asic1a specific inhibitor psalmotoxin 1 pctx1  (ApexBio)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    ApexBio asic1a specific inhibitor psalmotoxin 1 pctx1
    Exposure to an acidic medium made the expression of <t>ASIC1a</t> in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Asic1a Specific Inhibitor Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1a specific inhibitor psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic1a specific inhibitor psalmotoxin 1 pctx1 - by Bioz Stars, 2023-10
    86/100 stars

    Images

    1) Product Images from "ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction"

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    Journal: Physiological Research

    doi: 10.33549/physiolres.934950

    Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Figure Legend Snippet: Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Techniques Used: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.
    Figure Legend Snippet: Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    asic1a specific inhibitor psalmotoxin 1 pctx1  (ApexBio)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    ApexBio asic1a specific inhibitor psalmotoxin 1 pctx1
    Exposure to an acidic medium made the expression of <t>ASIC1a</t> in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Asic1a Specific Inhibitor Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1a specific inhibitor psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic1a specific inhibitor psalmotoxin 1 pctx1 - by Bioz Stars, 2023-10
    86/100 stars

    Images

    1) Product Images from "ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction"

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    Journal: Physiological Research

    doi: 10.33549/physiolres.934950

    Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Figure Legend Snippet: Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Techniques Used: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.
    Figure Legend Snippet: Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    psalmotoxin 1 pctx1  (ApexBio)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    ApexBio psalmotoxin 1 pctx1
    Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells. A1 and A2 Expression levels of ASIC1α in different group cells were detected by western blotting. The concentrations of <t>PcTx1</t> were 10, 20, 40 nM at 24 h. B1 , B2 , C1 and C2 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 for 6 h before performing wound healing assays. D1 and D2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 for 6 h before performing transwell invasion assays. E1 , E2 , E3 and E4 proliferation abilities of different group cells were determined by MTT assay. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group or PcTx1(0 nM) group
    Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psalmotoxin 1 pctx1 - by Bioz Stars, 2023-10
    86/100 stars

    Images

    1) Product Images from "ASIC1α up-regulates MMP-2/9 expression to enhance mobility and proliferation of liver cancer cells via the PI3K/AKT/mTOR pathway"

    Article Title: ASIC1α up-regulates MMP-2/9 expression to enhance mobility and proliferation of liver cancer cells via the PI3K/AKT/mTOR pathway

    Journal: BMC Cancer

    doi: 10.1186/s12885-022-09874-w

    Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells. A1 and A2 Expression levels of ASIC1α in different group cells were detected by western blotting. The concentrations of PcTx1 were 10, 20, 40 nM at 24 h. B1 , B2 , C1 and C2 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 for 6 h before performing wound healing assays. D1 and D2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 for 6 h before performing transwell invasion assays. E1 , E2 , E3 and E4 proliferation abilities of different group cells were determined by MTT assay. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group or PcTx1(0 nM) group
    Figure Legend Snippet: Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells. A1 and A2 Expression levels of ASIC1α in different group cells were detected by western blotting. The concentrations of PcTx1 were 10, 20, 40 nM at 24 h. B1 , B2 , C1 and C2 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 for 6 h before performing wound healing assays. D1 and D2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 for 6 h before performing transwell invasion assays. E1 , E2 , E3 and E4 proliferation abilities of different group cells were determined by MTT assay. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group or PcTx1(0 nM) group

