Structured Review

GenScript puc57
The lncRNA CHRF promotes silica-induced pulmonary fibrosis through targeting miR-489. (A) Western blot analysis of MyD88, IL-1β and TGF-β1 expression in RAW 264.7 cells and (B) total Smad3 and p-Smad3 expression in NIH/3T3 cells transfected with <t>pUC57</t> plasmids of CHRF with * P
Puc57, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 22 article reviews
Price from $9.99 to $1999.99
puc57 - by Bioz Stars, 2020-09
93/100 stars

Images

1) Product Images from "miR-489 inhibits silica-induced pulmonary fibrosis by targeting MyD88 and Smad3 and is negatively regulated by lncRNA CHRF"

Article Title: miR-489 inhibits silica-induced pulmonary fibrosis by targeting MyD88 and Smad3 and is negatively regulated by lncRNA CHRF

Journal: Scientific Reports

doi: 10.1038/srep30921

The lncRNA CHRF promotes silica-induced pulmonary fibrosis through targeting miR-489. (A) Western blot analysis of MyD88, IL-1β and TGF-β1 expression in RAW 264.7 cells and (B) total Smad3 and p-Smad3 expression in NIH/3T3 cells transfected with pUC57 plasmids of CHRF with * P
Figure Legend Snippet: The lncRNA CHRF promotes silica-induced pulmonary fibrosis through targeting miR-489. (A) Western blot analysis of MyD88, IL-1β and TGF-β1 expression in RAW 264.7 cells and (B) total Smad3 and p-Smad3 expression in NIH/3T3 cells transfected with pUC57 plasmids of CHRF with * P

Techniques Used: Western Blot, Expressing, Transfection

2) Product Images from "Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis"

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0097100

A fragment containing an inverted repeat (IR, black bar), the promoter (p), the signal sequence(s) and an FRT site (grey bar) was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR using the Eco RI and Xho I cleavage sites in a pUC57 backbone. Tn 4001 with one or both insertion sequences was amplified and inserted in between the FRT sites of the construct to generate pTn 4001 single (a) and pTn 4001 complete (b), respectively. The construct pMiniTn 4001 -gent (c) was developed by amplifying and inserting the gentamicin resistance gene ( aacA-aphD ) between the two FRT sites of the construct, then the transposase gene ( tnp ) was amplified and inserted outside of the transposable element (IR, black bar). To generate the plasmid pMiniTn 4001 -tet (d), a fragment containing the IR, the promoter (p), the signal (s) and an FRT site was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR in the pUC57 plasmid backbone. FRT sites have a unique Xba I cleav age site, so ligation of the fragments produced a construct with a single FRT site. The tnp gene was amplified and ligated into the plasmid outside the transposing element, then the tetM resistance gene with its own promoter and terminator was ligated within the construct.
Figure Legend Snippet: A fragment containing an inverted repeat (IR, black bar), the promoter (p), the signal sequence(s) and an FRT site (grey bar) was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR using the Eco RI and Xho I cleavage sites in a pUC57 backbone. Tn 4001 with one or both insertion sequences was amplified and inserted in between the FRT sites of the construct to generate pTn 4001 single (a) and pTn 4001 complete (b), respectively. The construct pMiniTn 4001 -gent (c) was developed by amplifying and inserting the gentamicin resistance gene ( aacA-aphD ) between the two FRT sites of the construct, then the transposase gene ( tnp ) was amplified and inserted outside of the transposable element (IR, black bar). To generate the plasmid pMiniTn 4001 -tet (d), a fragment containing the IR, the promoter (p), the signal (s) and an FRT site was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR in the pUC57 plasmid backbone. FRT sites have a unique Xba I cleav age site, so ligation of the fragments produced a construct with a single FRT site. The tnp gene was amplified and ligated into the plasmid outside the transposing element, then the tetM resistance gene with its own promoter and terminator was ligated within the construct.

Techniques Used: Sequencing, Amplification, Construct, Plasmid Preparation, Ligation, Produced

3) Product Images from "Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis"

