puc19 vector dna  (New England Biolabs)


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    Name:
    pUC19 Vector
    Description:
    pUC19 Vector 250 ug
    Catalog Number:
    N3041L
    Price:
    300
    Category:
    Vectors Plasmids
    Size:
    250 ug
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    Structured Review

    New England Biolabs puc19 vector dna
    pUC19 Vector
    pUC19 Vector 250 ug
    https://www.bioz.com/result/puc19 vector dna/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    puc19 vector dna - by Bioz Stars, 2021-05
    86/100 stars

    Images

    1) Product Images from "Synthesis of the unnatural amino acid Nα-Nε-(ferrocene-1-acetyl)-l-lysine: a novel organometallic nuclease"

    Article Title: Synthesis of the unnatural amino acid Nα-Nε-(ferrocene-1-acetyl)-l-lysine: a novel organometallic nuclease

    Journal: Journal of organometallic chemistry

    doi: 10.1016/j.jorganchem.2008.06.012

    DNA cleavage assays. A 20 μl reaction containing pUC19 DNA (1.0 μg, 75 μM bp) was incubated with 0-150 μM of 1 (a) or ferrocene acetic acid (b) at 25 °C for 16 h. Separation of supercoiled (SC), nicked circular (NC), and linear (L) forms of pUC19 DNA in each reaction was accomplished by agarose (1%) gel electrophoresis.
    Figure Legend Snippet: DNA cleavage assays. A 20 μl reaction containing pUC19 DNA (1.0 μg, 75 μM bp) was incubated with 0-150 μM of 1 (a) or ferrocene acetic acid (b) at 25 °C for 16 h. Separation of supercoiled (SC), nicked circular (NC), and linear (L) forms of pUC19 DNA in each reaction was accomplished by agarose (1%) gel electrophoresis.

    Techniques Used: Incubation, Nucleic Acid Electrophoresis

    2) Product Images from "Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing"

    Article Title: Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkv738

    Data analysis of spike-in controls from MethylC-seq and 4mC-TAB-seq in the context of C. kristjanssonii genomic DNA. ( A ) Composition of pUC19 DNA, lambda DNA, and C. kristjanssonii genomic DNA. ( B ) The percentage of detected as cytosine reads on 4mC sites in untreated and Tet-treated samples. ( C ) The detected as cytosine reads percentage on unmodified cytosine sites (non-CpG context) and 5mC sites (CpG context) in untreated and Tet-treated samples. ( D ) Quantification of 4mC and 5mC in C. kristjanssonii genomic DNA, determined by LC-MS/MS and deep-sequencing respectively. Error bars indicate mean ± SD, n = 4.
    Figure Legend Snippet: Data analysis of spike-in controls from MethylC-seq and 4mC-TAB-seq in the context of C. kristjanssonii genomic DNA. ( A ) Composition of pUC19 DNA, lambda DNA, and C. kristjanssonii genomic DNA. ( B ) The percentage of detected as cytosine reads on 4mC sites in untreated and Tet-treated samples. ( C ) The detected as cytosine reads percentage on unmodified cytosine sites (non-CpG context) and 5mC sites (CpG context) in untreated and Tet-treated samples. ( D ) Quantification of 4mC and 5mC in C. kristjanssonii genomic DNA, determined by LC-MS/MS and deep-sequencing respectively. Error bars indicate mean ± SD, n = 4.

    Techniques Used: Lambda DNA Preparation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Sequencing

    Related Articles

    Mutagenesis:

    Article Title: Characterization of Two Seryl-tRNA Synthetases in Albomycin-Producing Streptomyces sp. Strain ATCC 700974 ▿
    Article Snippet: Genetic complementation was used to test the in vivo activities of SerRS1 and SerRS2. .. The host strain was an E. coli mutant (K28) that is ineffective at making its own serS gene product at high temperature ( , ). serS1 and serS2 were separately cloned into a high-copy vector, pUC19, under the control of a constitutive glnS ′ promoter, which is a glnS mutant promoter used for aaRS expression in E. coli , and the resulting plasmids were transformed into E. coli K28. ..

    Clone Assay:

    Article Title: Characterization of Two Seryl-tRNA Synthetases in Albomycin-Producing Streptomyces sp. Strain ATCC 700974 ▿
    Article Snippet: Genetic complementation was used to test the in vivo activities of SerRS1 and SerRS2. .. The host strain was an E. coli mutant (K28) that is ineffective at making its own serS gene product at high temperature ( , ). serS1 and serS2 were separately cloned into a high-copy vector, pUC19, under the control of a constitutive glnS ′ promoter, which is a glnS mutant promoter used for aaRS expression in E. coli , and the resulting plasmids were transformed into E. coli K28. ..