    Techniques Used: Activity Assay, Expressing, Western Blot, Migration, MTT Assay

    Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells by down-regulating the expression of MMP-2 and MMP-9. A1 , A2 and A3 Expression levels of MMP-2 and MMP-9 in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, and the concentration of cis-ACCP was 20 µM at 24 h. B1 , B2 and B3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing wound healing assays. C1 and C2 Invasion abilities of different group cells were determined by transwell invasion assays. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing transwell invasion assays. Representative images were taken at × 200 magnification. D proliferation abilities of different group cells were determined by MTT assay. MTT assays were conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05 and *** P < 0.001 all versus pH6.5 group
    Figure Legend Snippet: Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells by down-regulating the expression of MMP-2 and MMP-9. A1 , A2 and A3 Expression levels of MMP-2 and MMP-9 in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, and the concentration of cis-ACCP was 20 µM at 24 h. B1 , B2 and B3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing wound healing assays. C1 and C2 Invasion abilities of different group cells were determined by transwell invasion assays. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing transwell invasion assays. Representative images were taken at × 200 magnification. D proliferation abilities of different group cells were determined by MTT assay. MTT assays were conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05 and *** P < 0.001 all versus pH6.5 group

    Techniques Used: Activity Assay, Expressing, Western Blot, Concentration Assay, Migration, MTT Assay

    ASIC1a can regulate aberrant activation of the PI3K/AKT/MTOR pathway. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of p-PI3Kp85, p-mTOR(Ser2448) and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, the concentration of LY294002 was 1 µM, the concentration of Rapamycin was 0.5 nM, the concentration of MK-2206 was 0.1 µM, and all conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group
    Figure Legend Snippet: ASIC1a can regulate aberrant activation of the PI3K/AKT/MTOR pathway. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of p-PI3Kp85, p-mTOR(Ser2448) and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, the concentration of LY294002 was 1 µM, the concentration of Rapamycin was 0.5 nM, the concentration of MK-2206 was 0.1 µM, and all conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group

    Techniques Used: Activation Assay, Expressing, Western Blot, Concentration Assay

    Overexpression of MMP-2/9 and activation of AKT can rescue the effect of reduced HepG2 and SK-Hep1 cells migration, invasion and proliferation caused by inhibiting ASIC1α. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of MMP-2, MMP-9 and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8, 16 µM at 24 h. D1 , D2 and D3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or SC79 for 6 h before performing wound healing assays. E1 and E2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or SC79 for 6 h before performing transwell invasion assays. F proliferation abilities of different group cells were determined by MTT assay. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8 µM at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus Control group
    Figure Legend Snippet: Overexpression of MMP-2/9 and activation of AKT can rescue the effect of reduced HepG2 and SK-Hep1 cells migration, invasion and proliferation caused by inhibiting ASIC1α. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of MMP-2, MMP-9 and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8, 16 µM at 24 h. D1 , D2 and D3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or SC79 for 6 h before performing wound healing assays. E1 and E2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or SC79 for 6 h before performing transwell invasion assays. F proliferation abilities of different group cells were determined by MTT assay. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8 µM at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus Control group

    Techniques Used: Over Expression, Activation Assay, Migration, Expressing, Western Blot, Concentration Assay, MTT Assay

    psalmotoxin 1 pctx1  (ApexBio)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    ApexBio psalmotoxin 1 pctx1
    Morphology of PSCs at pH 7.4 medium (A), pH 6.5 medium (B) or pretreatment with <t>PcTx1</t> and then culture in acid medium (C) was observed (400 X).
    Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psalmotoxin 1 pctx1 - by Bioz Stars, 2023-10
    86/100 stars

    Images

    1) Product Images from "Retracted Article: ASIC1a involves acidic microenvironment-induced activation and autophagy of pancreatic stellate cells"

    Article Title: Retracted Article: ASIC1a involves acidic microenvironment-induced activation and autophagy of pancreatic stellate cells

    Journal: RSC Advances

    doi: 10.1039/c8ra03679a

    Morphology of PSCs at pH 7.4 medium (A), pH 6.5 medium (B) or pretreatment with PcTx1 and then culture in acid medium (C) was observed (400 X).
    Figure Legend Snippet: Morphology of PSCs at pH 7.4 medium (A), pH 6.5 medium (B) or pretreatment with PcTx1 and then culture in acid medium (C) was observed (400 X).

