psti  (Thermo Fisher)


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    Name:
    PstI 10 U µL
    Description:
    5 C T G C A ↓G 3 3 G ↑A C G T C 5 Thermo Scientific PstI restriction enzyme recognizes CTGCA G sites and cuts best at 37°C in O buffer Isoschizomers BspMAI See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0611
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher psti
    Circularization of <t>pBR322</t> (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by <t>PstI.</t> Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.
    5 C T G C A ↓G 3 3 G ↑A C G T C 5 Thermo Scientific PstI restriction enzyme recognizes CTGCA G sites and cuts best at 37°C in O buffer Isoschizomers BspMAI See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/psti/product/Thermo Fisher
    Average 98 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    psti - by Bioz Stars, 2020-09
    98/100 stars

    Images

    1) Product Images from "In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium"

    Article Title: In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0177751

    Circularization of pBR322 (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by PstI. Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.
    Figure Legend Snippet: Circularization of pBR322 (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by PstI. Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.

    Techniques Used: Electron Microscopy, Plasmid Preparation

    2) Product Images from "In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium"

    Article Title: In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0177751

    Circularization of pBR322 (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by PstI. Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.
    Figure Legend Snippet: Circularization of pBR322 (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by PstI. Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.

    Techniques Used: Electron Microscopy, Plasmid Preparation

    Related Articles

    Clone Assay:

    Article Title: An enhanced U6 promoter for synthesis of short hairpin RNA
    Article Snippet: .. Briefly, mutant human SOD1G93A cDNA was PCR cloned between the PmlI and PstI sites of pCMV/myc/mito/GFP (Invitrogen). .. This cloning step deleted the mitochondrial targeting sequence.

    Agarose Gel Electrophoresis:

    Article Title: DEV induce autophagy via the endoplasmic reticulum stress related unfolded protein response
    Article Snippet: .. The PCR products were further digested with the restriction enzyme PstI (Thermo Fisher Scientific, Waltham, MA, USA) and then separated on a 1% agarose gel. .. As an internal control, DNA complementary to the GAPDH messenger RNA (mRNA) was also amplified using the forward primer 5′-AGATGCTGGTGCTGAATACG-3′ and the reverse primer 5′ -CGGAGATGATGACACGCTTA-3′ .

    Ligation:

    Article Title: Genetic structure and seed-mediated dispersal rates of an endangered shrub in a fragmented landscape: a case study for Juniperus communis in northwestern Europe
    Article Snippet: .. Restriction and ligation were performed in a single step, e.g. 200 ng of genomic DNA was restriction digested using the enzyme combination PstI (Fermentas) /MseI (Fermentas) and ligated to the PstI and MseI adaptors. .. Primer combinations used for the generation of fingerprints were PstI -ACT + MseI -ACA, PstI- ACT + MseI -ACC, PstI -AGT + MseI -ACC and PstI -AGT + MseI -ACA.

    Mutagenesis:

    Article Title: An enhanced U6 promoter for synthesis of short hairpin RNA
    Article Snippet: .. Briefly, mutant human SOD1G93A cDNA was PCR cloned between the PmlI and PstI sites of pCMV/myc/mito/GFP (Invitrogen). .. This cloning step deleted the mitochondrial targeting sequence.

    Electrophoretic Mobility Shift Assay:

    Article Title: In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium
    Article Snippet: .. Electrophoretic Mobility Shift Assay (EMSA) 200 ng of supercoiled pBR322 or pBR322 linearized by PstI (Thermo-Scientific) (4361 bp), as well as 200 ng of RFI (5386 bp) or single-stranded DNA of phiX174 virion were incubated in 20 μl of buffer A (40 mM Tris-HCl pH 7.8, 5 mM MgCl2 , 1.5 mM DTT, 50 mM NaCl, 12% glycerol) with increasing concentrations of DdrC (0.86 μM, 1.7 μM, 3.5 μM, 7 μM and 8.6 μM). .. After 15 min incubation at 4°C, 4 μl of 6X DNA Loading Dye (Fermentas) were added to the mixture before electrophoresis through 1.2% agarose gels.

    Purification:

    Article Title: In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium
    Article Snippet: .. Effect of DdrC on T4 DNA ligase activity 200 ng of purified pBR322 DNA molecules digested by PstI (Thermo-Scientific) were preincubated at 4°C for 15 min in the absence or the presence of increasing concentrations of DdrC protein (0.1 μM, 0.2 μM, 1 μM, 2 μM and 4 μM) prior to addition of T4 DNA ligase (Thermo-Scientific). .. The reactions were stopped after 15 min at 30°C by addition of a mix of Proteinase K (1 mg/ml) and SDS (0.5%) (10 min at 37°C) and samples were loaded on a 1% agarose gel.