    Article Title: Nucleosome Spacing Generated by ISWI and CHD1 Remodelers Is Constant Regardless of Nucleosome Density
    Article Snippet: .. Plasmids were isolated from Escherichia coli using the PureYield Maxiprep system (Promega). pUC19-PHO8 contains ∼3.5 kb of the Saccharomyces cerevisiae PHO8 locus cloned into pUC19 via BamHI and PstI, is 6,168 bp long, and is described as “pUC19-PHO8-long” in reference . pUC19-GCY1 contains ∼3.5 kb of the S. cerevisiae GCY1 locus (PCR product using primers 5′-CAGTCGGATGGAGCTCACTTCTATTGGCTTAGGAGC-3′ and 5′-CACTGTGCATTTCTAGAACGACGAAGACGAGGATTAG-3′ and genomic DNA of strain BY4741 [EUROSCARF] as the template) cloned into pUC19 via SacI and XbaI. pUC19-PHO8 and pUC19-GCY1 were linearized with BamHI (NEB), which cleaves right at or 400 bp downstream of, respectively, the upstream border between prokaryotic and eukaryotic DNA. .. The complete plasmids were used as the template for salt gradient dialysis (SGD) reconstitution, and complete linearization was confirmed by agarose gel electrophoresis prior to chromatin assembly.

    Article Title: Characterization of Two Seryl-tRNA Synthetases in Albomycin-Producing Streptomyces sp. Strain ATCC 700974 ▿
    Article Snippet: The E. coli serS gene, including its native promoter and terminator, was amplified from E. coli JM109 genomic DNA using primers EcserS-F and EcserS-R. .. The product was cloned into the XbaI and EcoRI sites of pUC19 to produce pUC-EcSerRS. ..

    Plasmid Preparation:

    Article Title: Characterization of Two Seryl-tRNA Synthetases in Albomycin-Producing Streptomyces sp. Strain ATCC 700974 ▿
    Article Snippet: Genetic complementation was used to test the in vivo activities of SerRS1 and SerRS2. .. The host strain was an E. coli mutant (K28) that is ineffective at making its own serS gene product at high temperature ( , ). serS1 and serS2 were separately cloned into a high-copy vector, pUC19, under the control of a constitutive glnS ′ promoter, which is a glnS mutant promoter used for aaRS expression in E. coli , and the resulting plasmids were transformed into E. coli K28. ..

    Article Title: Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing
    Article Snippet: Genomic DNA concentrations were determined using the Qubit® 2.0 fluorometer (Invitrogen) and the quality of DNA was assessed by agarose gel electrophoresis. .. Preparation of 304 bp model DNA with 4mC modifications For N 4 -methylcytosine (4mC) containing model DNA, 0.5 ng of pUC19 vector DNA (NEB) was PCR amplified as follows in a 50 μl reaction: 2.5 U RedTaq polymerase (Sigma), 5 μl 10× reaction buffer, 1 μl N 4 -methyl-dCTP (4mdCTP) (Trilink)/dATP/dGTP/dTTP cocktail (10 mM each), 1 μl 10 mM forward primer (5′-GAACGAAAACTCACGTTAAGGG), 1 μl 10 mM reverse primer (5′-TGCTGATAAATCTGGAGCCG). ..

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents
    Article Snippet: Since condensation by C 3 opioids had been established by viscosity and turbidity measurements, visualization of DNA compaction was then followed by electrophoresis. .. Samples were titrated against both supercoiled pUC19 plasmid DNA and a 742 bp dsDNA fragment amplified from pUC19 encompassing the lacZα gene, before incubation for 5 h prior to analysis by agarose gel electrophoresis (Figure ). ..

    Expressing:

    Article Title: Characterization of Two Seryl-tRNA Synthetases in Albomycin-Producing Streptomyces sp. Strain ATCC 700974 ▿
    Article Snippet: Genetic complementation was used to test the in vivo activities of SerRS1 and SerRS2. .. The host strain was an E. coli mutant (K28) that is ineffective at making its own serS gene product at high temperature ( , ). serS1 and serS2 were separately cloned into a high-copy vector, pUC19, under the control of a constitutive glnS ′ promoter, which is a glnS mutant promoter used for aaRS expression in E. coli , and the resulting plasmids were transformed into E. coli K28. ..