    Techniques Used:

    Expression of ASIC1a in PSCs in different culture condition was assessed by western blotting and qRT-PCR. Western blotting results show that ASIC1a protein is expressed in PSC, and extracellular acid could increase its expression, but PcTx1 or siRNA knockdown of ASIC1a could inhibit the acid-induced expression of ASIC1a (A and B), which are consistent with the results of ASIC1a mRNA detected by qRT-PCR (C). Data are expressed as mean ± SD of three independent experiments. * P < 0.05 versus pH 6.5 group.
    Figure Legend Snippet: Expression of ASIC1a in PSCs in different culture condition was assessed by western blotting and qRT-PCR. Western blotting results show that ASIC1a protein is expressed in PSC, and extracellular acid could increase its expression, but PcTx1 or siRNA knockdown of ASIC1a could inhibit the acid-induced expression of ASIC1a (A and B), which are consistent with the results of ASIC1a mRNA detected by qRT-PCR (C). Data are expressed as mean ± SD of three independent experiments. * P < 0.05 versus pH 6.5 group.

    Techniques Used: Expressing, Western Blot, Quantitative RT-PCR

    ASIC1a involves the acid-induced activation of PSCs. The expression of α-SMA (A–C) and collagen 1 (D–F) at protein and mRNA level in PSCs in pH 6.5 medium both increase compared with that in pH 7.4 medium, but PcTx1 or siRNA knockdown of ASIC1a could reduce the enhanced expression of α-SMA and collagen 1. Data are expressed as mean ± SD of three independent experiments. * P < 0.05 versus pH 6.5 group.
    Figure Legend Snippet: ASIC1a involves the acid-induced activation of PSCs. The expression of α-SMA (A–C) and collagen 1 (D–F) at protein and mRNA level in PSCs in pH 6.5 medium both increase compared with that in pH 7.4 medium, but PcTx1 or siRNA knockdown of ASIC1a could reduce the enhanced expression of α-SMA and collagen 1. Data are expressed as mean ± SD of three independent experiments. * P < 0.05 versus pH 6.5 group.

    Techniques Used: Activation Assay, Expressing

    ASIC1a involves the acid-induced expression of beclin 1 in PSC. The semiquantitative analyses of the western blotting gels (A and B) and the results of qRT-PCR (C) both show that, compared with pH 7.4 group, an up-regulated expression of beclin 1 occurred at protein and mRNA level in PSCs in pH 6.5 group, which can be suppressed by PcTx1 or siRNA knockdown of ASIC1a. Data are expressed as mean ± SD of three independent experiments. * P < 0.05 versus pH 6.5 group.
    Figure Legend Snippet: ASIC1a involves the acid-induced expression of beclin 1 in PSC. The semiquantitative analyses of the western blotting gels (A and B) and the results of qRT-PCR (C) both show that, compared with pH 7.4 group, an up-regulated expression of beclin 1 occurred at protein and mRNA level in PSCs in pH 6.5 group, which can be suppressed by PcTx1 or siRNA knockdown of ASIC1a. Data are expressed as mean ± SD of three independent experiments. * P < 0.05 versus pH 6.5 group.

    Techniques Used: Expressing, Western Blot, Quantitative RT-PCR

    The protein expression of LC3B II and LC3B I in PSCs with different treatment. Results show the expression of LC3B II increases and the expression of LC3B I decreases after PSCs treated with acid, but remained constant when PSCs were pretreated with PcTx1 or siRNA knockdown of ASIC1a. * ,# P < 0.05 versus the expression of LC3B I and LC3B II in pH 6.5 group, respectively.
    Figure Legend Snippet: The protein expression of LC3B II and LC3B I in PSCs with different treatment. Results show the expression of LC3B II increases and the expression of LC3B I decreases after PSCs treated with acid, but remained constant when PSCs were pretreated with PcTx1 or siRNA knockdown of ASIC1a. * ,# P < 0.05 versus the expression of LC3B I and LC3B II in pH 6.5 group, respectively.