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0097100

A fragment containing an inverted repeat (IR, black bar), the promoter (p), the signal sequence(s) and an FRT site (grey bar) was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR using the Eco RI and Xho I cleavage sites in a pUC57 backbone. Tn 4001 with one or both insertion sequences was amplified and inserted in between the FRT sites of the construct to generate pTn 4001 single (a) and pTn 4001 complete (b), respectively. The construct pMiniTn 4001 -gent (c) was developed by amplifying and inserting the gentamicin resistance gene ( aacA-aphD ) between the two FRT sites of the construct, then the transposase gene ( tnp ) was amplified and inserted outside of the transposable element (IR, black bar). To generate the plasmid pMiniTn 4001 -tet (d), a fragment containing the IR, the promoter (p), the signal (s) and an FRT site was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR in the pUC57 plasmid backbone. FRT sites have a unique Xba I cleav age site, so ligation of the fragments produced a construct with a single FRT site. The tnp gene was amplified and ligated into the plasmid outside the transposing element, then the tetM resistance gene with its own promoter and terminator was ligated within the construct.
Figure Legend Snippet: A fragment containing an inverted repeat (IR, black bar), the promoter (p), the signal sequence(s) and an FRT site (grey bar) was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR using the Eco RI and Xho I cleavage sites in a pUC57 backbone. Tn 4001 with one or both insertion sequences was amplified and inserted in between the FRT sites of the construct to generate pTn 4001 single (a) and pTn 4001 complete (b), respectively. The construct pMiniTn 4001 -gent (c) was developed by amplifying and inserting the gentamicin resistance gene ( aacA-aphD ) between the two FRT sites of the construct, then the transposase gene ( tnp ) was amplified and inserted outside of the transposable element (IR, black bar). To generate the plasmid pMiniTn 4001 -tet (d), a fragment containing the IR, the promoter (p), the signal (s) and an FRT site was ligated to a fragment containing an FRT site, the reporter gene ( phoA ) and an IR in the pUC57 plasmid backbone. FRT sites have a unique Xba I cleav age site, so ligation of the fragments produced a construct with a single FRT site. The tnp gene was amplified and ligated into the plasmid outside the transposing element, then the tetM resistance gene with its own promoter and terminator was ligated within the construct.

Techniques Used: Sequencing, Amplification, Construct, Plasmid Preparation, Ligation, Produced

4) Product Images from "Creation of a Nonspreading Rift Valley Fever Virus ▿"

Article Title: Creation of a Nonspreading Rift Valley Fever Virus ▿

Journal: Journal of Virology

doi: 10.1128/JVI.00841-11

Expression of the N protein from the antigenomic-sense S segment. BSR-T7/5 cells (A) or FP-T7-infected BHK-21 cells (B) were transfected with plasmid pUC57-S, containing the RVFV S genome segment in antigenomic-sense orientation. Expression of the RVFV N protein was detected using an N-protein-specific MAb and horseradish peroxidase-conjugated anti-mouse IgG antibodies.
Figure Legend Snippet: Expression of the N protein from the antigenomic-sense S segment. BSR-T7/5 cells (A) or FP-T7-infected BHK-21 cells (B) were transfected with plasmid pUC57-S, containing the RVFV S genome segment in antigenomic-sense orientation. Expression of the RVFV N protein was detected using an N-protein-specific MAb and horseradish peroxidase-conjugated anti-mouse IgG antibodies.

Techniques Used: Expressing, Infection, Transfection, Plasmid Preparation

Production of RRPs by the three-plasmid system. (A) BHK cells were infected with FP-T7 and subsequently remained untreated (mock) or were transfected with plasmid pUC57-S-eGFP (S-eGFP) only, in combination with plasmid pUC57-L encoding the RVFV L genome segment (S-eGFP/L), or with the aforementioned plasmids and pCAGGS-M (GP), encoding the structural glycoproteins (S-eGFP/L/GP). The percentage of eGFP-positive cells was determined by flow cytometry (values are means and standard deviations for three replicates). (B) Schematic representation of the three-plasmid system. BHK cells were first infected with FP-T7 (step 1) and subsequently transfected with transcription plasmids pUC57-L (L) and pUC57-S-eGFP (S) and expression plasmid pCAGGS-M (step 2). After 24 h, the culture medium containing the RRPs was collected (step 3). Transcription from the expression plasmid is controlled by a CAG promoter (CAGp), and transcription from the transcription plasmids is controlled by a T7 promoter (T7p). Untranslated regions are depicted as black boxes.
Figure Legend Snippet: Production of RRPs by the three-plasmid system. (A) BHK cells were infected with FP-T7 and subsequently remained untreated (mock) or were transfected with plasmid pUC57-S-eGFP (S-eGFP) only, in combination with plasmid pUC57-L encoding the RVFV L genome segment (S-eGFP/L), or with the aforementioned plasmids and pCAGGS-M (GP), encoding the structural glycoproteins (S-eGFP/L/GP). The percentage of eGFP-positive cells was determined by flow cytometry (values are means and standard deviations for three replicates). (B) Schematic representation of the three-plasmid system. BHK cells were first infected with FP-T7 (step 1) and subsequently transfected with transcription plasmids pUC57-L (L) and pUC57-S-eGFP (S) and expression plasmid pCAGGS-M (step 2). After 24 h, the culture medium containing the RRPs was collected (step 3). Transcription from the expression plasmid is controlled by a CAG promoter (CAGp), and transcription from the transcription plasmids is controlled by a T7 promoter (T7p). Untranslated regions are depicted as black boxes.