    Transformation Assay:

    Article Title: Characterization of Two Seryl-tRNA Synthetases in Albomycin-Producing Streptomyces sp. Strain ATCC 700974 ▿
    Article Snippet: Genetic complementation was used to test the in vivo activities of SerRS1 and SerRS2. .. The host strain was an E. coli mutant (K28) that is ineffective at making its own serS gene product at high temperature ( , ). serS1 and serS2 were separately cloned into a high-copy vector, pUC19, under the control of a constitutive glnS ′ promoter, which is a glnS mutant promoter used for aaRS expression in E. coli , and the resulting plasmids were transformed into E. coli K28. ..

    Article Title: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct
    Article Snippet: After 4 h of incubation at 37°C, the amount of TNF-α released in the medium was measured in duplicate by ELISA (R & D Systems) according to the manufacturer’s instructions. .. Periplasmic fraction of pUC19 transformed E. coli BL21 (DE3) was used as TNF-α induction control. .. Commercial EBV IL-10 (R & D Systems) served as positive control.

    Isolation:

    Article Title: Nucleosome Spacing Generated by ISWI and CHD1 Remodelers Is Constant Regardless of Nucleosome Density
    Article Snippet: .. Plasmids were isolated from Escherichia coli using the PureYield Maxiprep system (Promega). pUC19-PHO8 contains ∼3.5 kb of the Saccharomyces cerevisiae PHO8 locus cloned into pUC19 via BamHI and PstI, is 6,168 bp long, and is described as “pUC19-PHO8-long” in reference . pUC19-GCY1 contains ∼3.5 kb of the S. cerevisiae GCY1 locus (PCR product using primers 5′-CAGTCGGATGGAGCTCACTTCTATTGGCTTAGGAGC-3′ and 5′-CACTGTGCATTTCTAGAACGACGAAGACGAGGATTAG-3′ and genomic DNA of strain BY4741 [EUROSCARF] as the template) cloned into pUC19 via SacI and XbaI. pUC19-PHO8 and pUC19-GCY1 were linearized with BamHI (NEB), which cleaves right at or 400 bp downstream of, respectively, the upstream border between prokaryotic and eukaryotic DNA. .. The complete plasmids were used as the template for salt gradient dialysis (SGD) reconstitution, and complete linearization was confirmed by agarose gel electrophoresis prior to chromatin assembly.

    Polymerase Chain Reaction:

    Article Title: Nucleosome Spacing Generated by ISWI and CHD1 Remodelers Is Constant Regardless of Nucleosome Density
    Article Snippet: .. Plasmids were isolated from Escherichia coli using the PureYield Maxiprep system (Promega). pUC19-PHO8 contains ∼3.5 kb of the Saccharomyces cerevisiae PHO8 locus cloned into pUC19 via BamHI and PstI, is 6,168 bp long, and is described as “pUC19-PHO8-long” in reference . pUC19-GCY1 contains ∼3.5 kb of the S. cerevisiae GCY1 locus (PCR product using primers 5′-CAGTCGGATGGAGCTCACTTCTATTGGCTTAGGAGC-3′ and 5′-CACTGTGCATTTCTAGAACGACGAAGACGAGGATTAG-3′ and genomic DNA of strain BY4741 [EUROSCARF] as the template) cloned into pUC19 via SacI and XbaI. pUC19-PHO8 and pUC19-GCY1 were linearized with BamHI (NEB), which cleaves right at or 400 bp downstream of, respectively, the upstream border between prokaryotic and eukaryotic DNA. .. The complete plasmids were used as the template for salt gradient dialysis (SGD) reconstitution, and complete linearization was confirmed by agarose gel electrophoresis prior to chromatin assembly.

    Article Title: Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing
    Article Snippet: Genomic DNA concentrations were determined using the Qubit® 2.0 fluorometer (Invitrogen) and the quality of DNA was assessed by agarose gel electrophoresis. .. Preparation of 304 bp model DNA with 4mC modifications For N 4 -methylcytosine (4mC) containing model DNA, 0.5 ng of pUC19 vector DNA (NEB) was PCR amplified as follows in a 50 μl reaction: 2.5 U RedTaq polymerase (Sigma), 5 μl 10× reaction buffer, 1 μl N 4 -methyl-dCTP (4mdCTP) (Trilink)/dATP/dGTP/dTTP cocktail (10 mM each), 1 μl 10 mM forward primer (5′-GAACGAAAACTCACGTTAAGGG), 1 μl 10 mM reverse primer (5′-TGCTGATAAATCTGGAGCCG). ..

