pspxi  (New England Biolabs)


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    New England Biolabs pspxi
    Validation of cloned murine GM-CSF. (A) Image of <t>PspXI</t> and NotI digested <t>PCR</t> product of murine GM-CSF. (B) Detailed 266 vector map of mGM-CSF, restriction sites, promoter, antibiotic selection and mCherry sequence. (C) Alignment of Sanger sequencing results of mGM-CSF and 266 Vector map strategy. (D) Quality of sanger sequencing results of murine GM-CSF.
    Pspxi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pspxi/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pspxi - by Bioz Stars, 2022-07
    94/100 stars

    Images

    1) Product Images from "Generation and utilization of a HEK-293T murine GM-CSF expressing cell line"

    Article Title: Generation and utilization of a HEK-293T murine GM-CSF expressing cell line

    Journal: bioRxiv

    doi: 10.1101/2020.06.18.160366

    Validation of cloned murine GM-CSF. (A) Image of PspXI and NotI digested PCR product of murine GM-CSF. (B) Detailed 266 vector map of mGM-CSF, restriction sites, promoter, antibiotic selection and mCherry sequence. (C) Alignment of Sanger sequencing results of mGM-CSF and 266 Vector map strategy. (D) Quality of sanger sequencing results of murine GM-CSF.
    Figure Legend Snippet: Validation of cloned murine GM-CSF. (A) Image of PspXI and NotI digested PCR product of murine GM-CSF. (B) Detailed 266 vector map of mGM-CSF, restriction sites, promoter, antibiotic selection and mCherry sequence. (C) Alignment of Sanger sequencing results of mGM-CSF and 266 Vector map strategy. (D) Quality of sanger sequencing results of murine GM-CSF.

    Techniques Used: Clone Assay, Polymerase Chain Reaction, Plasmid Preparation, Selection, Sequencing

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  • News
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  • 94
    New England Biolabs pspxi
    Validation of cloned murine GM-CSF. (A) Image of <t>PspXI</t> and NotI digested <t>PCR</t> product of murine GM-CSF. (B) Detailed 266 vector map of mGM-CSF, restriction sites, promoter, antibiotic selection and mCherry sequence. (C) Alignment of Sanger sequencing results of mGM-CSF and 266 Vector map strategy. (D) Quality of sanger sequencing results of murine GM-CSF.
    Pspxi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pspxi/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pspxi - by Bioz Stars, 2022-07
    94/100 stars
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    Validation of cloned murine GM-CSF. (A) Image of PspXI and NotI digested PCR product of murine GM-CSF. (B) Detailed 266 vector map of mGM-CSF, restriction sites, promoter, antibiotic selection and mCherry sequence. (C) Alignment of Sanger sequencing results of mGM-CSF and 266 Vector map strategy. (D) Quality of sanger sequencing results of murine GM-CSF.

    Journal: bioRxiv

    Article Title: Generation and utilization of a HEK-293T murine GM-CSF expressing cell line

    doi: 10.1101/2020.06.18.160366

    Figure Lengend Snippet: Validation of cloned murine GM-CSF. (A) Image of PspXI and NotI digested PCR product of murine GM-CSF. (B) Detailed 266 vector map of mGM-CSF, restriction sites, promoter, antibiotic selection and mCherry sequence. (C) Alignment of Sanger sequencing results of mGM-CSF and 266 Vector map strategy. (D) Quality of sanger sequencing results of murine GM-CSF.

    Article Snippet: The PCR product was purified using the PCR Clean-up Kit (Macherey-Nagel) and was subsequently digested with PspXI (New England BioLabs) and NotI-HF (New England BioLabs) using recommended digestion conditions ( https://nebcloner.neb.com/#!/redigest) and was cloned into our custom 681 bidirectional lentiviral vector (sequence in supplemental).

    Techniques: Clone Assay, Polymerase Chain Reaction, Plasmid Preparation, Selection, Sequencing