Techniques Used: Plasmid Preparation, Infection, Transfection, Flow Cytometry, Cytometry, Expressing

Related Articles

Clone Assay:

Article Title: A unifying mechanism for the biogenesis of membrane proteins co-operatively integrated by the Sec and Tat pathways
Article Snippet: .. A synthetic gene encoding the transmembrane region (residues 1–227) of the Rieske protein (QcrA) from Mycobacterium tuberculosis strain Rv2195 was codon optimised for E. coli K12 expression (OPTIMIZER, [ ]) and the synthetic gene was purchased ready cloned in pUC57 (GenScript). .. The MtbRieskeTMD coding region was subcloned by digestion Rca I-Xba I and ligated into pBAD24 ( ) using vector sites Nco I/Xba I.

Article Title: Characterization of the Salmonella enterica Serovar Typhimurium ydcI Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ †
Article Snippet: .. Plasmid pBAD18+ ydcI was constructed using a synthesized ydcI gene in which a six-histidine tag was fused to the C terminus and cloned into pUC57 ( ) and then subcloned into pBAD18 ( ) downstream of the arabinose-inducible promoter (Genscript, Inc., Piscataway, NJ). ..

Article Title: Efficient production of glycyrrhetinic acid in metabolically engineered Saccharomyces cerevisiae via an integrated strategy
Article Snippet: .. To improve gene expression in S. cerevisiae we optimized the codons CYP88D6 (OP-CYP88D6 ) and CYP72A154 (OP-CYP72A154 ) for genes cloning into pUC57, which were synthesized by GenScript (GenScript, Nanjing, China). .. We constructed pUC19L-Ptdh3-Tcyc1, pUC19L -Ppgk1-Tadh1, pUC19L-Ptef1-Tpgk1, and pUC19L-Ptef2-Tcyc1 utilizing seamless cloning and assembly by implementing the pEASY-UniSeamless Cloning and Assembly kit (TransGen Biotech, Beijing, China) (Additional file : Fig. S9a).

Article Title: Creation of a Nonspreading Rift Valley Fever Virus ▿
Article Snippet: .. The consensus sequences corresponding to each genome segment were synthesized and cloned in pUC57, a standard cloning vector of the GenScript Corporation (Piscataway, NJ). pUC57-L, pUC57-M, and pUC57-S encode the RVFV L, M, and S genome segments, respectively, in antigenomic orientation. .. The complete sequence of the L, M, and S genome sequences can be found in GenBank under the accession numbers , , and , respectively.

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis
Article Snippet: .. Development of novel reporter construct To create a novel transposon from which the antibiotic resistance marker could be excised following transposon insertion in, and disruption of, a specific gene, operator and gene region fragments were designed and then synthesised commercially and cloned in the Eco RV site of pUC57 (GenScript Corporation). .. The operator region contained an inverted repeat (IR) (39 bp, 5′-gataaagtccgtataattgtgtaaaagtaaaaaggccat-3′) together with the M. bovis tuf promoter (252 bp tuf promoter region located between bases 474270 and 474521 of NCBI Reference Sequence NC_014760.1), a vsp signal sequence (84 bp, gene ID 10014768, predicted protein sequence MKKSKFLLLGSVASLASIPFVAAKCGET ) and the FRT sequence (34 bp Flp recognition target, 5′-gaagttcctattctctagaaagtataggaacttc-3′ ).

Transfection:

Article Title: miR-489 inhibits silica-induced pulmonary fibrosis by targeting MyD88 and Smad3 and is negatively regulated by lncRNA CHRF
Article Snippet: .. Plasmids construction and cell transfection The CHRF lncRNA sequence was synthesized and subcloned into pUC57 to generate CHRF plasmids (GenScript, Nanjing, China). .. The overexpression of CHRF was achieved via transfection with CHRF plasmids into RAW 264.7 and NIH/3T3 cells.

Ligation:

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis
Article Snippet: .. As the FRT sequences contain a single Xba I cleavage site, ligation of the operator and gene segments after digestion with Sac I and Xba I produced a single FRT site ( ) in the construct, with pUC57 as the backbone. ..

