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Promega psti enzyme
Psti Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psti enzyme/product/Promega
Average 88 stars, based on 3 article reviews
Price from $9.99 to $1999.99
psti enzyme - by Bioz Stars, 2020-09
88/100 stars

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Polymerase Chain Reaction:

Article Title: XPD gene polymorphism and host characteristics in the association with cutaneous malignant melanoma risk
Article Snippet: .. The PCR product was digested with 10–15 U of PstI enzyme (Promega, Madison, WI, USA) in a 25-μ l-reaction mixture for 2 h at 37°C, as suggested by the manufacturer, and separated on a 2% agarose gel. ..

Nucleic Acid Electrophoresis:

Article Title: Association of Transferable Quinolone Resistance Determinant qnrB19 with Extended-Spectrum β-Lactamases in Salmonella Give and Salmonella Heidelberg in Venezuela
Article Snippet: .. These plasmids were analyzed by restriction mapping using the HindIII and PstI enzyme (Promega, Madison, WI, USA) to observe the restriction patterns, and the resulting fragment sizes were determined by gel electrophoresis. .. Transformation Experiment ESBL and Qnr-harboring plasmids were transferred into E. coli HB101 competent cells by electroporation (Eppendorf model 2510, Germany) using approximately 0.5 μ g of the plasmid DNA preparation under conditions recommended by manufacturer.

Alkaline Lysis:

Article Title: Evolution of an Incompatibility Group IncA/C Plasmid Harboring blaCMY-16 and qnrA6 Genes and Its Transfer through Three Clones of Providencia stuartii during a Two-Year Outbreak in a Tunisian Burn Unit
Article Snippet: .. Then, plasmid DNA from transconjugants was extracted using an alkaline lysis method ( ) and was digested with the PstI enzyme (Promega SA). .. Furthermore, the unrestricted plasmid profiles were visualized after DNA linearization with S1 nuclease, followed by PFGE (S1-PFGE), as reported elsewhere ( ).

Agarose Gel Electrophoresis:

Article Title: XPD gene polymorphism and host characteristics in the association with cutaneous malignant melanoma risk
Article Snippet: .. The PCR product was digested with 10–15 U of PstI enzyme (Promega, Madison, WI, USA) in a 25-μ l-reaction mixture for 2 h at 37°C, as suggested by the manufacturer, and separated on a 2% agarose gel. ..

Plasmid Preparation:

Article Title: Evolution of an Incompatibility Group IncA/C Plasmid Harboring blaCMY-16 and qnrA6 Genes and Its Transfer through Three Clones of Providencia stuartii during a Two-Year Outbreak in a Tunisian Burn Unit
Article Snippet: .. Then, plasmid DNA from transconjugants was extracted using an alkaline lysis method ( ) and was digested with the PstI enzyme (Promega SA). .. Furthermore, the unrestricted plasmid profiles were visualized after DNA linearization with S1 nuclease, followed by PFGE (S1-PFGE), as reported elsewhere ( ).

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    Promega restriction enzyme psti
    (A) XBP1 transcript splicing in NIH 3T3 cells infected (MOI, 30) with SFV4 or VRPs. RNA from infected cells was reverse transcribed into cDNA, and XBP1 amplicons were generated by <t>PCR.</t> These were digested with <t>PstI,</t> which only cleaves amplicons derived
    Restriction Enzyme Psti, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme psti/product/Promega
    Average 89 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme psti - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

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    (A) XBP1 transcript splicing in NIH 3T3 cells infected (MOI, 30) with SFV4 or VRPs. RNA from infected cells was reverse transcribed into cDNA, and XBP1 amplicons were generated by PCR. These were digested with PstI, which only cleaves amplicons derived

    Journal: Journal of Virology

    Article Title: Semliki Forest Virus-Induced Endoplasmic Reticulum Stress Accelerates Apoptotic Death of Mammalian Cells ▿Semliki Forest Virus-Induced Endoplasmic Reticulum Stress Accelerates Apoptotic Death of Mammalian Cells ▿ †

    doi: 10.1128/JVI.02310-09

    Figure Lengend Snippet: (A) XBP1 transcript splicing in NIH 3T3 cells infected (MOI, 30) with SFV4 or VRPs. RNA from infected cells was reverse transcribed into cDNA, and XBP1 amplicons were generated by PCR. These were digested with PstI, which only cleaves amplicons derived

    Article Snippet: The PCR product then was treated with the restriction enzyme PstI (Promega) and run on an agarose gel.