    Techniques Used: Expressing

    psalmotoxin 1 pctx1  (ApexBio)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    ApexBio psalmotoxin 1 pctx1
    Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psalmotoxin 1 pctx1 - by Bioz Stars, 2023-10
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • asic1a specific inhibitor psalmotoxin 1 pctx1  (ApexBio)
    86
    ApexBio asic1a specific inhibitor psalmotoxin 1 pctx1
    Exposure to an acidic medium made the expression of <t>ASIC1a</t> in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Asic1a Specific Inhibitor Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1a specific inhibitor psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic1a specific inhibitor psalmotoxin 1 pctx1 - by Bioz Stars, 2023-10
    86/100 stars
    		  Buy from Supplier
    psalmotoxin 1 pctx1  (ApexBio)
    86
    ApexBio psalmotoxin 1 pctx1
    Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells. A1 and A2 Expression levels of ASIC1α in different group cells were detected by western blotting. The concentrations of <t>PcTx1</t> were 10, 20, 40 nM at 24 h. B1 , B2 , C1 and C2 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 for 6 h before performing wound healing assays. D1 and D2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 for 6 h before performing transwell invasion assays. E1 , E2 , E3 and E4 proliferation abilities of different group cells were determined by MTT assay. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group or PcTx1(0 nM) group
    Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psalmotoxin 1 pctx1 - by Bioz Stars, 2023-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells. A1 and A2 Expression levels of ASIC1α in different group cells were detected by western blotting. The concentrations of PcTx1 were 10, 20, 40 nM at 24 h. B1 , B2 , C1 and C2 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 for 6 h before performing wound healing assays. D1 and D2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 for 6 h before performing transwell invasion assays. E1 , E2 , E3 and E4 proliferation abilities of different group cells were determined by MTT assay. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group or PcTx1(0 nM) group

    Journal: BMC Cancer

    Article Title: ASIC1α up-regulates MMP-2/9 expression to enhance mobility and proliferation of liver cancer cells via the PI3K/AKT/mTOR pathway

    doi: 10.1186/s12885-022-09874-w

    Figure Lengend Snippet: Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells. A1 and A2 Expression levels of ASIC1α in different group cells were detected by western blotting. The concentrations of PcTx1 were 10, 20, 40 nM at 24 h. B1 , B2 , C1 and C2 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 for 6 h before performing wound healing assays. D1 and D2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 for 6 h before performing transwell invasion assays. E1 , E2 , E3 and E4 proliferation abilities of different group cells were determined by MTT assay. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group or PcTx1(0 nM) group

    Article Snippet: A potent and selective ASIC1α blocker, Psalmotoxin 1 (PcTx1) (#B5796), and a type IV collagen-specific MMP-2 and MMP-9 inhibitor, cis-ACCP (#C4501), were purchased from APExBIO Technology LLC (Houston, TX, USA).

    Techniques: Activity Assay, Expressing, Western Blot, Migration, MTT Assay

    Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells by down-regulating the expression of MMP-2 and MMP-9. A1 , A2 and A3 Expression levels of MMP-2 and MMP-9 in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, and the concentration of cis-ACCP was 20 µM at 24 h. B1 , B2 and B3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing wound healing assays. C1 and C2 Invasion abilities of different group cells were determined by transwell invasion assays. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing transwell invasion assays. Representative images were taken at × 200 magnification. D proliferation abilities of different group cells were determined by MTT assay. MTT assays were conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05 and *** P < 0.001 all versus pH6.5 group

    Journal: BMC Cancer

    Article Title: ASIC1α up-regulates MMP-2/9 expression to enhance mobility and proliferation of liver cancer cells via the PI3K/AKT/mTOR pathway

    doi: 10.1186/s12885-022-09874-w

    Figure Lengend Snippet: Inhibiting the activity of ASIC1α reduces the mobility and proliferation of HepG2 and SK-Hep1 Cells by down-regulating the expression of MMP-2 and MMP-9. A1 , A2 and A3 Expression levels of MMP-2 and MMP-9 in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, and the concentration of cis-ACCP was 20 µM at 24 h. B1 , B2 and B3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing wound healing assays. C1 and C2 Invasion abilities of different group cells were determined by transwell invasion assays. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or cis-ACCP for 6 h before performing transwell invasion assays. Representative images were taken at × 200 magnification. D proliferation abilities of different group cells were determined by MTT assay. MTT assays were conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05 and *** P < 0.001 all versus pH6.5 group