Synthesized:

Article Title: Characterization of the Salmonella enterica Serovar Typhimurium ydcI Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ †
Article Snippet: .. Plasmid pBAD18+ ydcI was constructed using a synthesized ydcI gene in which a six-histidine tag was fused to the C terminus and cloned into pUC57 ( ) and then subcloned into pBAD18 ( ) downstream of the arabinose-inducible promoter (Genscript, Inc., Piscataway, NJ). ..

Article Title: miR-489 inhibits silica-induced pulmonary fibrosis by targeting MyD88 and Smad3 and is negatively regulated by lncRNA CHRF
Article Snippet: .. Plasmids construction and cell transfection The CHRF lncRNA sequence was synthesized and subcloned into pUC57 to generate CHRF plasmids (GenScript, Nanjing, China). .. The overexpression of CHRF was achieved via transfection with CHRF plasmids into RAW 264.7 and NIH/3T3 cells.

Article Title: Efficient production of glycyrrhetinic acid in metabolically engineered Saccharomyces cerevisiae via an integrated strategy
Article Snippet: .. To improve gene expression in S. cerevisiae we optimized the codons CYP88D6 (OP-CYP88D6 ) and CYP72A154 (OP-CYP72A154 ) for genes cloning into pUC57, which were synthesized by GenScript (GenScript, Nanjing, China). .. We constructed pUC19L-Ptdh3-Tcyc1, pUC19L -Ppgk1-Tadh1, pUC19L-Ptef1-Tpgk1, and pUC19L-Ptef2-Tcyc1 utilizing seamless cloning and assembly by implementing the pEASY-UniSeamless Cloning and Assembly kit (TransGen Biotech, Beijing, China) (Additional file : Fig. S9a).

Article Title: Creation of a Nonspreading Rift Valley Fever Virus ▿
Article Snippet: .. The consensus sequences corresponding to each genome segment were synthesized and cloned in pUC57, a standard cloning vector of the GenScript Corporation (Piscataway, NJ). pUC57-L, pUC57-M, and pUC57-S encode the RVFV L, M, and S genome segments, respectively, in antigenomic orientation. .. The complete sequence of the L, M, and S genome sequences can be found in GenBank under the accession numbers , , and , respectively.

Construct:

Article Title: Characterization of the Salmonella enterica Serovar Typhimurium ydcI Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ †
Article Snippet: .. Plasmid pBAD18+ ydcI was constructed using a synthesized ydcI gene in which a six-histidine tag was fused to the C terminus and cloned into pUC57 ( ) and then subcloned into pBAD18 ( ) downstream of the arabinose-inducible promoter (Genscript, Inc., Piscataway, NJ). ..

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis
Article Snippet: .. As the FRT sequences contain a single Xba I cleavage site, ligation of the operator and gene segments after digestion with Sac I and Xba I produced a single FRT site ( ) in the construct, with pUC57 as the backbone. ..

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis
Article Snippet: .. Development of novel reporter construct To create a novel transposon from which the antibiotic resistance marker could be excised following transposon insertion in, and disruption of, a specific gene, operator and gene region fragments were designed and then synthesised commercially and cloned in the Eco RV site of pUC57 (GenScript Corporation). .. The operator region contained an inverted repeat (IR) (39 bp, 5′-gataaagtccgtataattgtgtaaaagtaaaaaggccat-3′) together with the M. bovis tuf promoter (252 bp tuf promoter region located between bases 474270 and 474521 of NCBI Reference Sequence NC_014760.1), a vsp signal sequence (84 bp, gene ID 10014768, predicted protein sequence MKKSKFLLLGSVASLASIPFVAAKCGET ) and the FRT sequence (34 bp Flp recognition target, 5′-gaagttcctattctctagaaagtataggaacttc-3′ ).

Produced:

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis
Article Snippet: .. As the FRT sequences contain a single Xba I cleavage site, ligation of the operator and gene segments after digestion with Sac I and Xba I produced a single FRT site ( ) in the construct, with pUC57 as the backbone. ..

Sequencing:

Article Title: miR-489 inhibits silica-induced pulmonary fibrosis by targeting MyD88 and Smad3 and is negatively regulated by lncRNA CHRF
Article Snippet: .. Plasmids construction and cell transfection The CHRF lncRNA sequence was synthesized and subcloned into pUC57 to generate CHRF plasmids (GenScript, Nanjing, China). .. The overexpression of CHRF was achieved via transfection with CHRF plasmids into RAW 264.7 and NIH/3T3 cells.

