio-Rad Laboratories) using autoalgorithm mode (molecular weight range: 100–350 K and a run time of 19 h). .. The gel was stained with 0.05 mg ml-1 ethidium bromide for 30 min and destained with sterile water for 1 h. RAPD analysis was carried out by 1281 and 1283 primers as described earlier ( ; ).

    Molecular Weight:

    Article Title: Role of integrons, plasmids and SXT elements in multidrug resistance of Vibrio cholerae and Providencia vermicola obtained from a clinical isolate of diarrhea
    Article Snippet: .. For PFGE, 1% Bio-Rad pulsed field certified agarose gel (Bio-Rad Laboratories, Richmond, CA, USA) was prepared in 0.5X TBE and run in CHEF MAPPER (Bio-Rad Laboratories) using autoalgorithm mode (molecular weight range: 100–350 K and a run time of 19 h). .. The gel was stained with 0.05 mg ml-1 ethidium bromide for 30 min and destained with sterile water for 1 h. RAPD analysis was carried out by 1281 and 1283 primers as described earlier ( ; ).

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  • 99
    Bio-Rad pulsed field certified agarose gels
    Pulsed Field Certified Agarose Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pulsed field certified agarose gels/product/Bio-Rad
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pulsed field certified agarose gels - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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