histone h1 phosphoinositides shuttle carrier  (Echelon Biosciences)

Journal: World Journal of Gastroenterology

Article Title: RGS4 promotes the progression of gastric cancer through the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial-mesenchymal transition

doi: 10.3748/wjg.v31.i2.100898

Figure Lengend Snippet: The expression level of regulator of G protein signaling 4 affected the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial mesenchymal transition in gastric cancer cells. A: Gene Set Enrichment Analysis results showed the relevant pathways that regulator of G protein signaling 4 may affected in The Cancer Genome Atlas Stomach Cancer dataset; B-E: The expression levels of focal adhesion kinase signaling pathway and epithelial mesenchymal transition related protein of MGC-803 cells and AGS cells were determined by western blot analysis. β-actin was used as a loading control. b P < 0.01, c P < 0.001, d P < 0.0001. FAK: Focal adhesion kinase; PI3K: Phosphatidyl-inositol-3-kinase; AKT: Protein kinase B.

Article Snippet: Next, primary antibodies [ RGS4 , ab308160, 1:1500, Abcam; focal adhesion kinase (FAK), ab40794, 1:2000, Abcam; p-FAK, ab81298, 1:1500, Abcam; phosphatidyl-inositol-3-kinase (PI3K), ab302958, 1:1500, Abcam; p-PI3K, ab182651, 1:200, Abcam; protein kinase B (AKT), ab8805, 1:500, Abcam; p-AKT, ab38449, 1:1500, Abcam; E-cadherin, ab40772, 1:1500, Abcam; N-cadherin, ab76011, 1:5000, Abcam; vimentin, ab20346, 1:1500, Abcam; β-actin, ab8227, 1:2000, Abcam] were used to nurture membranes.

Techniques: Expressing, Western Blot, Control

Journal: World Journal of Gastroenterology

Article Title: RGS4 promotes the progression of gastric cancer through the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial-mesenchymal transition

doi: 10.3748/wjg.v31.i2.100898

Figure Lengend Snippet: Knocking down regulator of G protein signaling 4 in gastric cancer cells inhibited tumor growth in vivo . A-F: Tumors from MGC-803 cells with regulator of G protein signaling 4 ( RGS4 ) knockdown and AGS cells with RGS4 overexpression and their controls ( n = 5/groups) (A and D), tumor growth curves (B and E), tumor weight of each group (C and F); G: The expression of focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial mesenchymal transition related proteins in MGC-803 cells and AGS cells in vivo were validated by western blot. β-actin was used as a loading control; H: The expression of RGS4 , Ki67 and p-AKT in these xenografts via immunohistochemistry staining; I: Schematic diagram of the mechanism of RGS4 promoting gastric cancer progression. b P < 0.01, c P < 0.001, d P < 0.0001. FAK: Focal adhesion kinase; PI3K: Phosphatidyl-inositol-3-kinase; AKT: Protein kinase B; RGS4: Regulator of G protein signaling 4; PIP: Prolactin-induced protein; mTOR: Mechanistic target of rapamycin.

Article Snippet: Next, primary antibodies [ RGS4 , ab308160, 1:1500, Abcam; focal adhesion kinase (FAK), ab40794, 1:2000, Abcam; p-FAK, ab81298, 1:1500, Abcam; phosphatidyl-inositol-3-kinase (PI3K), ab302958, 1:1500, Abcam; p-PI3K, ab182651, 1:200, Abcam; protein kinase B (AKT), ab8805, 1:500, Abcam; p-AKT, ab38449, 1:1500, Abcam; E-cadherin, ab40772, 1:1500, Abcam; N-cadherin, ab76011, 1:5000, Abcam; vimentin, ab20346, 1:1500, Abcam; β-actin, ab8227, 1:2000, Abcam] were used to nurture membranes.

Techniques: In Vivo, Knockdown, Over Expression, Expressing, Western Blot, Control, Immunohistochemistry, Staining