ptarget sequencing primer  (Promega)

 
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    Name:
    pTargeT Sequencing Primer
    Description:
    Designed for sequencing inserts cloned into the pTargeT Mammalian Expression Vector
    Catalog Number:
    q4461
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Cloning DNA Markers Molecular Biology Enzymes and Reagents
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    Structured Review

    Promega ptarget sequencing primer
    Schematic representation of the 75-bp FGG pseudoexon activated by the IVS6-320A > T mutation. (top) The fibrinogen cluster; boxes and lines represent exons and intronic/intergenic regions, respectively (only exons are drawn to scale); the two parallel slanted lines indicate breaks in the scale. (middle) The FGG minigene (M) cloned in <t>pTargeT</t> vector; the star marks the IVS6-320A > T mutation. (bottom) The complete 75-bp-long pseudoexon sequence and flaking splice sites; nucleotides belonging to the pseudoexon are in capital letters; the strength of pseudoexon splice sites, calculated by using the NNSPLICE 0.9 ( http://www.fruitfly.org/seq_tools/splice.html ) and the Netgene2 ( http://www.cbs.dtu.dk/services/NetGene2/ ) software is reported below the corresponding sequence; G-stretches are shaded in gray.
    Designed for sequencing inserts cloned into the pTargeT Mammalian Expression Vector
    https://www.bioz.com/result/ptarget sequencing primer/product/Promega
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptarget sequencing primer - by Bioz Stars, 2020-07
    93/100 stars

    Related Products / Commonly Used Together

    hepg2

    Images

    1) Product Images from "Dual Role of G-runs and hnRNP F in the Regulation of a Mutation-Activated Pseudoexon in the Fibrinogen Gamma-Chain Transcript"

    Article Title: Dual Role of G-runs and hnRNP F in the Regulation of a Mutation-Activated Pseudoexon in the Fibrinogen Gamma-Chain Transcript

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0059333

    Schematic representation of the 75-bp FGG pseudoexon activated by the IVS6-320A > T mutation. (top) The fibrinogen cluster; boxes and lines represent exons and intronic/intergenic regions, respectively (only exons are drawn to scale); the two parallel slanted lines indicate breaks in the scale. (middle) The FGG minigene (M) cloned in pTargeT vector; the star marks the IVS6-320A > T mutation. (bottom) The complete 75-bp-long pseudoexon sequence and flaking splice sites; nucleotides belonging to the pseudoexon are in capital letters; the strength of pseudoexon splice sites, calculated by using the NNSPLICE 0.9 ( http://www.fruitfly.org/seq_tools/splice.html ) and the Netgene2 ( http://www.cbs.dtu.dk/services/NetGene2/ ) software is reported below the corresponding sequence; G-stretches are shaded in gray.
    Figure Legend Snippet: Schematic representation of the 75-bp FGG pseudoexon activated by the IVS6-320A > T mutation. (top) The fibrinogen cluster; boxes and lines represent exons and intronic/intergenic regions, respectively (only exons are drawn to scale); the two parallel slanted lines indicate breaks in the scale. (middle) The FGG minigene (M) cloned in pTargeT vector; the star marks the IVS6-320A > T mutation. (bottom) The complete 75-bp-long pseudoexon sequence and flaking splice sites; nucleotides belonging to the pseudoexon are in capital letters; the strength of pseudoexon splice sites, calculated by using the NNSPLICE 0.9 ( http://www.fruitfly.org/seq_tools/splice.html ) and the Netgene2 ( http://www.cbs.dtu.dk/services/NetGene2/ ) software is reported below the corresponding sequence; G-stretches are shaded in gray.

    Techniques Used: Mutagenesis, Plasmid Preparation, Sequencing, Software

    Related Articles

    Sequencing:

    Article Title: Dual Role of G-runs and hnRNP F in the Regulation of a Mutation-Activated Pseudoexon in the Fibrinogen Gamma-Chain Transcript
    Article Snippet: .. For experiments in HepG2, the reverse primer was substituted with the commercial pTargeT sequencing primer (Promega) to discriminate transcripts produced by the transfected construct from the endogenous FGG mRNA. .. PCRs were carried out under standard conditions using the FastStart Taq DNA Polymerase (Roche) on a Mastercycler EPgradient (Eppendorf AG, Hamburg, Germany).

    Produced:

    Article Title: Dual Role of G-runs and hnRNP F in the Regulation of a Mutation-Activated Pseudoexon in the Fibrinogen Gamma-Chain Transcript
    Article Snippet: .. For experiments in HepG2, the reverse primer was substituted with the commercial pTargeT sequencing primer (Promega) to discriminate transcripts produced by the transfected construct from the endogenous FGG mRNA. .. PCRs were carried out under standard conditions using the FastStart Taq DNA Polymerase (Roche) on a Mastercycler EPgradient (Eppendorf AG, Hamburg, Germany).