Generated:

Article Title: Engineering HIV-1-Resistant T-Cells from Short-Hairpin RNA-Expressing Hematopoietic Stem/Progenitor Cells in Humanized BLT Mice
Article Snippet: .. To generate 7SK-promoter-driven sh516 expression cassette, pUC57 possessing the 7SK promoter followed by multiple restriction enzyme sites was generated by Genescript (Piscataway, NJ). .. Next, oligos GR10 and GR11 were synthesized, annealed, and cloned downstream of the 7SK promoter.

Expressing:

Article Title: A unifying mechanism for the biogenesis of membrane proteins co-operatively integrated by the Sec and Tat pathways
Article Snippet: .. A synthetic gene encoding the transmembrane region (residues 1–227) of the Rieske protein (QcrA) from Mycobacterium tuberculosis strain Rv2195 was codon optimised for E. coli K12 expression (OPTIMIZER, [ ]) and the synthetic gene was purchased ready cloned in pUC57 (GenScript). .. The MtbRieskeTMD coding region was subcloned by digestion Rca I-Xba I and ligated into pBAD24 ( ) using vector sites Nco I/Xba I.

Article Title: Engineering HIV-1-Resistant T-Cells from Short-Hairpin RNA-Expressing Hematopoietic Stem/Progenitor Cells in Humanized BLT Mice
Article Snippet: .. To generate 7SK-promoter-driven sh516 expression cassette, pUC57 possessing the 7SK promoter followed by multiple restriction enzyme sites was generated by Genescript (Piscataway, NJ). .. Next, oligos GR10 and GR11 were synthesized, annealed, and cloned downstream of the 7SK promoter.

Article Title: Efficient production of glycyrrhetinic acid in metabolically engineered Saccharomyces cerevisiae via an integrated strategy
Article Snippet: .. To improve gene expression in S. cerevisiae we optimized the codons CYP88D6 (OP-CYP88D6 ) and CYP72A154 (OP-CYP72A154 ) for genes cloning into pUC57, which were synthesized by GenScript (GenScript, Nanjing, China). .. We constructed pUC19L-Ptdh3-Tcyc1, pUC19L -Ppgk1-Tadh1, pUC19L-Ptef1-Tpgk1, and pUC19L-Ptef2-Tcyc1 utilizing seamless cloning and assembly by implementing the pEASY-UniSeamless Cloning and Assembly kit (TransGen Biotech, Beijing, China) (Additional file : Fig. S9a).

Marker:

Article Title: Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis
Article Snippet: .. Development of novel reporter construct To create a novel transposon from which the antibiotic resistance marker could be excised following transposon insertion in, and disruption of, a specific gene, operator and gene region fragments were designed and then synthesised commercially and cloned in the Eco RV site of pUC57 (GenScript Corporation). .. The operator region contained an inverted repeat (IR) (39 bp, 5′-gataaagtccgtataattgtgtaaaagtaaaaaggccat-3′) together with the M. bovis tuf promoter (252 bp tuf promoter region located between bases 474270 and 474521 of NCBI Reference Sequence NC_014760.1), a vsp signal sequence (84 bp, gene ID 10014768, predicted protein sequence MKKSKFLLLGSVASLASIPFVAAKCGET ) and the FRT sequence (34 bp Flp recognition target, 5′-gaagttcctattctctagaaagtataggaacttc-3′ ).

Plasmid Preparation:

Article Title: Characterization of the Salmonella enterica Serovar Typhimurium ydcI Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ Gene, Which Encodes a Conserved DNA Binding Protein Required for Full Acid Stress Resistance ▿ †
Article Snippet: .. Plasmid pBAD18+ ydcI was constructed using a synthesized ydcI gene in which a six-histidine tag was fused to the C terminus and cloned into pUC57 ( ) and then subcloned into pBAD18 ( ) downstream of the arabinose-inducible promoter (Genscript, Inc., Piscataway, NJ). ..

Article Title: Creation of a Nonspreading Rift Valley Fever Virus ▿
Article Snippet: .. The consensus sequences corresponding to each genome segment were synthesized and cloned in pUC57, a standard cloning vector of the GenScript Corporation (Piscataway, NJ). pUC57-L, pUC57-M, and pUC57-S encode the RVFV L, M, and S genome segments, respectively, in antigenomic orientation. .. The complete sequence of the L, M, and S genome sequences can be found in GenBank under the accession numbers , , and , respectively.