    Article Snippet: A potent and selective ASIC1α blocker, Psalmotoxin 1 (PcTx1) (#B5796), and a type IV collagen-specific MMP-2 and MMP-9 inhibitor, cis-ACCP (#C4501), were purchased from APExBIO Technology LLC (Houston, TX, USA).

    Techniques: Activity Assay, Expressing, Western Blot, Concentration Assay, Migration, MTT Assay

    ASIC1a can regulate aberrant activation of the PI3K/AKT/MTOR pathway. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of p-PI3Kp85, p-mTOR(Ser2448) and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, the concentration of LY294002 was 1 µM, the concentration of Rapamycin was 0.5 nM, the concentration of MK-2206 was 0.1 µM, and all conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group

    Journal: BMC Cancer

    Article Title: ASIC1α up-regulates MMP-2/9 expression to enhance mobility and proliferation of liver cancer cells via the PI3K/AKT/mTOR pathway

    doi: 10.1186/s12885-022-09874-w

    Figure Lengend Snippet: ASIC1a can regulate aberrant activation of the PI3K/AKT/MTOR pathway. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of p-PI3Kp85, p-mTOR(Ser2448) and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, the concentration of LY294002 was 1 µM, the concentration of Rapamycin was 0.5 nM, the concentration of MK-2206 was 0.1 µM, and all conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group

    Article Snippet: A potent and selective ASIC1α blocker, Psalmotoxin 1 (PcTx1) (#B5796), and a type IV collagen-specific MMP-2 and MMP-9 inhibitor, cis-ACCP (#C4501), were purchased from APExBIO Technology LLC (Houston, TX, USA).

    Techniques: Activation Assay, Expressing, Western Blot, Concentration Assay

    Overexpression of MMP-2/9 and activation of AKT can rescue the effect of reduced HepG2 and SK-Hep1 cells migration, invasion and proliferation caused by inhibiting ASIC1α. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of MMP-2, MMP-9 and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8, 16 µM at 24 h. D1 , D2 and D3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or SC79 for 6 h before performing wound healing assays. E1 and E2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or SC79 for 6 h before performing transwell invasion assays. F proliferation abilities of different group cells were determined by MTT assay. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8 µM at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus Control group

    Journal: BMC Cancer

    Article Title: ASIC1α up-regulates MMP-2/9 expression to enhance mobility and proliferation of liver cancer cells via the PI3K/AKT/mTOR pathway

    doi: 10.1186/s12885-022-09874-w

    Figure Lengend Snippet: Overexpression of MMP-2/9 and activation of AKT can rescue the effect of reduced HepG2 and SK-Hep1 cells migration, invasion and proliferation caused by inhibiting ASIC1α. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of MMP-2, MMP-9 and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8, 16 µM at 24 h. D1 , D2 and D3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. The cells were treated with PcTx1 or SC79 for 6 h before performing wound healing assays. E1 and E2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. Invasion assays were conducted at 24 h. The cells were treated with PcTx1 or SC79 for 6 h before performing transwell invasion assays. F proliferation abilities of different group cells were determined by MTT assay. The concentration of PcTx1 was 20 nM and the concentration of SC79 was 8 µM at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus Control group

    Article Snippet: A potent and selective ASIC1α blocker, Psalmotoxin 1 (PcTx1) (#B5796), and a type IV collagen-specific MMP-2 and MMP-9 inhibitor, cis-ACCP (#C4501), were purchased from APExBIO Technology LLC (Houston, TX, USA).

    Techniques: Over Expression, Activation Assay, Migration, Expressing, Western Blot, Concentration Assay, MTT Assay