    Techniques: Infection, Generated, Polymerase Chain Reaction, Derivative Assay

    Human and murine melanoma cell lysates induce expression of XBP1s and additional members of the UPR in murine BMDCs. (A) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml cell lysate from human melanoma cell lines (MEL), 100 ng/ml lipopolysaccharide (LPS), 50 μg/ml house dust mite extract (HDM), or 1 μg/ml tunicamycin (TM) for 8 h. Expression of Xbp1s was determined by a RT-PCR protocol for Xbp1s and Xbp1u that includes a digestion step with the restriction enzyme PstI. The Pst I digestion site in the intron of Xbp1u mRNA allows the distinction between Xbp1s and two fragments of Xbp1u mRNA. A representative scheme is illustrated. Data is representative of three independent experiments. (B) FL-DCs were stimulated as in (A) and expression of Xbp1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs depicts three independent experiments. (C) FL-DCs generated from ERAI mice were left untreated (NT) or stimulated with 25, 50, 100, and 200 μg/ml of MEL for 16 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (D) ERAI FL-DCs were NT or stimulated with 100 μg/ml MEL for 24 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ), cDC2 FL-DC (SIPRα + ), and pDC FL-DC (B220 + ). (E) ERAI FL-DCs were NT or stimulated with 100 ug/ml MEL, 100 ug/ml human leukocyte cell lysate or 100 ug/ml B16F10 murine melanoma cell lysates (B16 lysate) for 24 h to evaluate VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (F) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s was measured by qPCR. Data in graphs depicts three independent experiments. (G) GMCSF BMDCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs show two independent experiments. For (C – E) , each symbol in the graph represents data derived from one independent experiment. For all error bars represent mean ± SEM. * p

    Journal: Frontiers in Immunology

    Article Title: IRE1α Activation in Bone Marrow-Derived Dendritic Cells Modulates Innate Recognition of Melanoma Cells and Favors CD8+ T Cell Priming

    doi: 10.3389/fimmu.2018.03050

    Figure Lengend Snippet: Human and murine melanoma cell lysates induce expression of XBP1s and additional members of the UPR in murine BMDCs. (A) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml cell lysate from human melanoma cell lines (MEL), 100 ng/ml lipopolysaccharide (LPS), 50 μg/ml house dust mite extract (HDM), or 1 μg/ml tunicamycin (TM) for 8 h. Expression of Xbp1s was determined by a RT-PCR protocol for Xbp1s and Xbp1u that includes a digestion step with the restriction enzyme PstI. The Pst I digestion site in the intron of Xbp1u mRNA allows the distinction between Xbp1s and two fragments of Xbp1u mRNA. A representative scheme is illustrated. Data is representative of three independent experiments. (B) FL-DCs were stimulated as in (A) and expression of Xbp1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs depicts three independent experiments. (C) FL-DCs generated from ERAI mice were left untreated (NT) or stimulated with 25, 50, 100, and 200 μg/ml of MEL for 16 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (D) ERAI FL-DCs were NT or stimulated with 100 μg/ml MEL for 24 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ), cDC2 FL-DC (SIPRα + ), and pDC FL-DC (B220 + ). (E) ERAI FL-DCs were NT or stimulated with 100 ug/ml MEL, 100 ug/ml human leukocyte cell lysate or 100 ug/ml B16F10 murine melanoma cell lysates (B16 lysate) for 24 h to evaluate VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (F) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s was measured by qPCR. Data in graphs depicts three independent experiments. (G) GMCSF BMDCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs show two independent experiments. For (C – E) , each symbol in the graph represents data derived from one independent experiment. For all error bars represent mean ± SEM. * p

    Article Snippet: Briefly, cDNA was amplificated and PCR products were digested with the restriction enzyme PstI (Promega) for 2 h and then analyzed in a 1% agarose gel.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Generated, Mouse Assay, Derivative Assay