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  • 92
    GenScript puc57 cry5ba3φ
    Recombination of <t>cry5Ba3Φ</t> -transgenic Botrytis cinerea . a Construction of the plasmid pTFCM- cry5Ba3Φ . HYG: hygromycin B resistant (hygromycin B phosphotransferase) gene; PtrpC and TtrpC : promoter and terminator of Aspergillus nidulans , respectively. b Plasmid <t>pUC57-</t> cry5Ba3Φ digested by Spe I and Xho I. M: DNA marker; 1: plasmid pUC57; 2: plasmid pUC57- cry5Ba3Φ . c Certification of the plasmid pTFCM- cry5Ba3Φ . M: DNA marker; 1–2: plasmid pTFCM- cry5Ba3Φ digested by Sac I and Xho I; 3–4: plasmid pTFCM- cry5Ba3Φ digested by Spe I and Xho I. d Identification of the AGL-1 pTFCM- cry5Ba3Φ by PCR amplification with cry-F/cry-R primers. M: DNA Marker; 1: plasmid pTFCM- cry5Ba3Φ ; 2–4: AGL-1 pTFCM- cry5Ba3Φ ; 5: AGL-1 pTFCM. e Southern blot analysis of genomic DNA of cry5Ba3Φ -transgenic Botrytis cinerea . DNA was digested with Hin dIII and probed with cry5Ba3Φ . 1: cry5Ba3Φ -transgenic Botrytis cinerea ; 2: wild-type Botrytis cinerea ; 3: plasmid pTFCM- cry5Ba3Φ . f Confirmation of cry5Ba3Φ -transgenic Botrytis cinerea by PCR amplification with cry-F/cry-R primers. M: DNA marker; 1: cry5Ba3Φ -transgenic Botrytis cinerea ; 2: plasmid pTFCM- cry5Ba3Φ ; 3: wild-type Botrytis cinerea . g Expression quantity of cry5Ba3Φ gene in cry5Ba3Φ -transgenic and wild-type Botrytis cinerea strains ( P
    Puc57 Cry5ba3φ, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc57 cry5ba3φ/product/GenScript
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    puc57 cry5ba3φ - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    GenScript puc57 vector
    Rad23b and Ddit3 methylation determined by BSP. Bisulfite treated DNA was amplified by PCR with BSP primers. PCR products were cloned into the <t>pUC57</t> vector, and five clones selected and sequenced from each sample. Methylation level was defined as the ratio of methylated CpG sites in all clones. (A) Typical sequencing results; (B) early effects, tissues were collected 2 h postirradiation; (C) delay effects, 1 month postirradiation. * P
    Puc57 Vector, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc57 vector/product/GenScript
    Average 92 stars, based on 207 article reviews
    Price from $9.99 to $1999.99
    puc57 vector - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    GenScript puc57
    The lncRNA CHRF promotes silica-induced pulmonary fibrosis through targeting miR-489. (A) Western blot analysis of MyD88, IL-1β and TGF-β1 expression in RAW 264.7 cells and (B) total Smad3 and p-Smad3 expression in NIH/3T3 cells transfected with <t>pUC57</t> plasmids of CHRF with * P
    Puc57, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc57/product/GenScript
    Average 93 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    puc57 - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    92
    GenScript puc57 nras f rna vector
    ZnAPC decreases the amount of NRAS mRNA. a Denaturing polyacrylamide gel electrophoresis (10% gel) of 0.1 µM NRAS <t>RNA</t> or dsRNA in the presence of 2 µM ZnAPC, FeAPC, NiAPC or CuAPC after photo-irradiation for the indicated periods at 25 °C. b Residual intact RNA after photo-irradiation of NRAS RNA and dsRNA in the presence of APCs. Error bars represent mean ± SD; n = 3. c 0.1 µM NRAS F-RNA labelled with DIG in the presence of 2 µM ZnAPC before and after photo-irradiation for 120 min were analysed by electrophoresis in a 15% denaturing polyacrylamide gel. The RNA was detected with antibody against DIG. d , e Cells pre-treated with 10 µM ZnAPC or FeAPC together with 1 µg ml −1 actinomycin D, which inhibits de novo mRNA synthesis, for 1 h were photo-irradiated for 2 h. d The amount of NRAS mRNA was evaluated by real-time PCR. Each bar represents mean ± SD; n = 3. For statistical significance, an unpaired t -test was performed. *** p
    Puc57 Nras F Rna Vector, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc57 nras f rna vector/product/GenScript
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    puc57 nras f rna vector - by Bioz Stars, 2020-09
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    Recombination of cry5Ba3Φ -transgenic Botrytis cinerea . a Construction of the plasmid pTFCM- cry5Ba3Φ . HYG: hygromycin B resistant (hygromycin B phosphotransferase) gene; PtrpC and TtrpC : promoter and terminator of Aspergillus nidulans , respectively. b Plasmid pUC57- cry5Ba3Φ digested by Spe I and Xho I. M: DNA marker; 1: plasmid pUC57; 2: plasmid pUC57- cry5Ba3Φ . c Certification of the plasmid pTFCM- cry5Ba3Φ . M: DNA marker; 1–2: plasmid pTFCM- cry5Ba3Φ digested by Sac I and Xho I; 3–4: plasmid pTFCM- cry5Ba3Φ digested by Spe I and Xho I. d Identification of the AGL-1 pTFCM- cry5Ba3Φ by PCR amplification with cry-F/cry-R primers. M: DNA Marker; 1: plasmid pTFCM- cry5Ba3Φ ; 2–4: AGL-1 pTFCM- cry5Ba3Φ ; 5: AGL-1 pTFCM. e Southern blot analysis of genomic DNA of cry5Ba3Φ -transgenic Botrytis cinerea . DNA was digested with Hin dIII and probed with cry5Ba3Φ . 1: cry5Ba3Φ -transgenic Botrytis cinerea ; 2: wild-type Botrytis cinerea ; 3: plasmid pTFCM- cry5Ba3Φ . f Confirmation of cry5Ba3Φ -transgenic Botrytis cinerea by PCR amplification with cry-F/cry-R primers. M: DNA marker; 1: cry5Ba3Φ -transgenic Botrytis cinerea ; 2: plasmid pTFCM- cry5Ba3Φ ; 3: wild-type Botrytis cinerea . g Expression quantity of cry5Ba3Φ gene in cry5Ba3Φ -transgenic and wild-type Botrytis cinerea strains ( P