    Transfection:

    Article Title: Dual Role of G-runs and hnRNP F in the Regulation of a Mutation-Activated Pseudoexon in the Fibrinogen Gamma-Chain Transcript
    Article Snippet: .. For experiments in HepG2, the reverse primer was substituted with the commercial pTargeT sequencing primer (Promega) to discriminate transcripts produced by the transfected construct from the endogenous FGG mRNA. .. PCRs were carried out under standard conditions using the FastStart Taq DNA Polymerase (Roche) on a Mastercycler EPgradient (Eppendorf AG, Hamburg, Germany).

    Construct:

    Article Title: Dual Role of G-runs and hnRNP F in the Regulation of a Mutation-Activated Pseudoexon in the Fibrinogen Gamma-Chain Transcript
    Article Snippet: .. For experiments in HepG2, the reverse primer was substituted with the commercial pTargeT sequencing primer (Promega) to discriminate transcripts produced by the transfected construct from the endogenous FGG mRNA. .. PCRs were carried out under standard conditions using the FastStart Taq DNA Polymerase (Roche) on a Mastercycler EPgradient (Eppendorf AG, Hamburg, Germany).

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  • Bioz Stars
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  • 93
    Promega ptarget sequencing primer
    Schematic representation of the 75-bp FGG pseudoexon activated by the IVS6-320A > T mutation. (top) The fibrinogen cluster; boxes and lines represent exons and intronic/intergenic regions, respectively (only exons are drawn to scale); the two parallel slanted lines indicate breaks in the scale. (middle) The FGG minigene (M) cloned in <t>pTargeT</t> vector; the star marks the IVS6-320A > T mutation. (bottom) The complete 75-bp-long pseudoexon sequence and flaking splice sites; nucleotides belonging to the pseudoexon are in capital letters; the strength of pseudoexon splice sites, calculated by using the NNSPLICE 0.9 ( http://www.fruitfly.org/seq_tools/splice.html ) and the Netgene2 ( http://www.cbs.dtu.dk/services/NetGene2/ ) software is reported below the corresponding sequence; G-stretches are shaded in gray.
    Ptarget Sequencing Primer, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptarget sequencing primer/product/Promega
    Average 93 stars, based on 77 article reviews
    Price from $9.99 to $1999.99
    ptarget sequencing primer - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Schematic representation of the 75-bp FGG pseudoexon activated by the IVS6-320A > T mutation. (top) The fibrinogen cluster; boxes and lines represent exons and intronic/intergenic regions, respectively (only exons are drawn to scale); the two parallel slanted lines indicate breaks in the scale. (middle) The FGG minigene (M) cloned in pTargeT vector; the star marks the IVS6-320A > T mutation. (bottom) The complete 75-bp-long pseudoexon sequence and flaking splice sites; nucleotides belonging to the pseudoexon are in capital letters; the strength of pseudoexon splice sites, calculated by using the NNSPLICE 0.9 ( http://www.fruitfly.org/seq_tools/splice.html ) and the Netgene2 ( http://www.cbs.dtu.dk/services/NetGene2/ ) software is reported below the corresponding sequence; G-stretches are shaded in gray.

    Journal: PLoS ONE

    Article Title: Dual Role of G-runs and hnRNP F in the Regulation of a Mutation-Activated Pseudoexon in the Fibrinogen Gamma-Chain Transcript

    doi: 10.1371/journal.pone.0059333

    Figure Lengend Snippet: Schematic representation of the 75-bp FGG pseudoexon activated by the IVS6-320A > T mutation. (top) The fibrinogen cluster; boxes and lines represent exons and intronic/intergenic regions, respectively (only exons are drawn to scale); the two parallel slanted lines indicate breaks in the scale. (middle) The FGG minigene (M) cloned in pTargeT vector; the star marks the IVS6-320A > T mutation. (bottom) The complete 75-bp-long pseudoexon sequence and flaking splice sites; nucleotides belonging to the pseudoexon are in capital letters; the strength of pseudoexon splice sites, calculated by using the NNSPLICE 0.9 ( http://www.fruitfly.org/seq_tools/splice.html ) and the Netgene2 ( http://www.cbs.dtu.dk/services/NetGene2/ ) software is reported below the corresponding sequence; G-stretches are shaded in gray.

    Article Snippet: For experiments in HepG2, the reverse primer was substituted with the commercial pTargeT sequencing primer (Promega) to discriminate transcripts produced by the transfected construct from the endogenous FGG mRNA.

    Techniques: Mutagenesis, Plasmid Preparation, Sequencing, Software