    Human and murine melanoma cell lysates induce expression of XBP1s and additional members of the UPR in murine BMDCs. (A) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml cell lysate from human melanoma cell lines (MEL), 100 ng/ml lipopolysaccharide (LPS), 50 μg/ml house dust mite extract (HDM), or 1 μg/ml tunicamycin (TM) for 8 h. Expression of Xbp1s was determined by a RT-PCR protocol for Xbp1s and Xbp1u that includes a digestion step with the restriction enzyme PstI. The Pst I digestion site in the intron of Xbp1u mRNA allows the distinction between Xbp1s and two fragments of Xbp1u mRNA. A representative scheme is illustrated. Data is representative of three independent experiments. (B) FL-DCs were stimulated as in (A) and expression of Xbp1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs depicts three independent experiments. (C) FL-DCs generated from ERAI mice were left untreated (NT) or stimulated with 25, 50, 100, and 200 μg/ml of MEL for 16 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (D) ERAI FL-DCs were NT or stimulated with 100 μg/ml MEL for 24 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ), cDC2 FL-DC (SIPRα + ), and pDC FL-DC (B220 + ). (E) ERAI FL-DCs were NT or stimulated with 100 ug/ml MEL, 100 ug/ml human leukocyte cell lysate or 100 ug/ml B16F10 murine melanoma cell lysates (B16 lysate) for 24 h to evaluate VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (F) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s was measured by qPCR. Data in graphs depicts three independent experiments. (G) GMCSF BMDCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs show two independent experiments. For (C – E) , each symbol in the graph represents data derived from one independent experiment. For all error bars represent mean ± SEM. * p

    Journal: Frontiers in Immunology

    Article Title: IRE1α Activation in Bone Marrow-Derived Dendritic Cells Modulates Innate Recognition of Melanoma Cells and Favors CD8+ T Cell Priming

    doi: 10.3389/fimmu.2018.03050

    Figure Lengend Snippet: Human and murine melanoma cell lysates induce expression of XBP1s and additional members of the UPR in murine BMDCs. (A) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml cell lysate from human melanoma cell lines (MEL), 100 ng/ml lipopolysaccharide (LPS), 50 μg/ml house dust mite extract (HDM), or 1 μg/ml tunicamycin (TM) for 8 h. Expression of Xbp1s was determined by a RT-PCR protocol for Xbp1s and Xbp1u that includes a digestion step with the restriction enzyme PstI. The Pst I digestion site in the intron of Xbp1u mRNA allows the distinction between Xbp1s and two fragments of Xbp1u mRNA. A representative scheme is illustrated. Data is representative of three independent experiments. (B) FL-DCs were stimulated as in (A) and expression of Xbp1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs depicts three independent experiments. (C) FL-DCs generated from ERAI mice were left untreated (NT) or stimulated with 25, 50, 100, and 200 μg/ml of MEL for 16 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (D) ERAI FL-DCs were NT or stimulated with 100 μg/ml MEL for 24 h for the quantification of VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ), cDC2 FL-DC (SIPRα + ), and pDC FL-DC (B220 + ). (E) ERAI FL-DCs were NT or stimulated with 100 ug/ml MEL, 100 ug/ml human leukocyte cell lysate or 100 ug/ml B16F10 murine melanoma cell lysates (B16 lysate) for 24 h to evaluate VenusFP expression. Data in graphs depicts the MFI of cDC1 FL-DC (XCR1 + ) of three independent experiments. (F) FL-DCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s was measured by qPCR. Data in graphs depicts three independent experiments. (G) GMCSF BMDCs were left untreated (NT) or stimulated with 100 μg/ml B16 lysate or 1 μg/ml TM for 8 h and expression of XBP-1s, BiP , and CHOP mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs show two independent experiments. For (C – E) , each symbol in the graph represents data derived from one independent experiment. For all error bars represent mean ± SEM. * p

    Article Snippet: Briefly, cDNA was amplificated and PCR products were digested with the restriction enzyme PstI (Promega) for 2 h and then analyzed in a 1% agarose gel.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Generated, Mouse Assay, Derivative Assay