    Journal: Microbial Cell Factories

    Article Title: Suppressing a plant-parasitic nematode with fungivorous behavior by fungal transformation of a Bt cry gene

    doi: 10.1186/s12934-018-0960-5

    Figure Lengend Snippet: Recombination of cry5Ba3Φ -transgenic Botrytis cinerea . a Construction of the plasmid pTFCM- cry5Ba3Φ . HYG: hygromycin B resistant (hygromycin B phosphotransferase) gene; PtrpC and TtrpC : promoter and terminator of Aspergillus nidulans , respectively. b Plasmid pUC57- cry5Ba3Φ digested by Spe I and Xho I. M: DNA marker; 1: plasmid pUC57; 2: plasmid pUC57- cry5Ba3Φ . c Certification of the plasmid pTFCM- cry5Ba3Φ . M: DNA marker; 1–2: plasmid pTFCM- cry5Ba3Φ digested by Sac I and Xho I; 3–4: plasmid pTFCM- cry5Ba3Φ digested by Spe I and Xho I. d Identification of the AGL-1 pTFCM- cry5Ba3Φ by PCR amplification with cry-F/cry-R primers. M: DNA Marker; 1: plasmid pTFCM- cry5Ba3Φ ; 2–4: AGL-1 pTFCM- cry5Ba3Φ ; 5: AGL-1 pTFCM. e Southern blot analysis of genomic DNA of cry5Ba3Φ -transgenic Botrytis cinerea . DNA was digested with Hin dIII and probed with cry5Ba3Φ . 1: cry5Ba3Φ -transgenic Botrytis cinerea ; 2: wild-type Botrytis cinerea ; 3: plasmid pTFCM- cry5Ba3Φ . f Confirmation of cry5Ba3Φ -transgenic Botrytis cinerea by PCR amplification with cry-F/cry-R primers. M: DNA marker; 1: cry5Ba3Φ -transgenic Botrytis cinerea ; 2: plasmid pTFCM- cry5Ba3Φ ; 3: wild-type Botrytis cinerea . g Expression quantity of cry5Ba3Φ gene in cry5Ba3Φ -transgenic and wild-type Botrytis cinerea strains ( P

    Article Snippet: The designed cry5Ba3Φ was then synthesized, combined with trpC promoter/terminator and sticky ends Xho I/Spe I, and linked with a pUC57 plasmid to obtain pUC57-cry5Ba3Φ (Genscript Co. Ltd., Nanjing, Jiangsu Province, China) (Fig. a).

    Techniques: Transgenic Assay, Plasmid Preparation, Marker, Polymerase Chain Reaction, Amplification, Southern Blot, Expressing

    Rad23b and Ddit3 methylation determined by BSP. Bisulfite treated DNA was amplified by PCR with BSP primers. PCR products were cloned into the pUC57 vector, and five clones selected and sequenced from each sample. Methylation level was defined as the ratio of methylated CpG sites in all clones. (A) Typical sequencing results; (B) early effects, tissues were collected 2 h postirradiation; (C) delay effects, 1 month postirradiation. * P

    Journal: PLoS ONE

    Article Title: Genome-Wide Screen of DNA Methylation Changes Induced by Low Dose X-Ray Radiation in Mice

    doi: 10.1371/journal.pone.0090804

    Figure Lengend Snippet: Rad23b and Ddit3 methylation determined by BSP. Bisulfite treated DNA was amplified by PCR with BSP primers. PCR products were cloned into the pUC57 vector, and five clones selected and sequenced from each sample. Methylation level was defined as the ratio of methylated CpG sites in all clones. (A) Typical sequencing results; (B) early effects, tissues were collected 2 h postirradiation; (C) delay effects, 1 month postirradiation. * P

    Article Snippet: PCR products were cloned into the pUC57 vector (Genscript, Nanjing, China), and five clones selected and sequenced from each sample.