    Amplification:

    Article Title: Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing
    Article Snippet: Genomic DNA concentrations were determined using the Qubit® 2.0 fluorometer (Invitrogen) and the quality of DNA was assessed by agarose gel electrophoresis. .. Preparation of 304 bp model DNA with 4mC modifications For N 4 -methylcytosine (4mC) containing model DNA, 0.5 ng of pUC19 vector DNA (NEB) was PCR amplified as follows in a 50 μl reaction: 2.5 U RedTaq polymerase (Sigma), 5 μl 10× reaction buffer, 1 μl N 4 -methyl-dCTP (4mdCTP) (Trilink)/dATP/dGTP/dTTP cocktail (10 mM each), 1 μl 10 mM forward primer (5′-GAACGAAAACTCACGTTAAGGG), 1 μl 10 mM reverse primer (5′-TGCTGATAAATCTGGAGCCG). ..

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents
    Article Snippet: Since condensation by C 3 opioids had been established by viscosity and turbidity measurements, visualization of DNA compaction was then followed by electrophoresis. .. Samples were titrated against both supercoiled pUC19 plasmid DNA and a 742 bp dsDNA fragment amplified from pUC19 encompassing the lacZα gene, before incubation for 5 h prior to analysis by agarose gel electrophoresis (Figure ). ..

    Incubation:

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents
    Article Snippet: Since condensation by C 3 opioids had been established by viscosity and turbidity measurements, visualization of DNA compaction was then followed by electrophoresis. .. Samples were titrated against both supercoiled pUC19 plasmid DNA and a 742 bp dsDNA fragment amplified from pUC19 encompassing the lacZα gene, before incubation for 5 h prior to analysis by agarose gel electrophoresis (Figure ). ..

    Agarose Gel Electrophoresis:

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents
    Article Snippet: Since condensation by C 3 opioids had been established by viscosity and turbidity measurements, visualization of DNA compaction was then followed by electrophoresis. .. Samples were titrated against both supercoiled pUC19 plasmid DNA and a 742 bp dsDNA fragment amplified from pUC19 encompassing the lacZα gene, before incubation for 5 h prior to analysis by agarose gel electrophoresis (Figure ). ..

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  • 99
    New England Biolabs puc19 vector dna
    Data analysis of spike-in controls from MethylC-seq and 4mC-TAB-seq in the context of C. kristjanssonii genomic <t>DNA.</t> ( A ) Composition of <t>pUC19</t> DNA, lambda DNA, and C. kristjanssonii genomic DNA. ( B ) The percentage of detected as cytosine reads on 4mC sites in untreated and Tet-treated samples. ( C ) The detected as cytosine reads percentage on unmodified cytosine sites (non-CpG context) and 5mC sites (CpG context) in untreated and Tet-treated samples. ( D ) Quantification of 4mC and 5mC in C. kristjanssonii genomic DNA, determined by LC-MS/MS and deep-sequencing respectively. Error bars indicate mean ± SD, n = 4.
    Puc19 Vector Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc19 vector dna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    puc19 vector dna - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

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    Data analysis of spike-in controls from MethylC-seq and 4mC-TAB-seq in the context of C. kristjanssonii genomic DNA. ( A ) Composition of pUC19 DNA, lambda DNA, and C. kristjanssonii genomic DNA. ( B ) The percentage of detected as cytosine reads on 4mC sites in untreated and Tet-treated samples. ( C ) The detected as cytosine reads percentage on unmodified cytosine sites (non-CpG context) and 5mC sites (CpG context) in untreated and Tet-treated samples. ( D ) Quantification of 4mC and 5mC in C. kristjanssonii genomic DNA, determined by LC-MS/MS and deep-sequencing respectively. Error bars indicate mean ± SD, n = 4.

    Journal: Nucleic Acids Research

    Article Title: Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing

    doi: 10.1093/nar/gkv738

    Figure Lengend Snippet: Data analysis of spike-in controls from MethylC-seq and 4mC-TAB-seq in the context of C. kristjanssonii genomic DNA. ( A ) Composition of pUC19 DNA, lambda DNA, and C. kristjanssonii genomic DNA. ( B ) The percentage of detected as cytosine reads on 4mC sites in untreated and Tet-treated samples. ( C ) The detected as cytosine reads percentage on unmodified cytosine sites (non-CpG context) and 5mC sites (CpG context) in untreated and Tet-treated samples. ( D ) Quantification of 4mC and 5mC in C. kristjanssonii genomic DNA, determined by LC-MS/MS and deep-sequencing respectively. Error bars indicate mean ± SD, n = 4.

