Journal: Bioactive Materials

Article Title: Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation

doi: 10.1016/j.bioactmat.2025.12.026

Figure Lengend Snippet: Bacterial viability of P. aeruginosa (a) and S. aureus (b). i) Quantitative analysis of the % of dead/total bacteria at 6 h incubation on the different CaP discs obtained by hydrolysis of α-TCP in sodium fluoride solutions (0 mM [0F], 0.5 mM [05F], and 10 mM [10F]) either in biomimetic (BM) or hydrothermal (AC) conditions and merged CLSM images of live/dead staining for representative samples. As controls, Flat and re-pressed fluoride-doped CaP nanostructures (CXF_AC or CXF_BM) were used. Red bacteria correspond to bacteria with damaged membrane, green bacteria correspond to viable bacteria. ii) BacTiterGlo measurements normalized with the area of bacteria adhered at 6 h of incubation on the CaP substrates. The red dotted line indicates the normalized metabolic activity of a Ti control. iii) SEM images of P. aeruginosa and S. aureus cultured onto different CaP discs. n = 3; ns, ∗, ∗∗, ∗∗∗ and ∗∗∗∗ indicate significance at p > 0.05, p ≤ 0.05, p ≤ 0.01, p ≤ 0.001 and p ≤ 0.0001, respectively.

Article Snippet: SaOS-2 cell line (HTB-85), RAW 264.7 cell line (TIB-71), Pseudomonas aeruginosa (ATCC-27853) and Staphylococcus aureus (ATCC-25923) were purchased from American Type Culture Collection (VA, USA).

Techniques: Bacteria, Incubation, Staining, Membrane, Activity Assay, Control, Cell Culture

Journal: Bioactive Materials

Article Title: Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation

doi: 10.1016/j.bioactmat.2025.12.026

Figure Lengend Snippet: Co-culture of P. aeruginosa and SaOS-2 cells on the nanostructured CaP discs, as well as Flat and Ti controls. a) Merged CLSM images of: i) a pre-implantation infection model, where the samples were first incubated for 6 h with P. aeruginosa and subsequently SaOS-2 cells were cultured for 24 h; or ii) post-implantation infection model, where SaOS-2 cells were first cultured for 24 h on the substrates, which were subsequently incubated for 6 h with P. aeruginosa . Actin filaments were stained with Alexa Fluor™ 546 phalloidin (orange fluorescence signal) and the nuclei with DAPI (blue fluorescence signal). (b) Orthogonal CLSM images showing simultaneous co-visualization of cells and bacteria stained with Alexa Fluor™ 546 phalloidin (orange fluorescence signal), the nuclei with DAPI (blue fluorescence signal) and SYTO-9 (green fluorescence signal). (c) Dead percentage of P. aeruginosa onto the different CaP substrates and the controls, for both the pre-implantation infection i) and the post-implantation ii) infection models. n = 3; ns and ∗ indicate significance at p > 0.05 and p ≤ 0.05, respectively.

Article Snippet: SaOS-2 cell line (HTB-85), RAW 264.7 cell line (TIB-71), Pseudomonas aeruginosa (ATCC-27853) and Staphylococcus aureus (ATCC-25923) were purchased from American Type Culture Collection (VA, USA).

Techniques: Co-Culture Assay, Infection, Incubation, Cell Culture, Staining, Fluorescence, Bacteria

Journal: Bioactive Materials

Article Title: Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation

doi: 10.1016/j.bioactmat.2025.12.026

Figure Lengend Snippet: Bacterial viability of P. aeruginosa (a) and S. aureus (b). i) Quantitative analysis of the % of dead/total bacteria at 6 h incubation on the different CaP discs obtained by hydrolysis of α-TCP in sodium fluoride solutions (0 mM [0F], 0.5 mM [05F], and 10 mM [10F]) either in biomimetic (BM) or hydrothermal (AC) conditions and merged CLSM images of live/dead staining for representative samples. As controls, Flat and re-pressed fluoride-doped CaP nanostructures (CXF_AC or CXF_BM) were used. Red bacteria correspond to bacteria with damaged membrane, green bacteria correspond to viable bacteria. ii) BacTiterGlo measurements normalized with the area of bacteria adhered at 6 h of incubation on the CaP substrates. The red dotted line indicates the normalized metabolic activity of a Ti control. iii) SEM images of P. aeruginosa and S. aureus cultured onto different CaP discs. n = 3; ns, ∗, ∗∗, ∗∗∗ and ∗∗∗∗ indicate significance at p > 0.05, p ≤ 0.05, p ≤ 0.01, p ≤ 0.001 and p ≤ 0.0001, respectively.

Article Snippet: Bacterial assays were conducted using two strains: Gram-negative P. aeruginosa (ATCC 27853) and Gram-positive S. aureus (ATCC 25923).

Techniques: Bacteria, Incubation, Staining, Membrane, Activity Assay, Control, Cell Culture

Journal: Bioactive Materials

Article Title: Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation

doi: 10.1016/j.bioactmat.2025.12.026

Figure Lengend Snippet: Co-culture of P. aeruginosa and SaOS-2 cells on the nanostructured CaP discs, as well as Flat and Ti controls. a) Merged CLSM images of: i) a pre-implantation infection model, where the samples were first incubated for 6 h with P. aeruginosa and subsequently SaOS-2 cells were cultured for 24 h; or ii) post-implantation infection model, where SaOS-2 cells were first cultured for 24 h on the substrates, which were subsequently incubated for 6 h with P. aeruginosa . Actin filaments were stained with Alexa Fluor™ 546 phalloidin (orange fluorescence signal) and the nuclei with DAPI (blue fluorescence signal). (b) Orthogonal CLSM images showing simultaneous co-visualization of cells and bacteria stained with Alexa Fluor™ 546 phalloidin (orange fluorescence signal), the nuclei with DAPI (blue fluorescence signal) and SYTO-9 (green fluorescence signal). (c) Dead percentage of P. aeruginosa onto the different CaP substrates and the controls, for both the pre-implantation infection i) and the post-implantation ii) infection models. n = 3; ns and ∗ indicate significance at p > 0.05 and p ≤ 0.05, respectively.

Article Snippet: Bacterial assays were conducted using two strains: Gram-negative P. aeruginosa (ATCC 27853) and Gram-positive S. aureus (ATCC 25923).

Techniques: Co-Culture Assay, Infection, Incubation, Cell Culture, Staining, Fluorescence, Bacteria

Journal: iScience

Article Title: Structure and function of persulfide dioxygenase from Pseudomonas aeruginosa : Implications on H 2 S homeostasis and interplay with nitric oxide

doi: 10.1016/j.isci.2025.114586

Figure Lengend Snippet: Role of the gene pdo in H 2 S catabolism in P a H 2 S levels detected with PAO1, its isogenic Δ pdo mutant (blue bars), and the same strains carrying either the pUCP18 empty vector or the pUCP18-derived plasmid pUCP- pdo for constitutive pdo expression (orange bars). Data are reported as the percentage relative to PAO1. The mean and standard deviations were obtained from five independent experiments. p values are indicated.

Article Snippet: Pseudomonas aeruginosa PAO1 , ATCC , ATCC15692.

Techniques: Mutagenesis, Plasmid Preparation, Derivative Assay, Expressing