    Techniques: Methylation, Amplification, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation, Sequencing

    The lncRNA CHRF promotes silica-induced pulmonary fibrosis through targeting miR-489. (A) Western blot analysis of MyD88, IL-1β and TGF-β1 expression in RAW 264.7 cells and (B) total Smad3 and p-Smad3 expression in NIH/3T3 cells transfected with pUC57 plasmids of CHRF with * P

    Journal: Scientific Reports

    Article Title: miR-489 inhibits silica-induced pulmonary fibrosis by targeting MyD88 and Smad3 and is negatively regulated by lncRNA CHRF

    doi: 10.1038/srep30921

    Figure Lengend Snippet: The lncRNA CHRF promotes silica-induced pulmonary fibrosis through targeting miR-489. (A) Western blot analysis of MyD88, IL-1β and TGF-β1 expression in RAW 264.7 cells and (B) total Smad3 and p-Smad3 expression in NIH/3T3 cells transfected with pUC57 plasmids of CHRF with * P

    Article Snippet: Plasmids construction and cell transfection The CHRF lncRNA sequence was synthesized and subcloned into pUC57 to generate CHRF plasmids (GenScript, Nanjing, China).

    Techniques: Western Blot, Expressing, Transfection

    ZnAPC decreases the amount of NRAS mRNA. a Denaturing polyacrylamide gel electrophoresis (10% gel) of 0.1 µM NRAS RNA or dsRNA in the presence of 2 µM ZnAPC, FeAPC, NiAPC or CuAPC after photo-irradiation for the indicated periods at 25 °C. b Residual intact RNA after photo-irradiation of NRAS RNA and dsRNA in the presence of APCs. Error bars represent mean ± SD; n = 3. c 0.1 µM NRAS F-RNA labelled with DIG in the presence of 2 µM ZnAPC before and after photo-irradiation for 120 min were analysed by electrophoresis in a 15% denaturing polyacrylamide gel. The RNA was detected with antibody against DIG. d , e Cells pre-treated with 10 µM ZnAPC or FeAPC together with 1 µg ml −1 actinomycin D, which inhibits de novo mRNA synthesis, for 1 h were photo-irradiated for 2 h. d The amount of NRAS mRNA was evaluated by real-time PCR. Each bar represents mean ± SD; n = 3. For statistical significance, an unpaired t -test was performed. *** p

    Journal: Nature Communications

    Article Title: An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex

    doi: 10.1038/s41467-018-04771-y

    Figure Lengend Snippet: ZnAPC decreases the amount of NRAS mRNA. a Denaturing polyacrylamide gel electrophoresis (10% gel) of 0.1 µM NRAS RNA or dsRNA in the presence of 2 µM ZnAPC, FeAPC, NiAPC or CuAPC after photo-irradiation for the indicated periods at 25 °C. b Residual intact RNA after photo-irradiation of NRAS RNA and dsRNA in the presence of APCs. Error bars represent mean ± SD; n = 3. c 0.1 µM NRAS F-RNA labelled with DIG in the presence of 2 µM ZnAPC before and after photo-irradiation for 120 min were analysed by electrophoresis in a 15% denaturing polyacrylamide gel. The RNA was detected with antibody against DIG. d , e Cells pre-treated with 10 µM ZnAPC or FeAPC together with 1 µg ml −1 actinomycin D, which inhibits de novo mRNA synthesis, for 1 h were photo-irradiated for 2 h. d The amount of NRAS mRNA was evaluated by real-time PCR. Each bar represents mean ± SD; n = 3. For statistical significance, an unpaired t -test was performed. *** p

    Article Snippet: Cleavage of NRAS F-RNA The pUC57 NRAS F-RNA vector containing the sequence encoding the 5′ UTR of NRAS mRNA following T7-promoter was constructed (GenScript Japan Inc.).

    Techniques: Polyacrylamide Gel Electrophoresis, Irradiation, Electrophoresis, Real-time Polymerase Chain Reaction