    Article Snippet: Preparation of 304 bp model DNA with 4mC modifications For N 4 -methylcytosine (4mC) containing model DNA, 0.5 ng of pUC19 vector DNA (NEB) was PCR amplified as follows in a 50 μl reaction: 2.5 U RedTaq polymerase (Sigma), 5 μl 10× reaction buffer, 1 μl N 4 -methyl-dCTP (4mdCTP) (Trilink)/dATP/dGTP/dTTP cocktail (10 mM each), 1 μl 10 mM forward primer (5′-GAACGAAAACTCACGTTAAGGG), 1 μl 10 mM reverse primer (5′-TGCTGATAAATCTGGAGCCG).

    Techniques: Lambda DNA Preparation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Sequencing

    Lys680 mutants transfer less UvrB onto the damaged oligonucleotide than the other UvrA variants Panel a , The ability of the individual UvrA proteins to load WT UvrB onto the radiolabeled fluorescein adducted oligonucleotide was monitored by EMSA. The UvrA proteins (20 nM) were incubated with UvrB (100 nM) with or without competitor DNA (pUC19) for 15 min at 55 °C. The protein-DNA complexes were separated on 3.5% native polyacrylamide gels containing ATP (1 mM) and MgCl 2 (10 mM). Dashed lines indicate merged gels run at the same time. pUC is the plasmid, pUC19. Panel b . Analysis of the EMSAs reporting the percentage of DNA in the B·DNA complex. White and filled bars represent B·DNA complexes produced in the absence and presence of competitor DNA, respectively. The data are reported as the mean ± S.D., n=3. Paired Student's T-tests were performed between WT and the variants and (*) indicates that the probability was less than 0.05 while (**) reflects a probability less than 0.01.

    Journal: DNA repair

    Article Title: Cooperative damage recognition by UvrA and UvrB: Identification of UvrA residues that mediate DNA binding

    doi: 10.1016/j.dnarep.2007.11.013

    Figure Lengend Snippet: Lys680 mutants transfer less UvrB onto the damaged oligonucleotide than the other UvrA variants Panel a , The ability of the individual UvrA proteins to load WT UvrB onto the radiolabeled fluorescein adducted oligonucleotide was monitored by EMSA. The UvrA proteins (20 nM) were incubated with UvrB (100 nM) with or without competitor DNA (pUC19) for 15 min at 55 °C. The protein-DNA complexes were separated on 3.5% native polyacrylamide gels containing ATP (1 mM) and MgCl 2 (10 mM). Dashed lines indicate merged gels run at the same time. pUC is the plasmid, pUC19. Panel b . Analysis of the EMSAs reporting the percentage of DNA in the B·DNA complex. White and filled bars represent B·DNA complexes produced in the absence and presence of competitor DNA, respectively. The data are reported as the mean ± S.D., n=3. Paired Student's T-tests were performed between WT and the variants and (*) indicates that the probability was less than 0.05 while (**) reflects a probability less than 0.01.

    Article Snippet: For those reactions containing competitor DNA, 309 ng pUC19 DNA (New England Biolabs) was added.

    Techniques: Incubation, Plasmid Preparation, Produced

    Effect of MarR on gyrase activity. The mix constituents are 0.5 μg of pUC19 relaxed, 1 unit (120 pmol) of purified E. coli DNA gyrase (4.1 μM) and 250 pmol (8 μg) of purified MarR (8.3 μM). Samples were incubated at 37°C for 30 min, subjected to electrophoresis in a 0.8% agarose gel, and stained after migration by ethidium bromide (see Materials and Methods).

    Journal: Journal of Bacteriology

    Article Title: GyrA Interacts with MarR To Reduce Repression of the marRAB Operon in Escherichia coli ▿

    doi: 10.1128/JB.01259-09

    Figure Lengend Snippet: Effect of MarR on gyrase activity. The mix constituents are 0.5 μg of pUC19 relaxed, 1 unit (120 pmol) of purified E. coli DNA gyrase (4.1 μM) and 250 pmol (8 μg) of purified MarR (8.3 μM). Samples were incubated at 37°C for 30 min, subjected to electrophoresis in a 0.8% agarose gel, and stained after migration by ethidium bromide (see Materials and Methods).

    Article Snippet: One unit (120 pmol) of E. coli DNA gyrase and 0.5 μg of pUC19 DNA relaxed by topoisomerase I (both from New England Biolabs) were mixed with or without 250 pmol MarR in 30 μl reaction buffer containing 35 mM Tris-HCl, 24 mM KCl, 4 mM MgCl2, 2 mM dithiothreitol (DTT), 1.75 mM ATP, 5 mM spermidine, 0.1 mg/ml bovine serum albumin, 6.5% glycerol at pH 7.5.

    Techniques: Activity Assay, Purification, Incubation, Electrophoresis, Agarose Gel Electrophoresis, Staining, Migration