    Incubation:

    Article Title: In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium
    Article Snippet: .. Electrophoretic Mobility Shift Assay (EMSA) 200 ng of supercoiled pBR322 or pBR322 linearized by PstI (Thermo-Scientific) (4361 bp), as well as 200 ng of RFI (5386 bp) or single-stranded DNA of phiX174 virion were incubated in 20 μl of buffer A (40 mM Tris-HCl pH 7.8, 5 mM MgCl2 , 1.5 mM DTT, 50 mM NaCl, 12% glycerol) with increasing concentrations of DdrC (0.86 μM, 1.7 μM, 3.5 μM, 7 μM and 8.6 μM). .. After 15 min incubation at 4°C, 4 μl of 6X DNA Loading Dye (Fermentas) were added to the mixture before electrophoresis through 1.2% agarose gels.

    Activity Assay:

    Article Title: In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium
    Article Snippet: .. Effect of DdrC on T4 DNA ligase activity 200 ng of purified pBR322 DNA molecules digested by PstI (Thermo-Scientific) were preincubated at 4°C for 15 min in the absence or the presence of increasing concentrations of DdrC protein (0.1 μM, 0.2 μM, 1 μM, 2 μM and 4 μM) prior to addition of T4 DNA ligase (Thermo-Scientific). .. The reactions were stopped after 15 min at 30°C by addition of a mix of Proteinase K (1 mg/ml) and SDS (0.5%) (10 min at 37°C) and samples were loaded on a 1% agarose gel.

    Polymerase Chain Reaction:

    Article Title: DEV induce autophagy via the endoplasmic reticulum stress related unfolded protein response
    Article Snippet: .. The PCR products were further digested with the restriction enzyme PstI (Thermo Fisher Scientific, Waltham, MA, USA) and then separated on a 1% agarose gel. .. As an internal control, DNA complementary to the GAPDH messenger RNA (mRNA) was also amplified using the forward primer 5′-AGATGCTGGTGCTGAATACG-3′ and the reverse primer 5′ -CGGAGATGATGACACGCTTA-3′ .

    Article Title: The C-Terminal nsP1a Protein of Human Astrovirus Is a Phosphoprotein That Interacts with the Viral Polymerase ▿
    Article Snippet: .. PCR-amplified fragments flanked by NotI and PstI restriction enzyme sites were ligated into NotI/PstI-digested pFastBAC (Invitrogen) baculovirus transfer vector and transformed into DH10Bac competent cells, which contain bacmid DNA. .. Colonies containing recombinant bacmids were identified by disruption of the lacZ α gene.

    Article Title: An enhanced U6 promoter for synthesis of short hairpin RNA
    Article Snippet: .. Briefly, mutant human SOD1G93A cDNA was PCR cloned between the PmlI and PstI sites of pCMV/myc/mito/GFP (Invitrogen). .. This cloning step deleted the mitochondrial targeting sequence.

    Transformation Assay:

    Article Title: The C-Terminal nsP1a Protein of Human Astrovirus Is a Phosphoprotein That Interacts with the Viral Polymerase ▿
    Article Snippet: .. PCR-amplified fragments flanked by NotI and PstI restriction enzyme sites were ligated into NotI/PstI-digested pFastBAC (Invitrogen) baculovirus transfer vector and transformed into DH10Bac competent cells, which contain bacmid DNA. .. Colonies containing recombinant bacmids were identified by disruption of the lacZ α gene.

    Plasmid Preparation:

    Article Title: A New Bacterial Disease on Mandevilla sanderi, Caused by Pseudomonas savastanoi: Lessons Learned for Bacterial Diversity Studies
    Article Snippet: .. Plasmid DNA left undigested or digested with Bst1107I and PstI enzymes (Fermentas) was analyzed in 0.8% or 1% agarose gels, respectively. .. All strains contained plasmids and displayed multiple plasmid restriction patterns.

    Article Title: The C-Terminal nsP1a Protein of Human Astrovirus Is a Phosphoprotein That Interacts with the Viral Polymerase ▿
    Article Snippet: .. PCR-amplified fragments flanked by NotI and PstI restriction enzyme sites were ligated into NotI/PstI-digested pFastBAC (Invitrogen) baculovirus transfer vector and transformed into DH10Bac competent cells, which contain bacmid DNA. .. Colonies containing recombinant bacmids were identified by disruption of the lacZ α gene.

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  • 98
    Thermo Fisher psti
    Circularization of <t>pBR322</t> (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by <t>PstI.</t> Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.
    Psti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti/product/Thermo Fisher
    Average 98 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    psti - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    Circularization of pBR322 (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by PstI. Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.

    Journal: PLoS ONE

    Article Title: In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium

    doi: 10.1371/journal.pone.0177751

    Figure Lengend Snippet: Circularization of pBR322 (cohesive ends) and pUC19 (blunt ends) plasmids mediated by DdrC visualized by electron microscopy. Panel a: Control pBR322 DNA linearized by PstI. Panels b-e: pBR322 circularization mediated by DdrC. Panel f. Control pUC19 DNA linearized by Ssp11. Panels g-j: pUC19 circularization mediated by DdrC. 1 μM DdrC was mixed with 2 nM molecules of linear pBR322 or pUC19 plasmid, containing cohesive or blunt ends, respectively. The shapes are similar at 0.5 μM, 1 μM or 2 μM of DdrC. Magnification = 85,000. Some loci of plasmid circularization are indicated by arrows.

    Article Snippet: Electrophoretic Mobility Shift Assay (EMSA) 200 ng of supercoiled pBR322 or pBR322 linearized by PstI (Thermo-Scientific) (4361 bp), as well as 200 ng of RFI (5386 bp) or single-stranded DNA of phiX174 virion were incubated in 20 μl of buffer A (40 mM Tris-HCl pH 7.8, 5 mM MgCl2 , 1.5 mM DTT, 50 mM NaCl, 12% glycerol) with increasing concentrations of DdrC (0.86 μM, 1.7 μM, 3.5 μM, 7 μM and 8.6 μM).

    Techniques: Electron Microscopy, Plasmid Preparation

    PERK and IRE1 pathways was activated under ER stress and was involved in DEV-induced autophagy. (A) DEF cells were infected with DEV at a MOI of 1 or treated with 300 nM Tg for 24 hours, and whole-cell protein extracts were collected at 36, 48, and 60 hours post-infection and then subjected to immunoblotting analysis of GRP78, p-PERK, p-eIF2α, LC3, ATF4, p-IRE1 and β-actin using the indicated antibodies. Tg is an inducer of ER stress. (B) Ratios of GRP78 to β-actin, p-PERK to PERK, p-eIF2α to eIF2α, ATF4 to actin, and p-IRE1 to IRE1, and LC3-II to LC3-I. (C) Activation of the IRE1-XBP1 signaling pathway. Total RNA was isolated from DEF cells, and RT-PCR was performed using XBP1-specific primers. PCR products were digested with PstI. Spliced XBP1 (s) products, 549 bp; Unspliced XBP1 (u) products, 276 bp; GAPDH was used as the loading control. (D) Inactivation of the ATF6 signaling pathway. DEF cells were infected with DEV at a MOI of 1 or treated with 300 nM Tg for 24 hours, and whole-cell protein extracts were collected at 36, 48, and 60 hours post-infection and then subjected to immunoblotting analysis of ATF6 and β-actin using the indicated antibodies.

    Journal: PLoS ONE

    Article Title: DEV induce autophagy via the endoplasmic reticulum stress related unfolded protein response

    doi: 10.1371/journal.pone.0189704

    Figure Lengend Snippet: PERK and IRE1 pathways was activated under ER stress and was involved in DEV-induced autophagy. (A) DEF cells were infected with DEV at a MOI of 1 or treated with 300 nM Tg for 24 hours, and whole-cell protein extracts were collected at 36, 48, and 60 hours post-infection and then subjected to immunoblotting analysis of GRP78, p-PERK, p-eIF2α, LC3, ATF4, p-IRE1 and β-actin using the indicated antibodies. Tg is an inducer of ER stress. (B) Ratios of GRP78 to β-actin, p-PERK to PERK, p-eIF2α to eIF2α, ATF4 to actin, and p-IRE1 to IRE1, and LC3-II to LC3-I. (C) Activation of the IRE1-XBP1 signaling pathway. Total RNA was isolated from DEF cells, and RT-PCR was performed using XBP1-specific primers. PCR products were digested with PstI. Spliced XBP1 (s) products, 549 bp; Unspliced XBP1 (u) products, 276 bp; GAPDH was used as the loading control. (D) Inactivation of the ATF6 signaling pathway. DEF cells were infected with DEV at a MOI of 1 or treated with 300 nM Tg for 24 hours, and whole-cell protein extracts were collected at 36, 48, and 60 hours post-infection and then subjected to immunoblotting analysis of ATF6 and β-actin using the indicated antibodies.

    Article Snippet: The PCR products were further digested with the restriction enzyme PstI (Thermo Fisher Scientific, Waltham, MA, USA) and then separated on a 1% agarose gel.

    Techniques: Infection, Activation Assay, Isolation, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction

    Agarose gel electrophoresis of the VHH PCR fragments and the pHEN4 phagemid digested with NotI and PstI restriction enzymes before ligation and transformation of TG1 bacteria.

    Journal: Iranian Journal of Biotechnology

    Article Title: Construction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary

    doi: 10.30498/IJB.2020.127753.2247

    Figure Lengend Snippet: Agarose gel electrophoresis of the VHH PCR fragments and the pHEN4 phagemid digested with NotI and PstI restriction enzymes before ligation and transformation of TG1 bacteria.

    Article Snippet: The PCR fragments were ligated into the phagemid vector pHEN4 ( ) after cutting with the restriction enzymes PstI and NotI.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Ligation, Transformation Assay